One Pharma: Bulk Cyp

[Dr JIM]

I deleted the post for personal reasons BUT Ive already voiced my disappointment about posting purity data using MS ALONE.

Primarily bc there is no basis in the literature for such an analysis, absent a HPLC/LC OR a total ion MS scan with a reference standard, which the posted MS is NOT!

 

[MassTurk]

@MythotiK dr Jim seems to believe there is missing data in this report to make the purity claim. Any thought ??

 

[rutman]

I deleted the post for personal reasons BUT Ive already voiced my disappointment about posting purity data using MS ALONE.

Primarily bc there is no basis in the literature for such an analysis, absent a HPLC/LC OR a total ion MS scan with a reference standard, which the posted MS is NOT!

Many thanks Dr Jim for bringing this point back up. I felt like you made a valid point about what's not being shown in the test. Didn't mean to step on your toes...just thought we needed further elaborations and answers on this from the experts.

 

[MythotiK]

He's an idiot!!!! Plain and simple, I won't even indulge him in discussing it either!!!!!

And the fact that I have lots of reports, he has none!!! Well he has a few but they are bullshit reports that have compounds you would NEVER see in AAS!!!! Want to waste your money, please send all you compounds to Jim for testing....

-Myth-

 

[Dr JIM]

@MythotiK dr Jim seems to believe there is missing data in this report to make the purity claim. Any thought ??

Well in fairness I believe Myth or whomever performed this test should be given an opportunity to respond. It's just that I always prefer giving established Meso members the benefit of the doubt, especially a mate like Myth!

Regs
JIM

 

[CensoredBoardsSuck]

Looks like nobody cares what the 28% inside is, since the purity was 72%.

I hope the Chinese did not piss on it.

That's a important point so I'm not sure why you're catching flack for pointing it out.

Since the raws came from the Chinese, the unknown contents could be anything from flour to melamine. I suspect most of them cut their raws with whatever they have laying around that's cheap.

 

[Dr JIM]

Oh please Myth give me any reference that purity analytical data can be obtained using MS alone.

Tell me where in the heck did the numerical values used in the calculations come from? What source was used for these calculations, the AUG from a MS?

I mean Im only trying to find out how this type of purity data is supported in the analytical chemistry literature. If you have it lets see it bc I'll use it also, seriously!

The fact is EVERY ONE of those "scans" are a MS and NONE are CHROMATOGRAPHS EVEN THOUGH ONE IS LABELED AS SUCH! (The sloping appearance was obtained by slowly injecting the CHCL3 into a MS.)

So tell me how and why you or anyone else is capable of obtaining analytical data using some unpublished technique or procedure.

 

[brutus79]

He's an idiot!!!! Plain and simple, I won't even indulge him in discussing it either!!!!!

And the fact that I have lots of reports, he has none!!! Well he has a few but they are bullshit reports that have compounds you would NEVER see in AAS!!!! Want to waste your money, please send all you compounds to Jim for testing....

-Myth-

Really? Butthurt much or just invested in the lab somehow? Are you like invent help for ugl's?

 

[MythotiK]

Oh please Myth give me any reference analytical data that supports obtaining purity from an isolated MS.

How do your reports differ from mine other than yours have "OLIVE OIL" in them, I have that same report from YOU sitting in front of me at my desk as I type this...You were asked about it by your customer and the ONLY answer you have was,

"I don't know"!!!!!!!!!!!!

 

[One Pharma]

Quick note to everyone:

When i say vanilla reload, I mean the prepaid reload pak. This is NOT a plastic card that looks like a credit card. It is a single sheet like a loto ticket with a number you will scratch off the back that has 10 digits. I cannot do anything with the actual plastic card. I dont want to see people waste their money.

That is all

 

[Dr JIM]

How do your reports differ from mine other than yours have "OLIVE OIL" in them, I have that same report from YOU sitting in front of me at my desk as I type this...You were asked about it by your customer and the ONLY answer you have was,

"I don't know"!!!!!!!!!!!!

THAT'S THE BEST YOU GOT me telling someone the truth "I don't know"!

But rest assured the oil was not analyzed bc it's decanted using methanol which contains the desired AAS for study, and as a result it's irrelevant!

But what should be even more obvious is how ALL of the analytical PURITY assays you PERFORMED are bogus since they were ALL conducted using the same flawed procedure that must have come from outer space.

Tell me Myth what techniques are you using that NO ONE else is using, and that's NO ONE.

I mean have you EVER posted calibration data, sampling handling, reference standards, or enlighten ANYONE WHERE THE NUMERICAL VAUES CAME FROM SINCE THEY ARE USED TO DETERMINE "PURITY CALCULATION" nope!

Crap the holes in your analyses are so large I could drive a Mac truck thru any of them lol.

For the time being let's focus on ONLY ONE aspect of THIS MS. Tell me how the purity data was obtained?

 

[MythotiK]

THAT'S THE BEST YOU GOT me telling someone the truth "I don't know"!

But rest assured the oil was not analyzed bc it's decanted using methanol which contains the desired AAS for study, and as a result it's irrelevant!

But what should be even more obvious is how ALL of the analytical PURITY assays you PERFORMED are bogus since they were ALL conducted using the same flawed procedure that must have come from outer space.

Tell me Myth what techniques are you using that NO ONE else is using, and that's NO ONE.

I mean have you EVER posted calibration data, sampling handling, reference standards, or enlighten ANYONE WHERE THE NUMERICAL VAUES CAME FROM SINCE THEY ARE USED TO DETERMINE "PURITY CALCULATION" nope!

Crap the holes in your analyses are so large I could drive a Mac truck thru any of them lol.

For the time being let's focus on ONLY ONE aspect of THIS MS. Tell me how the purity data was obtained?

Nope, never have posted any of that!

 

[MythotiK]

One Pharma, I guess you should send your compounds to Jim, since all my tests are invalid...

 

[Dr JIM]

Fine Myth, but this "One Pharma" assay used the same erroneous technique to support the results!

I'd really like for someone to tell me how this is done bc I've probably shown this and several other MS or "spectrographs" to 5-6 chemists and every single one said the same thing, MS can NOT be used to derive quantitative information, unless it's used in tandem with some form of chromatography.

It's simply inherently flawed for a variety of reasons Myth such as there is no means of accounting for, those substances which don't ionize, those compounds which ionize yet are not detected, those factors that influence the extent a chemical ionization, the influence of fragmentation on ionization intensity, etc and probably most important the inability of this type of analysis to be corroborated and thereby confirmed by another lab.

 

[Dr JIM]

One Pharma, I guess you should send your compounds to Jim, since all my tests are invalid...

Again Myth I'm asking a legit question and if you can not answer how your purity data is obtained, people would be wise to assume it's flawed, IMO.

SIMPLE QUESTION really so why cant you answer? Look at the tests I've posted. You're correct Myth, there aren't many but It's all there for anyone to question, comment upon or criticize, such as the reference standards, calculations, AUG data, sample handling, solvents used, absorbance wavelengths.

Of course it's all there bc that's how these tests SHOULD BE DONE and anything less is misleading Meso members.

I will post six more tomorrow, yea it's slow sure (these SIX samples required 8 weeks plus) but I will not accept BS I can't understand, perhaps you should do the same.

 

[mercury]

MS can NOT be used to derive quantitative information, unless it's used in tandem with some form of chromatography.

it has to be calibrated with know standards with purity of 99.9%

I have all my test done on Agilent GC/MS 7010.

When you build method in ChemStation to run your sample you enter your calibration data and then It is automatic it just pops with data up after the run.

 

[Moose]

Dr Jim you haven't been in the underground much lately. We've missed you pal! Thanks for showing up.

 

[johnnyBALLZ]

Myth why are you acting like this? Just find out the answer to his question(s)..

Most of this MS/HPLC stuff is way over my head but even I understand Dr. Jim's point..

 

[MassTurk]

Myth I am one of many who have respect for you. I hope for a good answer like mercuries

 

[Dr JIM]

Shit Myth I understand how frustrating it can be when someone questions your/my analyses.

Few people have any idea how much time is involved in this process AND I don't even do the testing. But certain aspects of chemical assays must be revealed regardless of how annoying or time consuming some questions may be.

And there is no question revealing that information which explains how a primary outcome (qualitative or quantitative data) such as purity or concentration totals, were derived, especially when the listed procedure deviates from an accepted norm, is an accepted prerequisite which in this case should have been established or commented upon long ago, IMO