Enhanced Testing Database

[malomkarom]

GD dude. Appreciate your honesty and integrity. Good man. Thanks for sharing and your testing.

Honestly, yeah.This is the type of transparency I'd be willing to lose a cheeck for once I'm set on a source. 1 cycle with pippy test isn't the end of the world for a guarantee that what you bought is in there and gets to your door.

 

[readalot]

Hey read, I was curious if you have setup a master spreadsheet with all the info for easier tracking/navigating and wouldn't mind posting it. If not you should consider compiling one as it would be a huge QoL for everyone that is interested in this.

Thanks for making your new member intro.

This is a great idea but I haven't made one. Almost all of the activity has been logged here AFAIK. You'll just have to work your way through the detour in the thread. At some point for this to catch on others would need to get engaged on tool development. Would be a nice spreadsheet just to see vendor engagement with the ET but would be tremendous work keeping it up to date. Maybe even a subforum under the analytical testing forum.

Thanks for sharing your thoughts.

 

[readalot]

@DevonPharmacy raws testing via HPLC

MESO-Rx Sponsor Post in thread 'DEVON ONE STOP SHOP - US DOMESTIC'

Feb 11, 2025

New test result!
More to come very soon!
Also thanks a lot @readalot for encouraging me to try these tests.

Methasterone Raw Test Result!
Click here to view

• DevonPharmacy

• 
• 

Feel free to post your raws testing and ET here Devon if you'd like. Moving forward, I won't be logging all the activity as thoroughly as I was.

 

[DevonPharmacy]

New test result!
Stanozolol Raw Test Result;
Click here to view

Proviron Raw Test Result;
Click here to view

 

[DevonPharmacy]

Noxy Labs - Masterone Propionate Endotoxin Test;
Click here to view

 

[Exhortae]

Just got the result for Pharmacom endotoxin test.

 

[Sampei]

Just got the result for Pharmacom endotoxin test.

Holy shit first time we see something that is not 0.00x

@janoshik could this be because you are doing it in house now? Compared to the old tests

 

[Exhortae]

Btw my CRP. I am injecting this stuff

 

[janoshik]

Holy shit first time we see something that is not 0.00x

@janoshik could this be because you are doing it in house now? Compared to the old tests

Could be.

Still far below the limits as far as I checked.

 

[Sampei]

Could be.

Still far below the limits as far as I checked.

Thanks Jano, always on point

 

[janoshik]

Thanks Jano, always on point

My pleasure.

Personally oversaw the tests today.

Made some pictures and this is the third nicest thing I pictured after our CSO and her assistant. Calibration curve and stuff.

 

[Exhortae]

Could be.

Still far below the limits as far as I checked.

I thought the limit for injectable medication was 0.2 EU/ml

 

[readalot]

Just got the result for Pharmacom endotoxin test.

Thank you so much for ordering the test and having the sample checked.

@janoshik could this be because you are doing it in house now? Compared to the old tests

Very interesting. I wonder if previous testing was actually recovering the endotoxin from oil matrix properly or we had a bunch of false negative results up until now.

Still far below the limits as far as I checked.

Pharma Test cyp C of A shows limit NMT 0.88 EU/mg.

503b compounder sets even higher.

Great that you have a calibrated endotoxin test for oils now @janoshik. I know IP is a concern but were you able to show close to 100% recovery with your in house method?

I thought the limit for injectable medication was 0.2 EU/ml

Thanks to your help we are getting closer to the real story. We need to confirm absolute or relative specs with respect to pharma testing vs Jano's method.

Well done!

For reference:

Compounding pharmacy

R

Post in thread 'Readalots Enhanced Testing'

Nov 6, 2024

COA from the 503B

You can compare against the big pharma COA in the Source QC thread. The big pharma one much more exhaustive. To your point @B Ware the compounding pharmacies may share raws suppliers with some UGL vendors. It's a great question.

B Ware's HPLC test on same product (different batch).

• readalot

• 

Pharma

R

Post in thread 'Source QC and C of A (do you have one?)'

Jan 11, 2024

Could add in a few positive controls with pharma or compounded pharma products although the absolute concentration of impurities will be lower than in the raws obviously.

Ok, I am going to start with the simplest example I could think of that may be of interest to members. Getting a C of A from Pharma isn't as easy as it used to be. This took a little work with persistence. I have sanitized some of the details from this one to protect manufacturer confidential information as per forum Terms and Rules.

This is an FDA approved Testosterone Cypionate product manufactured in...

• readalot

• 

 

[Ateam2023]

Thank you so much for ordering the test and having the sample checked.


Very interesting. I wonder if previous testing was actually recovering the endotoxin from oil matrix properly or we had a bunch of false negative results up until now.




Pharma Test cyp C of A shows limit NMT 0.88 EU/mg.

503b compounder sets even higher.

Great that you have a calibrated endotoxin test for oils now @janoshik. I know IP is a concern but were you able to show close to 100% recovery with your in house method?



Thanks to your help we are getting closer to the real story. We need to confirm absolute or relative specs with respect to pharma testing vs Jano's method.

Well done!

For reference:

Compounding pharmacy

R

Post in thread 'Readalots Enhanced Testing'

Nov 6, 2024

COA from the 503B

You can compare against the big pharma COA in the Source QC thread. The big pharma one much more exhaustive. To your point @B Ware the compounding pharmacies may share raws suppliers with some UGL vendors. It's a great question.

B Ware's HPLC test on same product (different batch).

• readalot

• 

Pharma

R

Post in thread 'Source QC and C of A (do you have one?)'

Jan 11, 2024

Could add in a few positive controls with pharma or compounded pharma products although the absolute concentration of impurities will be lower than in the raws obviously.

Ok, I am going to start with the simplest example I could think of that may be of interest to members. Getting a C of A from Pharma isn't as easy as it used to be. This took a little work with persistence. I have sanitized some of the details from this one to protect manufacturer confidential information as per forum Terms and Rules.

This is an FDA approved Testosterone Cypionate product manufactured in...

• readalot

• 

Between you and @Ghoul , You two are "the cross reference KINGS" always on top of facts,,, Well done , ,

 

[Exhortae]

Thanks readalot.

Seems like this 0.625 EU/ml for pharmacom is actually within limits.

If there is a need to provide pharma grade testosterone oils I can (Have Test E amps from Bayer).

 

[janoshik]

Thank you so much for ordering the test and having the sample checked.


Very interesting. I wonder if previous testing was actually recovering the endotoxin from oil matrix properly or we had a bunch of false negative results up until now.

Me too.

Spiked samples we're getting 80-120% now.

EU pharmacopeia states 5 EU/kg for people for IV, so for a 220 lbs man using 1 ml of 500 EU/ml AAS oil would be permissible, if I interpret it correctly. I don't see subq or IM application there. Please, check me, I'm ill and tired.

 

[Exhortae]

For IM this is what I get. Simple TRT. 100 mg per week, for a bodyweight of 100kg. Test E (250 mg /ml)

K-value for IM injections: 5 EU/kg

I get 1250 EU/mL as maximum allowed endotoxin limit

For my TRT I am 10 000 times below the safety limit

 

[readalot]

Pharma Test cyp C of A shows limit NMT 0.88 EU/mg.

Note the mg above should be per mg of ester.

So using that pharma spec with 200 mg/ml oil concentration gives

0.88 EU/mg × 200 mg/ml = 176 EU/ml (per ml of product).

See also
1.

USP Monographs: Testosterone Injectable Suspension

2.

https://azupcriversitestorage01.blob.core.windows.net/storage-account-container/resources/doc_a/SimplifiedEndotoxinTestMethodforCompoundedSterileProducts.pdf

3.

https://www.acciusa.com/pdfs/whitepapers/EndotoxinLimits_SeanJH-PR17-012.pdf

Look at example 3 of the last pdf for parenteral injection...

Assume

250 mg / ml concentration
100 kg person

5 EU/kg ÷ (250 mg / 100 kg man) = 2.5 EU / mg Test ester

2.5 EU /mg Test ester × 250 mg/ml = 625 EU /ml product

TLDR: concur with @Exhortae 's and @janoshik 's comments.

Compounders spec shown above is very stringent and way below USP standard. The pharmacom sample still safely below even that spec.

Great to hear you confirmed 80 to 120% recovery, Janoshik. Thank you for sharing that info.

 

[readalot]

Note the mg above should be per mg of ester.

So using that pharma spec with 200 mg/ml oil concentration gives

0.88 EU/mg × 200 mg/ml = 176 EU/ml (per ml of product).

See also
1.

USP Monographs: Testosterone Injectable Suspension

2.

https://azupcriversitestorage01.blob.core.windows.net/storage-account-container/resources/doc_a/SimplifiedEndotoxinTestMethodforCompoundedSterileProducts.pdf

3.

https://www.acciusa.com/pdfs/whitepapers/EndotoxinLimits_SeanJH-PR17-012.pdf

Look at example 3 of the last pdf for parenteral injection...

Assume

250 mg / ml concentration
100 kg person

5 EU/kg ÷ (250 mg / 100 kg man) = 2.5 EU / mg Test ester

2.5 EU /mg Test ester × 250 mg/ml = 625 EU /ml product

TLDR: concur with @Exhortae 's and @janoshik 's comments.

Compounders spec shown above is very stringent and way below USP standard. The pharmacom sample still safely below even that spec.

Great to hear you confirmed 80 to 120% recovery, Janoshik. Thank you for sharing that info.

Also note paragraph from first page of reference 3...

What are Endotoxin Limits?
An endotoxin limit of 5 EU/kg/hr or 350 EU per adult (70 kg) was scientifically established to avoid the fever and hypotension from IM or IV injection of endotoxin contamination.2

Time is a factor because there are mechanisms in the liver and blood that neutralize endotoxin. However, there are no clearance mechanisms in intraspinal
spaces, so the IT (intrathecal) endotoxin limit is much more
stringent. An IT limit of 0.2 EU/kg/hr was arbitrarily set
without supporting experimental data. A more scientifically sound IT endotoxin limit of 14 EU per day avoids the serious mortality and morbidity associated with the signs of
meningitis that are induced by endotoxin in IT spaces.1
An Endotoxin Unit (EU) is a unit of biological activity of the USP Reference Endotoxin Standard.

 

[readalot]

Between you and @Ghoul , You two are "the cross reference KINGS" always on top of facts,,, Well done , ,

You are too kind. And here I was thinking I'm just a whiny c*nt, lol.

Now @Ghoul, that's a different matter. A knowledge KING.