Readalots Enhanced Testing

[readalot]

How are compounds tested for purity that go to the compounding pharmacies. Do the same.

It’s HPLC testing.

Let @janoshik know so he can quit the research. @BigNoOne already know that -ene impurities of Testosterone esters missing a proton on two adjacent carbon atoms in the gonane ring are properly resolved on his HPLC setup and not counted in the API peak. You could have saved him 200 USD on the reference standard he just purchased. He could use an analytical chemistry guru such as yourself.

All "HPLC" is the same. Got it.

 

[readalot]

PROJECT 2 coming soon.

Raws HPLC testing, metals, and endotoxins.

Focus was on the raws but we got some finished oil vials and lyophilized GH vial in there as well.

I want to thank @Sampei very much for his collaboration on this project. His contributions to further our body of knowledge as a community is simply incredible. He donated the 9 raws, finished oil vials, and a lyophilized GH vial. He did all the heavy lifting and got the samples to Jano. We went in half on the HPLC testing and I covered the remainder of the tests.

Quote number #xxxxx :
-------------------------
9x Blind common anabolic steroid and aromatase inhibitors screening - raws x 120 USD MINUS 10% DISCOUNT= 972 USD

6x Heavy metals screening (full EDX panel) x 60 USD MINUS 10% DISCOUNT= 324 USD

5x Endotoxin analysis x 120 USD MINUS 10% DISCOUNT= 540 USD

1x Endotoxin on HGH analysis x 120 USD MINUS 10% DISCOUNT= 108 USD

1x Endotoxin on AAS oil analysis x 120 USD MINUS 10% DISCOUNT= 108 USD

1x Heavy metals screening (full EDX panel) for oil sample x 60 USD MINUS 10% DISCOUNT= 54 USD

9x Raw data x 5 USD = 45 USD
-------------------------
TOTAL: 2151 USD

Project 2 Results

The community owes @Sampei a sincere thanks for coordinating the testing and sending all the samples. We split the cost on HPLC, and I covered the remainder of tests. A good start to demonstrate product surveillance in action for the tests other than HPLC.

HPLC on raws
[ @SigmaAudley China Peptide ]

Raw data files provided on Janoshik's website.

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Endotoxin analysis on raws
[ @SigmaAudley China Peptide ]

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Endotoxin analysis on finished oil
[ @SigmaAudley China Peptide ]

Janoshik Analytical

Endotoxin analysis on GH
[ @Qingdao Sigma Chemicals ]

Janoshik Analytical

Summary table for endotoxin analysis provided by Janoshik


Metals analysis on raws (raw data included)
[ @SigmaAudley China Peptide ]

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Metals analysis on finished oil (raw data included) [ @GenericAsia ]

Janoshik Analytical

EDX raw data for all metals is included as pdf on the website.

 

[readalot]

Project 2 HPLC Raws Reports

 

[readalot]

Project 2 Endotoxin Reports

 

[readalot]

Project 2 Metals Reports

 

[Dkland]

To my uneducated eye, and simple mind…..those endotoxin results look good. Am I correct in that assumption?
I don’t know what the purity of raws should be. How do those stack up as far as what was expected?

Thank you guys for doing this.

 

[Vanargandar]

From temp-ban, to one of the most valuable assets on the forum. What a rise to power.

 

[SigmaAudley China Peptide]

Project 2 Results

The community owes @Sampei a sincere thanks for coordinating the testing and sending all the samples. We split the cost on HPLC, and I covered the remainder of tests. A good start to demonstrate product surveillance in action for the tests other than HPLC.

HPLC on raws
[ @SigmaAudley China Peptide ]

Raw data files provided on Janoshik's website.

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Endotoxin analysis on raws
[ @SigmaAudley China Peptide ]

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Endotoxin analysis on finished oil
[ @SigmaAudley China Peptide ]

Janoshik Analytical

Endotoxin analysis on GH
[ @Qingdao Sigma Chemicals ]

Janoshik Analytical

Summary table for endotoxin analysis provided by Janoshik
View attachment 303794

Metals analysis on raws (raw data included)
[ @SigmaAudley China Peptide ]

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Janoshik Analytical

Metals analysis on finished oil (raw data included) [ @GenericAsia ]

Janoshik Analytical

EDX raw data for all metals is included as pdf on the website.

I would like to express my sincere thanks

 

[BigNoOne]

Endotoxins - fine

Heavy metals - not detected

It’s almost as if those are never in our stuff. Are we still going to test for all that when it’s NOT a concern?

 

[readalot]

Got a nice threat today. Very creative. I seem to have gotten someone's attention now. Won't lie. Didn't feel great reading it.

Anyway....

Project 1 (BU raw) full reports and raw data below. Thank you again for all your efforts to get this sample tested @Dirthand.

1. HPLC

Janoshik Analytical

2. GCMS

Janoshik Analytical

3. Metals by EDX

Janoshik Analytical

4. Endotoxins

Janoshik Analytical

The xxxx rows are peptide samples previously tested and presented for comparison (thanks @janoshik!).

5a. KF titration

Janoshik Analytical

5b. Loss on drying (LOD)

Janoshik Analytical

KF and LOD are symbolic tests. The fatty acid methyl ester came off during the LOD test so impossible to get at residual solvents using KF plus LOD. Very nice LOD setup Jano!

Project 1 Report Images

@Millard: do you want all the raw data pdfs uploaded as well or just the main report images?

 

[iris]

Thank you Readalot and @Sampei for making these results available to members and for shining a light on testing possibilities.

Your indefatigable research into AAS (whether related to compounds, testing, homebrewing and sources surveillance) is the biggest asset this forum has.
You make this place the enlightened, inspiring and innovative resource that it is.

❤

 

[readalot]

It’s almost as if those are never in our stuff. Are we still going to test for all that when it’s NOT a concern?

We (you and I) have gone around and around on this a few times now. Would you like me to give you my opinion again, or are you looking for opinions / discussion from other members? I hope the later.

I also hope your question stimulates broad discussion. Thanks for asking it again now that Results from Projects 1 and 2 are back.

I've demonstrated some of the testing on a small number of samples with help from collaborators. The community must now decide how to move forward and whether they will support and embrace a higher standard of product quality testing. The scope of what that may be will be interesting to watch play out.

I've started a database of vendor activity in this area here...

Thread 'Enhanced Testing Database'

Nov 14, 2024

Thread to track all of the great vendor / member activities in the enhanced testing arena. The other thread covers only Projects 1 - 3 (demonstration); vendor/member activity can be found.

Slowly a surveillance database for raw AAS and GH quality/impurities can be built for additional testing (additional to API purity, HPLC, etc ). If nothing is found then that's a great result to track as well. The data will be available for those interested.

Scope:

1. Hplc Purity
2. GCMS
3. Expanded metals list
4. Endotoxins
5. Residual solvents
6. Sterility

• readalot
• analytical endotoxins gcms hplc metals sterility testing reports
• Replies: 69
• Forum: Analytical Lab Testing

• 
• 

I'd appreciate vendors and members helping me track who has done what. Thank you.

 

[readalot]

To my uneducated eye, and simple mind…..those endotoxin results look good. Am I correct in that assumption?

Yes, all the endotoxin results from Project 2 (raws and 1 oil) look good.

I don’t know what the purity of raws should be. How do those stack up as far as what was expected?

I'll let @Sampei weigh in with his thoughts. I'll do a separate post comparing what's on the report vs the calculated values from the raw data.

Thank you guys for doing this.

You are most welcome.

 

[readalot]

Thank you Readalot and @Sampei for making these results available to members and for shining a light on testing possibilities.

Your indefatigable research into AAS (whether related to compounds, testing, homebrewing and sources surveillance) is the biggest asset this forum has.
You make this place the enlightened, inspiring and innovative resource that it is.

❤

Thank you for kind words. And Project 1 wasn't possible without Brother @Dirthand. Just to make it clear to future readers. He also kindly set up this thread while I was in "time-out".

Thanks for all the insight and brains you contribute here Iris.

 

[iris]

Thank you for kind words.

Thanks for all the insight and brains you contribute here Iris.

The kind words are fully deserved, and some.

Your words towards me, though, are always too generous.
Me = brains not really; enthusiasm = on a good day, lol.
But I recognise talent and intellect, when I see it.

You, Sir, not only have a brilliant intellect but also a kind and magnanimous heart.
A brave and honourable man that I am happy to have encountered.
Thank you for your time, your help and advice, always given selflessly.

 

[BigNoOne]

We (you and I) have gone around and around on this a few times now. Would you like me to give you my opinion again, or are you looking for opinions / discussion from other members?

I hope your question stimulates broad discussion. Thanks for asking it again now that Results from Projects 1 and 2 are back.

We all knew the heavy metals weren’t in there from the AnabolicLabs project years ago. And it came up several times. Jano had weighed in to indicate that he stopped marketing that analysis because he never detected heavy metals.

Why did you get so obsessed with it?

The endotoxins were new and that was a good one to evaluate. But it’s clear that they are not introduced as part of hormone manufacturing.

Just because our FDA says these things need to be analyzed doesn’t mean that they are or ever were a concern. The FDA is filled with bureaucratic bullshit and inefficiencies. Things that make no actual scientific sense.

Question for everyone though. Knowing that this stuff isn’t a concern, are you still willing to pay $5 more per vial for a cert that confirms this stuff that’s not a concern is in fact not in your vial?

 

[readalot]

We all knew the heavy metals weren’t in there from the AnabolicLabs project years ago. And it came up several times. Jano had weighed in to indicate that he stopped marketing that analysis because he never detected heavy metals.

Why did you get so obsessed with it?

In case your question is sincere:

Jan 2024:

Agreed on the heavy metals. I don't think that is a major concern but always good to trust but verify. The larger part of this (impurity ID) has not been properly discussed or analyzed. Hence my questions

The "obsession" angle never came from me but from those trying to ridicule and warp my inquiry.

The endotoxins were new and that was a good one to evaluate. But it’s clear that they are not introduced as part of hormone manufacturing.

How did you reach this conclusion? Its misleading. As part of raws synthesis? Correct.

As part of what should be sterile product manufacturing process? Only as good as the lab and its dead bacterial load. I don't agree with your conclusion and neither of us have the proper sample size to firmly make that conclusion. Do you comprehend what that would be compared to what I've shown here?

The rest I hope you get lots of feedback on. If you don't then you know the answer...the work is done and the idea is dead.

Thanks.

Just because our FDA says these things need to be analyzed doesn’t mean that they are or ever were a concern. The FDA is filled with bureaucratic bullshit and inefficiencies. Things that make no actual scientific sense.

Question for everyone though. Knowing that this stuff isn’t a concern, are you still willing to pay $5 more per vial for a cert that confirms this stuff that’s not a concern is in fact not in your vial?

I do want to plug the finale of Project 3. Hope to see @janoshik 's results on the HPLC vs GCMS purity of Test E/Test C raws and the impact of certain Testosterone impurities on the accuracy of HPLC purity.

 

[iris]

We all knew the heavy metals weren’t in there from the AnabolicLabs project years ago. And it came up several times. Jano had weighed in to indicate that he stopped marketing that analysis because he never detected heavy metals.

Why did you get so obsessed with it?

The endotoxins were new and that was a good one to evaluate. But it’s clear that they are not introduced as part of hormone manufacturing.

Just because our FDA says these things need to be analyzed doesn’t mean that they are or ever were a concern. The FDA is filled with bureaucratic bullshit and inefficiencies. Things that make no actual scientific sense.

Question for everyone though. Knowing that this stuff isn’t a concern, are you still willing to pay $5 more per vial for a cert that confirms this stuff that’s not a concern is in fact not in your vial?

Since I saw you on a source thread or two, what are your requirements for every vendor that is on the forum?
What should sources provide, on a continual basis, for the raw and finished products they offer?

 

[readalot]

From temp-ban, to one of the most valuable assets on the forum. What a rise to power.

I appreciate your kind words Brother.

Power? Haha, I got none. Just persistent and wanted to demonstrate a new way of doing things. I'm excited that there will be some new options in this paradigm coming soon for the UGL customers. Some vendors will embrace it (it appears).

I hope folks will share their thoughts and preferences. I hope the info I have shared is educational and useful to the community.

 

[BigNoOne]

Since I saw you on a source thread or two, what are your requirements for every vendor that is on the forum?
What should sources provide, on a continual basis, for the raw and finished products they offer?

I believe in testing. As long as it’s reasonable. My fear with the “enhanced” testing is that it creates a “nothing is good enough for MESO” environment amongst sources. Sources could snub the testing altogether. It was only a few years ago that HPLC testing demand across all sources was popularized here and then on other forums.

If the “enhanced” testing identified something concerning then my stance would be much different. But it’s clear to me that none of these analyses are “protective” in that there’s nothing there to begin with.