Dr Jims Hplc/ms Data

[BigAngus]

You just dont take this as seriously as I do Angus and that's a shame bc your methods are misleading our members into believing they can rely on your test results to "learn" which labs to buy from and which ones to avoid.

1) The ionic gradient used in HPLC is developed bc of differences in buffering between the mobile vs stationary phases. This generates a hydrophilic vs a hydrophobic gradient which creates difference a substances elution. So it's not some setting that alters a compounds retention but the use of different buffering solvents, that must be calibrated and concocted by the analyst. So there are some exceptions, such as certain sugars, in which the column does make a difference but ASS all use a relatively nonspecific hydrophobic/philic column.

2) No we are not building rockets ANGUS, but when your tests reveal an Oxandrolene "purity" of 100% yet the same sample I had tested (using a calibration curve and THREE HPLC runs utilizing THREE different UV wave lengths) failed to reveal the presence of ANY ANAVAR, it should become obvious to all this is NOT ABOUT ROCKETS at all!

No Angus it's about being forthright and honest regarding the inadequacies and severe limitations of "your lab" most of which fail to meet ANY existing analytical lab standards.

3) But you consider "that a functional", Oxandrolone result, who are you kidding!!

Sorry ANGUS but IMO there is a HUGE problem when someone claims to be providing a service that is NOT capable of producing reliable and reproducible results.

I love reading your opinions. Please continue. You spin an awesome tale, love it.

 

[flenser]

@BigAngus Can you answer an interpretation question on the results of your tests? I'm not sure if the concentration listed is the concentration of the powder, or of the intended aas.

For example, a test p result recently posted had a purity of 29% and concentration of 76 mg/ml. Does that mean the UGL uses 76 mg/ml raw powder or 2.6 times that?

This has been the source of some very long arguments here, and I don't think we have reached a consensus.

 

[Dr JIM]

I love reading your opinions. Please continue. You spin an awesome tale, love it.

A tale that's true however!

 

[johnnyBALLZ]

Damn JB wasn't that the lab in which several fellas from other forums were OVERTLY SUPPORTIVE of their quality?

So much so that they openly challenged you to produce "proof" that their products were ANYTHING BUT GTG?

I could be wrong and have the threads confused, but let Meso members know for sure.
(I also need to know for another reason.)

Regs
JIM

Yes, Karius/Alpha was the lab IM went into a ridiculous 2 month war over..

 

[Dr JIM]

Wasn't sure if it was them.

I was hoping I would be able to use HPLC almost exclusively for the analyses, in part bc of the added expense.

However with such as overt support exclusive from Meso and an outright challenge for "proof", I suspect in this instance there should be essentially no doubt.

Ergo I'll have a MS conducted on your sample, PROVIDING YOU DON'T MIND JB!

Heck, perhaps it will be even more beneficial from a teaching perspective.

jim

 

[brutus79]

@Dr JIM
Regular asked -

thanks

Does peak five on JB-2 indicate that 86.8 % of the tablet is tren?

 

[CensoredBoardsSuck]

@Dr JIM
Regular asked -

I believe AS1646-8 is your sample. If that's the case, the purity is 0.2% trenbolone.

 

[Dr JIM]

@Dr JIM
Regular asked -

What sample number are you referencing Reg?
Is it 46-7?

 

[brutus79]

What is the sample number your referring to Reg?

It was file jb 2

 

[Dr JIM]

Ok give me a sec to check it out but I specifically recall mentioning someone may CLAIM this was the concentration in the narrative, and I can tell you the answer is a resounding NO!

"I'll be back"!

 

[Dr JIM]

Brutus thats not the sample number BUT I believe what your referred to is the "JB" SAMPLE (....146-8) written on the graph pages, correct?

 

[brutus79]

AS1646-8

 

[Dr JIM]

Well if so yes that is Tren which was detected in a VAR pill that JB submitted. But It's only reflective of the PEAK area percentages, rather than a specific concentration as in mg/ml.

The SUMMARY of this sample used a Trenbolone concentration curve (derived by the chemist using a Research Grade Tren stock solution) to determine the mg contained within the pill, relying upon the AUG percentage, you mentioned.

Got me!

I thought earlier is was another sample where I mentioned the coned in portal.

 

[CensoredBoardsSuck]

Well if so yes that is Tren which was detected in a VAR pill that JB submitted. But It's only reflective of the PEAK area percentages, rather than a specific concentration as in mg/ml.

The SUMMARY of this sample used a Trenbolone concentration curve (derived by the chemist using a Research Grade Tren stock solution) to determine the mg contained within the pill, relying upon the AUG percentage, you mentioned.

Got me!

I thought earlier is was another sample where I mentioned the coned in portal.

So what's the concentration percentage?!! Is it 0.2% as the summary I posted above states?

 

[Dr JIM]

It's a PEAK summation percentage.

Meaning all the peaks combined equal 100%.

Thereafter the AUG or AUC, if one prefers, is determined using an EXACTING computer program which measure the volume of each peak assigning a portion of the whole to each peak.

Thus when all the peaks are added they. should equal 100%.

That percentage is used to calculate the the milligrams in each pill or in this instance 0.2% of each 184mg pill (which was weighed beforehand) was TREN which would approximate 0.4 mg
or 0.36 mg for those who prefer a more exacting figure.

 

[ebkallday]

It's a PEAK stion percentage.

Meaning all the peaks combined equal 100%.

Thereafter the AUG or AUC, if one prefers, is determined using an EXACTING computer program which measure the volume of each peak assigning a portion of the whole to each peak.

Thus when all the peaks are added they. should equal 100%.

That percentage is used to calculate the the milligrams in each pill or in this instance 0.2% of each 184mg pill (which was weighed beforehand) was TREN which would approximate 0.4 mg
or 0.36 mg for those who prefer a more exacting figure.

Could this pill have been methyltren? Or just straight up tren with no way of being absorbed orally?

 

[Dr JIM]

The fact such a large peak can be generated in spite of minuscule sample sizes, tells one just how sensitive and specific a HPLC can be if performed correctly.

These factors alone underscore the fact one can not simply LOOK at the size, or the listed peak percentages to determine a samples concentration in isolation.

Because a calibration curve is MANDATORY to convert AUG percentages into useful purity or concentration data!

SO WHAT IS THR PURITY OF JBs SAMPLE USING THE INFORMATION PROVIDED

(Hint it's pretty damn shitty IMO!)

 

[Dr JIM]

Could this pill have been methyltren? Or just straight up tren with no way of being absorbed orally?

Would it really matter what AAS, designer or otherwise, it contained considering the concentration was 0.4mg PER PILL, hardly!

 

[brutus79]

Why did he use a polar solvent to extract the hormone from the tablet's excipients instead of a non-polar solvent like chloroform, dichloromethane, ether, benzene, toluene, or xylene?

Per the US Pharmacopeia, oxandrolone AKA anavar, is sparingly soluble in polar solvents but freely soluble in non-polar solvents.

http://www.pharmacopeia.cn/v29240/usp29nf24s0_alpha-2-24.html


Per Sigma Aldrich's solubility table, a sparingly soluble substance requires a dilution factor of 1/30 to 1/100 to be dissolved.

http://www.sigmaaldrich.com/united-kingdom/technical-services/solubility.html


Per Dr. Jim's lab report, he used a dilution factor of 1/10.

The report that Dr. Jim presented indicates that 10% to 30% of the amount of solvent that was supposed to be used to dissolve the oxandrolone, was used. That's not even accounting for the fact that extra solvent should have been used to separate the oxandrolone from the excipients.

I'm not sure if oxandrolone is soluble in a 50/50 mix of acetonitrile and water because oxandrolone is "practically insoluble in water" per the US pharmacopeia.

He should have used a significant amount of a non-polar solvent to extract the hormone from the tablet's excipients.

Please order a real test from estacydata and tell them you suspect there is anavar in the tablet.

 

[ebkallday]

Would it really matter what AAS, designer or otherwise, it contained considering the concentration was 0.4mg PER PILL, hardly!

Why yes It would matter. .4mgof methyltren IS a heavy dose. A sstandard dose of methyltren is 250 MCG. So that would put this sample at roughly 400mcg. If this were being passed as anavar, a females favorite steroid, think of what kind of negative effects she would get as a result. So I do think it is important to know if it was methyltren. Just my thoughts.