Your only pic is a washing machine!!!!!!!!!! No pics of sterilization AT ALL !!!!!'nnnnm
-Myth-
-Myth-
MESO-Rx
Anabolic Steroids
Group home brew crowd funding project
- Thread starter shamrockbear
- Start date
shamrock...I've seen u for a few yrs around the boards, correct? You're gonna get yourself in trouble with all this. Why are you posting all your biz out in the wide open? This entire thread is beyond ridiculous.
I like you, partner, but I'm advising you to cease and desist this shit. In other words, knock it off.
I like you, partner, but I'm advising you to cease and desist this shit. In other words, knock it off.
shamrockbear1
New Member
Thanks bb69 your a salty vet like me so I will just go private just to avoid myth crying like a girl who just won the physique final. Plus bb69 you always keep it real....
You're a POS!!!!
Nice job JB
However the based peak, and any other AUC peak absorptions must be CALCULATED as proof what you assume to be dimers indeed are.
That's bc the formation of a dimer occurs in proportion to the reduction of the parent compound in this case TT.
Moreover dimer formation which occurs at such high frequency that it' EXCEEDS the concentration of the parent compound is highly suspect of overt contamination or ......
I'll take a closer look on my computer at home SB.
Yep that's the limitations of an iPhone,
Regs
Jim
The MS pic is NOT the "standard" SB. Although many of the operational functions are listed one which is NOT is the MS voltage and the UV wavelength used for the chromatograph. These are definitely standard lab procedure.
Also one need not perform an ACN dry run, it's not necessary as the results are readily reproducible.
Furthermore although the Na adduct seems on target the "TT fragments" listed are not at all common fragmentation pattern at 0.1 eMv for Testosterone. (By convention the eMv may be ASSUMED if it's not listed.)
Thats bc the generation of the fragments you listed, mandate the severance of covalent bonds and expose the outer electron ring. The latter is VERY unstable and certainly would not occur in the concentrations you have assumed, if they occurred at all.
MS fragmentation patterns for a variety of organic substances are listed in the Chemists Analytical Compendium available thru you local UGL for 19.99 plus shipping and handling. No really these Frag patterns are reasonably well established based on real time and statistical models. Their accuracy is actually quite good CONSIDERING THE POSSIBILITIES. hats bc the generation of the fragments you listed, mandate the severance of covalent bonds and expose the outer electron ring. The latter is VERY unstable and certainly would not occur in the concentrations you have assumed, if they occurred at all.
Lastly I hope the fella who performed these tests was NOT the one providing you with answers on purity and concentration, because I that regard its absolutely unfounded fella.
Here are a few reasons why, ALL of which my alter your purity and or concentration results.
First the UV absorbance WL is not mentioned (TT absorbs UA of different frequencies at different rates which most certainly effect the width and/or height of ANY LC peak.
Second, no calibration curve is depicted. The curve must first be mechanically generated by injecting varied dilutions of the sample into an LC to collate predictable X and Y axises data. The recovered data is then used to calculate purity and concentration information on submitted samples.
Finally bc looks can be deceiving, one can NOT LOOK AT an chromatograph and assume a specific concentration, period! I've seen that error all to frequently. This DATA can ONLY be obtained thru the use of a calibration curve. Thru the use of refined computer software (designed specifically for this indication) exacting AUC measurements are obtained of all spike wave activity, excluding background noise, and plotted (compared) to EACH OTHER for very accurate data.
Really SB although this analysis is an improvement over many of the analyses Ive seen It's still far short compared to certified lab standards!
Here is an FYI comparison from a CERTIFIED LAB
Yea even I was surprised at the low concentration, but I still can't believe David remains steadfast saying "I trust my raws".
F..ing pathetic!
Jim
Also one need not perform an ACN dry run, it's not necessary as the results are readily reproducible.
Furthermore although the Na adduct seems on target the "TT fragments" listed are not at all common fragmentation pattern at 0.1 eMv for Testosterone. (By convention the eMv may be ASSUMED if it's not listed.)
Thats bc the generation of the fragments you listed, mandate the severance of covalent bonds and expose the outer electron ring. The latter is VERY unstable and certainly would not occur in the concentrations you have assumed, if they occurred at all.
MS fragmentation patterns for a variety of organic substances are listed in the Chemists Analytical Compendium available thru you local UGL for 19.99 plus shipping and handling. No really these Frag patterns are reasonably well established based on real time and statistical models. Their accuracy is actually quite good CONSIDERING THE POSSIBILITIES. hats bc the generation of the fragments you listed, mandate the severance of covalent bonds and expose the outer electron ring. The latter is VERY unstable and certainly would not occur in the concentrations you have assumed, if they occurred at all.
Lastly I hope the fella who performed these tests was NOT the one providing you with answers on purity and concentration, because I that regard its absolutely unfounded fella.
Here are a few reasons why, ALL of which my alter your purity and or concentration results.
First the UV absorbance WL is not mentioned (TT absorbs UA of different frequencies at different rates which most certainly effect the width and/or height of ANY LC peak.
Second, no calibration curve is depicted. The curve must first be mechanically generated by injecting varied dilutions of the sample into an LC to collate predictable X and Y axises data. The recovered data is then used to calculate purity and concentration information on submitted samples.
Finally bc looks can be deceiving, one can NOT LOOK AT an chromatograph and assume a specific concentration, period! I've seen that error all to frequently. This DATA can ONLY be obtained thru the use of a calibration curve. Thru the use of refined computer software (designed specifically for this indication) exacting AUC measurements are obtained of all spike wave activity, excluding background noise, and plotted (compared) to EACH OTHER for very accurate data.
Really SB although this analysis is an improvement over many of the analyses Ive seen It's still far short compared to certified lab standards!
Here is an FYI comparison from a CERTIFIED LAB
Yea even I was surprised at the low concentration, but I still can't believe David remains steadfast saying "I trust my raws".
F..ing pathetic!
Jim
Incidentally ONE of many reasons HPLC is done BEFORE Mass Spec, is the inevitable formation of aggregates, fragments, dimers or even some adducts which often arise as a result of the MS ITSELF.
Thus if the HPLC is run first, accounting for chemical conjugates or parenteral fragments as a consequence of MS becomes relatively simplistic. (If they were NOT detected during the HPLC run, then THEY ARE the result of the MS process itself)
PS and any reasonable analytical chemist would KNOW this factoid.
jim
Thus if the HPLC is run first, accounting for chemical conjugates or parenteral fragments as a consequence of MS becomes relatively simplistic. (If they were NOT detected during the HPLC run, then THEY ARE the result of the MS process itself)
PS and any reasonable analytical chemist would KNOW this factoid.
jim
Last edited:
If I understand right this is nothing more than several members getting together to brew. Why would he need photos?
If that's the case BP why would SB have posted his accounts on Meso?
No it seems apparent to me SB's vying for much more and what a better place to start a a new business venture than on Meso with it's YOUNG captive, and in many ways gullible audience?
I've certainly no objection and have tried to limit my comments to those tests submitted by SB.
Regs
Jim
No it seems apparent to me SB's vying for much more and what a better place to start a a new business venture than on Meso with it's YOUNG captive, and in many ways gullible audience?
I've certainly no objection and have tried to limit my comments to those tests submitted by SB.
Regs
Jim
Shamrockbear what are your intentions. Is it your plan to source or to pool resources on a home brew?
We discussed this weeks ago, and it does make sense to me to allow other trusted members in on a brew. You will have the resources to test the raws you purchase before you brew. If it's a go you are in business, if not the pain is spread out and you look for a new raw.
If you already have other members interested, I would walk away from this thread.
We discussed this weeks ago, and it does make sense to me to allow other trusted members in on a brew. You will have the resources to test the raws you purchase before you brew. If it's a go you are in business, if not the pain is spread out and you look for a new raw.
If you already have other members interested, I would walk away from this thread.
Last edited:
shamrockbear
New Member
My intentions have always been the same test the raws and make appropriate dosage of gear. I would like to supply just a few guys. Im not going to go all main stream. My goal has been to be able to cover my costs associated with gear i spend on (me personally have spend $15K that includes some GH), I'm not greedy or looking to build New Jack City. I have been pretty straight forward.
Dr. Jim I really appreciate your input I did send this sample to a accredited large university lab and the testers title was "assistant professor" so I have to leave it in his hands for testing but I will forward the information to him because I am having 4 new test conducted right now. Also, I know Dr. Jim this does not compare to large companies but you need to understand they also have tons of money and equipment to conduct these tests as i do not.
Dr. Jim I also appreciate that you keep it civil and constructive like other on here.
thanks
SB
PS
Dr. Jim I have forwarded you analysis to the lab and I will post his response.
Dr. Jim I really appreciate your input I did send this sample to a accredited large university lab and the testers title was "assistant professor" so I have to leave it in his hands for testing but I will forward the information to him because I am having 4 new test conducted right now. Also, I know Dr. Jim this does not compare to large companies but you need to understand they also have tons of money and equipment to conduct these tests as i do not.
Dr. Jim I also appreciate that you keep it civil and constructive like other on here.
thanks
SB
PS
Dr. Jim I have forwarded you analysis to the lab and I will post his response.
Last edited:
I back you 100%. I can see no good reason to crash your party other than you posted it on our board
Marcus
New Member
In general it sounds like a good idea, but I'm a little confused. 2 post above you say that you are trying just to cover personal costs which add up to 15k (including GH), wouldn't that be funding your habit which would in turn be making a profit? Not that I'm against that, but than its against the principal of what your starting, correct?
Maybe I'm not understanding fully
Maybe I'm not understanding fully
shamrockbear1
New Member
Well of course cover my personal habit if I make a profit great why not...
It depends on what kind of profit we are talking about. You have made pubic your plan.
There are various forms of collectives. As long as the folks that choose to become involved know what they are getting into, more power to you.
I would not advise you to continue posting. It looks kinda shady.
There are various forms of collectives. As long as the folks that choose to become involved know what they are getting into, more power to you.
I would not advise you to continue posting. It looks kinda shady.
shamrockbear1
New Member
Ok I'll keep it strictly about the home brew
shamrockbear1
New Member
The labs response :
I am going to take a little bit of time to answer you and your Doctor Friend.
1. Your email is impossible to understand as it is written in poor English.
2. Your resident doctor might be a good physician but he is not an expert in Mass Spectrometry
3. The work that I performed for you did not state that I was going to do quantitation by HPLC with UV visible detection, only by LC-MS (by the way, LC is HPLC). It was not stated in my quotation for service either.
4. The acetonitrile run is required in order to ensure the observed peaks in the sample are NOT originating from impurities in the system or in the solvent. It is a standard practice. I quite can’t figure out the nature of your complaint, I did not charge for the acetonitrile run, only for your sample.
5. You are not familiar with the fragmentation process (MS/MS). This is done to confirm the identity of the compound. Seeing a peak on the LC-MS chromatogram which corresponds to the expected peak is NOT enough to assign the peak to the expected compound.
6. I do not have a laboratory performing tests for me. All the experiments are done in house
7. Since no standard UV work was requested, none was performed, so not calibration curve is necessary
8. I never reported a concentration, only a rough idea on what the purity might be. Also I clearly stated in my previous email from 089-27-14 that I would need an authentic standard in order to perform quantitation on the sample: “The purity is based only on the mass spec measurement. This is because the compound is difficult to measure and I don’t have an external standard to correlate the area for the TS peaks. For that an externals standard would be required.”
9. There is an LC in front of the MS. This is why the experiment I performed is and LC-MS. You and your resident doctor friend do not know what you are talking about.
I am going to take a little bit of time to answer you and your Doctor Friend.
1. Your email is impossible to understand as it is written in poor English.
2. Your resident doctor might be a good physician but he is not an expert in Mass Spectrometry
3. The work that I performed for you did not state that I was going to do quantitation by HPLC with UV visible detection, only by LC-MS (by the way, LC is HPLC). It was not stated in my quotation for service either.
4. The acetonitrile run is required in order to ensure the observed peaks in the sample are NOT originating from impurities in the system or in the solvent. It is a standard practice. I quite can’t figure out the nature of your complaint, I did not charge for the acetonitrile run, only for your sample.
5. You are not familiar with the fragmentation process (MS/MS). This is done to confirm the identity of the compound. Seeing a peak on the LC-MS chromatogram which corresponds to the expected peak is NOT enough to assign the peak to the expected compound.
6. I do not have a laboratory performing tests for me. All the experiments are done in house
7. Since no standard UV work was requested, none was performed, so not calibration curve is necessary
8. I never reported a concentration, only a rough idea on what the purity might be. Also I clearly stated in my previous email from 089-27-14 that I would need an authentic standard in order to perform quantitation on the sample: “The purity is based only on the mass spec measurement. This is because the compound is difficult to measure and I don’t have an external standard to correlate the area for the TS peaks. For that an externals standard would be required.”
9. There is an LC in front of the MS. This is why the experiment I performed is and LC-MS. You and your resident doctor friend do not know what you are talking about.
shamrockbear1
New Member
Additional response :
Also you need to know that the laboratory Director is an Associate Professor of Biochemistry with a background in BioAnalytical work and almost a decade of experience in Mass Spectrometry. My background is in Chemistry, Biochemistry, but I also have experience in Analytical work regarding method development and analysis in support of production of biotherapeutic proteins.
Also you need to know that the laboratory Director is an Associate Professor of Biochemistry with a background in BioAnalytical work and almost a decade of experience in Mass Spectrometry. My background is in Chemistry, Biochemistry, but I also have experience in Analytical work regarding method development and analysis in support of production of biotherapeutic proteins.
Well there in lies the difference in what was SUGGESTED by SB and what was posted by the lab
There is NO CONCENTRATION DATA SB, this you clearly implied and was depicted, yet is false
And I'll state it again you CAN not obtain reliable concentration data using a MS ALONE and your chemist just confirmed that very statement in his reply SB.
How in the heck can someone post data and suggest it's legitimate yet latter imply it needs to be confirmed?
I also understand an ACN run must be performed but it's not a routine practice to reveal it to consumers. (Did I ever said or suggest doing so was a violation of existing standards)
Not ONE of my statements have been contradicted SB, NONE!
The fact is SB is you have misrepresented the MEANING of the analysis your biochemist performed, and he doesn't seem very happy about it.
shamrockbear1
New Member
Damit can't this be just easy lol....
But I am enjoying the science part to this all..
But I am enjoying the science part to this all..
