Glass filtration kit – how to clean it and keep it sterile?

[Xmen]

Hello! So far, I have used sterile, disposable vacuum filtration kits with a 0.22 micron membrane for filtration. I was wondering whether it would be better to buy a glass filtration set: a bottle or laboratory flask, a clamp, a sintered funnel and sterile membrane filters, closed with a tape. You can sometimes see this in Chinese tutorials. I would use the same pump.

I wonder if any of you use such a set and can advise me how to wash it. Could I use, for example, isopropanol in an ultrasonic cleaner, some cleaning concentrate, roasting at high temperature? Sterilization, of course, involves autoclaving and hot air drying (convection, oven).

Cheers!

 

[helionn]

VF15, Glass Filtration Set Includes 1L funnel, base, clamp, stopper, GL45 adaptor, and 5L bottle

VF15, Glass Filtration Set Includes 1L funnel, base, clamp, stopper, GL45 adaptor, and 5L bottle

www.sterlitech.com

Get this and PVDF filter paper. It will filter things very fast.

Wash with acetone and then wash again with soapy water or alconox if you have it.. Rinse off well with tap water and then rinse that off with distilled water.

 

[shabsofthemoon]

Hello! So far, I have used sterile, disposable vacuum filtration kits with a 0.22 micron membrane for filtration. I was wondering whether it would be better to buy a glass filtration set: a bottle or laboratory flask, a clamp, a sintered funnel and sterile membrane filters, closed with a tape. You can sometimes see this in Chinese tutorials. I would use the same pump.

I wonder if any of you use such a set and can advise me how to wash it. Could I use, for example, isopropanol in an ultrasonic cleaner, some cleaning concentrate, roasting at high temperature? Sterilization, of course, involves autoclaving and hot air drying (convection, oven).

Cheers!

1. Rinse hot water
2. Dishwashing Soap
3. Rinse again + 10% Isopop Alcohol
4. Autoclave
5. Store away

i do steps 3 and 4 before use again