Godt240 GODTROPIN 24IU - MALDI-MS; HPLC; UV - 2017/06 - Analyzer

[TRT]

I am sorry to say this but there is no mention about Amino Acid Analysis in the Somatropin part of USP. Where did you get this information?

MW difference 100 Da can not be overlooked in any way by mass spectrometer. Usual accuracy is somewhere between 1 and 0.001 Da, depending on particular instrument.

A MS assay is the average MW of those compounds with a sample and IF the MW mean variance is close enough together a change as in deletion of one amino acid can readily be "missed".

For instance a variance of 0.01 to 0.001 for a sample with a MW of 22KD speaks to the issue directly bc in this instance such a variance equals 22 to 220 daltons.

Stated another way bc MS is an average MW of those compounds within a particular peak it lacks the specificity of an AAA.

The answer to your USP question is based on research conducted at the outset of RHGH development and testing for QC which became the eventual USP standard.

The two science nerds going at it. I love it, don't understand much of it but I love it! Lol

 

[TRT]

Thanks. Lots of misinformation from 'Tony Huge' in this video though. Apparently we 'can't get Trt in the UK and have to go Underground'.
Well I'm on NHS Doctor prescribed Trt and I'm in the UK! If you test below range you will be scripted Androgel, Sustanon or Nebido.

He wasn't wrong just making his own inference off of one person which we know isn't enough evidence to draw a conclusion from. I know watching that the guy from broke back mountain, I mean...broke back fitness, lol, wasn't doing what he should with his doctors. Which was demand it. They work for you not the other way around. I've said this 100x America, UK, doesn't matter.

 

[Dr JIM]

I am posting these reports on request of @godt240 .



Mass spectrometry confirm presence of 191 AA hGH with molecular weight 22.129 kDa

total protein amount

however initially it could be lower. I had some issues with mass spec so the samples were waiting reconstituted in fridge for about two weeks. This could lead to increased levels of dimer.

Quantitative assays show total protein content 8.2 and 9.3 mg respectively.

I'd still like to know how this form of testing "confirms" the sample was PROTEIN or even GH for that matter?

 

[Dr JIM]

What other molecule with molecular weight 22 129 Da would you expect in the sample?

How presumptuous of you but let me suggest you use your imagination about how 191 Amino Acids can be arranged or rearranged in such a manner they are void of ANY biological activity.

And those who were involved in
developing rHGH from the outset
would LOL about that comment.

So no testing was done to confirm the sample was a protein!

That's called researcher bias fella and
I suspect the fact GT told you it was GH influenced your decision to report that which can not be determined from the assays you performed.

My suggestion just stick to the FACTS.

 

[Dr JIM]

Finally what methods were used to QUANTIFY these two GT samples.

It would help if your reply is specific.

 

[Ripped]

 

[Ripped]

I can't find any reference to AAA test of HGH or an approved method @Dr JIM can you please link information on AAA testing of GH sir?

 

[Dr JIM]

View attachment 72024

 

[Dr JIM]

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I can't find any reference to AAA test of HGH or an approved method @Dr JIM can you please link information on AAA testing of GH sir?

IDK what your looking for bc your comparing apples to oranges.

BC after years of testing, BP is conducting tests on a product they know is GH and are only looking for confirmation their quality remains the same from batch to batch, and the
foundation of their QQ was AAA, bc AAA is the gold standard by which the reliability of LC/MS was determined.

UGL have no foundation and their QQ remains spotty at best, so if you are others want to rely on an assay that is limited to the the average MW of those compounds within a given peak, (absent protein confirmation) that's fine by me.

The three research citations I'm referring two were posted on the
second UGL GH assays.

 

[TRT]

I think all three of you should fly out and see one another and settle this for the whole underground world. Bet you guys would get along and straighten it out pretty quick if you all admitted what you were or weren't capable of doing.

 

[Ripped]

IDK what your looking for bc your comparing apples to oranges.

BC after years of testing, BP is conducting tests on a product they know is GH and are only looking for confirmation their quality remains the same from batch to batch, and the
foundation of their QQ was AAA, bc AAA is the gold standard by which the reliability of LC/MS was determined.

UGL have no foundation and their QQ remains spotty at best, so if you are others want to rely on an assay that is limited to the the average MW of those compounds within a given peak, (absent protein confirmation) that's fine by me.

The three research citations I'm referring two were posted on the
second UGL GH assays.

Ok I will go back and look at it I'm just trying to get a better understanding of why actual analytic testers of GH have a different view of how it's tested.

 

[Dr JIM]

Ok I will go back and look at it I'm just trying to get a better understanding of why actual analytic testers of GH have a different view of how it's tested.

Do you really believe UGL GH producers want to be held to Big Pharma standards, seriously.

The overwhelming majority decry any form of analytical testing, unless "their designated lab" conducts the testing.

Ever heard of Jano LMAO !

 

[stone988]

Does anybody actually workout? or just piss,moan and complain and accuse and whatever on this board lol With all the effort and fucking around and wanking off most of you could have gotten a second job and could easily afford pharma HGH and wouldn't have to worry about generics! Jeez

 

[Docd187123]

Does anybody actually workout? or just piss,moan and complain and accuse and whatever on this board lol With all the effort and fucking around and wanking off most of you could have gotten a second job and could easily afford pharma HGH and wouldn't have to worry about generics! Jeez

I sincerely hope the sweet irony is not lost on anybody.

 

[Dr JIM]

Ok I will go back and look at it I'm just trying to get a better understanding of why actual analytic testers of GH have a different view of how it's tested.

Oh and if you would like further evidence about the pro GGH bias that dominates PED forums, try to locate one SOURCE board that allows members to cite their own analytical data, esp of the results conflict with the paid advertiser party line.

 

[XmadXscientist]

Could you please clarify what do you mean by micro specimens?

In my lab the microbiologist have an instrument they just recently got that shortens their specimen identification by days and they've been calling maldi. I never thought about what it was until now and found out we have a mass spec machine sitting in our building.

 

[Brolloks]

". In fact ALL of the testing @mands and I did complied with that standard.

What testing did you do? I thought you sent off samples to a lab rather than conduct your own testing, Jimmy?

 

[Ripped]

Oh and if you would like further evidence about the pro GGH bias that dominates PED forums, try to locate one SOURCE board that allows members to cite their own analytical data, esp of the results conflict with the paid advertiser party line.

No I just want a better understanding of what the accepted method of testing all GH is whether generic or brand. I'm neutral and not trying to take any side because there had been alot if back and forth .

 

[Ripped]

Oh and if you would like further evidence about the pro GGH bias that dominates PED forums, try to locate one SOURCE board that allows members to cite their own analytical data, esp of the results conflict with the paid advertiser party line.

Reddit sst.

 

[Naugahyde]

He wasn't wrong just making his own inference off of one person which we know isn't enough evidence to draw a conclusion from. I know watching that the guy from broke back mountain, I mean...broke back fitness, lol, wasn't doing what he should with his doctors. Which was demand it. They work for you not the other way around. I've said this 100x America, UK, doesn't matter.

Yea initially I had to be pushy with my Doctor, but he's cool now. Don't ask, won't get. I think Trt treatment in the UK is a bit behind the US, many Docs are just not aware of the issues so that part of the video was fair