THE new "Generic" HGH Assay PAGE! AAA testing

[ProfessorX]

Dr. Jim
I will go at this from another angle,
we have seen several, and based on the results posted, I wont even call them generic but rather bio similar HGH samples compared to the standard.
Will we see How a Pharma sample compares to the standard?
How close will the Pharma grade sample have to be to the standard to still be allow for use and sale as HGH by the FDA?
What would you xpect would b acceptable?

also I tried the science xchange route several times. no luck thus far.
this is there response to me from a few days ago......


Dear Buck,
My name is JR and I am a Customer Success Manager at Science Exchange. I am writing to follow-up on your request forAmino Acid Analysisservice that you've submitted to one of our service providers,Proteomics and Mass Spectrometry Facility.
Science Exchange is an online marketplace where scientists can outsource research services to our network of expert service providers. These research services typically encompasses scientific research in the pre-clinical and clinical pipeline.
The request that you have submitted presents several legal complications and Science Exchange is unable to support your project at this time. We cannot support and facilitate requests for services that are intended for personal use, therefore your request for quotation has been deleted from our platform.
If you would like to pursue this request, please provide me with the following information and our team can reassess the request:

1. Please provide me with your work affiliated email (not a Hotmail, Gmail, or Yahoo email address).
2. What is the intended use of the data or product? If it will be used for basic scientific research, please provide me with references to your past publications.

Please let me know if you have any questions.

Best regards,

JR

Buck,

I'm on the same page as you, just trying to learn more about the testing methods, process, etc

When I had some GH samples tested in the past, I had asked for "amount present" in each sample vial

After questioning the results (amount seemed off to me) the lab suggested HPLC

I was told fluorimetric assay was done previously (Not as accurate as HPLC)

This was done In order to do HPLC :

- A pure sample was purchased from Sigma Aldrich

- Sigma Cat. no. S0947000 - somatropin reference standard

- Link:
- http://www.sigmaaldrich.com/catalog/product/sial/s0947000?lang=en&region=GB (Somatropin European Pharmacopoeia (EP) Reference Standard | Sigma-Aldrich)

- Determine quantity present in grams via HPLC-MS response of intact protein relative to calibration curve of Sigma somatropin standard Cat. no. S0947000 (C1053). Includes determination of intact mass of provided sample.

- HPLC-MS of known quantity Sigma Cat. no. S0947000 - somatropin reference standard to generate 5-point calibration curve

Just sharing some of my testing info

Not sure how other testing is done using 'Humatrope' as a reference standard

Or how AAS samples are tested (what standard is being used)

I have used a Toxicology Laboratory (LC-MS/MS) - AAS Samples

I don't think the testing is done the same (pure sample - external standard)

 

[janoshik]

Where is the pharma hgh vial (serostim) Immunoassay/hplc? On pm?

I can't read pm. I have less ten 50posts.

yes sir

Buck,

I'm on the same page as you, just trying to learn more about the testing methods, process, etc

When I had some GH samples tested in the past, I had asked for "amount present" in each sample vial

After questioning the results (amount seemed off to me) the lab suggested HPLC

I was told fluorimetric assay was done previously (Not as accurate as HPLC)

This was done In order to do HPLC :

- A pure sample was purchased from Sigma Aldrich

- Sigma Cat. no. S0947000 - somatropin reference standard

- Link:
- http://www.sigmaaldrich.com/catalog/product/sial/s0947000?lang=en&region=GB (Somatropin European Pharmacopoeia (EP) Reference Standard | Sigma-Aldrich)

- Determine quantity present in grams via HPLC-MS response of intact protein relative to calibration curve of Sigma somatropin standard Cat. no. S0947000 (C1053). Includes determination of intact mass of provided sample.

- HPLC-MS of known quantity Sigma Cat. no. S0947000 - somatropin reference standard to generate 5-point calibration curve

Just sharing some of my testing info

Not sure how other testing is done using 'Humatrope' as a reference standard

Or how AAS samples are tested (what standard is being used)

I have used a Toxicology Laboratory (LC-MS/MS) - AAS Samples

I don't think the testing is done the same (pure sample - external standard)

That's the correct way to do it.

Using pharma 'standards' leads to less accurate results, but is much cheaper.

 

[G.I. Bro]

Jim I don't exactly understand what the downside of posting a pharma / control sample under the exact some testing methodology as the others will hurt. It will either vindicate the results or show there may be some issue. If you're right and it's no necessary, won't it also just shut everyone up when the results are spot on? What confounds me is why you would resist that if your goal is just to help educate and inform the community.

I'm saying this in a totally non argumentative way. If the control sample is perfect, wouldn't that help end the debate? Even if you believe that's redundant and futile, wouldn't it be worth it in this scenario? Assuming of course it was tested with the exact same procedure and methodology as the others.

If those doesn't make sense, then please enlighten me. I'm being genuine here.

 

[mands]

Here we go round and round in circles again. I have explained, probably 10 times why I think the data is slightly off. You can either trust my experience and reputation in the community and believe what I say or not. But there is no point in continuing to waste time on this stupid argument. The fact remains, once a pharm sample of known concentration is tested then it will either prove me right or it will prove me wrong. Why so many samples have been tested without a control such as this, I have no idea. I would think the responsible thing would be to have a control posted in the first round to ensure accuracy. From someone who preaches science; this methodology that lacks a control group has no scientific basis.

Are we trusting you or are we trusting a GH manufacture that is selling their product on the black market and telling the re-seller is dosed properly? There is a little more to it big guy you know that.

I have a 4mg Serostim that has been sent in for testing as well.

Testing is on HOLD until I can get some information from JIM. Then the testing will resume for all samples already sent in.

mands

 

[Mr MASSE]

We all hope that it won't be to long on hold...
It's so amazing to see test results...
But sure they should be as accurate as possible..

[emoji17]

 

[muscle96ss]

Are we trusting you or are we trusting a GH manufacture that is selling their product on the black market and telling the re-seller is dosed properly? There is a little more to it big guy you know that.

I have a 4mg Serostim that has been sent in for testing as well.

Testing is on HOLD until I can get some information from JIM. Then the testing will resume for all samples already sent in.

mands

You should be trusting/analyzing a number of things; your knowledge of the product and where it cone from, my knowledge and experience, the reputation of a seller who has always supported testing of his products and has recalled a batch when there was a legitimate question on dosage, various other lab tests thst have been reported, and numerous serum and igf-1s that have been performed all over the community. The evidence needs to be looked at in whole where one needs to analyze and then say does this make logical sense or are there too many things pointing in a diffrrent direction that it doesnt make sense and maybe we shouldnt draw any conclusions until we have more information.

 

[CensoredBoardsSuck]

Re ProMuscle's "elusive" GH standard:

The standard was not posted and most of the data from 6 month old testing is not available anymore,

 

[Eman]

Re ProMuscle's "elusive" GH standard:

I've just been skimming a lot of the posts in this thread as of late... I missed that though.

Calling it "6 month old" like that is a long duration in any sense of the word is ludicrous. Says a lot about the testing IMO. Any legitimate company, or illegitimate for that matter I'd imagine, would keep records for several years at a minimum.

 

[janoshik]

I've just been skimming a lot of the posts in this thread as of late... I missed that though.

Calling it "6 month old" like that is a long duration in any sense of the word is ludicrous. Says a lot about the testing IMO. Any legitimate company, or illegitimate for that matter I'd imagine, would keep records for several years at a minimum.

I purge data in a regular manner for my protection, except the most important, which are backed up.

That is much more imporant to me than what people think of that.

Also, I am not sure what 6 months is to you, but for me it's hundreds of samples tested along with doing my 'normal' job, which is no less than 8 hours daily (weekends as well).
Count postgrad education into that and you might notice why 6 months seem like ages to me.

 

[mands]

You should be trusting/analyzing a number of things; your knowledge of the product and where it cone from, my knowledge and experience, the reputation of a seller who has always supported testing of his products and has recalled a batch when there was a legitimate question on dosage, various other lab tests thst have been reported, and numerous serum and igf-1s that have been performed all over the community. The evidence needs to be looked at in whole where one needs to analyze and then say does this make logical sense or are there too many things pointing in a diffrrent direction that it doesnt make sense and maybe we shouldnt draw any conclusions until we have more information.

I totally agree.

You're earlier post you were talking to Jim and said trust you or not. Just too many other factors involved I think even if we have all the information(or we think we do) to completely trust it's all accurate information.

I wasn't trying to discredit you at all. I was just stating the fact that I have as much inside information as you do about GH but, I still don't trust some Chinese factory or lab technician that's selling GH on the black market and supplying me with a lab analysis. I think we all would be naive to do so.

Here's a question, If we come out and post that the Serostim 4mg sample is overdosed? What do you think that would mean? Considering you think all samples tested have 25% more in the vials than what the AAA testing is stating.

mands

 

[mands]

Here's another question. What do we do now? He have a grey top sample sent in and it's right on the money. Well it's .02mg off!!!

mands

 

[rpbb]

Here's another question. What do we do now? He have a grey top sample sent in and it's right on the money. Well it's .02mg off!!!

mands

This should be entertaining

 

[Eman]

Well it's .02mg off!!!

Underdosed!!!

I purge data in a regular manner for my protection, except the most important, which are backed up.

That is much more imporant to me than what people think of that.

Also, I am not sure what 6 months is to you, but for me it's hundreds of samples tested along with doing my 'normal' job, which is no less than 8 hours daily (weekends as well).
Count postgrad education into that and you might notice why 6 months seem like ages to me.

Fair enough however, it's pretty convenient wouldn't you say? Or inconvenient perhaps.

6 months to me is... 6 months.

I suppose you're doing illegal things, not sure exactly what the laws are like where you're at but using the excuse it was a whole 6 months ago isn't much of an excuse... It's a sketchy excuse. In my world, I've generated thousands of files in a 6 month time span and I can go back years to produce copies. That's why I think it's odd you don't have one from 6 months ago...

 

[mands]

Underdosed!!!





Fair enough however, it's pretty convenient wouldn't you say? Or inconvenient perhaps.

6 months to me is... 6 months.

I suppose you're doing illegal things, not sure exactly what the laws are like where you're at but using the excuse it was a whole 6 months ago isn't much of an excuse... It's a sketchy excuse. In my world, I've generated thousands of files in a 6 month time span and I can go back years to produce copies. That's why I think it's odd you don't have one from 6 months ago...

I believe at first anything within 180 days needed a warrant and anything after 180 days you did not need one. I believe a law has past now that you need a warrant for all emails no matter how old/time span. That's in the US. I don't know the laws over seas.

You have less protection if you use Draft emails as communication. So don't think you are being sneaky

mands

 

[ProfessorX]

Here's another question. What do we do now? He have a grey top sample sent in and it's right on the money. Well it's .02mg off!!!

mands

Mands

What does this mean exactly on the report:

* Could be a contaminant that co-eultes with MET but if so, it coelutes exactly.


rhGH (somatropin) and its N-methionyl variant (Met-hGH) (Somatrem) ?

 

[mands]

It's been discussed in earlier posts. Most of the generics have high amounts of glycine in them so it's not unusual.

I will go past an re-post. Give me a few though.

mands

 

[Dr JIM]

Dr. Jim
I will go at this from another angle,
we have seen several, and based on the results posted, I wont even call them generic but rather bio similar HGH samples compared to the standard.
Will we see How a Pharma sample compares to the standard?
How close will the Pharma grade sample have to be to the standard to still be allow for use and sale as HGH by the FDA?
What would you xpect would b acceptable?

also I tried the science xchange route several times. no luck thus far.
this is there response to me from a few days ago......


Dear Buck,
My name is JR and I am a Customer Success Manager at Science Exchange. I am writing to follow-up on your request forAmino Acid Analysisservice that you've submitted to one of our service providers,Proteomics and Mass Spectrometry Facility.
Science Exchange is an online marketplace where scientists can outsource research services to our network of expert service providers. These research services typically encompasses scientific research in the pre-clinical and clinical pipeline.
The request that you have submitted presents several legal complications and Science Exchange is unable to support your project at this time. We cannot support and facilitate requests for services that are intended for personal use, therefore your request for quotation has been deleted from our platform.
If you would like to pursue this request, please provide me with the following information and our team can reassess the request:

1. Please provide me with your work affiliated email (not a Hotmail, Gmail, or Yahoo email address).
2. What is the intended use of the data or product? If it will be used for basic scientific research, please provide me with references to your past publications.

Please let me know if you have any questions.

Best regards,

JR

Google; Amino Acid Analysis Compare Price List

The TOP listing on the GOOGLE search page will be the "Science Exhange"

CLICK ON IT!

The follow up Science Exhange page has listed 18 AA analytical vendors that YOU can contact directly.

I noticed you submitted a "request for research". Understand no U.S. analytical lab that I'm aware of will conduct an QQ assay on controlled substances UNLESS BOTH YOU and THEY are registered with the DEA to conduct such research.

However since GH is NOT a controlled substance a DEA registration is neither required
by yourself or the lab. If you are told anything to the contrary, its a "labs policy" issue rather than a requirement mandated by the federal government.

Sorry about the confusion
JIM

 

[Dr JIM]

^^^^^

Also do NOT tell the involved lab the assays are being conducted for "personal use" bc that's the gray area I spoke of some time ago as the "results" may be misinterpreted as permission to USE an illicit drug!

That's one reason why Ive never opined how these results may be used on an individual basis.

 

[rpbb]

Here's another question. What do we do now? He have a grey top sample sent in and it's right on the money. Well it's .02mg off!!!

mands

No comments from all the people trying to discredit the testing?

 

[mands]

Here's another question. What do we do now? He have a grey top sample sent in and it's right on the money. Well it's .02mg off!!!

mands

Dammit it's .05mg sorry

mands