MALDI-TOF-MS/HPLC-UV-VIS rHGH results

isn't TP cheaper? I tried to use PD but it's so retarded, He ship from europe but don't ship from europe to europe.... lol
 
isn't TP cheaper? I tried to use PD but it's so retarded, He ship from europe but don't ship from europe to europe.... lol
Maybe cheaper by a few dollars. Inside the states nobody is faster. Dealing with pd is easy and his t/a is on point.
 
@mands What is the protocol you want for the IGF-1 testing? It would probably be more then a sample for IGF-1 now that I think about it. You could get away with a sample for serum. Anyways I am assuming most of you didn't get a baseline and have all jumped on already. Not being critical I have done the same thing but just trying to figure out how to keep this thing going. I am a fan of this if you guys find that needle in the haystack I want to save money too. If you were one who sent a sample in PM me I have an idea or two.
 
@mands What is the protocol you want for the IGF-1 testing? It would probably be more then a sample for IGF-1 now that I think about it. You could get away with a sample for serum. Anyways I am assuming most of you didn't get a baseline and have all jumped on already. Not being critical I have done the same thing but just trying to figure out how to keep this thing going. I am a fan of this if you guys find that needle in the haystack I want to save money too. If you were one who sent a sample in PM me I have an idea or two.
Obviously base lines are a must in my opinion. Some will suggest that it's not necessary.

After base lines are taken then bloods need to be taken around the 3-5 weeks mark of steady GH use. I personally think a consistent 3 week run is ample time but some like to see 5 weeks.

I believe PrivateMD labs have the IGF-1 testing available for around $75.00.

Hope this helps.

Oh by the way. I had a little bit of a surprise in my email this weekend. The lab pushed through all 5 samples I sent.

1. Anhul Anke Biotechnology - Grey Top(Flip off)
2. Gen Sci Jintropin - Green Top
3. TP - The Grey Tops
4. Mean Green - Green Tops
5. Genotropin - Pfizer and used as new standard.

I will post the MS a little later when I can get back on. Have meetings today.

mands
 
I have again a question, can we understand how many mg there is of HGH in a vial. I was looking at somastim from sciroxx.

The purity is 99% lol but what about the quantity in each vial?
 
Since nobody commented on the thread, can we get some feedback on this? Thoughts? The guy that put this togethere is someone I highly respect and has a wealth of knowledge.



https://www.eroids.com/pics/igf1-vs-gh-serum @Dr JIM

I'll bite. I think its obvious that the best tests in order of importance are 1.) actual lab tests, 2.) IGF-1, and 3.) serum GH. However, in terms of price and ease the serum GH wins hands down. I mean who wants to waste time running a bunch of GH for a couple weeks before doing an IGF-1 when you can simply do a serum test beforehand and get a good idea of whether or not what you have is real or not.

I also have a point of contention with the article. They state that serum GH numbers from different vials within the same kit were 7.2, 8.0, 30.5, and 13.3 and therefore serum numbers have much variability. I would put a huge wager that the reason the numbers were so variable was because of krapp GH and not because of user variability(will go into this further if anyone wants me to). If you follow Racepick's thread on PM(I know, everybody here hates PM); you will see that there is variability between users but consistency among the same user. In fact RP has done a shitload of serums on the greys and they have all been within a couple points of each other. I have done the same as well personally with a couple different GH's. In fact of all the serums I have done where I have done a follow-up with an IGF-1 as well; there has been a definite correlation where the lower serums have lower IGF-1's and the higher serums have higher IGF-1's. So I think serums have a huge value in initially telling the user whether it is worthwhile to use the GH. However, you must have establish an individual history for the numbers to really have meaning.
 
I have again a question, can we understand how many mg there is of HGH in a vial. I was looking at somastim from sciroxx.

The purity is 99% lol but what about the quantity in each vial?

NOT wo conducting an HPLC! And you bet that's one way UGL cheat on their generic GH products. List the "kit" as containing TEN vials with TEN IU in each one but only put 3-4 IU in each vial, the remainder being "filler" agents.

This is usually why some folks are told by generic manufacturers to use TEN IU from the outset, bc while the product may be of high purity it's CUT down 60-70%, so in effect only THREE IU is being used.

Don't believe me try TEN IU OF HUMATROPE LMAO, Crap I couldn't get past ONE IU before CTS became debilitating.

Of course I'm not suggesting my HT experience is the norm for younger folk bc I know it's not but adverse effects are greatly diminished when the dose gradually increased. That's why you will find the above as a dosing guideline by pretty much every PH grade GH producer.
 
Since nobody commented on the thread, can we get some feedback on this? Thoughts? The guy that put this togethere is someone I highly respect and has a wealth of knowledge.



https://www.eroids.com/pics/igf1-vs-gh-serum @Dr JIM

Why of course that's true. The tests are complimentary BUT a rough estimate of the amount of GH used CAN be ascertained using Ph grade data as the standard.

However IGF testing can accomplish what no other assay can,, PROVE THE GH YOUR USING IS BIOLOGICALLY ACTIVE!
 
NOT wo conducting an HPLC! And you bet that's one way UGL cheat on their generic GH products. List the "kit" as containing TEN vials with TEN IU in each one but only put 3-4 IU in each vial, the remainder being "filler" agents.

This is usually why some folks are told by generic manufacturers to use TEN IU from the outset, bc while the product may be of high purity it's CUT down 60-70%, so in effect only THREE IU is being used.

Don't believe me try TEN IU OF HUMATROPE LMAO, Crap I couldn't get past ONE IU before CTS became debilitating.

Of course I'm not suggesting my HT experience is the norm for younger folk bc I know it's not but adverse effects are greatly diminished when the dose gradually increased. That's why you will find the above as a dosing guideline by pretty much every PH grade GH producer.


But it was done an hplc.

By mands and you: results
Anyone can explain what's the concentration in that vial?
 
While Ks GH had a MW close to Ph grade at 22113 D vs 22131 there is only 1.36 mg of his protein in each vial.

So that means although folk may believe they are pinning TEN IU, if the entire vial is used, they are actually injecting roughly FOUR IU.

Moreover bc this product is NOT entirely consistent with rHGH it's Biologic effects ARE UNKNOWN.

He already explained here.^^^

But it was done an hplc.

By mands and you: results
Anyone can explain what's the concentration in that vial?

Here are your key words that are listed on the HPLC results. (Second file for each sample)

The observed concentration of the sample solution = 1.36 mg/ml ± 0.05 mg/ml.

It's not listed in iu but in mg.

Approximately .33mg = 1iu or 1mg= 3iu. The sample contains 1.36/.33, which is equal to 4.1212. So 4iu @ a purity of 99%. The sample contained less than half of the listed concentration, if they were claiming 10iu but if i'm not mistaken those were listed as 20iu? Have to ask Mands.

So more than likely the "manufacturer" cut Ph grade GH in half, threw in some filler, and rebottled it to allow for a larger profit margin. Who is really going to know without running these tests?
 
He already explained here.^^^



Here are your key words that are listed on the HPLC results. (Second file for each sample)

The observed concentration of the sample solution = 1.36 mg/ml ± 0.05 mg/ml.

It's not listed in iu but in mg.

Approximately .33mg = 1iu or 1mg= 3iu. The sample contains 1.36/.33, which is equal to 4.1212. So 4iu @ a purity of 99%. The sample contained less than half of the listed concentration, if they were claiming 10iu but if i'm not mistaken those were listed as 20iu? Have to ask Mands.

So more than likely the "manufacturer" cut Ph grade GH in half, threw in some filler, and rebottled it to allow for a larger profit margin. Who is really going to know without running these tests?
Those were 10 iu's Colt.

I need to talk to Jim because I have received questions on the concentration .. They question the amount... Below states that a portion of a larger amount is added to a solution and then a portion was injected for testing. So the question is if the whole portion was used would it show a higher concentration.

A rHGH standard with a known amount of protein was used for the calculation. In both cases, AS1743-1 and the protein standard solution, aliquots of 10 µl were added to 40 µl ultrapure water. An aliquot of 25 µl was injected.

3.72 mg/ml (GH standard) / 52,121,286 (peak area)} x 19,022,564 = 1.36 mg/ml.

DrJIM will explain better.

mands
 
Those were 10 iu's Colt.

I need to talk to Jim because I have received questions on the concentration .. They question the amount... Below states that a portion of a larger amount is added to a solution and then a portion was injected for testing. So the question is if the whole portion was used would it show a higher concentration.

A rHGH standard with a known amount of protein was used for the calculation. In both cases, AS1743-1 and the protein standard solution, aliquots of 10 µl were added to 40 µl ultrapure water. An aliquot of 25 µl was injected.

3.72 mg/ml (GH standard) / 52,121,286 (peak area)} x 19,022,564 = 1.36 mg/ml.

DrJIM will explain better.

mands

they should inject the WHOLE aliquot so we know the concentration/quantity.
or we should calculate it.

Am I wrong? I don't really understand it :(
 
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