MESO-Rx

Anabolic Steroids

Godt240 GODTROPIN 24IU - MALDI-MS; HPLC; UV - 2017/06 - Analyzer

Analyzer said:
I am posting these reports on request of @godt240 .
I have received these samples from him about a month ago, marked just with numbers. Mass spectrometry confirm presence of 191 AA hGH with molecular weight 22.129 kDa and its dimer. No other contaminants were detected. Dimer was found in about 15% of total protein amount, however initially it could be lower. I had some issues with mass spec so the samples were waiting reconstituted in fridge for about two weeks. This could lead to increased levels of dimer. Quantitative assays show total protein content 8.2 and 9.3 mg respectively. According to customer, these samples come from batches 160517 and 120617.
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What testing was done to confirm the sample was in fact a "protein".
 
godt240 said:
remember one thing, u will never satisfy people on meso. so just do what u want, leave drama here.
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Matt some of us did warn you that sending a sample direct from you to testing facility such as Analyzer would be questioned as potentially biased. Don't be surprised that's what happened! You say less 'drama' on Eroids, that's because it is primarily a heavily moderated source biased board. This is an members orientated board, there will always be questions and debate...
 
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Dr JIM said:
It's a VERY crude "estimate" at best but a certain degree of dimerization is EXPECTED with PEPS and P-PEPs
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Yeah he had said that it could be inaccurate if I understand right because of the time frame from when he reconstituted the GH till he actually was able to test it.
 
Naugahyde said:
Matt we did warn you that sending a sample direct from you to testing facility such as Analyzer would be questioned as potentially biased. Don't be surprised that's exactly what happened! For the record I'm not saying your Gh is bunk at all, just the testing method used wasn't ideal. If you are willing to finance anonymous testing again here that's great, if not I'm sure people will appreciate you've had enough of the 'meso way' for now! You say less 'drama' on Eroids, that's because it is primarily a source biased board. Here it is the members that rule. There will always be questions and debate, don't take it personally!
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I view it as he did exactly what members asked him to do.
 
Ripped said:
Yeah he had said that it could be inaccurate if I understand right because of the time frame from when he reconstituted the GH till he actually was able to test it.
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The USP standard for GH testing remains Amino Acid Analysis.

The difference is important bc a 100 dalton mean MW variance can be overlooked by LC/MS.
 
Dr JIM said:
The USP standard for GH testing remains Amino Acid Analysis.
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I'm not an Authority on testing that's for sure but there seems to be alot of controversy with AAA testing is the what's used by Big pharma?
 
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Yeah it wasn't ideal bro. but matt was doing what was asked of him.
It best for an anonymous member to send samples .
 
How much money are we talking about in total for me to anonymously send a sample? Shipping, lab cost, everything.
 
Ripped said:
I'm not an Authority on testing that's for sure but there seems to be alot of controversy with AAA testing is the what's used by Big pharma?
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Of course that's what Big Pharma uses in addition to several other assays including LC/MS.

Make no mistake about it NO UGL compiles with big pharma
standards bc testing at that level
would significantly increase the cost of GGH.

Oh and PED forums are the only place AAA testing is considered "controversial". In fact ALL of the testing @mands and I did complied with that standard.
 
Just saw a YouTube video posted by EA and one of the guys in the UK said that they have a place that can test their gear and soon GH. A big name brand ugl went out of business due to their vials being way underdosed. Check it out. Start at 7:00 in the video.
 
TRT said:
Just saw a YouTube video posted by EA and one of the guys in the UK said that they have a place that can test their gear and soon GH. A big name brand ugl went out of business due to their vials being way underdosed. Check it out. Start at 7:00 in the video.
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Hey thanks mate, do you have the title of the video or link by any chance?
Believe it or not we used to have a free harm reduction drug testing service here in the UK that tested anabolic steroids, but they were so overwhelmed with samples they stopped testing! They still exist but now only for testing narcotics/street drugs.
 
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Naugahyde said:
Hey thanks mate, do you have the title of the video or link by any chance?
Believe it or not we used to have a free harm reduction drug testing service here in the UK that tested anabolic steroids, but they were so overwhelmed with samples they stopped testing! They still exist but now only for testing narcotics/street drugs.
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It's on broke back fitness. My mistake.
"How I got a TRT script in the UK".
Another mistake. Not at 7mins. The video is only 6:45 long. It's a guy with a beard he ends up talking to at the expo or something.
 
TRT said:
It's on broke back fitness. My mistake.
"How I got a TRT script in the UK".
Another mistake. Not at 7mins. The video is only 6:45 long. It's a guy with a beard he ends up talking to at the expo or something.
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Thanks. Lots of misinformation from 'Tony Huge' in this video though. Apparently we 'can't get Trt in the UK and have to go Underground'.
Well I'm on NHS Doctor prescribed Trt and I'm in the UK! If you test below range you will be scripted Androgel, Sustanon or Nebido.
 
Dr JIM said:
The USP standard for GH testing remains Amino Acid Analysis.

The difference is important bc a 100 dalton mean MW variance can be overlooked by LC/MS.
Click to expand...


I am sorry to say this but there is no mention about Amino Acid Analysis in the Somatropin part of USP. Where did you get this information?

MW difference 100 Da can not be overlooked in any way by mass spectrometer. Usual accuracy is somewhere between 1 and 0.001 Da, depending on particular instrument.
 
A MS assay is the average MW of those compounds with a sample and IF the MW mean variance is close enough together a change as in deletion of one amino acid can readily be "missed".

For instance a variance of 0.01 to 0.001 for a sample with a MW of 22KD speaks to the issue directly bc in this instance such a variance equals 22 to 220 daltons.

Stated another way bc MS is an average MW of those compounds within a particular peak it lacks the specificity of an AAA.


The answer to your USP question is based on research conducted at the outset of RHGH development and testing for QC which became the eventual USP standard.
 
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