Group home brew crowd funding project

[shamrockbear1]

Well I see your angle but LE can order from every source .. So not sure your point ? Astro, gear pro plus a ton of others are domestic no issues..

 

[shamrockbear1]

OK people I'm getting preliminary reports now but will have the whole thing done soon...got this from the lab like I said only preliminary... " The LC by itself does not confirm its purity, but the LC-MS does. I ran the sample twice today. I can clearly see the peak for testosterone, confirming it tonight by LC-MS/MS. However, I do see a couple of peak clusters centered at around 433 and 465, which I am also fragmenting tonight. At this point I would estimate the purity of the testosterone at about 33% since its peak and the other two clusters are about equal in intensity.

 

[shamrockbear1]

I'm kinda pissed with purity this low but told you guys these raws all are shitty...so that means I have to add 3 times the amount to equal proper dosage

 

[beedix]

Yikes..I wouldn't 3x anything. Although the mass spec testing source that seems to be frequently used is in question. Given all the results, not sure they know what they are doing.

Did the Enth pass the appearance/melting point test?

 

[shamrockbear1]

My lab is an independent lab not associated with anyone on this board. Its test cyp. I was testing it passed melting point.. It means if I made the gear without testing for one cc I would 66.66 mg which would of messed up anyones cycle. Shut you down would of passed labs and results would have been nominal

 

[Mr.Bravo]

Hey I hear you Bear, I would probably have aheart attack and be ready to cut diamonds if I could get something properly dosed.

 

[shamrockbear]

Okay, so for all you nay sayers and cocksuckers that gave me a hard time look at this PDF this is how all mass specs should be done better than any I have seen done yet period. I'm disappointed with the results at 25% but what can you do, Ohhhhh who wants to signup now?....
Here are the results explained!
See the attached Powerpoint slides. First I ran the LC-MS in reverse phase mode with positive detection. Mobile phase was water to acetonitrile (ACN) with 0.1% FA. Slide 2 shows that the peaks which elute during the gradient (30 min) are mainly associated with three peak clusters with maxima at 289, 433 and 865. From your information the peak at 289 is consistent with protonated testosterone. The other two are unknown compounds. They are not derived from the LC system, ACN or the mobile phase (see slide 3). Thus I extracted the chromatogram for each of the peaks at the said maxima and shown at the bottom (Slide 2) is the spectrum at ~ 19 min, consistent with testosterone and its Na adduct (m/z = 311). Slide 1 shows the same results for another sample preparated at 1in100 dilution in ACN. Basically the same testosterone peak is shown at 19 min. The more hydrophobic peaks for 433 and 866 are shown as spectra in the middle panel (Slide 1). The m/z= 866 might be a dimer of the m/z= 433 peak. Slide 3 shows a blank (ACN) injection. Slides 4 and 5 show the LC-MS/MS of the 1/100 sample tracking the fragmentation of the 289, 433 and 866 peaks. The 289 peak produces fragments with are consistent with the testosterone rings A and D (97 and 109) at 19 min. At 26.5 min the chromatographic peak (Slide 5) indicates that the 433 precursor is related to testosterone since it produces similar fragments plus a bit of the 289.4 precursor, presumably due to the loss of a side chain with mass of 144. The bottom MS/MS spectrum, also at 26.5 min (Slide 5) confirms that 866 is a dimer of 433. The Q1 spectra of the sample (not shown) shows that the charges of all the three peaks are +1. Based on the Q1(+) spectrum of the entire chromatogram (slide 2) and the fact that the intensities of 289 : 433 : 866 are in the ratio 1:2:1, it would appear that the ratios of the three compounds is 25%:50%:25% respectively. Thu testosterone is ~ 25% of the sample.

 

[shamrockbear1]

And yes I can mass spec products for people but because of the depth involved it costs a little more, but the best I have seen yet. Not even Angus or mythotic have results posted like this .... Personally, I thought you are getting scammed for those mass specs people have been posting ..
Also, since I'm not a scientist I can not really explain much other than posted I got directly from the lab.

 

[shamrockbear1]

No comments its like I hear crickets in here ... I guess when you do it right from the start I guess all the cynical people and critics don't have much to say....

 

[shamrockbear1]

Anyone want to run a log for me? need one or two vets free gear... What more can you ask for...

 

[clamz]

I understand what ypur going for bro and its a cool idea. but pretend ypu are just brewing one vial@ 250mg/ml. at 25% purity, you would have to suspend 10 grams in 10ml of oilwhich would be the same amount of powder as test cyp 1000 which i don't think exists. i have tried test e 500 and the pip was horrendous, if you double that and replace it with 25% pure cyp/75% garbage I'm pretty sure it will be torturous and probably dangerous. you would essentially be injecting 7.5 grams of complete garbage in the vial, complimented by 2.5 grams of pure test cyp. man that is a painful idea, you should trash it and start over.

 

[shamrockbear1]

Well I understand what your saying so I just displaced the oil and I have run it myself no problems. honestly the powder is such a small component and the BA and BB prevent any issues for sure, so I'm just looking for someone to run the test straight. Its to hard for me to do it while runnings orals or other gear... Also we all know all UGLs gear is anywhere from 50-85 % pure what do you think that filler is ? That's how they get it (powder) and just make it.. I don't see too many people worried about every other ugl..
Its funny I run mass specs have good gear but I'm still getting shit... [emoji50]

 

[Voltrader]

You have other members in your group right ....why aren't they running it and letting you know. The more you come on the forum the more seems to be you are sourcing.

 

[Ultimatepip]

Didn't he say free gear??..

 

[Ultimatepip]

Sounds like he wants others opinion on the cyp

 

[Ourlegacy]

Damn, I just read this and things keep getting worse haha.

Has anything mass spec'd out correctly lately ? Everything I have seen has tested out awful. Plus All the gear I have had recently has been garbage.

Shamrock, in theory I think it's a great idea and I believe you have good intentions. I don't see it any different than using any of these new sources popping up every day. I guarantee that almost all of them are not set up in some lab somewhere brewing...... Just wish the powder was purer from the get go.

 

[Ourlegacy]

Oh, what was the source for the raws ? So we all know you to avoid.

 

[shamrockbear1]

Yes I have a couple of guys but I brewed quite a bit don't know what to do with hundreds of bottles lol so rather sell it fund my habit just being honest ...

 

[clamz]

I'm not busting balls to be a dick, i just would like that you be careful with it. I'm not sure how your running it painless, that would be a miracle. its 1000 mg/ml of powder, only 250 of which is the correct compound and one with a high melt temp at that. i got teest cyp powder mass specd last year at 70% so i brewed it with gso/ba/bb at 330 mg/ml to compensate and the pip was horrrendous. next time i used 30% eo and it was just a day or two of discomfort. Even test e at 350mg/ml with no eo sucks pretty bad. Your basically running 75% garbage 25% cyp. you can't really compaarre it with a ugl with 60% purity becausse they only would have to use 3.5 grams per 10 ml which is a far cry from 10. Even if they didn't id still rather run 2ml of that stuff than a ml of 25% dosed cyp compensated by adding 7.5 extra grams of powder, that is insanely dangerous. in any case if its 25% pure i doubt their work ethic and dilligence towards sterility in the manufacturing process.

 

[shamrockbear1]

It is what it is so at least its properly dosed and 10 grams of powder is miniscule. Have no pip what's so ever using gso and smooth as silk... Now you know that pip is not an indicator of much many people get pip for all types of reasons