Ghoul
Well-known Member
For those interested in wasting money by following the lead of evil big pharma and protein researchers by needlessly filtering aggregates from their peptides, here are the specs you need to find the correct ones.
In theory, filtering into your syringe just prior to administration is ideal, but even drawing and putting back into the original vial will help. Large aggregates take time, seemingly days at least, to grow from dimer or contaminating silicone droplets from what I've read. You're also eliminating sediments from the peptide excipient that may not have dissolved:
-Material: PES
-Sterile
-Your preferred connections. Most are luer lock to slip tip. I prefer luer to luer using a vial spike to speed things up vs a needle.
-Hydrophilic
-NON-Charged (if it doesn't specify that, it should say "low protein adhesion", "low protein loss", or "low protein adsorption".)
-.2 or .22um (or even .1um will allow peptides through and eliminate more aggregates, but really slow).
-Some recommend 4mm to minimize loss of liquid in the filter. I tried this and what I assume was "protein fouling" completely clogged it before 1ml got through. A good sign it's working. but not practical imo. 13mm is a good trade off.
You can dilute your peptide a little more than usual to compensate, so the retained liquid won't cause as much a loss of active peptide.
Pushing a syringe of air through the filter at the end will eject more liquid from the filter.
Expect a price between 40¢ and $3 depending on source and quantity.
If you need more detailed info regarding peptide filtration, links to current studies, or just want to chat about state of the art protein aggregation mitigation methods contact @BuildABro or @nightprowler7
(note: please send pic first to prove "you even lift" and wait for response)
In theory, filtering into your syringe just prior to administration is ideal, but even drawing and putting back into the original vial will help. Large aggregates take time, seemingly days at least, to grow from dimer or contaminating silicone droplets from what I've read. You're also eliminating sediments from the peptide excipient that may not have dissolved:
-Material: PES
-Sterile
-Your preferred connections. Most are luer lock to slip tip. I prefer luer to luer using a vial spike to speed things up vs a needle.
-Hydrophilic
-NON-Charged (if it doesn't specify that, it should say "low protein adhesion", "low protein loss", or "low protein adsorption".)
-.2 or .22um (or even .1um will allow peptides through and eliminate more aggregates, but really slow).
-Some recommend 4mm to minimize loss of liquid in the filter. I tried this and what I assume was "protein fouling" completely clogged it before 1ml got through. A good sign it's working. but not practical imo. 13mm is a good trade off.
You can dilute your peptide a little more than usual to compensate, so the retained liquid won't cause as much a loss of active peptide.
Pushing a syringe of air through the filter at the end will eject more liquid from the filter.
Expect a price between 40¢ and $3 depending on source and quantity.
If you need more detailed info regarding peptide filtration, links to current studies, or just want to chat about state of the art protein aggregation mitigation methods contact @BuildABro or @nightprowler7
(note: please send pic first to prove "you even lift" and wait for response)
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