Qingdao Sigma Chemicals
Subscriber
No link, purity is almost same for all versions: 10IU, 15IU, 24IU and 36IU
MESO-Rx
Anabolic Steroids
QSC - HGH 36IU Purple Top - Jano labtest
- Thread starter Sampei
- Start date
That's a new HGH batch just made. It will stabilize at a lower purity tho, can't stay like that for long, it will start to have dimer with time too but very small amount as shown by my latest test of an HGH batch that was running around for a few months at it was at the end mostly.
That's how Tracy explained to me last time I asked.
Of course there are batch of HGH of other manufacturer that don't even reach 98+% purity even when freshly made.
readalot
Member
This is an outstanding thread Sampei. You have truly outdone yourself. Bless you.
readalot
Member
Now I am going to have to head to head the pharma zomacton vs the high quality ugl products. G Damn it LOL.
MyNameIsJeff
Well-known Member
Interesting. So the fact that the more recent samples of your GH, sent in by customers, have comparatively 'low' purity of 96-97% is due to degradation during transit from you to the customer, and from the customer to Jano?
In that case, it could be nice to experiment with other/ additional excipients, such as phosphoric acid, sodium hydroxide, glycine, monobasic sodium phosphate, dibasic sodium phosphate.
MyNameIsJeff
Well-known Member
The following is from ChatGPT, so take it with a big grain of salt:
The addition of buffers to a lyophilized powder formulation can indeed increase the stability of peptides like HGH prior to reconstitution, but their role in this context is a bit different compared to when they are dissolved in solution.
How Buffers Impact Lyophilized Formulations:
In a lyophilized (freeze-dried) state, buffers do not function in the same way they do in solution (i.e., actively maintaining pH through equilibrium between proton donors and acceptors). However, they can still provide stabilizing effects during the freeze-drying process and storage before reconstitution, mainly by protecting the peptide from pH-related degradation mechanisms. Here's how:
1.
Buffers can stabilize pH-sensitive peptides like HGH during the freeze-drying process. Even in the frozen or dry state, peptides can experience changes in their microenvironment due to local variations in pH that occur during the freezing and drying stages.
Buffers like phosphate or histidine help ensure that the environment surrounding the peptide remains within a stable pH range during these critical phases, preventing degradation mechanisms like deamidation (which can still occur at low moisture levels).
2.
Residual moisture is almost always present in lyophilized products, even after drying. This residual moisture can facilitate minor reactions, including pH shifts, in the microenvironment around the peptide.
Buffers help to minimize pH fluctuations in the lyophilized state by stabilizing the residual water that remains, thus reducing the risk of pH-related degradation before the product is reconstituted.
3.
Peptides like HGH are prone to aggregation during freeze-drying and storage. Buffers can help by preventing significant changes in the peptide's charge state (which is pH-dependent), thus reducing the risk of aggregation or denaturation in the dry state.
4.
Certain chemical degradation processes like deamidation or oxidation can still occur in lyophilized peptides, especially if the residual moisture level is high. Buffers, even in their solid state, can mitigate these reactions by maintaining the peptide’s microenvironment within a favorable pH range.
Summary:
Buffers in the lyophilized powder help stabilize the microenvironment of the peptide during both the freeze-drying process and storage. Even though the pH buffering action isn't actively happening (since the system isn't aqueous), the presence of a solid-state buffer can prevent damaging pH shifts and help maintain the structural integrity of the peptide.
For HGH stability, a buffer like phosphate or histidine could be critical, especially in formulations with excipients like mannitol that don’t inherently control pH. The goal is to protect HGH from pH-related degradation prior to reconstitution.
"Phosphate Buffer: A mix of monobasic sodium phosphate and dibasic sodium phosphate (commonly used to maintain a pH in the range of 6.0-7.0)."
The addition of buffers to a lyophilized powder formulation can indeed increase the stability of peptides like HGH prior to reconstitution, but their role in this context is a bit different compared to when they are dissolved in solution.
How Buffers Impact Lyophilized Formulations:
In a lyophilized (freeze-dried) state, buffers do not function in the same way they do in solution (i.e., actively maintaining pH through equilibrium between proton donors and acceptors). However, they can still provide stabilizing effects during the freeze-drying process and storage before reconstitution, mainly by protecting the peptide from pH-related degradation mechanisms. Here's how:
1.
Buffers can stabilize pH-sensitive peptides like HGH during the freeze-drying process. Even in the frozen or dry state, peptides can experience changes in their microenvironment due to local variations in pH that occur during the freezing and drying stages.
Buffers like phosphate or histidine help ensure that the environment surrounding the peptide remains within a stable pH range during these critical phases, preventing degradation mechanisms like deamidation (which can still occur at low moisture levels).
2.
Residual moisture is almost always present in lyophilized products, even after drying. This residual moisture can facilitate minor reactions, including pH shifts, in the microenvironment around the peptide.
Buffers help to minimize pH fluctuations in the lyophilized state by stabilizing the residual water that remains, thus reducing the risk of pH-related degradation before the product is reconstituted.
3.
Peptides like HGH are prone to aggregation during freeze-drying and storage. Buffers can help by preventing significant changes in the peptide's charge state (which is pH-dependent), thus reducing the risk of aggregation or denaturation in the dry state.
4.
Certain chemical degradation processes like deamidation or oxidation can still occur in lyophilized peptides, especially if the residual moisture level is high. Buffers, even in their solid state, can mitigate these reactions by maintaining the peptide’s microenvironment within a favorable pH range.
Summary:
Buffers in the lyophilized powder help stabilize the microenvironment of the peptide during both the freeze-drying process and storage. Even though the pH buffering action isn't actively happening (since the system isn't aqueous), the presence of a solid-state buffer can prevent damaging pH shifts and help maintain the structural integrity of the peptide.
For HGH stability, a buffer like phosphate or histidine could be critical, especially in formulations with excipients like mannitol that don’t inherently control pH. The goal is to protect HGH from pH-related degradation prior to reconstitution.
"Phosphate Buffer: A mix of monobasic sodium phosphate and dibasic sodium phosphate (commonly used to maintain a pH in the range of 6.0-7.0)."
Last edited:
egruberman
Well-known Member
I suspect most of this is counterfeit and even if it's not, most I've seen tests out in a similar range for purity.
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