HGH Purity and dimer

[clearheaded]

I feel like adding the reality that filtering ur likely adding MORE aggregates pretty important if the goal is less aggregates... er no? lol

I don't need to name call..... lol

anyway, obv ur just a trolling...so silly for a grown man

 

[Bobertson]

Any thoughts on type of filter? I have some PES .22 filters I picked up from a med supply sale. Or should you use ptfe only?

 

[Bobertson]

Any thoughts on type of filter? I have some PES .22 filters I picked up from a med supply sale. Or should you use ptfe only?

0.2 Micron Filter, sterile, J1081

Product DescriptionFilter is used when transferring serum to 6mL syringes. Procedures will require multi filters. Syringe not included.This filter has a female luer lock and male luer lock fitting. Green. 22mm. Sterile. Packaged singles.

www.shopmedvet.com

These ones specifically

 

[songsofpyramids]

@clearheaded

I can’t help you because apparently the search function is over your head, and I won’t help you because you’re a childish asshole who literally jumped into the conversation with insults.

If you want people to help you find information it would probably be a better call to not act like a petulant know it all asshole from jump while citing laughably sophomoric and incoherent studies to the topic at hand. Being friendly will get you a lot further in life. A lesson your parents failed to teach you apparently.

 

[songsofpyramids]

Any thoughts on type of filter? I have some PES .22 filters I picked up from a med supply sale. Or should you use ptfe only?

I can’t speak to the brand you’re using but .22um PES filters are indeed what you’re looking for because the least amount of protein binds to them.

 

[Ghoul]

0.2 Micron Filter, sterile, J1081

Product DescriptionFilter is used when transferring serum to 6mL syringes. Procedures will require multi filters. Syringe not included.This filter has a female luer lock and male luer lock fitting. Green. 22mm. Sterile. Packaged singles.

www.shopmedvet.com

These ones specifically

I recognize this, it's a B Braun filter, the proper filter media for low binding of protein, PES. In fact, it's "Supor", a premium type of PES favored in the pharma industry,

The only downside is the relatively high .3ml of fluid retention. This makes an "air purge" to get most of that out to minimize loss essential, So when done pushing the liquid through, remove the filter, pull at least 1 ml air into the syringe, reattach filter, and push through to recover the trapped liquid. In addition, the greater the dilution the less this retention matters, so unless it would make the injection volume too large for your dose, it would be best to reconstitute with 3ml.

0.2 Micron SUPOR^® Aspiration / Injection Disc Filter

I'll be posting some data soon, and if I recall, this filter was tested, and performed well for removing aggregates from peptides,

 

[clearheaded]

@clearheaded

I can’t help you because apparently the search function is over your head, and I won’t help you because you’re a childish asshole who literally jumped into the conversation with insults.

If you want people to help you find information it would probably be a better call to not act like a petulant know it all asshole from jump while citing laughably sophomoric and incoherent studies to the topic at hand. Being friendly will get you a lot further in life. A lesson your parents failed to teach you apparently.

again, I searched, all came up was ghoul.. this is a VERY feeble attempt my guy(go prove urself something)... ALSO, you are just ignoring study that says it CREATE aggregates? that doesn't count? lol come on

YOU CAME AT ME lol so again clearly just a troll...

 

[clearheaded]

Investigation on fouling mechanisms for recombinant human growth hormone sterile filtration - PubMed

During sterile filtration of recombinant human growth hormone solutions, severe membrane fouling was experienced compared to other protein preparations of significantly higher molecular weights and concentrations. This phenomenon was attributed to rhGH aggregation/adsorption occurring in the...

pubmed.ncbi.nlm.nih.gov

"This phenomenon was attributed to rhGH aggregation/adsorption occurring in the filter pore. .... The protein solutions were sterily filtered using 0.22 mm filters,"

 

[Bobertson]

I recognize this, it's a B Braun filter, the proper filter media for low binding of protein, PES. In fact, it's "Supor", a premium type of PES favored in the pharma industry,

The only downside is the relatively high .3ml of fluid retention. This makes an "air purge" to get most of that out to minimize loss essential, So when done pushing the liquid through, remove the filter, pull at least 1 ml air into the syringe, reattach filter, and push through to recover the trapped liquid. In addition, the greater the dilution the less this retention matters, so unless it would make the injection volume too large for your dose, it would be best to reconstitute with 3ml.

0.2 Micron SUPOR^® Aspiration / Injection Disc Filter

I'll be posting some data soon, and if I recall, this filter was tested, and performed well for removing aggregates from peptides,

Nice. Thanks. I lucked out and got these for $2 a pop.

I had planned on just running bac water through at the end to flush it. E.g mix 2.25ml and filter that into the target receptacle. And then just flush the filter with plain bac water to get to 3ml in the target receptacle. .

Is the air purge better than that?

 

[Sampei]

I recognize this, it's a B Braun filter, the proper filter media for low binding of protein, PES. In fact, it's "Supor", a premium type of PES favored in the pharma industry,

The only downside is the relatively high .3ml of fluid retention. This makes an "air purge" to get most of that out to minimize loss essential, So when done pushing the liquid through, remove the filter, pull at least 1 ml air into the syringe, reattach filter, and push through to recover the trapped liquid. In addition, the greater the dilution the less this retention matters, so unless it would make the injection volume too large for your dose, it would be best to reconstitute with 3ml.

0.2 Micron SUPOR^® Aspiration / Injection Disc Filter

I'll be posting some data soon, and if I recall, this filter was tested, and performed well for removing aggregates from peptides,

What I found with the airpurge is that when it's purging at the end it creates a white foam due to the pressure and the air getting push through the filter. Isn't that a massive bunch of aggregations?

 

[Ghoul]

Nice. Thanks. I lucked out and got these for $2 a pop.

I had planned on just running bac water through at the end to flush it. E.g mix 2.25ml and filter that into the target receptacle. And then just flush the filter with plain bac water to get to 3ml in the target receptacle. .

Is the air purge better than that?

No, your idea is better. The air purge thing is a common lab technique, but given the fact "air/liquid interface" is one of that many factors contributing to aggregation, avoiding it with a BAC flush that ends in the desired reconstitution volume is a smarter way to go imo.

We have some way to go before establishing a standard "best practice" method for those of us doing this on the UGL scene. I expect it'll continue being tweaked and improved for quite some time.

It's still a developing science even for biotech professionals and the FDA.

 

[clearheaded]

There’s studies showing no issues filtering hgh at .2um. Filtering is also standard practice at compounding facilities for glp1. There actually is quite a lot of hard science suggesting filtering peptides is the way to go. I’m too lazy to dig them up but they’re on here if you search for it.

BAHAHAH u think compounding pharmacies filter glp1 lyophilized peptides? they would filter if give u liquid JUST for sterility.. sterility is different than aggregates. Just FYI

 

[clearheaded]

No, your idea is better. The air purge thing is a common lab technique, but given the fact "air/liquid interface" is one of that many factors contributing to aggregation, avoiding it with a BAC flush that ends in the desired reconstitution volume is a smarter way to go imo.

still await ur study...... again, very much possible creating MORE aggregates and loosing alot of HGH...

 

[songsofpyramids]

@clearheaded - I’m sorry you’re so fucking dumb and childish kid. Nothing any of us can do to help with that.

This behavior is exactly why I didn’t think you were mature enough to provide anything of meaning or substance to the conversation btw. Thank you for validating your own uselessness.

 

[clearheaded]

@clearheaded - I’m sorry you’re so fucking dumb and childish kid. Nothing any of us can do to help with that.

This behavior is exactly why I didn’t think you were mature enough to provide anything of meaning or substance to the conversation btw. Thank you for validating your own uselessness.

lol so u really thought compounding pharm filtered lyophilized peptides they sell... just curious.

 

[songsofpyramids]

Go cry somewhere else kid

 

[clearheaded]

only tantrum thrower is you my friend good luck!

 

[songsofpyramids]

Your parents obviously failed at the most basic steps of making a man. It’s no wonder you’re always such a whiny asshole to everyone.

 

[Bobertson]

No, your idea is better. The air purge thing is a common lab technique, but given the fact "air/liquid interface" is one of that many factors contributing to aggregation, avoiding it with a BAC flush that ends in the desired reconstitution volume is a smarter way to go imo.

We have some way to go before establishing a standard "best practice" method for those of us doing this on the UGL scene. I expect it'll continue being tweaked and improved for quite some time.

It's still a developing science even for biotech professionals and the FDA.

Thanks for your help man. Glad to have some knowledgeable people here.

I’ll put it to use on the 10x Lobster China kit I just ordered. I don’t trust it anywhere near as much as my regular source. Prices too good to be true out of nowhere, a bit too much of a flash in the pan. If this sticks around, maybe it will lead to better prices elsewhere.

 

[clearheaded]

Your parents obviously failed at the most basic steps of making a man. It’s no wonder you’re always such a whiny asshole to everyone.

please stop trolling like a 15 yo. you discredit urself.

the silence of @Ghoul is palpable, lol....

at any rate. proceed with knowledge ur syringe filters not only possibly but likely create more aggregates, but lower level of active compounds. the question is just how much, and is it worth it for sterility? or is it worth it for chance there is GIANT bioactive aggregates that the 0.2 can catch and pushing more peptide over large aggregates creates MORE aggregation, not less. logic.

id suggest getting large vials if want to filter you HGH so you loose less AND less likely to create more aggregates. since we are just making things up, that would make the most sense. adding surfactant(non ionic) would help like polysorbate 20 (possible china already adds it I have no idea). check ph so optimal dissolution and prevention of aggregates.