peptide shelf life after reconstitution.

[Stealth500]

Trying to decide if buying higher mg peptides is the right choice.

perhaps someone can chime as to which of these statements is correct and which is false.

"The bacteria found in air will degrade a peptide, which is why reconstituting a peptide with sterile water only makes sense if you intend on using the whole content of the vial within a day or two.

After 2-3 days, enough bacteria will have entered the vial to start to degrade the peptide, and a few days later it will be virtually useless (even if it has been stored in a fridge).

Peptides reconstituted in BAC water, however, can last for up to 2 weeks before starting to degrade and lose potency. After 4 weeks, the peptide will be pretty much useless (assuming it has been stored in a fridge).

Biggest Mistake People Make​

This is important because a lot of people think their 10,000iu HCG vial (or any other peptide for that matter) will last them 10 weeks if they pin 500iu twice a week, when the reality is they will only be able to take advantage of roughly 40% of that vial before it degrades almost completely.

Take this into account when using peptides, or you will continue to pin for no reason at all, and you will stop seeing the benefits you were expecting."

this next response was generated by chat GPT

" Reconstituted peptides stored in the refrigerator can remain stable for several days to a few weeks, but their stability can vary based on factors like the peptide's nature and storage conditions. For longer-term storage, it's advisable to aliquot the peptide and store it at lower temperatures, which can extend the shelf life for several months to a year or more."

 

[Stealth500]

"Yes, repeatedly freezing and thawing a reconstituted peptide can degrade its potency. It is recommended to aliquot the peptide into smaller portions to avoid multiple freeze-thaw cycles."

seems ordering a peptide that won't be used within 2 weeks is wasteful.

what is the point in ordering say 50mg vials of tirz?

 

[Ghoul]

There are so many variables regarding peptide longevity the only real answer is " reconstitute properly, use pharma BAC and get the smallest vial that's practical so it spends as little time reconstituted as possible."

 

[Stealth500]

so ordering a peptide such as tirz for long term use it would be advisable to order both smaller and mid potency vials?

 

[Ghoul]

Peptide is as broad a category as food. Some will remain stable for months, reconstituted, others will be significantly degraded within weeks.

For Tirz, I would advise a vial you would use within 30 days of reconstitution. What weekly dose (s) are you planning to use?

 

[Stealth500]

starting dosage is 2.5 so i think ordering one kit at 10mg and another at 30mg for longer term use would be sufficient.

 

[Stealth500]

Tirz, I would advise a vial you would use within 30 days of reconstitution. What weekly dose (s) are you planning to use?

thanks man.

 

[jaxked]

Kind of shocked by these answers myself. Why are the companies selling these massive bottles of peps if they're only good for 30 days once mixed?

 

[flipup7]

Kind of shocked by these answers myself. Why are the companies selling these massive bottles of peps if they're only good for 30 days once mixed?

The reason companies are selling them is because people are buying them.

But to the point, there are so many variables. It may be fine for a few months or it may only last a day or two depending on the peptide, storage conditions, bac water used, etc.

The main concern for most things is rate of degradation, its not like a peptide that would be fine at 29 days would be useless at 31 days. For the most part they will just become less effective with more time.

 

[jaxked]

The reason companies are selling them is because people are buying them.

But to the point, there are so many variables. It may be fine for a few months or it may only past a day or two depending on the peptide, storage conditions, bac water used, etc.

The main concern for most things is rate of degradation, its not like a peptide that would be fine at 29 days and would useless at 31 days. For the most part they will just become less effective with more time.

Ok, but for GLP's such as tirzepatide, sema, reta etc. How fast would they degrade in bac water in the fridge? I came on here looking for this info as there's not even any information on the chemical websites ie. Sigma alrich.

Had jamo conducted testing?

These tirzepatide bottles are getting into the 60mg range and the semaglutide 30mg.

I can see using a 40iu bottle of gh in 4 days and that being fine but a 30mg bottle of semaglutide? That's 12 weeks at 2.5mg a week.

 

[zpped]

The peptide testing group has jano results showing reconned tirz in the fridge only degrading 0.3% over 90 days. (and ~2% when stored in a closet)

It doesn't degrade that quickly. (It also withstands freezing at least once really well)

 

[flipup7]

Novo makes most Ozempic pens to have 4 doses, so that would be at least 28 days assuming it was produced right before being delivered. Thats of course with optimal pharma conditions.

I've seen compounding pharmacies that send vials of Tirz with 4 doses also. Thats probably a lot closer to the conditions any of us would see.

So that would be in line with Ghoul's 30 day rule of thumb. Given that, you'd only really want a 60mg tirz vial if you were at the max 15mg weekly dose.

 

[Stealth500]

i went down the rabbit hole again reading for a while after making this thread. One possibly reasonable solution to prolonging peptide shelf life would be pens. but imo.. just use the smallest vials to ensure potency-if you have to reconstitute an new vial each day so be it.


" Reconstituting a peptide and injecting it into an insulin pen can help with the delivery and potentially extend the shelf life of the peptide, but there are several important factors to consider:

1. Air Exposure: Peptides can degrade when exposed to air, as oxygen can lead to oxidation. Using an insulin pen, which is designed to minimize air exposure, may help reduce this risk.
2. Storage Conditions: Insulin pens are typically designed to maintain a stable environment for the medication. However, the specific storage conditions (temperature, light exposure) for the peptide should be considered, as some peptides require refrigeration.
3. Compatibility: Not all peptides are compatible with insulin pens. It's crucial to ensure that the peptide can be safely stored and delivered using the pen's mechanism. Some peptides may require specific formulations or diluents.
4. Stability: The stability of the reconstituted peptide in the pen should be evaluated. Some peptides may have a limited shelf life even when stored properly, so it's essential to follow guidelines for the specific peptide.
5. Consultation with Professionals: It's always best to consult with a healthcare professional or a pharmacist who has experience with peptides to ensure that the method of storage and delivery is appropriate and safe.

In summary, while using an insulin pen may help reduce air exposure and potentially extend the shelf life of a reconstituted peptide, it is essential to consider the specific characteristics of the peptide and consult with a professional for the best practices."

 

[Ghoul]

There is no comparison between pharma and UGL peptides.

Most pharma peptides have 3+ ingredients to prevent degradation, UGL's usually have jack shit. On top of that, PH, a MAJOR factor in the rate of degradation and carefully controlled in pharma products is random in UGL.

Then you've got stupidly high concentrations, like 20mg Sema, which speeds degradation even more.

No test on a UGL peptide can be applied to another vendor or batch. There are simply too many variables. It's not like carefully formulated pharma that's identical from batch to batch, without fail.

All you can do is control the factors we know help preserve all peptides, and try to use a vial up as quickly as possible after reconstitution.

It's not always possible. I can't use up a vial of MT-II quickly, but with a cryofreezer I may start to "aliquot" half of it, which is what labs do with portions of reconstituted peptide that won't be used for a while, rather than keep it in the refrigerator for months.

 

[Stealth500]

use up a vial of MT-II quickly

im suprised you would use mt2. with all the reading you do.. are you not concerned about some of the potential sides? some as serious as renal infarction.

 

[Ghoul]

im suprised you would use mt2. with all the reading you do.. are you not concerned about some of the potential sides? some as serious as renal infarction.

Funny you mention that, as I was just reading one of the low single digit cases of that reported in the med literature.

Those were enormous doses. multiple 7mg+. Insanity.

Typical for me would be .50-.75mg.

I find it to be a potent "anti-aging" compound in certain effects rarely discussed outside of obscure research papers.

I haven't found any evidence of harm at conventional doses, though I'm aware there's always some degree of risk, MT-II has been in use for quite a while. Many have used it for over a decade, and there don't seem to be any widespread health issues as a result, Nor has any of the research turned up any troubling concerns (also using reasonable doses).

The time and number of users implies a certain degree of safety. ("patient years" of experience).

I'd be more concerned with any of the novel peptides that have no track record behind them that many seem willing to immediately start using.

As they say, the dose makes the poison.

 

[Stealth500]

Funny you mention that, as I was just reading one of the low single digit cases of that reported in the med literature.

Those were enormous doses. multiple 7mg+. Insanity.

Typical for me would be .50-.75mg.

I find it to be a potent "anti-aging" compound in certain effects rarely discussed outside of obscure research papers.

I haven't found any evidence of harm at conventional doses, though I'm aware there's always some degree of risk, MT-II has been in use for quite a while. Many have used it for over a decade, and there don't seem to be any widespread health issues as a result, Nor has any of the research turned up any troubling concerns (also using reasonable doses).

The time and number of users implies a certain degree of safety. ("patient years" of experience).

I'd be more concerned with any of the novel peptides that have no track record behind them that many seem willing to immediately start using.

As they say, the dose makes the poison.

just seems to me there hasn't been enough research on it. My concern is this sort of strange stimulation it causes in the brain having weird adverse effects like erections. As for the dosage on the cases i've read with renal infarction it didn't see anything egregious but i can't recall the dosage at all.

also renal infarction is commonly misdiagnosed. and if a person were using aas along with the mt2 a doctor may attribute kidney issues with aas. or perhaps the patient may forget to mention the mt2 altogether when using other drugs.

 

[Stealth500]

just seems to me there hasn't been enough research on it. My concern is this sort of strange stimulation it causes in the brain having weird adverse effects like erections. As for the dosage on the cases i've read with renal infarction it didn't see anything egregious but i can't recall the dosage at all.

i also didnt know it had anti aging effects..

 

[Ghoul]

just seems to me there hasn't been enough research on it. My concern is this sort of strange stimulation it causes in the brain having weird adverse effects like erections. As for the dosage on the cases i've read with renal infarction it didn't see anything egregious but i can't recall the dosage at all.

also renal infarction is commonly misdiagnosed. and if a person were using aas along with the mt2 a doctor may attribute kidney issues with aas. or perhaps the patient may forget to mention the mt2 altogether when using other drugs.

30 years, hundreds of studies stretching back into the 1990's, I'm not sure how much more you can expect or how many non-pharma peptides have a more substantial record of research behind them. Which peptides do you find meet your standard of acceptable research to be confident in their relative safety?

I don't see the brain effects to be adverse in any way. To the contrary, it's precisely these effects that in total, induce changes that make the aging brain revert to a more youthful state. Restoring reactions and unconscious behaviors, broadly observed in young mammals, across species, that diminish over time.

 

[Stealth500]

i've seen you say you have a preference for tesamorelin over ipamorelin because of it's research and long term use and fda approval ect. just seems odd a product like mt2 that is not among the most extensively researched peptides (i don't know where your getting this) compared to others in the field. Its clinical development has ceased, and it is primarily available as an unregulated product online.

Melanotan II (MT2) primarily activates melanocortin receptors in the brain, particularly the MC4 receptor, which is associated with sexual arousal and erections. It may also involve the MC3 receptor,(excessive activation of certain receptors can potentially lead to various health issues)
but the MC4 receptor is the most documented in relation to its erection-enhancing effects.
Overstimulation of the melanocortin 4 receptor (MC4R) can lead to increased appetite and weight gain, as well as potential metabolic abnormalities. This is particularly relevant in the context of obesity and related health issues.