“Legit” raws are far from difficult to find
MESO-Rx
Anabolic Steroids
Why Home Brew?
- Thread starter NorthMich
- Start date
This is such an excellent forum. Excellent advice, data-driven, tons of experience and anecdotes and minimal bickering
Amazing
Amazing
@picholas mind sharing what kind of autoclave you purchased? All the ones I've looked into are in the range of 800-1500
Thanks
Arcânn
Member
To the best of my understanding, it's actually easier to find legit raws than it is to find legit finished product?
RCCP
Member
You can also look up heat sterilizers same stuffs used in nail salons and dental offices. They get up to 200c that should be enough to sterilizing and they cost much less than an aclaves
picholas
New Member
I've posted pictures of it before, I'll PM you.
picholas
New Member
Mine goes over 220°C
No recommendations but amazon has some under $150. Another thing would be a $40 toaster oven, those get up to 4 or 500F.
What’s the difference between an autoclave, heat sanitizer, or an simple toaster oven? If I buy a brand new convection oven and all it’s ever used for is heating vials and caps how is that less sanitary than an autoclave?
RCCP
Member
Another thing is what are you trying to sterilize? if you're simply clanning the empty bottles then a simple steam autoclave would work because there is no oil to interact with the water. In a pressurised steam autoclave the temp needs to be above 130c for 15 minutes. If you are using dry heat oven or autoclave you need to be over 175c for 1 hour. So there is a give or take. In a perfect lab you would have a steam autoclave to sterilize the vials as well as the beakers, rods, etc. as it's much more efficient. The only reason you would NEED to use dry heat is in the presence of powders, oils, or something likely to rust, after that it's just preference.
A good home set up would be a dedicated oven, although one kee difference between ovens and autoclaves would be airflow. An autoclave of any kind is sealed and therefore no airflow to allow additional contaminants in. An oven is open airflow and could allow contaminates in that would technically reset the 1 hour+ requirement. However if you have a clean space with limited outside airflow then you should be fine.
A good home set up would be a dedicated oven, although one kee difference between ovens and autoclaves would be airflow. An autoclave of any kind is sealed and therefore no airflow to allow additional contaminants in. An oven is open airflow and could allow contaminates in that would technically reset the 1 hour+ requirement. However if you have a clean space with limited outside airflow then you should be fine.
picholas
New Member
I do not doubt one second that a toaster oven is not capable to sterilize the instruments. But very few of us measure the temperature with a reliable thermometer and even fewer will look for cold spots. If ever one of us tried to make a cake in a toaster oven or normal oven, one realizes what I am talking about. The WHO says this about it:
Temperature
(°C)
Minimum sterilization time
(min)
160°C
180 min
170°C
60 min
180°C
30 min
Specific conditions of temperature and time for certain preparations are stated in individual monographs.
The oven should normally be equipped with a forced air system to ensure even distribution of heat throughout all the materials processed. This should be controlled by monitoring the temperature. Containers that have been temporarily closed during the sterilization procedure are sealed after sterilization using aseptic techniques to prevent microbial recontamination.
The bioindicator strain proposed for validation of the sterilization process is: spores of Bacillus subtilis (e.g. var. niger ATCC 9372 or CIP 77.18) for which the D-value is 5-10 minutes at 160 °C using about 10 EXP 6 spores per indicator.
A toaster oven has a thermostat, but what are the ranges of temperature control? How easy is it to change the temperature? How long will it last and will it give an error code if the sterilization has not been successful?
Great post ^^^^
What’s a good size to look for?
What’s a good size to look for?
picholas
New Member
Really depends what you intend to use it for (vials only can be smaller). I am not home to check right now, but I think mine is
H: 14.5 in
W: 19.0 in
H: 14.5 in
W: 19.0 in
