Dirts Brew log

[AnTabolic73]

isn't the cap coming with the media bottle filter set of ks-tek already sterilized?
my issue is if when I open the media bottle to suck the oil out with the syringe during that time it can create some sterility issue.

Those bottle top/media bottle combos tend to crack if you use too much pressure. Thats why most opt for glass media bottles. But then you have to go down the sterilization rabbit hole.

I brew my test in 200ml batches sometimes. And tho it sucks, I use syringe filters into sealed sterile vials.

I use 20ml syringes and do it by hand. The larger syringes( 60ml) are ridiculously hard to depress without a caulking gun. Even with it. The pressure is sometimes too much for the syringes and they can warp and push though the caulking gun. So I've just done it by hand, it's easier.

 

[AnTabolic73]

@Dirthand

From your experience with tne. Can you suggest a recipe that wouldn't require guaiacol. I'd be willing to use ethyl oleate/mct/ba/bb and keep the concentration to 50mg/mL

 

[Dirthand]

Those bottle top/media bottle combos tend to crack if you use too much pressure. Thats why most opt for glass media bottles. But then you have to go down the sterilization rabbit hole.

I brew my test in 200ml batches sometimes. And tho it sucks, I use syringe filters into sealed sterile vials.

I use 20ml syringes and do it by hand. The larger syringes( 60ml) are ridiculously hard to depress without a caulking gun. Even with it. The pressure is sometimes too much for the syringes and they can warp and push though the caulking gun. So I've just done it by hand, it's easier.

I agree I have had one of the bottles crack before.. I did try the 20ml syringes by hand but I prefer caulking gun.. I found a very large size gun to where I can use 100ml syringes and just change the filter out in the middle.. sometimes I do do both.. say I do not feel like vialing up 25 vials I will bottletop filters into a glass media bottle with a silicone septa and draw out and filter again into vials as needed .. I have have sterility test performed on this method and it works..

 

[AnTabolic73]

I agree I have had one of the bottles crack before.. I did try the 20ml syringes by hand but I prefer caulking gun.. I found a very large size gun to where I can use 100ml syringes and just change the filter out in the middle.. sometimes I do do both.. say I do not feel like vialing up 25 vials I will bottletop filters into a glass media bottle with a silicone septa and draw out and filter again into vials as needed .. I have have sterility test performed on this method and it works..

Mind posting or linking to your caulking gun?

I have a cheap one from home depot. Maybe I'll try a new one out.

 

[Dirthand]

@Dirthand

From your experience with tne. Can you suggest a recipe that wouldn't require guaiacol. I'd be willing to use ethyl oleate/mct/ba/bb and keep the concentration to 50mg/mL

At 50mg/ml you may not even have to use EO just a higher amount of bb.. I have never er tried at that concentration but if I was I'd do something like this and see.
30% EO
25% BB
1.5%ba
Mct who knows if it would hold.. it is just where I'd start.. I have done probly 12 different ways to make tne.. so I deffo know what do t work for sure!! Lol

my full proof 100mg/ml TNE
25/75 EO/MCT
20%bb
18%guaiacol
1.2%ba
I can do a whole ml of that no pip... I realize EO and guaiacol is not what most people want to hear. .
Some people are doing 50mg/ml in castor oil with 45% BB to hold and thin.. I did a castor oil a little higher mg but I pinned it and that shit hurt me like hell.. I gave some to a guy and he lives it.. so I don't know if it was the castor oil or that high of bb that doesn't agree with me.. high%EO does not agree with me either.

 

[Dirthand]

Mind posting or linking to your caulking gun?

I have a cheap one from home depot. Maybe I'll try a new one out.

Yes I'm at work but when I get home I sure will!!!

 

[AnTabolic73]

At 50mg/ml you may not even have to use EO just a higher amount of bb.. I have never er tried at that concentration but if I was I'd do something like this and see.
30% EO
25% BB
1.5%ba
Mct who knows if it would hold.. it is just where I'd start.. I have done probly 12 different ways to make tne.. so I deffo know what do t work for sure!! Lol

my full proof 100mg/ml TNE
25/75 EO/MCT
20%bb
18%guaiacol
1.2%ba
I can do a whole ml of that no pip... I realize EO and guaiacol is not what most people want to hear. .
Some people are doing 50mg/ml in castor oil with 45% BB to hold and thin.. I did a castor oil a little higher mg but I pinned it and that shit hurt me like hell.. I gave some to a guy and he lives it.. so I don't know if it was the castor oil or that high of bb that doesn't agree with me.. high%EO does not agree with me either.

I've brewed some dhb using guaiacol and i got knots and flu like symptoms. Threw out the whole batch.

I'd be willing to try eo. Make a small batch and see how it feels. Gonna do the same with mct. Haven't tried it mainly because gso works fine for me. But this old dog gotta learn some new tricks.

But I'll use your recipe and adjust from there. Thanks.

Well once qsc comes back from vacation. TNE is on my list.

 

[Dirthand]

I've brewed some dhb using guaiacol and i got knots and flu like symptoms. Threw out the whole batch.

I'd be willing to try eo. Make a small batch and see how it feels. Gonna do the same with mct. Haven't tried it mainly because gso works fine for me. But this old dog gotta learn some new tricks.

But I'll use your recipe and adjust from there. Thanks.

Well once qsc comes back from vacation. TNE is on my list.

I love me some tne man!! I am going to try for a tne/trenbase preworkout blend before long.. I log the experience here in the log so if anyone had thought?

 

[Sampei]

I still see a lot of debate on EO, in Europe it is approved to be used in progesterone injection so it shouldn't be that bad... At the same time how many progesterone injection one do? Not the same volume we do... And the FDA never approved EO for injection.

Do we have other studies on it and it's detrimental effects? (Systemic inflammation etc)

@narta