The problem is that people are relying on one persons lab for HCG testing (janoshik) and as a matter of fact Janoshik is a very smart man who i have a lot of respect for. You may be unaware of it but this same issue has come up with Janoshik before and he has had discourse with a European HCG manufacturer who outlined exactly why he believes Janoshik's method of testing HCG is not accurate, (many many vendors have their HCG results come back half dosed or severely underdosed.) Even QDS had his HCG come back half dosed from Janoshik.
So either there is a giant conspiracy on to underdose a peptide, or Janoshik's testing
for HCG is flawed. I will link Janoshik's thread here
HCG discourse
it is a long read but well worth it to hear from an actual manufacturer of HCG. Here is one quote for the people who won't read all of the thread.
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I want to get back to you regarding your HCG testing, I spoke to other HCG producers who produce it using chinese HCG raw material, also to some other big sellers of HCG who import HCG vials, they all get same lower results than the expected 5000IU, and results range in 3000-3500IU which is a very low result.
As an example I sent vial from same batch, which you tested a bit above 3000IU to Analiza Bialek lab, and they showed correct results of nearly 6000IU. Check attachment.
You should really recheck how you test HCG and what standard you use, because this problem I see is not only with my production HCG but its mainstream problem which you have which affects HCG sales, with your current testing everyone has to add 10000IU so you would show 5000IU in your testing.
I am not pointing to your oils testing or your HGH testing, I am specifically saying about your HCG testing.''
''Regarding why no-one us using HPLC method in pharmacopeia you are not correct, as HPLC would be much cheaper and easier method to determine HCG bioactivity if it was possible, everyone would use it, if it would be possible to validate it for determination of HCG bioactivity in production or testing purposes, but no-one uses it because it is not suitable method for this particular protein, due to huge variation in glycolisation pattern and that pattern has big influence to bioactivity, so one protein with the same amount of mg/ml with one glycolisation pattern can have very different bioactivity with the same amount mg/ml with different glycolisation pattern.
I would not agree that no one is using hplc in pharmacopoeia, for lot's of proteins HPLC validation is the best and most important step in determination of protein characteristics, but not all proteins are the same. And in this regard you can not measure everything using the same scheme.''