Sterilization of Test Brew

filhodaputa

Banned
Testosterone sterilization instructions from Medisca for compounding pharmacies states to use a dry heat chamber for 120 minutes at 160C for the finished product . I will upload a copy of the document when I get access to my computer.
 
Last edited:
Testosterone sterilization instructions from Medisca for compounding pharmacies states to use a dry heat chamber for 120 minutes at 160C for the finished product . I will upload a copy of the document when I get access to my computer.
That's standard terminal heat sterilization used by compounding pharmacies that work with USP grade raws, NOT the kitchen cook with Chinese raws and Walmart bought oils
 
Why would the sterilization process be any different? I understand filtering impurities is one thing, but sterilization should be the same no matter what. Wouldn’t the heat address sterilization regardless of raw source?
 
The home brewer may have more control of his surroundings. Big pharma has to work under the biggest idiot theory. They have rules in place to double check in case of what the stupidest person that works for them may do when hurrying through their job while thinking about what they will be doing when they get off work and forgetting procedures that are in place to guarantee sterility.
 
The home brewer may have more control of his surroundings. Big pharma has to work under the biggest idiot theory. They have rules in place to double check in case of what the stupidest person that works for them may do when hurrying through their job while thinking about what they will be doing when they get off work and forgetting procedures that are in place to guarantee sterility.
This is a very good point
Most large workplaces are like this. Procedures and policies regarding safety and contamination or exposure are designed for and governed by what the stupidest person might do. Often revisions come about when a new stupidest person works their magic.
 
The home brewer may have more control of his surroundings.
Oh, like when he pulls the vials out of the oven after sterilizing, then sets them on the kitchen countertop in a unsterilized room, then bottles the finished oil into them.....

Not that I think its a major issue but saying that a home brewer has more control then a dedicated compounding lab is drawing a loooong bow.
 
Testosterone sterilization instructions from Medisca for compounding pharmacies states to use a dry heat chamber for 120 minutes at 160C for the finished product . I will upload a copy of the document when I get access to my computer.
Not the same but after seeing someone using a pressure cooker in the home brew section here, I've started using one as a post bottling sterilisation step.

So I still cook as per normal, filter and bottle. Then put the finished and capped bottles into a pressure cooker on high for a 45min cycle.

Originally I started doing it to help stabilize some high dose brews that had a tendency to crash (which it certainly has helped with) but then figured it was a easy sterilization step as well.
 
Oh, like when he pulls the vials out of the oven after sterilizing, then sets them on the kitchen countertop in a unsterilized room, then bottles the finished oil into them.....

Not that I think its a major issue but saying that a home brewer has more control then a dedicated compounding lab is drawing a loooong bow.
Yes just like that as he could have sterilized the counter if he choose and knows he did it. Where as in the lab he has no idea if someone actually did that or not and has to have trust in strangers that are only doing their job. And don't have their own health in the balance. My x wife was a research scientist so i know how well things may be sterile as i spent time in those labs. And in a large commercial manufacturing plant i think it would be even less sterile. But we all have our own opinion. I am just going off my 20+ years of home brewing.
 
Have anyone of you seen how a pharma manufacturing line of injectibles setup?

Automated lines in HEPA filtered air controlled rooms. They are NOT the average scientific lab setup with people walking in white coats.

And no, the kitchen sink homebrewer, with his 0.22μm filters doesn't have a more sterile environment, unless he has a 20k sealed room setup and uses gamma radiation for sterilization AFTER the 0.45μm fltration to remove contaminants from the brew.
 
Yes just like that as he could have sterilized the counter if he choose and knows he did it. Where as in the lab he has no idea if someone actually did that or not and has to have trust in strangers that are only doing their job. And don't have their own health in the balance. My x wife was a research scientist so i know how well things may be sterile as i spent time in those labs. And in a large commercial manufacturing plant i think it would be even less sterile. But we all have our own opinion. I am just going off my 20+ years of home brewing.
My point was that you can sterilize the fuck out of your benchtop and whatever else you want.... Your NOT in a sterile room and there is bacteria floating around in the AIR and getting into your open top vials after you have sterilized them!

The fact your X worked in the industry and you know how sloppy they can be WITHOUT major health effects for the end user, coupled with unsterile home brewers not running into major issues should give you a clear indication just how much of a non issue this actually is!
 
My point was that you can sterilize the fuck out of your benchtop and whatever else you want.... Your NOT in a sterile room and there is bacteria floating around in the AIR and getting into your open top vials after you have sterilized them!

The fact your X worked in the industry and you know how sloppy they can be WITHOUT major health effects for the end user, coupled with unsterile home brewers not running into major issues should give you a clear indication just how much of a non issue this actually is!
This being a non issue for most intelligent people was my point but you seemed to miss it.
 
This being a non issue for most intelligent people was my point but you seemed to miss it.
LOL, yeah I had missed that bro! Sorry! Same boat, been brewing since 2005 and just so used to seeing the same stuff regurgitated with no real world experience taken into account so I made an assumption in replying to you and I was wrong for doing that.
 
i think about 90% give or take use gear brewed on who knows what environment by your so called top ugls. I’m betting those guys are scratching their balls, doing farting contests and vaping vanilla bean gelato juice while semi filtering that Prop/tren/mast mix you oh so loved lol

There is no massive cases of infection yet from homebrewers or ugl gear users yet, so maybe that BA and filter really does work.
 
i think about 90% give or take use gear brewed on who knows what environment by your so called top ugls. I’m betting those guys are scratching their balls, doing farting contests and vaping vanilla bean gelato juice while semi filtering that Prop/tren/mast mix you oh so loved lol

There is no massive cases of infection yet from homebrewers or ugl gear users yet, so maybe that BA and filter really does work.
Yeah, you know you are 100% correct! No way 99% of the UGL's are churning out 1000's of finished vials and running those vials through sterilization before filling! Guarantee they are doing the cook, running the finished product through a 0.45 filter to get rid of any solids/floaters in the finished product and a 0.45 will filter reasonably quick, stripping slabs of vials that are all open topped and been sitting in storage/warehouse/shipping and laying those vials out ready to fill, then just going at it, cap then label and into stock ready to ship.

And we don't see ANY nasty infection's popping up!

There are "best practices" and then there is REALITY!

This is also why I used to filter Chinese finished oils whenever I got them back in the day, just for my own piece of mind. Its also what spurred me to start brewing my own, that way its my responsibility for what goes in my body.

0.45 removes the solids/floaters, BA kills off any bacteria.

No way is any home brew "sterile" and I'll guarantee 99% of UGL's are in the exact same boat! And it doesn't matter in the grand scheme of things!
 
Not the same but after seeing someone using a pressure cooker in the home brew section here, I've started using one as a post bottling sterilisation step.

So I still cook as per normal, filter and bottle. Then put the finished and capped bottles into a pressure cooker on high for a 45min cycle.

Originally I started doing it to help stabilize some high dose brews that had a tendency to crash (which it certainly has helped with) but then figured it was an easy sterilization step as well.
Per my research reading pharmaceutical compounding textbooks, this will not sterilize the contents inside the vial. Steam autoclaving uses a combination of both pressure and heat for sterilization. The contents inside the vial are not subjected to the pressure outside the vial, so the contents will not reach a sufficiently high temperature to be sterilized. This is due to the lack of pressure.

Terminal sterilization in vials, must use dry heat sterilization, which does not require high pressure, but will take much longer (120+ minutes ) due to the inefficiencies of dry heat transfer.

I can post the textbook references if anyone is interested.
 
@HereToTeachAndLearn
Please post the textbook references, I am curious.
As far as I know the pressure in the autoclave is just the mechanism to achieve boiling water with 121 degrees. The steam and heat conduction of 121 degrees do the actual sterilisation by killing the microbes. Hence why you could also use a pressure cooker.
 
Yeah, you know you are 100% correct! No way 99% of the UGL's are churning out 1000's of finished vials and running those vials through sterilization before filling! Guarantee they are doing the cook, running the finished product through a 0.45 filter to get rid of any solids/floaters in the finished product and a 0.45 will filter reasonably quick, stripping slabs of vials that are all open topped and been sitting in storage/warehouse/shipping and laying those vials out ready to fill, then just going at it, cap then label and into stock ready to ship.

And we don't see ANY nasty infection's popping up!

There are "best practices" and then there is REALITY!

This is also why I used to filter Chinese finished oils whenever I got them back in the day, just for my own piece of mind. Its also what spurred me to start brewing my own, that way its my responsibility for what goes in my body.

0.45 removes the solids/floaters, BA kills off any bacteria.

No way is any home brew "sterile" and I'll guarantee 99% of UGL's are in the exact same boat! And it doesn't matter in the grand scheme of things!
I agree completely. I don't think any ugl goes to the extent to be sterile as they claim. Yes they have autoclaves but probably only to clean glassware. They absolutely don't dunk and autoclave all the vials and stoppers and I think some ugls don't even use the 0.45, they just sell it like it is. I laugh when I see a ugls introduction claiming double filtered and all this bullshit lol
 
I agree completely. I don't think any ugl goes to the extent to be sterile as they claim. Yes they have autoclaves but probably only to clean glassware. They absolutely don't dunk and autoclave all the vials and stoppers and I think some ugls don't even use the 0.45, they just sell it like it is. I laugh when I see a ugls introduction claiming double filtered and all this bullshit lol
I'm actually gonna double filter. First line will be with a shit .45 micron syringe filter to remove most of the stuff. Then follow up with a whatman syringe filter into to a vial.
 
I'm actually gonna double filter. First line will be with a shit .45 micron syringe filter to remove most of the stuff. Then follow up with a whatman syringe filter into to a vial.
You don't need to do that. I was referring more to bottletop or in line filters. If you're just filtering your own homebrew then .22 Whatmans is all you need. One pass, warm oil and steady pressure. I personally don't do the whole 60ml and cawlk gun method because it's difficult to tell how much pressure I'm using. So I use 5ml syringes with a whatman. I can filter 10ml in about a minute, maybe two.

Before whatmans I used cheap Chinese no name syringe filters off ebay. Never got an infection.

The shit .45 might even put more junk into your brew so the whatman will have to be changed more
 
Before whatmans I used cheap Chinese no name syringe filters off ebay. Never got an infection.

The shit .45 might even put more junk into your brew so the whatman will have to be changed more
I kind of doubt that a cheaper filter would put more stuff in

Thats like saying the cheap chinese no name filter should have given you an infection because it would have added more junk to what should have been sterile.


but I am trying it out. maybe I can change the whatman out every 160ml instead of 90-100
 
Back
Top