Storage conditions and dimer

[Ghoul]

Yes, as I wrote, I'm from the EU.. So, does 0.9% saline solution protect against bacteria for a longer period of time?

Saline does nothing to stop the growth of bacteria.

@Sampei knows where to get the supplies you need to make your own Bacteriostatic water in the EU, and the best technique. It's cheap and straightforward.

 

[Eddie.]

Without a bacteriostatic component, it's not safe to use for more than 24 hours after reconstitution. Jano's found a fairly high percentage of peptides failed sterility tests, not unexpected, and that bacteria feeds on protein. It's like you're reconstituting powdered eggs. You could add Benzyl Alcohol in the correct proportion. I assume you're in the EU and can't source BAC water?

So, 24 hours is considered to be ok? I've left with a few hospira bac bottles that going to end soon and the only EU site provided them is now closed..

If sterile water for injection is ok up to 24h, that means i could use half vial HGH a day (as i'm currently using) with sterile water. Right?

 

[Ghoul]

I hear it for the first time... that doesn't sound good

TLDR: The presence of NaCL (salt), destabilizes HGH.

 

[hellogre2]

Control what you can, what's done is done. If you're keeping it, the sooner you get it in the refrigerator, the less degradation it'll experience. It would take extensive testing of that specific batch to know how fast it degenerates, but we know whatever it is, it's *less* in cold temps.

Also, reconstitute to 3ml. When HGH is damaged, and it starts to aggregate, experiments show the greater the concentration, the worse the aggregation is because, simply put, the molecules are crowded and bump into each other more, increasing the chance of sticking to each other.

Here's an illustration of how damaged (oxidized in this case) HGH forms more and larger aggregates with increased concentration:

View attachment 322703

For now, it's 1 ml. Last week, I ordered enough pharma gade raw materials to make my own BAC for the next 10 decades. Living in Europe, having premade pharma grade is a nightmare.

 

[Bobik]

For now, it's 1 ml. Last week, I ordered enough pharma gade raw materials to make my own BAC for the next 10 decades. Living in Europe, having premade pharma grade is a nightmare.

Where did you order? Thanks

 

[AlexDavis43]

Control what you can, what's done is done. If you're keeping it, the sooner you get it in the refrigerator, the less degradation it'll experience. It would take extensive testing of that specific batch to know how fast it degenerates, but we know whatever it is, it's *less* in cold temps.

Also, reconstitute to 3ml. When HGH is damaged, and it starts to aggregate, experiments show the greater the concentration, the worse the aggregation is because, simply put, the molecules are crowded and bump into each other more, increasing the chance of sticking to each other.

Here's an illustration of how damaged (oxidized in this case) HGH forms more and larger aggregates with increased concentration:

View attachment 322703

citrate buffer pH 3

Jano,

I came across a study of HGH degradation in which, after some severe stress (low PH and 37c temps) of two samples, purity tested at 98% and 96%. I suspect they were surprised by this, and realized when comparing the larger peak area of the initial unstressed sample, that the insoluble and poorly soluble aggregates HGH is known to produce weren't eluting from the the column.

After compensating for this, purity was actually 68% and 81%,

Is this something you think should be applied to future stress testing? I could be mistaken, but I thought I saw results in which peptides had the same (or similar) purity before and after stressing, but the mass dropped, which sounds similar to what the researchers encountered here.

As far as I know the fact insoluble aggregates don't elute doesn't seem to be a factor considered by any of the labs in the UGL space.

Thanks in advance.

View attachment 322617

(bottom left page 49)

https://www.researchgate.net/profile/Tudor-Arvinte/publication/256430850_Mulinacci_HGH_oxidation_EJPB_2013/data/0046352285c2aee7b9000000/Mulinacci-HGH-oxidation-EJPB-2013.pdf?origin=publication_list

storage at 37 degrees C (99 degrees F)

TLDR: The presence of NaCL (salt), destabilizes HGH.

View attachment 322706

pH and temperature. What?

This is what is meant by cherry picking and misinterpreting studies.

Also: here





Also here





More:

 

[Ghoul]

citrate buffer pH 3



storage at 37 degrees C (99 degrees F)



pH and temperature. What?

This is what is meant by cherry picking and misinterpreting studies.

Also: here

View attachment 322736

View attachment 322737

Also here

View attachment 322738

View attachment 322739

More:

View attachment 322740

The primary result of destabilized rHGH is the creation of insoluble aggregates. Whether that's from agitation, oxidation, heat, UV, or acidic conditions.

Above .22um they're filtered prior to analysis.

Below .22um they get stuck in the chromatography column.

Testing purity without adjusting results for the loss of mass to those aggregates results in an inaccurate calculation of rHGH purity, and therefore fails to accurately measure degradation. Whether that's 0.5% or 30%.

Is there something you don't understand about that? Jano does.

 

[janoshik]

I believe the sterile water the Human Growth Hormone preparations are (or used to be, as most pharma is switching to pens) to be dissolved in is the best option for EU users, as it's readily available. I also do believe it ought to last longer than a single day, as those preparation were seldom single use if my memory serves me right.

I might have a couple spare minutes to check our turbidity measurements today with:

1) pure water
2) regular high purity dissolved GH
3) dissolved GH that failed our sterility testing and endotoxin hard
4) low purity GH

 

[Nidus]

I believe the sterile water the Human Growth Hormone preparations are (or used to be, as most pharma is switching to pens) to be dissolved in is the best option for EU users, as it's readily available. I also do believe it ought to last longer than a single day, as those preparation were seldom single use if my memory serves me right.

I might have a couple spare minutes to check our turbidity measurements today with:

1) pure water
2) regular high purity dissolved GH
3) dissolved GH that failed our sterility testing and endotoxin hard
4) low purity GH

This would be awesome and provide clarity! Thank you Jano!

 

[Bobik]

I believe the sterile water the Human Growth Hormone preparations are (or used to be, as most pharma is switching to pens) to be dissolved in is the best option for EU users, as it's readily available. I also do believe it ought to last longer than a single day, as those preparation were seldom single use if my memory serves me right.

I might have a couple spare minutes to check our turbidity measurements today with:

1) pure water
2) regular high purity dissolved GH
3) dissolved GH that failed our sterility testing and endotoxin hard
4) low purity GH

Great. Thanks bro

 

[hellogre2]

I believe the sterile water the Human Growth Hormone preparations are (or used to be, as most pharma is switching to pens) to be dissolved in is the best option for EU users, as it's readily available. I also do believe it ought to last longer than a single day, as those preparation were seldom single use if my memory serves me right.

I might have a couple spare minutes to check our turbidity measurements today with:

1) pure water
2) regular high purity dissolved GH
3) dissolved GH that failed our sterility testing and endotoxin hard
4) low purity GH

That might help lot of europeans GH users, where your only options are garbage UGL bac water, going through US-EU custom (and taking the risk of the package being seized) while paying 50-80$ shipping fees or invest in raw materials and do your own...