mercury
New Member
master you are confusing and overloading the customer with information he already told that he has access to GCMS only.
So let's stay with GCMS.
C18 column is only for HPLC, GCMS requires different type of columns.
MESO-Rx
Anabolic Steroids
Which Lab Test Should I Use?
- Thread starter Turkish Pharmacy
- Start date
master you are confusing and overloading the customer with information he already told that he has access to GCMS only.
So let's stay with GCMS.
C18 column is only for HPLC, GCMS requires different type of columns.
They have both HPLC and GC MS here is what they can do:
--------------------------------
GCMS:
Qualitative GC-MS Analysis
Quantitative GC-MS Analysis
Sample Preparation
Qualitative GC-MS Head Space
Quantitative GC-MS Head Space
GC-MS Thermal Desorber
HPLC:
Analysis conducted by DAD detector
Analysis conducted with a
fluorescence detector
Fluorescence detector + Derivatization
---------------------
I am also very overloaded. Still couldnt proceed with a result
master.on
New Member
I'm sorry for overloading youThey have both HPLC and GC MS here is what they can do:
--------------------------------
GCMS:
Qualitative GC-MS Analysis
Quantitative GC-MS Analysis
Sample Preparation
Qualitative GC-MS Head Space
Quantitative GC-MS Head Space
GC-MS Thermal Desorber
HPLC:
Analysis conducted by DAD detector
Analysis conducted with a
fluorescence detector
Fluorescence detector + Derivatization
---------------------
I am also very overloaded. Still couldnt proceed with a result
I'll make it simple this time
1 Bring some stuff you want to test.
Or you can first bring veterinary stuff since it looks much less suspicious:
much more vet gear is legally sold every year than human grade.
Tell her "you have been hired by ranchers to conduct independent quality assurance tests".
2 Tell her that you need to test it with GC-MS equipment at least 5 times to make sure results don't vary much.
5 times ain't something I made up. This is a normal procedure for chromatogaphers.
To keep it simple: that's it for now
Keep us posted with her answer.
Even if she says: I don't have any experience on that / I don't know how to test for them, still no problem
we can devise a working method for it.
But that's it for now.
mercury
New Member
HPLC:
Analysis conducted by DAD detector
Analysis conducted with a
fluorescence detector
You kept saying only GCMS,. Now you say that there is HPLC
I am guessing it is Agilent 1100. You do not need fluorescence detector for steroids testing only DAD.
You need to buy column for them it is about $800 and standards.
I have never been to Turkey if you buy me a ticket, 5 days in hotel and hot chick as a guide I could fly and set it up for you.
All are good to go but we rarely have hot chicks here. The testosterone you are using seems to work tho
Here is what they can do with HPLC:
Code:
https://thinksteroids.com/community/threads/which-lab-test-should-i-use.134392905/#post-2192353Anyway I am sending first samples next week hope they can figure it out. Please pray for me guys I know there are people from every religion here eventually one of the prayers will do the job, I will pray as the paganist-satanist
One question, do you think the database includes stuff like masteron enanthate? Or NPP? I want to get unpopular products tested to see if a lab is really serious and puts raw content as they should. I am pretty sure about all UGLs put testosterone enanthate into testosterone enanthate bottle.
master.on
New Member
@Turkish Pharmacy
a database is only useful to identify the steroids.
To quantify (how much is there) you'll need reference standards
Please ask her where do they buy the reference standards from
So you can surf their websites and see if they have the proper ones for the steroids that you intend to test.
Getting reference standards is crucial, even SIMEC turned down/delayed some testing as they didn't have the standards at hand.
OTOH Acceptable standards can be made by diluting true lab grade steroids.
a database is only useful to identify the steroids.
To quantify (how much is there) you'll need reference standards
Please ask her where do they buy the reference standards from
So you can surf their websites and see if they have the proper ones for the steroids that you intend to test.
Getting reference standards is crucial, even SIMEC turned down/delayed some testing as they didn't have the standards at hand.
OTOH Acceptable standards can be made by diluting true lab grade steroids.
Can GCMS identify the esters of steroids that are rarely used, like masteron enanthate?
I talked with them, I will get them other sample in injectable form that I am sure about the content. I can never be %100 sure so I dont know what will happen if they dont match each other. They told me I need the second one and that is going to be reference to see the mg of the product. They can determine the content(GC MS I think) but they cant determine the mg without reference so I will need to find reference.
I will send a ugl prop to be tested, for reference I will send 50 mg farmaks that come directly from Ukraine which I am pretty sure about the content. Its 50 mg/ml I dont know if I should say it beforehand.
mercury
New Member
I already wrote you that it can, it has huge data base including masteron.
They will handle it, the trick is only sample preparation and setup. I do not see why they wouldn't know how to do this.
master.on
New Member
If they're going to use GC-MS for quantifying (not as accurate as HPLC)
the first step is to repeat the test 5 times with the same sample to make sure the peak heights in the chart don't vary more than 1%
if it does, then GC-MS can't be used for quantification.
For best results they should do 5 repeat tests at 100%, 75%, 50% concentration
(and only once for 0%)
as to make sure peak heights don't vary along the calibration curve.
No reference standards are needed for this early, but crucial tests.
mercury
New Member
please stop giving confusing advices, this is not correct. The type of instrument chosen depends on type of analysis.
GC/MS is very sensitive instrument but it has to be handled in proper way.
and they do not need to test 5 times, once the method of testing is established.
master.on
New Member
Then why ALL pharmaceutical quality control departments all around the world use HPLC and not GC-MS?
OTOH pharma QC needs really accurate instruments (way less than 1% variation, often less than +-0.25% )
if all tested samples sit outside of +-15% or a single unit out of +-25%limits, then they need to recall the whole batch.
UG gear testing doesn't need this accurate, +-5% would be accurate enough.
At this stage they're setting up the method
so 5 times testing is needed to ensure repeatability, crucial for accurate testing.
Pharma QC do repeat tests every month, to ensure the machine remains accurate.
No big deal. These days even the smallest labs have autosamplers (devices that automatically insert the samples), so they can even leave the machine working overnight unattended.
Last edited:
Sent the first sample, it will come back in one week hopefully. I am just not %100 sure about reference sample's content. I would be glad if someone can show me how to do it. If steroid powder sellers sell it, which one should we go with. I take this lab test thing very seriously.
BTW they have hplc but they said they will use GC MS for both content and mg.
Also I found a friend who works in same university, a very old friend I know him since forever. He knows a bit about lab stuff he is handling(not testing personally of course but arranging it) it he is also trying to learn to help me out and maybe in future to do lab tests inside Turkey, no one is aware of lab tests here even people who use the machines
A wholeseller was trying to sell me stuff that I know is shit, he told me I can get them lab tested if I want. I told him I will get the stuff tested he was shocked saying how? How can you do it? How? ... How do you know how to test? Are you sure you can test it? Anyway, he is not trying to sell it anymore.
BTW they have hplc but they said they will use GC MS for both content and mg.
Also I found a friend who works in same university, a very old friend I know him since forever. He knows a bit about lab stuff he is handling(not testing personally of course but arranging it) it he is also trying to learn to help me out and maybe in future to do lab tests inside Turkey, no one is aware of lab tests here even people who use the machines
A wholeseller was trying to sell me stuff that I know is shit, he told me I can get them lab tested if I want. I told him I will get the stuff tested he was shocked saying how? How can you do it? How? ... How do you know how to test? Are you sure you can test it? Anyway, he is not trying to sell it anymore.
master.on
New Member
If 5 times repeat costs too muchSent the first sample, it will come back in one week hopefully. I am just not %100 sure about reference sample's content. I would be glad if someone can show me how to do it. If steroid powder sellers sell it, which one should we go with. I take this lab test thing very seriously.
BTW they have hplc but they said they will use GC MS for both content and mg.
Also I found a friend who works in same university, a very old friend I know him since forever. He knows a bit about lab stuff he is handling(not testing personally of course but arranging it) it he is also trying to learn to help me out and maybe in future to do lab tests inside Turkey, no one is aware of lab tests here even people who use the machines
A wholeseller was trying to sell me stuff that I know is shit, he told me I can get them lab tested if I want. I told him I will get the stuff tested he was shocked saying how? How can you do it? How? ... How do you know how to test? Are you sure you can test it? Anyway, he is not trying to sell it anymore.
see if they can test the same sample 3 times
this is crucial to make sure peak heights don't vary much (should be less than 1% variation).
If it does, then they must switch to HPLC for quantification and leave GC-MS for identification.
They told me(us) they will make it 5 times already, not requesting any extra payment for it. And they told me they will get it both done with GCMS. Maybe I should tell my friend who handles the job write here instead of me, he can respond more accurately.
So for reference samples, for now we are planning to make it ourselves. Which steroid powder seller is the best way to go? Is there any other method of sample making? I couldnt figure out hopefully my lazy friend will write here and ask for it in more detail he is smarter than me C'mon Mr.Greek
Does any provider sell "prepared" reference sample that we can trust? As far as I understand if our reference isnt good, the test wont mean anything.
C'mon Mr.Greek Does any provider sell "prepared" reference sample that we can trust? As far as I understand if our reference isnt good, the test wont mean anything.
master.on
New Member
IMO don't
even if gear is legal there, don't
academic research and gear don't mix
these days you can text/email/whatsapp him and ask tech questions.
Ready made reference standards:So for reference samples, for now we are planning to make it ourselves. Which steroid powder seller is the best way to go? Is there any other method of sample making? I couldnt figure out hopefully my lazy friend will write here and ask for it in more detail he is smarter than me
Does any provider sell "prepared" reference sample that we can trust? As far as I understand if our reference isnt good, the test wont mean anything.
https://www.cerilliant.com/ShopOnline/Product_Cat_List.aspx?text=testosterone
Testosterone
Search | Cayman Chemical
Testosterone (CRM)
https://www.lipomed.com/media/archive1/download/2013-10-10_Flyer_Steroids.pdf
You can also buy real lab-grade steroids (not underground steroids) and make acceptable reference standards as long as they are accurately weighed and diluted.
I heard of a guy who used big pharma gear (finished formulations) as improvised "reference standards" (albeit for HPLC but it should work for GC-MS)
This might be the simplest way to start:
Run a test of UG gear, and compare the peak heights to legit, big pharma gear.
If needed, dilute one of them so their concentrations match each other.
You can easily buy big pharma gear in Turkey so that shouldn't be a problem.
master.on
New Member
IMO don't
even if gear is legal there, don't
academic research and gear don't mix
these days you can text/email/whatsapp him and ask tech questions.
Ready made reference standards:So for reference samples, for now we are planning to make it ourselves. Which steroid powder seller is the best way to go? Is there any other method of sample making? I couldnt figure out hopefully my lazy friend will write here and ask for it in more detail he is smarter than me
Does any provider sell "prepared" reference sample that we can trust? As far as I understand if our reference isnt good, the test wont mean anything.
https://www.cerilliant.com/ShopOnline/Product_Cat_List.aspx?text=testosterone
Testosterone
Search | Cayman Chemical
Testosterone (CRM)
https://www.lipomed.com/media/archive1/download/2013-10-10_Flyer_Steroids.pdf
You can also buy real lab-grade steroids (not underground steroids) and make acceptable reference standards as long as they are accurately weighed and diluted.
I heard of a guy who used big pharma gear (finished formulations) as improvised "reference standards" (albeit for HPLC but it should work for GC-MS)
This might be the simplest way to start:
Run a test of UG gear, and compare the peak heights in the chart to legit, big pharma gear.
If needed, dilute one of them so their concentrations match each other.
You can easily buy big pharma gear in Turkey so that shouldn't be a problem.
Again, a great, simple and cheap way to get started in testing.
master.on
New Member
i.e. if you want to test some underground Test E 500
and you have big-pharma Test E 250 (i.e. Primoteston) as an improvised "reference standard" you need to dilute the Test E 500 by half, so their concentrations match.
You can even dilute the big-phama "reference standard" to create a calibration curve.
100% (no dilution), 75%, 50%, 25%, 0% (just bare oil+ BA)
then see where the peak heights lie in between,
and you have an approximate purity figure.
Please keep us posted on the results.
and you have big-pharma Test E 250 (i.e. Primoteston) as an improvised "reference standard" you need to dilute the Test E 500 by half, so their concentrations match.
You can even dilute the big-phama "reference standard" to create a calibration curve.
100% (no dilution), 75%, 50%, 25%, 0% (just bare oil+ BA)
then see where the peak heights lie in between,
and you have an approximate purity figure.
Please keep us posted on the results.
Hey guys, here he comes:
Code:
https://thinksteroids.com/community/threads/aegean-analyzer-steroid-lab-tests.134394777/Similar threads
