Genotec Labs

[mp46]

I already know you not gonna like me for what i am about to post but terminal death time of bacteria in forced air Convection oven is 60min at 160c, in eu and usa the same but the sterilisaiton process as we all know is not only about killing bacterias right.

I am not guessing and i am not confused about sterilisation standard hence 120min sterilisation time as per gmp guidelines.

You’re right, I won’t like it. Because, in YOUR link to the CDC they say 120min at 160c. Where are you all of a sudden getting 60min at 160c?

 

[Genotec]

Didn't realize bacteria died faster in countries that used the metric system. I'll keep that in mind.

You shouldn't be guessing if the sterilization times are correct or not depending on who's standard you're using or who's disseminating the information. You should have a definitive concrete answer as to what your sterilization process is and how you came to that conclusion.

You have questiond the sterilisation time given by manufactures saying you did not know bacteris die faster here, so they die in 60min, i am sure you can easly find this info but sterilisation proces is not only about killing the bacteria , so all the bacterias might be dead after 60min but this dosent mean sterilisation process is finished and glass is sterile.

 

[Oldschool]

View attachment 112692

Who is dat?

 

[mp46]

Again. See above post. YOU link to a medical document from the government saying it’s 120min at 160c. What your supplier quoted is irellevant. I’m talking about what YOU said and the sources YOU provided are direct contradictions.

 

[Genotec]

I am not contradicting anything i just corrected you that bacterias in fact die in 60min but endotoxins, spores might take longer so the guidelines recommend 120min which i do.

 

[mp46]

Really dude. Now you’re going to split hairs between sterilizing for bacteria and “endotoxins?” Get. The fuck. Outta here.

How about this; YOUR link says 120min at 160c. YOUR link says that atrophaeus spores should be used to determine whether dry heat sterilization has occurred. YOUR link mentions nothing about endotoxins. YOUR link does mention atrophaeus endospores. Guess what atrophaeus is.... a fuckin bacteria!

Let’s break down some first year biology.

Species: B. atrophaeus
Family: Bacillaceae
Genus: Bacillus
Order: Bacillales

Man... there’s some voodoo shit going on here with all these “bac” words.

Class: Bacilli
Phylum: Firmicutes
Kingdom: Bacteria

What the Fuck is that? Does that say bacteria?


So you’re going to sit here and tell me that bacteria only takes 60 minutes to kill. Yet YOU post information saying atrophaeus is used to determine sterility and YOUR link says that takes 120min at 160c. So either your wrong or your information you linked is wrong. Think I’ll trust the Centers for Disease Control (which YOU linked to) before I trust a drug dealer on the internet.

You may have the best, most sterile gear in the world but until you can articulate that and understand what you’re saying and ge links you provide I hope people stay away from you.

 

[Thaistick]

Really dude. Now you’re going to split hairs between sterilizing for bacteria and “endotoxins?” Get. The fuck. Outta here.

How about this; YOUR link says 120min at 160c. YOUR link says that atrophaeus spores should be used to determine whether dry heat sterilization has occurred. YOUR link mentions nothing about endotoxins. YOUR link does mention atrophaeus endospores. Guess what atrophaeus is.... a fuckin bacteria!

Let’s break down some first year biology.

Species: B. atrophaeus
Family: Bacillaceae
Genus: Bacillus
Order: Bacillales

Man... there’s some voodoo shit going on here with all these “bac” words.

Class: Bacilli
Phylum: Firmicutes
Kingdom: Bacteria

What the Fuck is that? Does that say bacteria?


So you’re going to sit here and tell me that bacteria only takes 60 minutes to kill. Yet YOU post information saying atrophaeus is used to determine sterility and YOUR link says that takes 120min at 160c. So either your wrong or your information you linked is wrong. Think I’ll trust the Centers for Disease Control (which YOU linked to) before I trust a drug dealer on the internet.

You may have the best, most sterile gear in the world but until you can articulate that and understand what you’re saying and ge links you provide I hope people stay away from you.

You know what I just thought of? Call me crazy but I think you may have absent-mindedly stumbled on to something with those words dude. It's almost like they're trying to tell us something in some ancient language. Give me a while and I'll get to the bottom of it.














I keeed I keeeed.

 

[Tinytim]

Visions of women wearing only stained panties and hairy pits chained to tables pressing pills and just scooping caps into a bucket of powder mixed with a paint stirrer.
Definitely some hoo doo voodoo shit

 

[Genotec]

Sterilization refers to any process that eliminates, removes, kills, or deactivates all forms of life and other biological agents (such as fungi, bacteria, viruses, spore forms, prions, unicellular eukaryoticorganisms such as Plasmodium, etc.) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media.[1][2] Sterilization can be achieved through various means, including: heat, chemicals, irradiation, high pressure, and filtration. Sterilization is distinct from disinfection, sanitization, and pasteurization, in that sterilization kills, deactivates, or eliminates all forms of life and other biological agents which are present.

I am sorry English is not my first languange so i cannot always put everything as clearly as i would like to but when it come to sterilisation
there is clear distinction between bacteris and it spors and you are right that sterilisation process can be assest by destroying spores but bacterias not spores die before spore forms are shall i say destroyed.

And while i am at it i can see now who recommeds 180min not 120 :/

 

[mp46]

Sterilization refers to any process that eliminates, removes, kills, or deactivates all forms of life and other biological agents (such as fungi, bacteria, viruses, spore forms, prions, unicellular eukaryoticorganisms such as Plasmodium, etc.) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media.[1][2] Sterilization can be achieved through various means, including: heat, chemicals, irradiation, high pressure, and filtration. Sterilization is distinct from disinfection, sanitization, and pasteurization, in that sterilization kills, deactivates, or eliminates all forms of life and other biological agents which are present.

I am sorry English is not my first languange so i cannot always put everything as clearly as i would like to but when it come to sterilisation
there is clear distinction between bacteris and it spors and you are right that sterilisation process can be assest by destroying spores but bacterias not spores die before spore forms are shall i say destroyed.

And while i am at it i can see now who recommeds 180min not 120 :/

No there is not a clear distinction because the link you just provided groups bacteria and spores into the same category, biological agents. So yet again your link, that you provided says for sterilization purposes bacteria and spores are the same thing.

Look, I don't really care what your processes an procedures are. What I do care about is that you can't seem to keep your shit straight you keep saying one thing and quoting sources that say another thing and then your supplier says something different. Do yourself a favor, just bump that oven up to 200c for 2 hours and be done with it.

 

[HIGHRISK]

Who is dat?

Katie Price

 

[HIGHRISK]

Perfection, im glad Adam donated that rib. Other chicks tits to big, this girl is perfect.
Makes me want to empty my wallet liquidate assets and quit my job, we will travel the world, anything for her. Where do i order?

Remember there’s some guy somewhere tired of her shit! Lol

 

[Genotec]

No there is not a clear distinction because the link you just provided groups bacteria and spores into the same category, biological agents. So yet again your link, that you provided says for sterilization purposes bacteria and spores are the same thing.

Look, I don't really care what your processes an procedures are. What I do care about is that you can't seem to keep your shit straight you keep saying one thing and quoting sources that say another thing and then your supplier says something different. Do yourself a favor, just bump that oven up to 200c for 2 hours and be done with it.

I have done sterilisation/sanitasion course in 2014, 120min reccomended, this fucking link also recommends 120min but as i was reading about this spores i found new who recommendation to keep it for 180min at 160c so i will do as u say 200c for 120min and not worry about it.

 

[mp46]

borosilicate glass is good at 500c. My autoclave says it goes to 180c but I've measured it at 220+ so I just set it on max and let it sit. Let it cool with the door closed so there's no air moving and the glass doesn't cool too fast.

 

[MikeIronTyson]

Remember there’s some guy somewhere tired of her shit! Lol

You are a man of wisdom

 

[mp46]

I will say about the making of the orals; I just realized this morning what a ball mill essentially is. Made correctly, there's really no way to screw it up. As long as he's filtering the oral raws correctly then there's no problem with the raw stage. I won't speak on the mixing of binders and whatever when it comes to making the tabs.

 

[AASraw]

I will say about the making of the orals; I just realized this morning what a ball mill essentially is. Made correctly, there's really no way to screw it up. As long as he's filtering the oral raws correctly then there's no problem with the raw stage. I won't speak on the mixing of binders and whatever when it comes to making the tabs.

May I know whether you have a labs which can be invested? I got money laying died in my account.

 

[Worf]

I don't have the energy for a new source already. It's not even Friday yet. Wtf.

It’s Friday in kasxjacitan

Someone was asking if they were pharma a while back.
The answer is no. Just another UGL from across the ocean.

stay in your own source thread msg labs!

 

[Genotec]

pics

 

[BigBaldBeardGuy]

pics

Oh hey. Welcome back. You think it’s ok to be MIA for 4 days? At the most important time when you’re looking to establish a presence as a “good” source?