Qingdao Sigma Chemical Co., Ltd (International, US, EU, Canada and Australia domestic

That's an excellent question. From what I've found a typical 25mm syringe filter has a .05ml hold up volume.

13mm has a .01ml hold up volume.

Also important to ensure the filter uses a "low protein adhesion" membrane.

Finally, doing an "air purge" into the vial will push most retained liquid out of the filter.
Those numbers you're listing are after an air purge. Hold up is much higher than that before an air purge.
 
Back
Top