HGH Purity and dimer

[craigdt]

Yeah. Right now I’m on 2iu/day and want to move up to 5 for an upcoming cycle. Looking for cheaper sources but since I’m increasing the dose I don’t want to be adding more crap in with it as well.

Let us know how your higher dose goes.

I tried to work my way up past 2iu and just seemed like my sleep got worse and my hands/feet got swollen.

Had to get one of those rubber wedding rings

 

[NorthMich]

@Ghoul little off topic, but can these filter oils with Test, etc?

 

[Ghoul]

@Ghoul little off topic, but can these filter oils with Test, etc?

.22um PTFE for oils, 25mm, Sterile, and obviously if you want a particle free vial, get the 10ml Ultra Spec.

Syringe Vent Filters Hydrophobic PTFE Autoclavable

Autoclavable hydrophobic PTFE syringe filters. Pore size: 0.2 μm. Diameter of 13mm/25mm/33mm with Luer lock. For venting and air filtration.

shop.cobetter.com

 

[NorthMich]

All ordered up. Super helpful. People like you make this forum an amazing resource

 

[james jwrvey]

So the reason proteins don't have "blind tests" is because of the complexity (and expense) of analyzing a broad spectrum of possibilities. Basically, you have to know what you're looking for, and things like dimer (two hgh proteins attached) and other similar, but not exactly the same, proteins will be detected at the same time.

Pharma has to identify every impurity and ensure they're not something potentially dangerous. That kind of in depth analysis is not practical at a reasonable cost.

So there's 5% of unidentified "stuff".

Statistically, the most likely "impurity" would be aggregates(or aggregate inducing defective/fragmented protein chains, "seeds"). Dimer is a well known type of aggregate, but they can get much larger. Thousands of times larger in some cases.

So if you chose to use this batch, I would use a .22um PES filter, which will eliminate the largest aggregates, since those are the ones most likely to cause issues (an immune response). Do that and it'll probobly be fine.

Sorry to bother you, but say for example someone used stanford as their hgh source, would their hgh be a viable option if you also filter? Also don’t mean to be nosy, but is there any hgh that people or you specifically can actually vouch for? Qsc is gone and i feel like a lost child in a shopping center

 

[rxzed]

Sorry to bother you, but say for example someone used stanford as their hgh source, would their hgh be a viable option if you also filter? Also don’t mean to be nosy, but is there any hgh that people or you specifically can actually vouch for? Qsc is gone and i feel like a lost child in a shopping center

Peptides are largely unaffected. Lobster seems to be the go to for HGH at the moment. It's always a good idea to filter any peptide before using. You're not being nosy, but you should look through threads and use the search bar; you might run across some info that you would otherwise not find.

 

[AlexDavis43]

Peptides are largely unaffected. Lobster seems to be the go to for HGH at the moment. It's always a good idea to filter any peptide before using. You're not being nosy, but you should look through threads and use the search bar; you might run across some info that you would otherwise not find.

It's always a good idea to filter any peptide before using.

Remains to be seen. Before making an overall recommendation, maybe should consider seeing some Jano tests that show extra filtration doesn't cause additional disruptions to the peptides and only removes the aggregates (or does nothing).

 

[rxzed]

Remains to be seen.

I'm just pointing him in the direction of the source that is popular at the moment. Not vouching for or recommending; as always, research should be done before purchasing.

Before making an overall recommendation, maybe should consider seeing some Jano tests that show extra filtration doesn't cause additional disruptions to the peptides and only removes the aggregates (or does nothing).

Not sure I get the second part. I agree that it doesn't cause damage to the peptide. Peptides should always be filtered, as should anything that is purchased from a UGL lab.

 

[AlexDavis43]

Peptides should always be filtered, as should anything that is purchased from a UGL lab.

How long have you felt this way? By putting always in italics, looks like you feel strongly about this. How long have you been advocating UGL peptide filtration

 

[Ateam2023]

Sorry to bother you, but say for example someone used stanford as their hgh source, would their hgh be a viable option if you also filter? Also don’t mean to be nosy, but is there any hgh that people or you specifically can actually vouch for? Qsc is gone and i feel like a lost child in a shopping center

what a nice way to ask to be "spoonfed" a hgh source,, lmao

 

[Scout23358]

Points for being nice though.
Politeness has to count for something!

 

[rxzed]

How long have you felt this way? By putting always in italics, looks like you feel strongly about this. How long have you been advocating UGL peptide filtration

I shouldn't even respond to this, it's so low effort. I do feel strongly about this due to the countless studies on the negative effect of protein aggregation. I feel even more strongly about it due to common sense. You are injecting a substance produced in an unregulated lab that holds no accountability to the sterility and safety of their product.

I'll also address the implication that you won't just outright state. No, this isn't some novel knowledge that I discovered on my own. When someone presents information that you are ignorant to, and has relevance to the safety and efficacy to a practice in which you are doing, you listen and further educate yourself; trust, but verify. This allows you to better form your own practices and recommendations to others. The ad hominem attacks provide nothing of substance.

 

[readalot]

I shouldn't even respond to this, it's so low effort. I do feel strongly about this due to the countless studies on the negative effect of protein aggregation. I feel even more strongly about it due to common sense. You are injecting a substance produced in an unregulated lab that holds no accountability to the sterility and safety of their product.

I'll also address the implication that you won't just outright state. No, this isn't some novel knowledge that I discovered on my own. When someone presents information that you are ignorant to, and has relevance to the safety and efficacy to a practice in which you are doing, you listen and further educate yourself; trust, but verify. This allows you to better form your own practices and recommendations to others. The ad hominem attacks provide nothing of substance.

Appreciate the effort. Save your effort here. Passive aggressive paradise for those that like that sorta thing.

 

[readalot]

Would be interesting to do before and after 0.2 um filtration for higher MW oliogomers...

Analysis of Somatropin According to EP Method (LW-803) - Shodex

Somatropin is a genetical recombinant natural type human growth hormone preparation, a peptide consisting of 191 amino acids (molecular weight about 22,000).

shodexhplc.com

Caught some tetramer here.

 

[brutalbuilt]

Sorry to bother you, but say for example someone used stanford as their hgh source, would their hgh be a viable option if you also filter? Also don’t mean to be nosy, but is there any hgh that people or you specifically can actually vouch for? Qsc is gone and i feel like a lost child in a shopping center

lots of other good sources for hgh out there... i had the most water retention with qsc compared to any other source ive used before i didnt like it

 

[readalot]

https://www.sciencedirect.com/science/article/pii/S0378517323002491

Probably going to have to look pretty hard.

 

[clearheaded]

how do we know not smashing peptides under pressure through tiny holes isn't causing destruction and more aggregates??? which is pretty likely depending on peptide. ie ur filtering could make things worse. jaaannoooo

 

[songsofpyramids]

how do we know not smashing peptides under pressure through tiny holes isn't causing destruction and more aggregates??? which is pretty likely depending on peptide. ie ur filtering could make things worse. jaaannoooo

There’s studies showing no issues filtering hgh at .2um. Filtering is also standard practice at compounding facilities for glp1. There actually is quite a lot of hard science suggesting filtering peptides is the way to go. I’m too lazy to dig them up but they’re on here if you search for it.

 

[Ghoul]

how do we know not smashing peptides under pressure through tiny holes isn't causing destruction and more aggregates??? which is pretty likely depending on peptide. ie ur filtering could make things worse. jaaannoooo

Because:

1: Peptides are far smaller than .2um

2: .2um filtration is a standard manufacturing step in pharmaceutical peptide production

3. Every commercial producer of peptides advises .2um filtration where sterility is desired.

 

[Ghoul]

Any ONE of these factors is sufficient reason to filter as a means of harm reduction. The onus is on those who insist filtering is useless or increases harm to make their case.

1. UGL does nothing to ensure they supply particulate free compounds or vials. Injected glass, plastic, and other sub-visible particulates can migrate to organs, accumulate, remain permanently, cause micro emboli, inflammation, and other unknown harms.

2. UGL does nothing to control aggregation or measure the effect of the resulting immunogenic response. Filtering eliminates aggregates above .2um in size. The larger the aggregate, the greater the risk of negative consequences. 10um and larger aggregates occlude blood flow. Visible aggregates(and other particulates), the type seen in cloudy reconstituted solutions, are over 100um.

3. A significant proportion of peptides have failed sterility tests. .2um filtration ensures sterility. This is also standard laboratory practice for any peptides intended for use in human or animal subjects. Are you not worth the same measures used to protect the health of a lab rat?