As many know, hCG is an important part of treatment. Is it safe to freeze reconstituted hCG for later use.
MESO-Rx
Anabolic Steroids
Should hCG be kept frozen for later use?
- Thread starter Michael Scally MD
- Start date
Iatola
New Member
Good to know. Thanks Doc
Iatola
New Member
I initially misread that as a statement, the product of a quick read on an iPhone. Sorry Doc.
This would be interesting to know, especially with higher concentration vials and a low dose schedule. You could potentially preload and freeze.
This would be interesting to know, especially with higher concentration vials and a low dose schedule. You could potentially preload and freeze.
I remember a while back people would do that with their IGF. Don't know if it would work with hcg. I'll keep checking in cause I would like to know the answer as well
Take the Poll!!!
It is just a click. And, possibly very important.
It is just a click. And, possibly very important.
It would be great if it could!
I don't believe it's recommended for stability reasons. BUT, the question Dr. Scally has presented it possibly could be done with success. Do I know how...? NOPE!!!
mands
mands
As a corollary question, would you freeze reconstituted hGH for later use?
Take The Poll!
Take The Poll!
Pericles
New Member
It is my understanding, and correct me if I am wrong, but freezing destroys reconstituted HCG.
I made the mistake of putting it in the freezer a couple of times.
The HCG I get from a compounding pharmacy says "do not freeze."
I made the mistake of putting it in the freezer a couple of times.
The HCG I get from a compounding pharmacy says "do not freeze."
Couldn't we answer this by freezing some, letting out the then treating it on a pregnancy test? If I had any left I'd do that right now to see.
No! In fact, even doing a specific quantitative assay would not be adequate. Do you know why?
How big and complex is hCG v hGH?
NUTROPIN (somatropin)
NUTROPIN (SOMATROPIN) KIT [GENENTECH, INC.]
Nutropin® [somatropin (rDNA origin) for injection] is a human growth hormone (hGH) produced by recombinant DNA technology. Nutropin has 191 amino acid residues and a molecular weight of 22,125 daltons. The amino acid sequence of the product is identical to that of pituitary derived human growth hormone.
STABILITY AND STORAGE
Before Reconstitution—Nutropin and Bacteriostatic Water for Injection, USP (benzyl alcohol preserved), must be stored at 2–8°C/36–46°F (under refrigeration). Avoid freezing the vials of Nutropin and Bacteriostatic Water for Injection, USP (benzyl alcohol preserved). Expiration dates are stated on the labels.
After Reconstitution—Vial contents are stable for 14 days when reconstituted with Bacteriostatic Water for Injection, USP (benzyl alcohol preserved), and stored at 2–8°C/36–46°F (under refrigeration). Avoid freezing the reconstituted vial of Nutropin and the Bacteriostatic Water for Injection, USP (benzyl alcohol preserved).
I have no idea, but want to learn.
An assay will show that which comes up within their limits, but not necessarily that which is bioactive. In order to see whether freezing is not harmful there would need to be clinical trials to test for bioactivity, serum testosterone levels. It would be easy to have immunologically active hCG that is NOT biologically active.
Last edited:
Pericles
New Member
Or, I could just read the big fat sticker from my pharmacy that says "DO NOT FREEZE"[
)]
What about a very similar drug (LH)! This should put to rest the notion that one can safely freeze hCG for later use. If you do, it is based upon NO evidence, contrary evidence, and at your own risk (and $$$).
MENOPUR (follitropin and luteinizing hormone)
MENOPUR (FOLLITROPIN AND LUTEINIZING HORMONE) KIT [FERRING PHARMACEUTICALS INC.]
How should I store Menopur®? Store Menopur® in the refrigerator at 36º to 46ºF (2º to 8ºC), or at room temperature, 68º to 77º F (20º to 25ºC).
DO NOT FREEZE. [Emphasis in CAPS mine]
MENOPUR (follitropin and luteinizing hormone)
MENOPUR (FOLLITROPIN AND LUTEINIZING HORMONE) KIT [FERRING PHARMACEUTICALS INC.]
How should I store Menopur®? Store Menopur® in the refrigerator at 36º to 46ºF (2º to 8ºC), or at room temperature, 68º to 77º F (20º to 25ºC).
DO NOT FREEZE. [Emphasis in CAPS mine]
Prior to this poll, I was my belief that hCG could NOT be frozen - just based on the package insert. When you started the poll, I started to second guess that and did some searching. All the manufacturers data I looked at, as well as any pharmaceutical research I found, warned against freezing reconstituted hCG. I was unable to find any evidence showing freezing is acceptable..
This is an important topic because it's generally accepted in the bro lore that freezing reconstituted hCG is okay. I looked at the bro lore to see if this was based on anything more than the usual personal opinion and anecdotes. Interestingly, I found much of the belief seems to stem from comments made by this Crisler character that was mentioned in the other thread. He claims to have been convinced by the data from some compounding pharmacy but the data cannot be published because it's "proprietary"... Un huh.
Given the less than stellar track record of most compounding pharmacies, I would be very apprehensive about taking their word over the big pharmaceutical companies with billions of dollars for R&D. Especially because their data is supposedly proprietary.
Interesting topic.
CBS
Last edited:
Storage/Stability
Dilute aqueous solutions undergo rapid loss of activity
when stored frozen, or heated, or if excess acid or
base is added. Gelatin and serum proteins help to
stabilize aqueous solutions of hCG. hCG is stable in a
glycerol solution at 100°C for one hour.
http://http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/c1063pis.Par.0001.File.tmp/c1063pis.pdf
Dilute aqueous solutions undergo rapid loss of activity
when stored frozen, or heated, or if excess acid or
base is added. Gelatin and serum proteins help to
stabilize aqueous solutions of hCG. hCG is stable in a
glycerol solution at 100°C for one hour.
http://http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/c1063pis.Par.0001.File.tmp/c1063pis.pdf
I think the evidence is not there to freeze hCG for later use without running the risk of decreasing effectiveness. Why take the chance for any decreased efficacy, especially since it is your BALLS.
It would be nice (and easy) to test what freezing would do to hCG bioactivity. And, the end point must be bioactivity and NOT immunoactivity. As it would be a simple clinical trial, maybe someone will take the time to run.
It would be nice (and easy) to test what freezing would do to hCG bioactivity. And, the end point must be bioactivity and NOT immunoactivity. As it would be a simple clinical trial, maybe someone will take the time to run.
Similar threads
Sponsors
Latest posts
-
Sigma Audley Inc. - Your source for peptides, ancillaries, AAS, and more!- Latest: SigmaAudley China Peptide
-
-
-
-
