Storage conditions for lyophilized peptides

[Lazarev]

Hello everyone,

First post here, been lurking for a long time and I'm finally getting started on a cycle. I work in pharma/medical devices so I'm very knowledgeable when it comes to most things related, including manufacturing, but peptides have me completely dumbfounded.

I see very varying opinions and data on how lyophilized peptides should be stored. And on top of that it depends on the peptide too. From what I understand HGH is decently stable lyophilized as long as the temp doesn't change too much and it's away from light/moisture. But considering it's UGL it obviously won't be as stable as pharma-grade so it's most likely less.

While I agree that obviously reconstituted peptides should be stored in the fridge and used short-term, for lyophilized, some say drawer is fine, most people recommend to store it in the freezer, but wouldn't that increase the risk of moisture getting in and degrading the peptide/forming aggregates ?

I have Retatrutide and HCG on hand, so I'm wondering how I should store it for the best effectiveness ? Even if they'll be used in a relatively short timespan (~3 months, not much into stocking up until I'm sure about everything related to safety/storage/usage/effectiveness etc)

If you have any data, research and such, fully open to discussing, learning and seeing what you have

Thanks a lot ! <3

 

[AlexDavis43]

Meanwhile serostim is stored at room temp lol

1. Serostim is stored at room temp, has higher aggregates ("dimer"), mediocre purity (mid-90's), and great anecdotal results

2. Lobster's UGL vacuumless 50 IU vial showing no degradation at 6 months

3. Some of the best IGF1 responses from guys using non-filtered GH

4. Study showing GH with high aggregates doesn't cause immunogenicity or reduced GH efficacy

Conclusion: I think we're making progress in understanding things in the real world, as opposed to the professors' theories based on "the world's most renowned peptide gurus" and FDA/Pharma powerpoint presentations

 

[LawrenceofUSA]

Mini fridge in the bedroom. Fits right on the nightstand next to the bed. Make it a lot easier for pre bed administration. The in-laws come over a lot, and know they are welcome to help themselves, this prevents any awkward conversation not being in the kitchen fridge.

Amazon.com

Hey man, looking at getting your fridge recommendation. Was wondering a couple things.

1) do you turn it all the way down to keep it as cold as it will go or what?

2) have any issues at all with humidity or condensation?

3) do you store the kits in any sort of box with foam inserts to prevent agitation or anything of the sort?

4) have you stored retatrutide specifically long term? How long are you noticing the peptides you are using staying good using this method?

Thanks brother

 

[HUGE McBIGLARGE]

in my opinion:
A fancy freezer with individually insulated vials > fridge > normal freezer with non-insulated vials

The problem with a freezer is if you don’t have insulated vials, they’ll warm up to above freezing very quickly when exposed to room-temp air. The vials and their contents are low mass. So you’ve either gotta be very quick at getting the vials out, or insulate them. If not, they’ll go through freeze-thaw cycles, possibly fucking up the contents.

I keep mine in a lunchbox in the fridge. Seems better than a freezer in my opnion

But I’m not very educated on this. Perhaps someone else thinks the possibility of freeze-thaw cycles is preferable to the warmer temperature of the fridge.

 

[Spaceman Spiff]

1. Serostim is stored at room temp, has higher aggregates ("dimer"), mediocre purity (mid-90's), and great anecdotal results

2. Lobster's UGL vacuumless 50 IU vial showing no degradation at 6 months

3. Some of the best IGF1 responses from guys using non-filtered GH

4. Study showing GH with high aggregates doesn't cause immunogenicity or reduced GH efficacy

Conclusion: I think we're making progress in understanding things in the real world, as opposed to the professors' theories based on "the world's most renowned peptide gurus" and FDA/Pharma powerpoint presentations

Forgot to bring up this important thing..


Hiv patients inject 18iu daily.(1 vial)


With all the cumulative exposure to aggregates.... It doesn't mean shit.

 

[HUGE McBIGLARGE]

The problem with a freezer is if you don’t have insulated vials, they’ll warm up to above freezing very quickly when exposed to room-temp air. The vials and their contents are low mass. So you’ve either gotta be very quick at getting the vials out, or insulate them. If not, they’ll go through freeze-thaw cycles, possibly fucking up the contents.

I’ve started storing them in the freezer now. I keep about 3 months worth in the fridge, with the rest in a lunchbox in the freezer. I figure having to dip into the lunchbox every 3 months isn’t so bad if I’m fast enough.

Obviously the lunchbox is full of silica packs.

 

[CubanQuentin]

I’ve started storing them in the freezer now. I keep about 3 months worth in the fridge, with the rest in a lunchbox in the freezer. I figure having to dip into the lunchbox every 3 months isn’t so bad if I’m fast enough.

Obviously the lunchbox is full of silica packs.

Sounds like the lunchbox is a better choice than a minifridge, then

As my 2 cents on the used fridge/freezer thing I'd say that I wouldn't touch one with a 10 ft pole. The thermostat or the compressor could be compromised and you don't even realize until a month later when your energy consumption has shot up (ask me how I know). Not sure if the same principle applies to a cryofreezer, but given that you should pretty much only use one for long-term storage of potentially 1000s of $ of stuff, it can't be that bad of an idea to spend the extra cash on a new one. You'll never know the conditions that a used freezer has been through

 

[Spaceman Spiff]

I’ve started storing them in the freezer now. I keep about 3 months worth in the fridge, with the rest in a lunchbox in the freezer. I figure having to dip into the lunchbox every 3 months isn’t so bad if I’m fast enough.

Obviously the lunchbox is full of silica packs.

What are the silica packs supposed to do to airtight vials?

 

[Ghoul]

Sounds like the lunchbox is a better choice than a minifridge, then

As my 2 cents on the used fridge/freezer thing I'd say that I wouldn't touch one with a 10 ft pole. The thermostat or the compressor could be compromised and you don't even realize until a month later when your energy consumption has shot up (ask me how I know). Not sure if the same principle applies to a cryofreezer, but given that you should pretty much only use one for long-term storage of potentially 1000s of $ of stuff, it can't be that bad of an idea to spend the extra cash on a new one. You'll never know the conditions that a used freezer has been through

That's fair. But the portable cryofreezers are made for EMS use and extreme durability. When you turn them on they go through a self test cycle, warn of any temp variability, keep logs, etc . These aren't household minifridges. Something the size of a small portable cooler with a $10,000 list price, and built like it. The places that sell them are usually medical equipment suppliers, typically say they're inspected, offer a warranty etc.

The Chinese clones are available brand new for roughly the same price as the used US made portables . They look good, some models go down to -100c, but durability is a question mark I guess. They also offer "lab" style units, that look more the a small refrigerator. Same cryo specs, without the portability, ability to work off a 12v battery etc, but more storage room for the same price.

 

[CubanQuentin]

What are the silica packs supposed to do to airtight vials?

You store them there in case your gear goes bad and you decide to leave the simulation

Spoiler

 

[Spaceman Spiff]

You store them there in case your gear goes bad and you decide to leave the simulation

Spoiler

View attachment 330415

I'm not sure if he understands that products in an airtight container won't be affected by silica on the outside of it

 

[HUGE McBIGLARGE]

What are the silica packs supposed to do to airtight vials?

Any microscopic ice that forms on the rubber stoppers could damage them.

And who’s to say all the vials are airtight? Manufacturing errors do occur

If I’m storing £200 of peptides, I’m including a £0.30 silica pack.

 

[Ghoul]

Any microscopic ice that forms on the rubber stoppers could damage them.

And who’s to say all the vials are airtight? Manufacturing errors do occur

If I’m storing £200 of peptides, I’m including a £0.30 silica pack.

Wise move for any Lobster customers...

 

[AlexDavis43]

Any microscopic ice that forms on the rubber stoppers could damage them.

And who’s to say all the vials are airtight? Manufacturing errors do occur

If I’m storing £200 of peptides, I’m including a £0.30 silica pack.

If they're not airtight, how long you plan on storing them for?

Anything that's going in for 5+ years (doomsday stash), better hope they're airtight.

 

[HUGE McBIGLARGE]

If they're not airtight, how long you plan on storing them for?

Anything that's going in for 5+ years (doomsday stash), better hope they're airtight.

What I mean is that while the vials are designed to be airtight, there’s the chance the airtight seal could break without you knowing.

In such an event, it’s better to have desiccated my drug lunchbox than to have not.

 

[HUGE McBIGLARGE]

I’m now keeping a thermometer on top of my drug lunchbox. It has a min/max record. If the max goes above 0c I know my peptides are at risk of freeze/thaw cycling, and I’ll switch them to a non-frost-free freezer.

 

[HUGE McBIGLARGE]

So far the thermometer isn’t reporting a max temp above -11c. So that’s good.

Based on my research, it seems that for long-term storage, keeping them below 0c is the most important thing. Apparently a good rule is that for every 10 degrees colder peptides are, they have 2.5x the stability.

This means that an hour at 20c will degrade peptides more than it would 24 hours at -20c. And an hour at 20c will degrade peptides more than it would a year at -80c.

So if your peptides take 5 months to go from manufacture to your -20c freezer, and you stored your peptides for 10 years before using them, then 50-66% of the degradation occurred before you froze them.

 

[Sampei]

So far the thermometer isn’t reporting a max temp above -11c. So that’s good.

Based on my research, it seems that for long-term storage, keeping them below 0c is the most important thing. Apparently a good rule is that for every 10 degrees colder peptides are, they have 2.5x the stability.

This means that an hour at 20c will degrade peptides more than it would 24 hours at -20c. And an hour at 20c will degrade peptides more than it would a year at -80c.

So if your peptides take 5 months to go from manufacture to your -20c freezer, and you stored your peptides for 10 years before using them, then 50-66% of the degradation occurred before you froze them.

Yeah all good and nice but without data on how much it degrades ar 20c this whole speech is just mental masturbation.

Spoiler alert, doesn't degrade for shit at room temperature.

20+ of the most known peptides have been tested at a constant temperature of 130f for 21 days and the degradation was negligible on 95+% of those.

So yeah nice mental jerk off

 

[Turkeybuilder]

ive used the same vial of melanotan 2 for 5 months that ive never refrigerated and kept in a drawer

guess what i'm still bronze as fuck and still nauseous each pin

 

[Albertaboy73]

I keep my long term storage BPC157,TB500,MotsC,Retatrutide in the Freezer @-18. My HGH is stored in the fridge. Monthly used PEPS I keep in the fridge. Anything sitting over a month freezer. I researched and got the numbers and then left it at that. I'm not pushing for -80 since most of my gear will be used in the next year.