However that's not what is worrisome at all since these devices (MS and Chromatography) typically have different type sets bc the numeric distribution, collation must accommodate the varied graphs required for interpretation.
No so what is the problem? The MASS SPEC simply does not "fit" the 100% HPLC purity result. That's even considering the algorithmic possibility of an Oxygen adducts, Polycyclic testosterone based Aggregates, Dimers or Isotopes
The bottom line, even when the adducts, aggregates, monomers or dimers are accounted for the MS fragmentation pattern is not at all consistent with a purity of 100%
So what is it's purity?
Really there is no way to make that determination without repeating the HPLC. The test should include at least two dilutions (usually a 0.25% and 1.25) to detect any optical variance which may have accounted for some of the discrepancies.
However the MS may also need to be repeated bc the MW of MANY contaminant AAS can skew HPLC purity data remarkably. (For example substituting a AAS molecular
Those are my suggestions Capt
jim