MESO-Rx
Anabolic Steroids
How do you filter castor oil?
- Thread starter Sampei
- Start date
that will murder my stopper tho
I'll probably use a vial spike or just a normal hypodermic needle with a filter.
Dirthand
Well-known Member
i have found that the silicone septas that i have do not take well to any kind of piercing either. I have pretty much counted them off as a one and done.that will murder my stopper tho
Is it only silicone? Only silicone is not good. You need silicone on one side and PTFE coated on the other side. That can take multiple piercing.
Dirthand
Well-known Member
It's the ptfe coated ones .. I get them from medlabsupply. ...
Strange it should be able to take multiple puncturing. What needle size are you using?
Dirthand
Well-known Member
Mostly 22gauge.. sometimes the hole seals up sometimes they don't very well.. since I filter againg with syringe filters when I transfer i don't even use the septas anymore. I try and vial up everything I make.. and if I can't i just put a regular cap on it to store. My process may change in the future.... to gear more twards cutting out syringe filtering all together like you are wanting to do. !
Dirthand
Well-known Member
I would like to add that i have 2 more samples in the process of being tested for sterility.. so far 10 days NG (no growth) i am sending 2 more off tommarrow.. as the test keeo coming back sterile I will start feeling more comfortable with different methods..
Use smaller gauge like 25G. You can draw no problem with 25G
Ghoul
Well-known Member
TLDR: If an average of 6% of compounds transferred between containers in a pharmacy ISO clean room introduce microbial contamination there's no way that's not happening in home environment. It's 0% with a hood.
Don't mistake the "I injected it and it didn't kill me" with free from bacteria. Most bacterial infections are asymptomatic, but can go on to damage joints (reactive arthritis) and organs, incrementally, over time.
It's up to the individual and their risk tolerance, but a hood is far from useless for those who want the lowest risk of long term harm from contaminated injectables.
As far as Jano's sterility testing, the focus is on the wrong thing imo. "Sterility" means an environment where bacteria can't reproduce. It doesn't mean your vial, made with non sterile ingredients in a non sterile environment, isn't packed with bacteria, that, once injected are no longer prevented from reproducing by benzyl alcohol. It's your immune system that's the final filter. A LAL test is required to measure the presence of (the worst) microbes (which also measures endotoxin, and combined are called the "bioburden").
"A study evaluating the incidence of microbial contamination of CSPs evaluated 3 testing environments: an ISO class 5 horizontal laminar-airflow hood in an ISO class 6 cleanroom, an ISO class 7 drug preparation area in an operating room, and an uncontrolled decentralized pharmacy on a ward. The authors compared 4 common compounding manipulations and identified overall contamination rates of 0%, 6%, and 16% after 14 days for syringes prepared in the cleanroom, operating room, and ward respectively."
www.pharmacytimes.com
Don't mistake the "I injected it and it didn't kill me" with free from bacteria. Most bacterial infections are asymptomatic, but can go on to damage joints (reactive arthritis) and organs, incrementally, over time.
It's up to the individual and their risk tolerance, but a hood is far from useless for those who want the lowest risk of long term harm from contaminated injectables.
As far as Jano's sterility testing, the focus is on the wrong thing imo. "Sterility" means an environment where bacteria can't reproduce. It doesn't mean your vial, made with non sterile ingredients in a non sterile environment, isn't packed with bacteria, that, once injected are no longer prevented from reproducing by benzyl alcohol. It's your immune system that's the final filter. A LAL test is required to measure the presence of (the worst) microbes (which also measures endotoxin, and combined are called the "bioburden").
"A study evaluating the incidence of microbial contamination of CSPs evaluated 3 testing environments: an ISO class 5 horizontal laminar-airflow hood in an ISO class 6 cleanroom, an ISO class 7 drug preparation area in an operating room, and an uncontrolled decentralized pharmacy on a ward. The authors compared 4 common compounding manipulations and identified overall contamination rates of 0%, 6%, and 16% after 14 days for syringes prepared in the cleanroom, operating room, and ward respectively."
Outside the Hood
Pharmacy Times offers the latest news and insights for the pharmacy professional and solutions that impact the everyday practice of pharmacy.
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narta
Member
Take your mothafucking fear mongering bs somewhere else hombre.
If you transfer from a sterile container to another sterile container using a sterile vehicle between the two I don't see how any contamination can happen.TLDR: If an average of 6% of compounds transferred between containers in a pharmacy ISO clean room introduce microbial contamination there's no way that's not happening in home environment. It's 0% with a hood.
Don't mistake the "I injected it and it didn't kill me" with free from bacteria. Most bacterial infections are asymptomatic, but can go on to damage joints (reactive arthritis) and organs, incrementally, over time.
It's up to the individual and their risk tolerance, but a hood is far from useless for those who want the lowest risk of long term harm from contaminated injectables.
As far as Jano's sterility testing, the focus is on the wrong thing imo. "Sterility" means an environment where bacteria can't reproduce. It doesn't mean your vial, made with non sterile ingredients in a non sterile environment, isn't packed with bacteria, that, once injected are no longer prevented from reproducing by benzyl alcohol. It's your immune system that's the final filter. A LAL test is required to measure the presence of (the worst) microbes (which also measures endotoxin, and combined are called the "bioburden").
"A study evaluating the incidence of microbial contamination of CSPs evaluated 3 testing environments: an ISO class 5 horizontal laminar-airflow hood in an ISO class 6 cleanroom, an ISO class 7 drug preparation area in an operating room, and an uncontrolled decentralized pharmacy on a ward. The authors compared 4 common compounding manipulations and identified overall contamination rates of 0%, 6%, and 16% after 14 days for syringes prepared in the cleanroom, operating room, and ward respectively."
Outside the Hood
Pharmacy Times offers the latest news and insights for the pharmacy professional and solutions that impact the everyday practice of pharmacy.
If I draw with a sterile syringe from a sterile media bottle and inject into a sterile sealed vial how can it get contaminated? Magic?
Ghoul
Well-known Member
Every time a vial is penetrated in a non sterile environment microbes are introduced. That's why multi-dose vials have to have BA, and ampules don't. Once a sealed ampule is (heat or radiation) sterilized it stays that way.
From the stopper top, the unsterile air in the syringe. While this study was done in a hospital, every environment is full of bacteria. They're using sterile needles, sterile pharma product, and presumably sterilizing the vial tops yet significant levels of contamination are found in multi-dose vials.
My comment was more about filling open top vials without a hood, which is the worst case scenario, but even needle transfer in open air causes it. That's why Testosterone and other pharma injectables may have a multi-year shelf life, but instruct you to use them within a month of first penetration of the vial.
Bacterial contamination of single and multiple-dose parenteral injection vials after opening and antibiotic susceptibility of isolates at Jimma Medical Center, Jimma, Southwest Ethiopia - PMC
Single- or multiple-dose vials are prone to bacterial contamination after improper handling and can be potential reservoirs of microorganisms that could be transmitted to the patient through the parenteral route. The present study aims to assess the ...
pmc.ncbi.nlm.nih.gov
Again, not everyone cares, nor do they have to. But it's good to know you can't usually "feel" bacteria, that they can hurt you over many years, and what it takes to reduce them, for anyone who wants to go to the next level.
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Dirthand
Well-known Member
No 25 gauge does not draw worth a fuck for filling up a 60 or 100ml syringe.. 22 gauge is really kind of depressing as well.. I have some w 16 gauge that works like a charm.... I just screw the top off use those..
Mr. Sampi i wasn't like that at first but after doing some testing and brewing g and injecting.. you will possibly lax just a tad as well . Maybe not thought!! I know that you will be fucking great either way my dawg!!!!
D
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Now I understand better and yeah I completely understand what you are saying.Every time a vial is penetrated in a non sterile environment microbes are introduced. That's why multi-dose vials have to have BA, and ampules don't. Once a sealed ampule is (heat or radiation) sterilized it stays that way.
From the stopper top, the unsterile air in the syringe. While this study was done in a hospital, every environment is full of bacteria. They're using sterile needles, sterile pharma product, and presumably sterilizing the vial tops yet significant levels of contamination are found in multi-dose vials.
My comment was more about filling open top vials without a hood, which is the worst case scenario, but even needle transfer in open air causes it. That's why Testosterone and other pharma injectables may have a multi-year shelf life, but instruct you to use them within a month of first penetration of the vial.
Bacterial contamination of single and multiple-dose parenteral injection vials after opening and antibiotic susceptibility of isolates at Jimma Medical Center, Jimma, Southwest Ethiopia - PMC
Single- or multiple-dose vials are prone to bacterial contamination after improper handling and can be potential reservoirs of microorganisms that could be transmitted to the patient through the parenteral route. The present study aims to assess the ...
Again, not everyone cares, nor do they have to. But it's good to know you can't usually "feel" bacteria, that they can hurt you over many years, and what it takes to reduce them, for anyone who wants to go to the next level.
knucklesamich
New Member
I use a very similar method, except I use 50 ml vials. If at some point in time, it looks like the stopper is getting too used/abused, I just rip off the whole lid and replace it with new rubber stopper etc--it usually doesn't happen though.
You and I may be a little overboard LOL. I got one of those air purifiers too. But my overthinking brain was thinking isnt it better to turn it off before brewing so the air isnt moving?
You don't keep it close to your brewing station when you are doing sensible thing so it doesn't really matter, I guess.
You keep the air clean if you keep it running even so it is already clean if you have used it in a closed room for like 1 hour so... I believe on or off doesn't really matter.
Are you capping your own vial? If no you don't need to worry about air moving really.
If you are capping your vial and you wanna go overboard and I have been assured by ppl that have done this for 20+ years IT IS NOT NEEDED, so let it be clear it's just for your own personal peace of mind and being like me autistic 100%
I have a better little thing to use while you are capping it.
Laminar Flow Hood - HEPA filter, Mycology works with high success rate
Discover the secret to clean mushroom cultures with our custom 200x255 laminar flow hood, specially designed for optimal mushroom cultivation.
urbanfungi.ee
Again not needed, not needed, not needed but very cool and not really expensive
That little flow hood is awesome!!!!! I refilter in sterile vials BUT then after pop the cap and put a new stopper and cap. Again overboard but I feel betterYou don't keep it close to your brewing station when you are doing sensible thing so it doesn't really matter, I guess.
You keep the air clean if you keep it running even so it is already clean if you have used it in a closed room for like 1 hour so... I believe on or off doesn't really matter.
Are you capping your own vial? If no you don't need to worry about air moving really.
If you are capping your vial and you wanna go overboard and I have been assured by ppl that have done this for 20+ years IT IS NOT NEEDED, so let it be clear it's just for your own personal peace of mind and being like me autistic 100%
I have a better little thing to use while you are capping it.
Laminar Flow Hood - HEPA filter, Mycology works with high success rate
Discover the secret to clean mushroom cultures with our custom 200x255 laminar flow hood, specially designed for optimal mushroom cultivation.
Again not needed, not needed, not needed but very cool and not really expensive
