Petarosus188
Member
About freezing....lets say I order premixed and lyophilized GH during winter. During storage and deliveries...it will be probably frozen and unfreeze later- is that a real problem for GH damage?
MESO-Rx
Anabolic Steroids
Storage conditions and dimer
- Thread starter janoshik
- Start date
janoshik
Subscriber
It doesn't notably affect lyophilized (not dissolved) hormone.
Petarosus188
Member
OK so liquid ones, might not be a good idea?
janoshik
Subscriber
It's preferable to avoid having those subjected to freezing conditions.
It also might be worth noting that a mixture, such as dissolved somatropin preparation will freeze at temperatures significantly lower than pure water.
This is a great thread and a really interesting test.
So the general concensus seems to be that room temperature in a dark place seems to be OK for non-reconstituted GH.
Would storing non-reconstituted GH in the fridge yield ANY positive benefits over room temperature in a dark place, even If its only a 1-3% benefit?
So the general concensus seems to be that room temperature in a dark place seems to be OK for non-reconstituted GH.
Would storing non-reconstituted GH in the fridge yield ANY positive benefits over room temperature in a dark place, even If its only a 1-3% benefit?
janoshik
Subscriber
Well, we do store stuff in the fridge long term.
This comes as a relief - as it turns out, wives don't really appreciate you filling the refrigerator with endless vials of PEDs.
F that. Claim your shelf on the side door Brother lol!
Ghoul
Member
Jano,
I came across a study of HGH degradation in which, after some severe stress (low PH and 37c temps) of two samples, purity tested at 98% and 96%. I suspect they were surprised by this, and realized when comparing the larger peak area of the initial unstressed sample, that the insoluble and poorly soluble aggregates HGH is known to produce weren't eluting from the the column.
After compensating for this, purity was actually 68% and 81%,
Is this something you think should be applied to future stress testing? I could be mistaken, but I thought I saw results in which peptides had the same (or similar) purity before and after stressing, but the mass dropped, which sounds similar to what the researchers encountered here.
As far as I know the fact insoluble aggregates don't elute doesn't seem to be a factor considered by any of the labs in the UGL space.
Thanks in advance.
(bottom left page 49)
I came across a study of HGH degradation in which, after some severe stress (low PH and 37c temps) of two samples, purity tested at 98% and 96%. I suspect they were surprised by this, and realized when comparing the larger peak area of the initial unstressed sample, that the insoluble and poorly soluble aggregates HGH is known to produce weren't eluting from the the column.
After compensating for this, purity was actually 68% and 81%,
Is this something you think should be applied to future stress testing? I could be mistaken, but I thought I saw results in which peptides had the same (or similar) purity before and after stressing, but the mass dropped, which sounds similar to what the researchers encountered here.
As far as I know the fact insoluble aggregates don't elute doesn't seem to be a factor considered by any of the labs in the UGL space.
Thanks in advance.
(bottom left page 49)
janoshik
Subscriber
Correct!
Turbidimetry should be able to see such aggregates.
It's on my list to check once less busy, but we're not able to hire people fast enough.
About "hgh stressing" If I kept a large amount of GH in room temp with no light for a few months (2-4 months), would it be worth it to tranfer all of them to the fridge now or are there contraindications after a certain amount of time ? I received a brand new batch of the same concentration (claimed, at least), and it seems to hit harder than the one kept at room temp, making me want to put all my peptides in the fridge. Can't tell how much they lost in potency though.
janoshik
Subscriber
there's no such contraindication
Off topic... But I'm from Europe like you and I have access to unlimited saline (pH 5.5), but as you know, not Bac water. Can I use saline without any problems for GH and peptides or do you see any problem with that? Thank you very much sir
Ghoul
Member
Without a bacteriostatic component, it's not safe to use for more than 24 hours after reconstitution. Jano's found a fairly high percentage of peptides failed sterility tests, not unexpected, and that bacteria feeds on protein. It's like you're reconstituting powdered eggs. You could add Benzyl Alcohol in the correct proportion. I assume you're in the EU and can't source BAC water?
Ghoul
Member
Control what you can, what's done is done. If you're keeping it, the sooner you get it in the refrigerator, the less degradation it'll experience. It would take extensive testing of that specific batch to know how fast it degenerates, but we know whatever it is, it's *less* in cold temps.
Also, reconstitute to 3ml. When HGH is damaged, and it starts to aggregate, experiments show the greater the concentration, the worse the aggregation is because, simply put, the molecules are crowded and bump into each other more, increasing the chance of sticking to each other.
Here's an illustration of how damaged (oxidized in this case) HGH forms more and larger aggregates with increased concentration:
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Makes sense. Another reason to only use injectable water if you inject the peptide right away
Ghoul
Member
I forgot to mention to mention, the salt in saline also speeds up degradation of HGH (and probably many if not all peptides).
Yes, as I wrote, I'm from the EU.. So, does 0.9% saline solution protect against bacteria for a longer period of time?
Yeah, i noticed that some injectable water some sources give you for free is saline or something else. Cannot tell for certain but noticed that sometimes HCG i use got rock solid over night in the fridge even when diluting it with 3ml or more which makes me wonder if that could have been the case here then
I hear it for the first time... that doesn't sound good
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