Sterilising vials the pedantic way

[29trt]

Sterile liquid in NON-sterile vials results in non-sterile liquid, BA is not going to make it sterile.
and you cannot compare dirty vials with puncturing a disinfected vial top with a sterile needle
You are either a troll or mentally challenged, either way you are on my ignore list.

 

[klokke23]

Speak for yourself; im here to learn.

Weird. Your entire post history is on source threads. You wouldn't be lying on the internet would you?

 

[TestTrenPrimo]

Weird. Your entire post history is on source threads. You wouldn't be lying on the internet would you?

The whole entire thing huh? I’m not even gonna argue with you here because you obviously didn’t scroll very far lol

 

[OffendHand]

hey man awesome setup, do you find the vials carry alot of condensated water after the autoclave?

I can't speak for anyone else, but I would turn an oven on to low heat (>125F) and put the sterilized glassware (still in the sealed bags) in the oven until everything was dry. That avoided any issues with condensation in the finished oil.

what on earth are you doing. you do not need to sterilize ANYTHING in order to prepare a safe injectable product. as in nothing you do has to be sterile from start to finish. you can either research this for yourself and get third party accounts or even better just do it for yourself and it will be self evident. first hand knowledge is the best knowledge. actually its the only knowledge.

I am curious if you can explain to mean why you think you need to autoclave anything when preparing injectable steroids.

It minimizes your potential infection vectors. We can't realistically control everything in our homes in the way that you can in a lab. Most people don't ahve the means to buy or the ability to effectively use a laminar flow cabinet, but they can make sure that their glassware is as clean as possible to start. So you do what you realistically can. We can't build FDA-certified manufacturing facilities in our spare bedrooms. But we can make sure that we're doing basic sterilization, and going as far as we can.

The second that a tattoo artist opens their tubes and needles, they're no longer sterile. But your odds of getting a nasty infection from an artist that autoclaves their needles and tubes is far, far lower than it is if you get a tattoo from someone that uses them directly from the factory without sterilization first.

 

[Cdtoth666]

I can't speak for anyone else, but I would turn an oven on to low heat (>125F) and put the sterilized glassware (still in the sealed bags) in the oven until everything was dry. That avoided any issues with condensation in the finished oil.

It minimizes your potential infection vectors. We can't realistically control everything in our homes in the way that you can in a lab. Most people don't ahve the means to buy or the ability to effectively use a laminar flow cabinet, but they can make sure that their glassware is as clean as possible to start. So you do what you realistically can. We can't build FDA-certified manufacturing facilities in our spare bedrooms. But we can make sure that we're doing basic sterilization, and going as far as we can.

The second that a tattoo artist opens their tubes and needles, they're no longer sterile. But your odds of getting a nasty infection from an artist that autoclaves their needles and tubes is far, far lower than it is if you get a tattoo from someone that uses them directly from the factory without sterilization first.

Completely understand your point BUT what I'm trying to explain is that all of those other "sterilization" procedures will NOT reduce your risk of infection any more than just filtering and using Benzyl alcohol. This is backed by the principles of microbiology.

We mentioned mushroom cultivation, and believe it or not you need to take more sterilization precautions for indoor mushroom cultivation than you do making injectable steroids, all other things being equal. I mean, I do not know if you have any intravenous drug experience, but I know many people who mainline meth without almost any sterile precautions and have never had an infection. There is a reason for this.

 

[Cdtoth666]

Sterile liquid in NON-sterile vials results in non-sterile liquid, BA is not going to make it sterile.
and you cannot compare dirty vials with puncturing a disinfected vial top with a sterile needle
You are either a troll or mentally challenged, either way you are on my ignore list.

I don't understand what you are saying. it doesn't have to be sterile. i'M NOT sure what do you not get. and a disinfected vial top... I don't understand why the makes any difference. could you please explain to me what bacteria or viruses are going to be able to propagate and infest a glass vial or a butyl stopper? and then survive in an anaerobic bacteriostatic solution inside the vial long enough to infect you? I don't understand

 

[clearheaded]

" I know many people who mainline meth without almost any sterile precautions and have never had an infection. There is a reason for this."

explains all we need to know, about your brain power.

"principles of microbiology." you have never opened a textbook never mind ever have worked in a laboratory. its very obvious.

bacteria and virus can survive space...take care!

 

[Henryx]

Completely understand your point BUT what I'm trying to explain is that all of those other "sterilization" procedures will NOT reduce your risk of infection any more than just filtering and using Benzyl alcohol. This is backed by the principles of microbiology.

We mentioned mushroom cultivation, and believe it or not you need to take more sterilization precautions for indoor mushroom cultivation than you do making injectable steroids, all other things being equal. I mean, I do not know if you have any intravenous drug experience, but I know many people who mainline meth without almost any sterile precautions and have never had an infection. There is a reason for this.

When under extreme conditions, some bacteria will form endospores to protect themselves and become "deactivated". Benzyl alcohol is a bacteriostatic agent, which "stops bacteria from reproducing, while not necessarily killing them otherwise". Without "sterilization" (not disinfection), endospores may still be able to reactivate when the correct conditions are met (body temperature) and cause infection. Certain bacteria are highly resistant to common disinfection procedures and can be deadly if allowed to grow, e.g. Clostridium botulinum, Clostridium tetani, Geobacillus stearothermophilus.

Also, besides sterilization, depyrogenation is also very important in producing anything you gonna inject into your body and should be done simultaneously with sterilization. Pyrogens are the main cause of people having fever/flu-like symptoms because it is also resistant to a lot of chemicals like IPA. In order to remove pyrogens, prepare your bottles with a high heat (constant 260 °C for two hours, without preheating) or bleach or hydrogen peroxide (both need high conc.).

This is simply a precaution to minimize the risk for the sake of one's health. It is all a matter of probability, but you are the one who will decide whether to take the risk with your own body.

 

[Cdtoth666]

When under extreme conditions, some bacteria will form endospores to protect themselves and become "deactivated". Benzyl alcohol is a bacteriostatic agent, which "stops bacteria from reproducing, while not necessarily killing them otherwise". Without "sterilization" (not disinfection), endospores may still be able to reactivate when the correct conditions are met (body temperature) and cause infection. Certain bacteria are highly resistant to common disinfection procedures and can be deadly if allowed to grow, e.g. Clostridium botulinum, Clostridium tetani, Geobacillus stearothermophilus.

Also, besides sterilization, depyrogenation is also very important in producing anything you gonna inject into your body and should be done simultaneously with sterilization. Pyrogens are the main cause of people having fever/flu-like symptoms because it is also resistant to a lot of chemicals like IPA. In order to remove pyrogens, prepare your bottles with a high heat (constant 260 °C for two hours, without preheating) or bleach or hydrogen peroxide (both need high conc.).

This is simply a precaution to minimize the risk for the sake of one's health. It is all a matter of probability, but you are the one who will decide whether to take the risk with your own body.

No.

I have exhausted this topic, otherwise I wouldn’t be so confident. Botulism for example needs water the same as all those other things you mentioned. It is not something that you need to worry about. It will never be a problem…

Are y’all like type of people who sprayed their fruit during covid?

 

[mass gain]

I don't know if this is in here yet but it seems to be warranted so:

 

[Drugsarefun]

hey man awesome setup, do you find the vials carry alot of condensated water after the autoclave?

I was surprised to find zero condensation after the process, I've been using this method for awhile and had had zero issues with condensation ruining the brew.

 

[Luka.Halotestin]

Once everything has cooled down you can manouver the rubber stoppers onto the vials through the plastic without opening them, they should stay sterile in the packet for upto a year.

Hey bro, could you tell me why not just putting them together before steam-sterilizing? I find it easier than trying to assemble them while in plastic bag but if there is a reason for not doing it that way I want to hear it. I don't wanna make even the slightest mistake while doing this.

 

[Henryx]

Hey bro, could you tell me why not just putting them together before steam-sterilizing? I find it easier than trying to assemble them while in plastic bag but if there is a reason for not doing it that way I want to hear it. I don't wanna make even the slightest mistake while doing this.

You should never have any sealed (bottles/glassware/jugs etc.) in your autoclave. Pressure differences of more than one atm in the bottle versus the autoclave will make them explode. Also, the steam will not reach the bottle inside, resulting in inadequate sterilization.

 

[Cdtoth666]

You should never have any sealed (bottles/glassware/jugs etc.) in your autoclave. Pressure differences of more than one atm in the bottle versus the autoclave will make them explode. Also, the steam will not reach the bottle inside, resulting in inadequate sterilization.

Lol what are you doing is this a joke? Omg is This all just a joke cuz wow you guys got me so good. That’s right you have to use your “autoclave” I know what that means

 

[Henryx]

Lol what are you doing is this a joke? Omg is This all just a joke cuz wow you guys got me so good. That’s right you have to use your “autoclave” I know what that means

I don't quite understand what you mean. My last reply was trying to answer his question, not intending to make anyone awkward.

 

[clearheaded]

it means in in autoclave or pressure cooker. dont reply to him he is just a troll..

 

[longstad7282]

Ok so as a carry over from a previous thread I thought I'd share my technique for sterilising vials.

I see alot of practices that claim sterile but are far from sterile, if you want actual sterile vials this is one way to achieve that, although I have yet to swab my vials and put it on agar but I trust the process.

I pulled alot of this from growing mushrooms, adapted and used equipment I had on hand, it's much the same process but you have to be alot more sterile with mushroom growing than you do bottling gear.

Clean your vials as per lab glass cleaning instructions which you can find online:

Wash and rinse both vials and rubber stoppers in tap water and dish soap then rinse 3 - 4 times in distilled water, place in the oven to completely dry, usually 20 mins or so, wait for them to cool to the touch.

Place them into autoclave bags with the rubber stopper just above the vial, do not seal the vials, it helps if you have self sealing bags but the non sealing ones are cheaper and painters tape is fine to seal them with.

Place the packets into a PP5 bucket, this is important, it must be PP5, it'll have a recyclable symbol with a 5 in the centre, these can with stand the heat of a pressure cooker, everything else will melt, the one I have is 5 litres, cut a hole in the lid of the bucket and stuff with poly fill, again must be poly fill as that handles the heat, the hole stuffed with polyfill allows pressure relief while reducing the moisture inside the bucket, your bucket will most likely implode without it.

Cover the top with tinfoil to protect any water drops dripping onto the polyfill.

Place into the pressure cooker with 2.5 quarts of water at the bottom.
Turn on high and allow steam to escape for 10mins, place the weight on and bring the pressure up to a minimum 15psi, turn it down and hold it steady, I run mine at 18psi.
20mins is probably fine at 15psi but I like to run it for 45 just to be sure.

When it's cooled down you can remove the bucket, it's contents will be sterile untill you open it, so could sit on a shelf for months and be completely sterile inside.

Once everything has cooled down you can manouver the rubber stoppers onto the vials through the plastic without opening them, they should stay sterile in the packet for upto a year.

You can also fill them while still in the packet if using a syringe filter, just peirce right through the plastic and rubber stopper and fill them up, once full remove them from the packet and cap them.

If using a bottle top filter you'd need a flow hood or at a minimum a still air box to remain sterile while filling.

Question I am a little confused with the wash and rinse part when you wash the vials do you do it by hand or with a sponge or something because I am planning on doing your method seems really good just need to know that part thanks

 

[psauce]

Question I am a little confused with the wash and rinse part when you wash the vials do you do it by hand or with a sponge or something because I am planning on doing your method seems really good just need to know that part thanks

I'm not the person you're asking, but I can explain how it is done in a laboratory setting.

First, you prepare a soapy water bath and place all your vials in there. You can use a bottle brush or even a twisted up paper towel to make sure there's no particulate material or a chemical coating from the manufacturing process. After this, you rinse with normal running water, both to remove any soap residue and anything else. Using distilled water at this point is overkill.

Next, for the distilled water rinses, you want to fill the interior of the vial with the distilled water. The first time or two, it is not a bad idea to put a GLOVED thumb over the vial mouth and shake. This agitation helps get any leftover stuff from adhering to the side of the vial, even though the contact with your thumb isn't perfectly clean. The next couple of rinses, you don't want to touch the mouth of the vial at all, so that any thing that was on that small bit of your glove surface is washed out. You just fill the vial, dump the vial.

After the final distilled water rise, place the vial upside down on top of a piece of baked aluminum foil. This will prevent anything dust from falling into the mouth. (Just get some cheap, store brand foil with no coating, remove it from the roll, and bake it at 500 for an hour.)

This type of cleaning protocol is not only good for microbiology, but also for glassware to be used in chemical analysis of organics. So we're not talking about whole cells fucking up the analysis, we're talking about the oils from human skin wrecking everything.

 

[psauce]

There is a middle ground between a basic pressure cooker and a benchtop autoclave, which is the new crop of programmable pressure cookers. Even the cheapest and smallest autoclaves are insanely expensive, and they don't provide any sterilization that's better than a Ninja that you can order from Amazon. These are very quickly populating academic labs.

If someone doesn't want to sterilize their glassware, cool. BA will arrest growth, the water activity within an oil is low, and so you're unlikely to proliferate all that much. However, the literature is replete with instance of oils that contain vegetative pathogens and spores that would be happy to start growing when placed an inch inside of a warm, watery, nutrient rich muscle belly.

But hey, you do you.

 

[longstad7282]

I'm not the person you're asking, but I can explain how it is done in a laboratory setting.

First, you prepare a soapy water bath and place all your vials in there. You can use a bottle brush or even a twisted up paper towel to make sure there's no particulate material or a chemical coating from the manufacturing process. After this, you rinse with normal running water, both to remove any soap residue and anything else. Using distilled water at this point is overkill.

Next, for the distilled water rinses, you want to fill the interior of the vial with the distilled water. The first time or two, it is not a bad idea to put a GLOVED thumb over the vial mouth and shake. This agitation helps get any leftover stuff from adhering to the side of the vial, even though the contact with your thumb isn't perfectly clean. The next couple of rinses, you don't want to touch the mouth of the vial at all, so that any thing that was on that small bit of your glove surface is washed out. You just fill the vial, dump the vial.

After the final distilled water rise, place the vial upside down on top of a piece of baked aluminum foil. This will prevent anything dust from falling into the mouth. (Just get some cheap, store brand foil with no coating, remove it from the roll, and bake it at 500 for an hour.)

This type of cleaning protocol is not only good for microbiology, but also for glassware to be used in chemical analysis of organics. So we're not talking about whole cells fucking up the analysis, we're talking about the oils from human skin wrecking everything.

Thanks man very helpful