MESO-Rx

Anabolic Steroids

MALDI-TOF-MS/HPLC-UV-VIS rHGH results

Sampei said:
they should inject the WHOLE aliquot so we know the concentration/quantity.
or we should calculate it.

Am I wrong? I don't really understand it :(
Click to expand...

Yes YOU ARE WRONG!

Some of you fellas seem to be over thinking what IS the lab STANDARD FOR ANALYTICAL TESTING. Colts earlier post is EXACTLY CORRECT.

Oh and I suspect some (UGL I assume) question the concentrations being listed in this thread, but they are accurate and I have no doubt about it. If the involved labs don't like it, then they are entitled to post their own studies to refute this data. Don't hold your breath, lol!
 
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Sampei said:
Sampei said:
I have again a question, can we understand how many mg there is of HGH in a vial. I was looking at somastim from sciroxx.

The purity is 99% lol but what about the quantity in each vial?
Click to expand...

But it was done an hplc.

By mands and you: results
Anyone can explain what's the concentration in that vial?
Click to expand...

Your not making any sense bc my response was in reference to a Sciroxx GH product and I've NOT assayed any which means an HPLC would not have been performed.

So what the heck are you referring to an HPLC Sciroxx posted? Good luck with that one if such is the case.
 
Dr JIM said:
Your not making any sense bc my response was in reference to a Sciroxx GH product and I've NOT assayed any which means an HPLC would not have been performed.

So what the heck are you referring to an HPLC Sciroxx posted? Good luck with that one if such is the case.
Click to expand...

He's referring the western bio sample that is from Karl (Sciroxx owner), indirectly. I believe you 2 have had words? Lol
 
jim can you stop being so fucking aggressive? we are on the same team here for fuck sake! But I don't do HPLC at work, I don't know shit about how to read these test.

So stop being a prick, and try to answer question from us poor retard in a way that maybe we understand it.
thank you
 
Well yea even though my stereoscopic spectrographic X-ray vision is probably more than adequate, is that better.

FUCK OFF and read some instead of needing to be spoon fed every damn time you post a "question".
 
^^^ You'd get tired of explaining the same shit over and over also. The information is all there.

Try searching for assays of proteins using a reference standard is where I would start. Maybe dilution factors also. That will keep you busy for a long while, trust me. Just a thought.

If you did this and posted some sort of informed response or question, I'm sure Jim or anyone with knowledge of these assays wouldn't mind pointing you in the right direction.

Sampei said:
jim can you stop being so fucking aggressive? we are on the same team here for fuck sake! But I don't do HPLC at work, I don't know shit about how to read these test.

So stop being a prick, and try to answer question from us poor retard in a way that maybe we understand it.
thank you
Click to expand...
 
Man I tried and I got like 100 pages to read of stuff that I'm not even educated of.

I didn't want a scientific explanation just to answer what mands said as well.

If they use only a part of the solution would the quantity concentration change if they were going to use all of it?

And it's not about being tired to answer, half of Jim post towards me was almost like I was trying to discredit the test or whatever. I said it plenty of time: I'm definitely wrong, just don't know where.

Anyway let's forget about it, and back on topic :)

Mands tell us something, is grey top good? Or not? Come on man
:( give us something ahah
 
Sampei said:
Man I tried and I got like 100 pages to read of stuff that I'm not even educated of.

1) I didn't want a scientific explanation just to answer what mands said as well.

2) If they use only a part of the solution would the quantity concentration change if they were going to use all of it?

And it's not about being tired to answer, half of Jim post towards me was almost like I was trying to discredit the test or whatever. I said it plenty of time: I'm definitely wrong, just don't know where.

Anyway let's forget about it, and back on topic :)

Mands tell us something, is grey top good? Or not? Come on man
:( give us something ahah
Click to expand...

I will try one more time but ...........

1) Mands question was about how UGLs were "doubting" the concentration results. Well now why do YOU think they would do such a thing, bc the data I've posted clearly shows many are selling an UNDERDOSED PRODUCT. That's to say, although their label states there is 10 IU in each vial they usually contain considerably less. The actual amount varies from 2-6 IU in each vial for most generic GH products I've tested

2) Im really having difficulty grasping what this is related to but depending upon the substance being analyzed different solvents are used to FULLY DISSOLVE the sample under question. That's bc using a supersaturated sample (and in a few instances an under-saturated sample) can cause equilibrium shifts which will effect the concentrations reliability.

3) The following are reasons an entire 1 ml vial CAN NOT or NEED NOT be tested
-All HPL analyses required multiple runs to improve accuracy
- Large volume injections can exceed the HPLCs pressure or volume tolerances
- Either can cause a grid blow out, a situation in which the pressure alone FORCES the sample thru the grid and down the DRAIN
- Micro liter sampling has become the norm since the adaptation of High Pressure (Low Volume) Liquid Chromatography systems. The primary reason HPLCs are used is the minute sample requirements.
- Finally providing the sample constituents are in equilibrium with the solvent being utilized the results, which are based on WELL ESTABLISHED DILUTION FACTORS, will be VERY ACCURATE with a margin of error approximating 0.05 mg/ml, as was listed in this instance!
 
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Dr JIM said:
I will try one more time but ...........

2) Im really having difficulty grasping what this is related to but depending upon the substance being analyzed different solvents are used to FULLY DISSOLVE the sample under question. That's bc using a supersaturated sample (and in a few instances an under-saturated sample) can cause equilibrium shifts which will effect the concentrations reliability.

3) The following are reasons an entire 1 ml vial CAN NOT or NEED NOT be tested
-All HPL analyses required multiple runs to improve accuracy
- Large volume injections can exceed the HPLCs pressure or volume tolerances
- Either can cause a grid blow out, a situation in which the pressure alone FORCES the sample thru the grid and down the DRAIN
- Micro liter sampling has become the norm since the adaptation of High Pressure (Low Volume) Liquid Chromatography systems. The primary reason HPLCs are used is the minute sample requirements.
- Finally providing the sample constituents are in equilibrium with the solvent being utilized the results, which are based on WELL ESTABLISHED DILUTION FACTORS, will be VERY ACCURATE with a margin of error approximating 0.05 mg/ml, as was listed in this instance!
Click to expand...

You know, I actually found ALL of that when researching. :) I'm being somewhat of a smartass but I actually did.
 
Holy Shit!! Are the Grey Tops: a)total shit....b)pretty good shit. C)get some ASAP, Fire Shit!!! Thanks for all the help y'all!!! TREN4President!!!"
 
Dr JIM said:
I will try one more time but ...........

1) Mands question was about how UGLs were "doubting" the concentration results. Well now why do YOU think they would do such a thing, bc the data I've posted clearly shows many are selling an UNDERDOSED PRODUCT. That's to say, although their label states there is 10 IU in each vial they usually contain considerably less. The actual amount varies from 2-6 IU in each vial for most generic GH products I've tested

2) Im really having difficulty grasping what this is related to but depending upon the substance being analyzed different solvents are used to FULLY DISSOLVE the sample under question. That's bc using a supersaturated sample (and in a few instances an under-saturated sample) can cause equilibrium shifts which will effect the concentrations reliability.

3) The following are reasons an entire 1 ml vial CAN NOT or NEED NOT be tested
-All HPL analyses required multiple runs to improve accuracy
- Large volume injections can exceed the HPLCs pressure or volume tolerances
- Either can cause a grid blow out, a situation in which the pressure alone FORCES the sample thru the grid and down the DRAIN
- Micro liter sampling has become the norm since the adaptation of High Pressure (Low Volume) Liquid Chromatography systems. The primary reason HPLCs are used is the minute sample requirements.
- Finally providing the sample constituents are in equilibrium with the solvent being utilized the results, which are based on WELL ESTABLISHED DILUTION FACTORS, will be VERY ACCURATE with a margin of error approximating 0.05 mg/ml, as was listed in this instance!
Click to expand...

Now i got it :)
SO the smart ass on eroids that produce somastim is trying to make the test looks like it's 99% purity with even OVERDOSED quantity of 11IU, let me get there and blast him :)
thank you,
 
GodTropin (yes...that is correct, GodTropin :))

I'd like to have that tested (relabeled BlueTop)

GodTropin.net
GodTropin.com
 
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