THE new "Generic" HGH Assay PAGE! AAA testing

[Sdryx]

I only brought it up once. Not creating clutter here. Just pointing out an observation and responding to replies to myself.

I agree with you to drop it. I've made my point.

 

[rpbb]

The chart has been posted here many times and the Serostim results are on the chart. As for the data behind the results, muscle has offered to send it to you. Not sure why he doesn't just send it to mands.

You want it posted publicly, but as far as I know, posting PM's data publicly would result in a ban from PM. Silly, perhaps, but he does post a lot on that board and so does buck so I wouldn't imagine they would risk that.

 

[Just Fish]

Lol

 

[BigAli]

Laughed so much at this. You made my day "rpbb".
These people are MAD or no it sounds more like a cry baby..OUR SITE
I'm waiting for heady muscle infamous post "stop the banter " lol

 

[Dr JIM]

That certainly is a wonderful and factual answer to my question, like always, Mr. JIM.

Reference standards can be acquired thru the USP, and via a variety of commercial analytical sites. Such standard may also be available thru the European Pharmocopeia.

It's also perfectly acceptable to develop your own GH standard using an AAS as the means of quantification JUST LIKE THE WHO has done.

Of course the latter requires one to be
AAA proficient, and that I'm told requires several years of AAA experience (depending upon volume) before meeting the criteria necessary for "research certification" in the U.S.

The lab we are using has compiled "close to 15 years" of AAA data to use for comparison when outliers occur, and rest assured no unusual (or more importantly, unexplained) outliers have been detected to date with respect to these assays!

 

[rpbb]

Reference standards can be acquired thru the USP, and via a variety of commercial analytical sites.

It's also perfectly acceptable to develop your own GH standard using an AAS as the means of quantification JUST LIKE THE WHO has done.

Of course the latter requires one to be
AAA proficient, and that I'm told requires several years of AAA experience (depending upon volume) before meeting the criteria necessary for "research certification" in the U.S.

The lab we are using has compiled "close to 15 years" of AAA data to use for comparison when outliers occur, and rest assured no unusual (or more importantly, unexplained) outliers have been detected to date with respect to these assays!

or you do what janos did, by an hplc machine on craigslist, and buy a bunch ugl hgh and gear from his bro's at the gym who said it was gtg, use that as the standards, and than start charging noobs and dimwits for testing

 

[Dr JIM]

Standards are like many other "drugs" most are controlled and are distributed on a "needs basis"

That's to say they will likely be difficult, for anyone who is not
registered in some way fashion or form, to acquire them.

So yea if you're wanting info on how to obtain such standards as an uncertified lab, I have NO IDEA.

Which brings me to; I understand you're also conducting AAS assays?

Well once again I've no idea how that is possible wo the required standards.

However if you think a GH standard is difficult to obtain, the acquisition of controlled substance standards is a NIGHT MARE in comparison.

That's bc exclusive of TT, any U.S. lab even possesses anabolic agents is required to be a DEA registered and certified research lab!

Funny how I have close to 20 AAS standards and if you did not have to be such a condescending prick from day one, I would have GIVEN them to you, with the proviso they be used to properly conduct and release the data obtained.

 

[heady muscle]

o much at this. You made my day "rpbb".
These people are MAD or no it sounds more like a cry baby..OUR SITE
I'm waiting for heady muscle infamous post "stop the banter " lol[/QUOTE]
Stay out this rookie.

 

[BigAli]

o much at this. You made my day "rpbb".
These people are MAD or no it sounds more like a cry baby..OUR SITE
I'm waiting for heady muscle infamous post "stop the banter " lol

Stay out this rookie. [/QUOTE]

Ok Mommy

 

[Dr JIM]

Being "banned" from PM, a prerequisite of Meso membership as it proves the ostracized member is capable of deductive reasoning.

 

[CensoredBoardsSuck]

Re amino-acid analysis [AAA]:


An NIH study citing an AAA for GH quantification AND HPLC as a secondary assay for the detection of PEP byproducts.

[The Somatropin Reference Standard (Control 951) of the National Institute of Health Sciences].
Yomota C, et al. Eisei Shikenjo Hokoku. 1996.
Show full citation
Abstract
Somatropin material was examined for preparation of the "Somatropin Reference Standard". The candidate material was evaluated by a domestic collaborative study in which eight laboratories participated. The protein content was determined to be 4.5 mg/Vial based on amino acid analysis. Because of the possibility of application as a chemical reference standard for assay by the HPLC method, a physico-chemical evaluation of the candidate material was also performed. By SE-HPLC, the content of polymer, dimer were determined to be 0.54%, 0.98%, respectively. By RP-HPLC, the early peak area ascribed to desamido and sulfoxide form was 1.07% of the total peak area. And for informational data, the potency of the candidate material, being estimated by three different biological methods, weight gain assay, tibia test and adiposeconversion assay is 14.8 IU/vial. Based on the above results, the candidate was authorized as the Somatropin Reference Standard of the National Institute of Health Sciences.



Note the standard used to develop the WHO GH criteria was an AAA.

The First International Standard for Somatropin: report of an international collaborative study.
Bristow AF, et al. Growth Regul. 1995.
Show full citation
Abstract
Following an earlier decision to move away from the in vivo bioassay for determination of the potency of therapeutic somatropin (recombinant DNA human growth hormone), 18 laboratories in 12 countries participated in an international collaborative study designed to establish an international standard for somatropin, calibrated both by bioassay and by physicochemical assays of somatropin content. The mean in vivo biological potency of preparation studied, coded 88/624, was 6.75 IU/ampoule (fiducial limits 6.30-7.23). Determination of the protein content by quantitative amino-acid analysis yielded a mean estimate of 1.98 mg protein per ampoule. (Relative standard deviation = 2.88%). Data from the study also yielded mean values of 97.2% +/- 0.8% for the monomer content of the preparation, and 8.18 (RSD = 4.00%) for A1% at 276 nm. At its 45th meeting, in October 1994, the ECBS of WHO formally established the preparation 88/624 as the First International Standard for Somatropin, with a defined content of 2.0 mg protein per ampoule, and a defined specific activity of 3.0 International Units per milligram.



This study assigned the currently used mg to IU ratio for rHGH.

Although SE/HPLC was the assay
used, quantification once again relied on the FIRST IRS which was based
on a AAA.

The Second International Standard for somatropin (recombinant DNA-derived human growth hormone): preparation and calibration in an international collaborative study.
Bristow AF, et al. Biologicals. 2001.
Show full citation
Abstract
A preparation of somatropin (recombinant DNA-derived human growth hormone) was prepared as lyophilised ampoules according to WHO procedures for international biological standards. The candidate preparation (98/574) was evaluated in an international collaborative study (16 laboratories, nine countries), with the following aims: (i) to determine the suitability of the preparation to serve as the International Standard for somatropin by studying its performance in the current range of physico-chemical and biological assay methods employed for somatropin; (ii) to assign a content in terms of the existing (first) International Standard for somatropin, using the currently recognised assay procedure (Size Exclusion High Performance Liquid Chromatography, SE HPLC); (iii) to confirm the specific biological activity of the candidate preparation; (iv) to confirm the stability of the candidate preparation. On the basis of the collaborative study WHO agreed that: the preparation in ampoules coded 98/574 is suitable to serve as the next WHO International Standard for somatropin; the preparation in ampoules coded 98/574 should be established as the second International Standard for somatropin, with a defined ampoule content of 1.95 mg total somatropin plus somatropin-related proteins per ampoule; the specific activity of the preparation should be defined as 3.0 IU/mg somatropin.

 

[CensoredBoardsSuck]

o much at this. You made my day "rpbb".
These people are MAD or no it sounds more like a cry baby..OUR SITE
I'm waiting for heady muscle infamous post "stop the banter " lol[ /QUOTE]
Stay out this rookie.

How does an experienced Meso member mangle a quotation this badly and then have the audacity to call someone else a rookie? It boggles the mind.

 

[Eman]

I saw that, CBS... Edit is a godsend

 

[rpbb]

Re amino-acid analysis [AAA]:


An NIH study citing an AAA for GH quantification AND HPLC as a secondary assay for the detection of PEP byproducts.

[The Somatropin Reference Standard (Control 951) of the National Institute of Health Sciences].
Yomota C, et al. Eisei Shikenjo Hokoku. 1996.
Show full citation
Abstract
Somatropin material was examined for preparation of the "Somatropin Reference Standard". The candidate material was evaluated by a domestic collaborative study in which eight laboratories participated. The protein content was determined to be 4.5 mg/Vial based on amino acid analysis. Because of the possibility of application as a chemical reference standard for assay by the HPLC method, a physico-chemical evaluation of the candidate material was also performed. By SE-HPLC, the content of polymer, dimer were determined to be 0.54%, 0.98%, respectively. By RP-HPLC, the early peak area ascribed to desamido and sulfoxide form was 1.07% of the total peak area. And for informational data, the potency of the candidate material, being estimated by three different biological methods, weight gain assay, tibia test and adiposeconversion assay is 14.8 IU/vial. Based on the above results, the candidate was authorized as the Somatropin Reference Standard of the National Institute of Health Sciences.



Note the standard used to develop the WHO GH criteria was an AAA.

The First International Standard for Somatropin: report of an international collaborative study.
Bristow AF, et al. Growth Regul. 1995.
Show full citation
Abstract
Following an earlier decision to move away from the in vivo bioassay for determination of the potency of therapeutic somatropin (recombinant DNA human growth hormone), 18 laboratories in 12 countries participated in an international collaborative study designed to establish an international standard for somatropin, calibrated both by bioassay and by physicochemical assays of somatropin content. The mean in vivo biological potency of preparation studied, coded 88/624, was 6.75 IU/ampoule (fiducial limits 6.30-7.23). Determination of the protein content by quantitative amino-acid analysis yielded a mean estimate of 1.98 mg protein per ampoule. (Relative standard deviation = 2.88%). Data from the study also yielded mean values of 97.2% +/- 0.8% for the monomer content of the preparation, and 8.18 (RSD = 4.00%) for A1% at 276 nm. At its 45th meeting, in October 1994, the ECBS of WHO formally established the preparation 88/624 as the First International Standard for Somatropin, with a defined content of 2.0 mg protein per ampoule, and a defined specific activity of 3.0 International Units per milligram.



This study assigned the currently used mg to IU ratio for rHGH.

Although SE/HPLC was the assay
used, quantification once again relied on the FIRST IRS which was based
on a AAA.

The Second International Standard for somatropin (recombinant DNA-derived human growth hormone): preparation and calibration in an international collaborative study.
Bristow AF, et al. Biologicals. 2001.
Show full citation
Abstract
A preparation of somatropin (recombinant DNA-derived human growth hormone) was prepared as lyophilised ampoules according to WHO procedures for international biological standards. The candidate preparation (98/574) was evaluated in an international collaborative study (16 laboratories, nine countries), with the following aims: (i) to determine the suitability of the preparation to serve as the International Standard for somatropin by studying its performance in the current range of physico-chemical and biological assay methods employed for somatropin; (ii) to assign a content in terms of the existing (first) International Standard for somatropin, using the currently recognised assay procedure (Size Exclusion High Performance Liquid Chromatography, SE HPLC); (iii) to confirm the specific biological activity of the candidate preparation; (iv) to confirm the stability of the candidate preparation. On the basis of the collaborative study WHO agreed that: the preparation in ampoules coded 98/574 is suitable to serve as the next WHO International Standard for somatropin; the preparation in ampoules coded 98/574 should be established as the second International Standard for somatropin, with a defined ampoule content of 1.95 mg total somatropin plus somatropin-related proteins per ampoule; the specific activity of the preparation should be defined as 3.0 IU/mg somatropin.

AND that answers that question. I wonder if Janos will say his testing is better than the NIH

 

[heady muscle]

MESO is a hilarious forum. Instead of informing young guys who have no idea what they're doing with gear you guys are biting each other all the time...WOW!!! and then if OP asks a question in 1 day has to go through at least 10 pages of pure B.S. !!

You're like a weather vane, whatever way the wind blows you go. lol You are a fraud.

 

[Dr JIM]

AND that answers that question. I wonder if Janos will say his testing is better than the NIH

The importance of these studies can not be overemphasized, to suffice it to say although rHGH was approved for use decades ago, qualitative and quantitative uniformity was lacking on a world wide basis.

Consequently one of the WHO objectives was to expedite the development of a GLOBAL STANDARD that would serve as the QUALITATIVE and QUANTITATIVE baseline from which ALL other manufactured rHGH would be judged.

So of course the existing USP and EP standards are using the WHO criteria for rHGH standard compliance, bc nonconformity would likely result in
the revocation of any production facilities license to manufacture HGH.

 

[rpbb]

The importance of these studies can not be overemphasized, to suffice it to say although rHGH was approved for use decades ago, qualitative and quantitative uniformity was lacking on a world wide basis.

Consequently one of the WHO objectives was to expedite the development of a GLOBAL STANDARD that would serve as the QUALITATIVE and QUANTITATIVE baseline from which ALL other manufactured rHGH would be judged.

So of course the existing USP and EP standards are using the WHO criteria for rHGH standard compliance bc their HGH would otherwise NOT be approved as Pharma.

Muscle and Janos are going going have to explain why the greys are underdosed

 

[BigAli]

You're like a weather vane, whatever way the wind blows you go. lol You are a fraud.

OH Haha bingo for you!! so you got so much time to go through my past posts..well done!! Bc I'm very busy right now buying my next 100K car which is gorgeous btw

The reason for that post was only bc I wanted to get some info out of thread and not going through crap. And bc I like MESO since it's not a sponsor board...Compare this to PM!

FRAUD!! How then?
Dickhead y muscle..I'm not selling anything and not buying anything either! I'm chilling..I've posted my gears' pic in another thread delivered to me from Pharmacy!! So wealthy don't even need to bother with UGLs..

All my intention is to learn more about PEDs and inform members who are unaware of health issues that they might undergo for abusing not standardized UGL shit!! Fortunately I don't make money playing with people's health and God Bless whoever helps a bro for a healthier life not someone like you watching who posts what...We got a new copper here! And I don't really have to do anything with you dumbass if you stop watching people.

 

[Eman]

OH Haha bingo for you!! so you got so much time to go through my past posts..well done!! Bc I'm very busy right now buying my next 100K car which is gorgeous btw

The reason for that post was only bc I wanted to get some info out of thread and not going through crap. And bc I like MESO since it's not a sponsor board...Compare this to PM!

FRAUD!! How then?
Dickhead y muscle..I'm not selling anything and not buying anything either! I'm chilling..I've posted my gears' pic in another thread delivered to me from Pharmacy!! So wealthy don't even need to bother with UGLs..

All my intention is to learn more about PEDs and inform members who are unaware of health issues that they might undergo for abusing not standardized UGL shit!! Fortunately I don't make money playing with people's health and God Bless whoever helps a bro for a healthier life not someone like you watching who posts what...We got a new copper here! And I don't really have to do anything with you dumbass if you stop watching people.

I've known a lot of wealthy people in my life... They all had one thing in common. They didn't brag about their wealth on an Internet forum. So needless to say, I believe everything you say Ali.

 

[BigAli]

I've known a lot of wealthy people in my life... They all had one thing in common. They didn't brag about their wealth on an Internet forum. So needless to say, I believe everything you say Ali.

I didn't really brag about it; I was telling the guy go waste your time somewhere else implicitly by mentioning my progress! but I know many people (friends,family) talking about their wealth wo anyone calling it bragging. We call it motivation!

And I don't really need anyone to believe anything I say

You can go through my posts with JIM (which I believe is a respectable individual in Meso and by most of you) when he asked me some questions and in response he told me at least I'm HONEST. Not looking for anything really here other than some info to raise the quality of my life and health. Have learnt so much so far by @Dr JIM and other member's guides