MESO-Rx
Anabolic Steroids
MALDI-TOF-MS/HPLC-UV-VIS rHGH results
- Thread starter mands
- Start date
Jim, this is the task the Lab was given for the GodTropin/AngTropin samples:
PROTEIN CHARACTERIZATION:
Up to 50,000 sequencing events. Includes UPLC-MS/MS on Q-Exactive Orbitrap via unbiased, data-dependent acquisition, inclusive of sample preparation and parallel multi-enzyme digestions using six enzymes, sequence library searching, relative quantitation and reporting via Proteome Cluster
Intact protein mass measurement, fragmentation and sequencing by UPLC-MS/MS on Q-Exactive Orbitrap, 90 m, deconvolution of up to 20 mass envelopes
Each sample came with a full 10 page report
I'd like your opinion on the results posted. I'd hate to think we were getting duped by the lab. The lab is an accredited lab.
The first question one should always have is the LAB itself and the second is the veracity of the GH supplier.
In the latter instance how do you KNOW what was tested was indeed the "generic" GH it was claimed to be.
With regard to the lab I'll tell you the variance in GH levels using the same dose of GH is highly suspect in and of itself. (Also bc IGF levels require a 4-6 week stabilization interval, obtaining such data at ten days is clearly a deviation from the accepted norm)
Moreover, at a glance for starters, there is a shit load of procedural info that is notably missing such as the, solvents used, and more importantly the sensitivity of the MS and HPLC equipment itself.
And let's see here has everyone overlooked the fact they didn't post THEIR STANDARD?
If it's good for the goose it's good for the gander, but it's all good bc it's Eroids, a SOURCE BOARD, LMAO!
Buyer beware IMO!
In the latter instance how do you KNOW what was tested was indeed the "generic" GH it was claimed to be.
With regard to the lab I'll tell you the variance in GH levels using the same dose of GH is highly suspect in and of itself. (Also bc IGF levels require a 4-6 week stabilization interval, obtaining such data at ten days is clearly a deviation from the accepted norm)
Moreover, at a glance for starters, there is a shit load of procedural info that is notably missing such as the, solvents used, and more importantly the sensitivity of the MS and HPLC equipment itself.
And let's see here has everyone overlooked the fact they didn't post THEIR STANDARD?
If it's good for the goose it's good for the gander, but it's all good bc it's Eroids, a SOURCE BOARD, LMAO!
Buyer beware IMO!
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THE LAB:
Provider of contract research, specializing in biological mass spectrometry and informatics.
They provide research for over 400 Institutions (Baylor College of Medicine, Harvard Medical School, Johns Hopkins, etc)
http://planetorbitrap.com/q-exactive#.Vfxp5nD3aK0
THE SAMPLES:
The samples sent in for analysis were from the same kit(s) used for the IGF1 Serum testing.
BLOOD WORK:
The variance in IGF1 Serums seem to indicate that SIMEC.ch is correct with their finding (AngTropin - 17iu vial)
This is just my opinion.
The "accepted norm" protocol was not used of course. After reading actual clinical studies (IGF1 Serums, Bioequivalence), we know now that IGF1 can be tested like the infamous GH Serum Protocol. 4-6 weeks isn't needed for elevation and IGF1 Serum can be doubled from only one SubQ inject as long as a "baseline" is established for comparison.
This information is very old:
http://www.somatropin.cn/hghtest.html
GH SUPPLIERS:
Yes, the product can vary from batch to batch as there is no "quality control" for black market GH. For this reason, I hope you find a way to continue testing. It really is appreciated and beneficial to this community.
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Again I just gendered at the assays but will give them a closer look if you like.
Most lab errors occur bc the folk performing the assays are either not qualified, or not trained accordingly.
Most lab errors occur bc the folk performing the assays are either not qualified, or not trained accordingly.
xmerola85
New Member
are plat directs greytop the same as the hk ? are hks better ? i am trying to read through all the pages just found this
They are indeed from the same manufacturer in China.
Thanks for all those who were patient, but bc of certain logistical issues the standards were no longer available to use for comparison.
So what to do? Post the MOST ACCURATE means of assaying the concentration of those protein samples in question.
Oh they were never doubted by yours truly, yet there are those whom have a vested interest in GH sales on Professional Muscle and would like nothing more than to post their uneducated "objections" to each and every GH test I've conducted.
Briefly using Avagadro's Number Amino Acid Analyses determine the number of moles contained within a sample and that quantity is then converted into milligrams and from there milligrams IN EACH SAMPLE VIAL!
There is NO TEST MORE ACCURATE, and this data CONFIRMS your selling under dosed GH on PM MEATHEAD!
So what to do? Post the MOST ACCURATE means of assaying the concentration of those protein samples in question.
Oh they were never doubted by yours truly, yet there are those whom have a vested interest in GH sales on Professional Muscle and would like nothing more than to post their uneducated "objections" to each and every GH test I've conducted.
Briefly using Avagadro's Number Amino Acid Analyses determine the number of moles contained within a sample and that quantity is then converted into milligrams and from there milligrams IN EACH SAMPLE VIAL!
There is NO TEST MORE ACCURATE, and this data CONFIRMS your selling under dosed GH on PM MEATHEAD!
muscle96ss
Subscriber
For christs sake Jim, no more excuses and bullshit, just let it be!!!
Damn so 2 samples testing close to ~19mgs/ml. One at less than 70% of that, and a 4th sample that was basically funny smelling water?
What would a good score per ml be? I don't know much about those test or much about that many at all. Thanks grey
Honestly not sure... @mands and co here would know better than I. The inconsistency certainly isn't great though.
A "good score" would be for these GH vials to contain what's on the label...... TEN IU
Here is the amount of GH contained, in mg, of GH in EACH VIAL!
1) 2.77
2) 2.0
3) 2.2
4) 0.9
Now multiply times THREE to obtain IUs per vial.
ALL ARE UNDER DOSED!
Here is the amount of GH contained, in mg, of GH in EACH VIAL!
1) 2.77
2) 2.0
3) 2.2
4) 0.9
Now multiply times THREE to obtain IUs per vial.
ALL ARE UNDER DOSED!
Thanks Jim, I am very Grateful for these test and all your time Doc....
Yes great infos but which brands are they ?
Where the fuck have you been? Go sit in the corner, You are in time-out.. .
Try reading the THREAD would ya!
Colt44
Well-known Member
What's the score of the sample I sent in?
I did not run an AA analysis on it. I've spent enough money on this project as is.
Hey I've an idea have Meathead pay for it, LOL!
I do wonder however if such high LOADS of glycine are responsible for some of the symptoms so common to generic GH, ESPECIALLY when one considers the fact NO PHARM GRADE GH has more than a few mg!
Hey I've an idea have Meathead pay for it, LOL!
I do wonder however if such high LOADS of glycine are responsible for some of the symptoms so common to generic GH, ESPECIALLY when one considers the fact NO PHARM GRADE GH has more than a few mg!
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One could assume underdosed GH with overdosed glycine could still give great sleep.. ..
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