The Lobster International,EU and UK domestic HGH,PEPTIDES and Turkish Pharmacy

[Standfast]

the hgh i ordered has no vacuum in any of the vials i used so far... why is that?

the vials are supposed to have vacuums aren't they

Same on the ones I received, I also did GH serum test and only scored 23.9. With other brands I score from high 30's to 50.

 

[TheLobster]

Same on the ones I received, I also did GH serum test and only scored 23.9. With other brands I score from high 30's to 50.

Hello

You cannot be sure about HGH quality with a serum test alone. If you have any doubts, send it to Janoshik, my friend.

Let me know tracking number if you want to do it .

Thank you

 

[Standfast]

Hello

You cannot be sure about HGH quality with a serum test alone. If you have any doubts, send it to Janoshik, my friend.

Let me know tracking number if you want to do it .

Thank you

I agree on testing, just stating my experience based off personal historical data.

 

[TheLobster]

I agree on testing, just stating my experience based off personal historical data.

In my personal opinion, blood tests are meaningless for HGH. Everyone can show different results, and those results prove nothing at all for me

Thank you for respecting my ideas. However, as I said before, if you have any doubts, please send it to Janoshik, as they can provide the only accurate quality verification with real testing. I will cover half the cost.

Thank you

 

[brutalbuilt]

Same on the ones I received, I also did GH serum test and only scored 23.9. With other brands I score from high 30's to 50.

damn really... ima do a gh serum test too and see i hope its at least 30 or higher cause i ordered a lot of gh from these guys

i did a gh serum test with another brand and scored 30

 

[Standfast]

In my personal opinion, blood tests are meaningless for HGH. Everyone can show different results, and those results prove nothing at all for me

Thank you for respecting my ideas. However, as I said before, if you have any doubts, please send it to Janoshik, as they can provide the only accurate quality verification with real testing. I will cover half the cost.

Thank you

Thanks for the offer but as I don't intend on running them going forward it not something I'm interested in.

Best of luck!

 

[brutalbuilt]

Hello

You cannot be sure about HGH quality with a serum test alone. If you have any doubts, send it to Janoshik, my friend.

Let me know tracking number if you want to do it .

Thank you

so whats the reasoning why there is no vacuum in the hgh vials?

 

[RekTest]

so whats the reasoning why there is no vacuum in the hgh vials?

I cant answer as to why, but I do know a (different) source I use has had vacuum and then some vials dont have vacuum.
Ive never seen anyone ask him in his thread, never actually seen it mentioned so it didnt bother me

 

[biggerben69]

so whats the reasoning why there is no vacuum in the hgh vials?

USA Pharmacy Grade GH has no vacuum. It has nothing to do with the quality of gh in the vials.

 

[iris]

USA Pharmacy Grade GH has no vacuum. It has nothing to do with the quality of gh in the vials.

Could it be linked to increasing shelf life of the product?

 

[TheLobster]

Hello

For the international batch, there is no vacuum, while the EU domestic batch has vacuum

The reason for this difference is that my machine’s top press mechanism encountered a problem, and I haven’t been able to fix it yet as I can’t find a reliable technician for the job

However, I want to assure you that whether the vial has a vacuum or not, both batches are the same in quality and there’s nothing to worry about


Thank you for your understanding

 

[MyNameIsJeff]

Hello

For the international batch, there is no vacuum, while the EU domestic batch has vacuum

The reason for this difference is that my machine’s top press mechanism encountered a problem, and I haven’t been able to fix it yet as I can’t find a reliable technician for the job

However, I want to assure you that whether the vial has a vacuum or not, both batches are the same in quality and there’s nothing to worry about


Thank you for your understanding

Does that mean you buy the GH raws from China and then lyophilize it in your own facilities in Turkey?

Not having a vaccum in the vials could in principle lead to faster degradation through oxidation (unless the vial contains an inert gas like nitrogen). See below. So this should be of concern to customers.

Oxidation is a common degradation pathway that affects therapeutic proteins and peptides during production, purification, formulation, transportation, storage and handling of solid and liquid preparations. In the present work we review the scientific literature about structural and biological consequences of protein/peptide oxidation. Representative examples are discussed of specific products whose oxidation has been recently studied, including monoclonal antibodies, calcitonin, granulocyte colony-stimulating factor, growth hormone, insulin, interferon alpha and beta, oxytocin and parathyroid hormone. These examples illustrate that oxidation often leads to modifications of higher-order structures, including aggregate induction, and can generate products that are pharmacokinetically different, biologically less active and/or potentially more immunogenic than their native counterpart. It is therefore crucially important during the pharmaceutical development of therapeutic proteins and peptides to comprehensively characterize oxidation products and evaluate the impact of oxidation-induced structural modifications on the biological properties of the drug.

Oxidation of therapeutic proteins and peptides: structural and biological consequences - PubMed

Oxidation is a common degradation pathway that affects therapeutic proteins and peptides during production, purification, formulation, transportation, storage and handling of solid and liquid preparations. In the present work we review the scientific literature about structural and biological...

pubmed.ncbi.nlm.nih.gov

Oxidation, particularly of methionine residues, is one of the major chemicaldegradations of proteins. In a previous publication we studied the conformation of recombinanthuman growth hormone (r-hGH) selectively oxidized at Met14 and Met125. Conformation ofoxidized r-hGH was found not different from that of nonoxidized r-hGH. In this paper, theeffect of methionine oxidation on the thermal stability of r-hGH folding was investigated. Thethermally induced unfolding process of the oxidized and nonoxidized protein was measuredby monitoring the circular dichroism signal at 220 nm. The melting temperatures (Tm) of theoxidized and nonoxidized r-hGH forms were determined at different pHs and in the presence ofsalts often used in pharmaceutical formulations. The effect of the location of the oxidized Metresidue in the protein and the percentage of oxidation were investigated. Our findings indicatethat the monoxidation of the two most accessible methionine residues of r-hGH—Met14 andMet125—has no effect on the protein conformation. However, oxidation of these residues to formsulfoxides does influence the thermal stability of the protein folding. The presence of the polar oxygen atom on the methionine sulfoxide group thermally destabilizes r-hGH folding. The effect(Tm) depends upon pH, ionic strength, and the location of the oxidized methionine residues inthe protein. The thermal melting of r-hGH and its oxidized products is a highly cooperativeprocess. Methionine oxidation leads to a thermal destabilization of the whole protein folding and is not just a local destabilization.

https://www.jpharmsci.org/article/S0022-3549%2815%2932294-2/pdf

 

[brutalbuilt]

USA Pharmacy Grade GH has no vacuum. It has nothing to do with the quality of gh in the vials.

have you even tried pharma? lol all the serostim kits ive had all had a vacuum

 

[MyNameIsJeff]

have you even tried pharma? lol all the serostim kits ive had all had a vacuum

Even if they do not have a vacuum, pharma products will at least be filled with an inert gas instead of air.

 

[MyNameIsJeff]

Prolonged exposure of lyophilized peptides and solutions (especially at high pH) to atmospheric oxygen should be minimized.

https://www.sigmaaldrich.com/NL/en/technical-documents/technical-article/research-and-disease-areas/cell-and-developmental-biology-research/peptide-stability

 

[MyNameIsJeff]

Another important consideration: A vacuum also helps to protect the lyophilized cake from exposure to atmospheric moisture, which could further destabilize the peptide. In a 2 mL vial with atmospheric air at 50% relative humidity, there would be approximately 23 micrograms of water in the air. How much degradation will that cause? Who knows...

 

[iris]

So vacuuming protects the peptide, prevents oxidation and exposure to humidity.

 

[AlexDavis43]

Even if they do not have a vacuum, pharma products will at least be filled with an inert gas instead of air.

Some anecdotal data points; not sure what to make of them:

For fat soluble compounds like gear, we always used nitrogen gas in the lab. Zero vacuum.

Vacuum is much more common among peptides.

Some vials of UGL gear have vacuum. My pharma TRT Test C has never had a vacuum.

 

[MyNameIsJeff]

Some anecdotal data points; not sure what to make of them:

For fat soluble compounds like gear, we always used nitrogen gas in the lab. Zero vacuum.

Vacuum is much more common among peptides.

Some vials of UGL gear have vacuum. My pharma TRT Test C has never had a vacuum.

I should have been more specific. My comments on vacuums apply only to pharma peptides, not AAS.

 

[brutalbuilt]

Another important consideration: A vacuum also helps to protect the lyophilized cake from exposure to atmospheric moisture, which could further destabilize the peptide. In a 2 mL vial with atmospheric air at 50% relative humidity, there would be approximately 23 micrograms of water in the air. How much degradation will that cause? Who knows...

well wish i knew there was no vacuum with this gh before i placed my big ass order