MESO-Rx

Anabolic Steroids

Readalots Enhanced Testing

BigNoOne said:
How are compounds tested for purity that go to the compounding pharmacies. Do the same.

It’s HPLC testing.
Click to expand...
Let @janoshik know so he can quit the research. @BigNoOne already know that -ene impurities of Testosterone esters missing a proton on two adjacent carbon atoms in the gonane ring are properly resolved on his HPLC setup and not counted in the API peak. You could have saved him 200 USD on the reference standard he just purchased. He could use an analytical chemistry guru such as yourself.

All "HPLC" is the same. Got it. ;)
 
Last edited:
readalot said:
PROJECT 2 coming soon.

Raws HPLC testing, metals, and endotoxins.

Focus was on the raws but we got some finished oil vials and lyophilized GH vial in there as well.

I want to thank @Sampei very much for his collaboration on this project. His contributions to further our body of knowledge as a community is simply incredible. He donated the 9 raws, finished oil vials, and a lyophilized GH vial. He did all the heavy lifting and got the samples to Jano. We went in half on the HPLC testing and I covered the remainder of the tests.

Quote number #xxxxx :
-------------------------
9x Blind common anabolic steroid and aromatase inhibitors screening - raws x 120 USD MINUS 10% DISCOUNT= 972 USD

6x Heavy metals screening (full EDX panel) x 60 USD MINUS 10% DISCOUNT= 324 USD

5x Endotoxin analysis x 120 USD MINUS 10% DISCOUNT= 540 USD

1x Endotoxin on HGH analysis x 120 USD MINUS 10% DISCOUNT= 108 USD

1x Endotoxin on AAS oil analysis x 120 USD MINUS 10% DISCOUNT= 108 USD

1x Heavy metals screening (full EDX panel) for oil sample x 60 USD MINUS 10% DISCOUNT= 54 USD

9x Raw data x 5 USD = 45 USD
-------------------------
TOTAL: 2151 USD
Click to expand...

Project 2 Results

The community owes @Sampei a sincere thanks for coordinating the testing and sending all the samples. We split the cost on HPLC and I covered the remainder of tests. A good start to demonstrate product surveillance in action of the tests other than HPLC.

HPLC on raws
[ @SigmaAudley China Peptide ]











Endotoxin analysis on raws
[ @SigmaAudley China Peptide ]







Endotoxin analysis on finished oil
[ @SigmaAudley China Peptide ]


Endotoxin analysis on GH
[ @Qingdao Sigma Chemicals ]


Metals analysis on raws (raw data included)
[ @SigmaAudley China Peptide ]







Metals analysis on finished oil (raw data included) [ @GenericAsia ]

 
readalot said:
PROJECT 2 coming soon.

Raws HPLC testing, metals, and endotoxins.

Focus was on the raws but we got some finished oil vials and lyophilized GH vial in there as well.

I want to thank @Sampei very much for his collaboration on this project. His contributions to further our body of knowledge as a community is simply incredible. He donated the 9 raws, finished oil vials, and a lyophilized GH vial. He did all the heavy lifting and got the samples to Jano. We went in half on the HPLC testing and I covered the remainder of the tests.

Quote number #xxxxx :
-------------------------
9x Blind common anabolic steroid and aromatase inhibitors screening - raws x 120 USD MINUS 10% DISCOUNT= 972 USD

6x Heavy metals screening (full EDX panel) x 60 USD MINUS 10% DISCOUNT= 324 USD

5x Endotoxin analysis x 120 USD MINUS 10% DISCOUNT= 540 USD

1x Endotoxin on HGH analysis x 120 USD MINUS 10% DISCOUNT= 108 USD

1x Endotoxin on AAS oil analysis x 120 USD MINUS 10% DISCOUNT= 108 USD

1x Heavy metals screening (full EDX panel) for oil sample x 60 USD MINUS 10% DISCOUNT= 54 USD

9x Raw data x 5 USD = 45 USD
-------------------------
TOTAL: 2151 USD
Click to expand...

Project 2 Results

The community owes @Sampei a sincere thanks for coordinating the testing and sending all the samples. We split the cost on HPLC, and I covered the remainder of tests. A good start to demonstrate product surveillance in action for the tests other than HPLC.

HPLC on raws
[ @SigmaAudley China Peptide ]

Raw data files provided on Janoshik's website.











Endotoxin analysis on raws
[ @SigmaAudley China Peptide ]







Endotoxin analysis on finished oil
[ @SigmaAudley China Peptide ]


Endotoxin analysis on GH
[ @Qingdao Sigma Chemicals ]



Summary table for endotoxin analysis provided by Janoshik
image (13).webp

Metals analysis on raws (raw data included)
[ @SigmaAudley China Peptide ]







Metals analysis on finished oil (raw data included) [ @GenericAsia ]


EDX raw data for all metals is included as pdf on the website.
 
Last edited:
Project 2 HPLC Raws Reports
 

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Project 2 Endotoxin Reports
 

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Project 2 Metals Reports
 

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To my uneducated eye, and simple mind…..those endotoxin results look good. Am I correct in that assumption?
I don’t know what the purity of raws should be. How do those stack up as far as what was expected?

Thank you guys for doing this.
 
readalot said:
Project 2 Results

The community owes @Sampei a sincere thanks for coordinating the testing and sending all the samples. We split the cost on HPLC and I covered the remainder of tests. A good start to demonstrate product surveillance in action of the tests other than HPLC.

HPLC on raws
[ @SigmaAudley China Peptide ]











Endotoxin analysis on raws
[ @SigmaAudley China Peptide ]







Endotoxin analysis on finished oil
[ @SigmaAudley China Peptide ]


Endotoxin analysis on GH
[ @Qingdao Sigma Chemicals ]


Metals analysis on raws (raw data included)
[ @SigmaAudley China Peptide ]







Metals analysis on finished oil (raw data included) [ @GenericAsia ]

Click to expand...
Can you delete this post #422 @Millard ? Posted twice for some reason.

#423 is revised with additional text so don't know how the edit posted as a new post.
 
Last edited:
readalot said:
Project 2 Results

The community owes @Sampei a sincere thanks for coordinating the testing and sending all the samples. We split the cost on HPLC, and I covered the remainder of tests. A good start to demonstrate product surveillance in action for the tests other than HPLC.

HPLC on raws
[ @SigmaAudley China Peptide ]

Raw data files provided on Janoshik's website.











Endotoxin analysis on raws
[ @SigmaAudley China Peptide ]







Endotoxin analysis on finished oil
[ @SigmaAudley China Peptide ]


Endotoxin analysis on GH
[ @Qingdao Sigma Chemicals ]



Summary table for endotoxin analysis provided by Janoshik
View attachment 303794

Metals analysis on raws (raw data included)
[ @SigmaAudley China Peptide ]







Metals analysis on finished oil (raw data included) [ @GenericAsia ]


EDX raw data for all metals is included as pdf on the website.
Click to expand...
I would like to express my sincere thanks
 
Endotoxins - fine

Heavy metals - not detected

It’s almost as if those are never in our stuff. Are we still going to test for all that when it’s NOT a concern?
 
readalot said:
Got a nice threat today. Very creative. I seem to have gotten someone's attention now. Won't lie. Didn't feel great reading it.

Anyway....

Project 1 (BU raw) full reports and raw data below. Thank you again for all your efforts to get this sample tested @Dirthand.

1. HPLC

2. GCMS

3. Metals by EDX

4. Endotoxins

The xxxx rows are peptide samples previously tested and presented for comparison (thanks @janoshik!).

5a. KF titration

5b. Loss on drying (LOD)

KF and LOD are symbolic tests. The fatty acid methyl ester came off during the LOD test so impossible to get at residual solvents using KF plus LOD. Very nice LOD setup Jano!
Click to expand...
Project 1 Report Images

@Millard: do you want all the raw data pdfs uploaded as well or just the main report images?
 

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Thank you Readalot and @Sampei for making these results available to members and for shining a light on testing possibilities.

Your indefatigable research into AAS (whether related to compounds, testing, homebrewing and sources surveillance) is the biggest asset this forum has.
You make this place the enlightened, inspiring and innovative resource that it is.

❤
 
BigNoOne said:
It’s almost as if those are never in our stuff. Are we still going to test for all that when it’s NOT a concern?
Click to expand...
We (you and I) have gone around and around on this a few times now. Would you like me to give you my opinion again, or are you looking for opinions / discussion from other members? I hope the later.

I also hope your question stimulates broad discussion. Thanks for asking it again now that Results from Projects 1 and 2 are back.

I've demonstrated some of the testing on a small number of samples with help from collaborators. The community must now decide how to move forward and whether they will support and embrace a higher standard of product quality testing. The scope of what that may be will be interesting to watch play out.

I've started a database of vendor activity in this area here...

readalot

Thread 'Enhanced Testing Database'

Thread to track all of the great vendor / member activities in the enhanced testing arena. The other thread covers only Projects 1 - 3 (demonstration); vendor/member activity can be found.

Slowly a surveillance database for raw AAS and GH quality/impurities can be built for additional testing (additional to API purity, HPLC, etc ). If nothing is found then that's a great result to track as well. The data will be available for those interested.

Scope:

1. Hplc Purity
2. GCMS
3. Expanded metals list
4. Endotoxins
5. Residual solvents
6. Sterility
  • Like
  • Love

I'd appreciate vendors and members helping me track who has done what. Thank you.
 
Last edited:
Dkland said:
To my uneducated eye, and simple mind…..those endotoxin results look good. Am I correct in that assumption?
Click to expand...
Yes, all the endotoxin results from Project 2 (raws and 1 oil) look good.


Dkland said:
I don’t know what the purity of raws should be. How do those stack up as far as what was expected?
Click to expand...

I'll let @Sampei weigh in with his thoughts. I'll do a separate post comparing what's on the report vs the calculated values from the raw data.

Dkland said:
Thank you guys for doing this.
Click to expand...
You are most welcome.
 
iris said:
Thank you Readalot and @Sampei for making these results available to members and for shining a light on testing possibilities.

Your indefatigable research into AAS (whether related to compounds, testing, homebrewing and sources surveillance) is the biggest asset this forum has.
You make this place the enlightened, inspiring and innovative resource that it is.

❤
Click to expand...
Thank you for kind words. And Project 1 wasn't possible without Brother @Dirthand. Just to make it clear to future readers. He also kindly set up this thread while I was in "time-out".

Thanks for all the insight and brains you contribute here Iris.
 
readalot said:
Thank you for kind words.

Thanks for all the insight and brains you contribute here Iris.
Click to expand...

The kind words are fully deserved, and some.

Your words towards me, though, are always too generous.
Me = brains not really; enthusiasm = on a good day, lol.
But I recognise talent and intellect, when I see it.

You, Sir, not only have a brilliant intellect but also a kind and magnanimous heart.
A brave and honourable man that I am happy to have encountered.
Thank you for your time, your help and advice, always given selflessly.
 
readalot said:
We (you and I) have gone around and around on this a few times now. Would you like me to give you my opinion again, or are you looking for opinions / discussion from other members?

I hope your question stimulates broad discussion. Thanks for asking it again now that Results from Projects 1 and 2 are back.
Click to expand...
We all knew the heavy metals weren’t in there from the AnabolicLabs project years ago. And it came up several times. Jano had weighed in to indicate that he stopped marketing that analysis because he never detected heavy metals.

Why did you get so obsessed with it?

The endotoxins were new and that was a good one to evaluate. But it’s clear that they are not introduced as part of hormone manufacturing.

Just because our FDA says these things need to be analyzed doesn’t mean that they are or ever were a concern. The FDA is filled with bureaucratic bullshit and inefficiencies. Things that make no actual scientific sense.

Question for everyone though. Knowing that this stuff isn’t a concern, are you still willing to pay $5 more per vial for a cert that confirms this stuff that’s not a concern is in fact not in your vial?
 
BigNoOne said:
We all knew the heavy metals weren’t in there from the AnabolicLabs project years ago. And it came up several times. Jano had weighed in to indicate that he stopped marketing that analysis because he never detected heavy metals.

Why did you get so obsessed with it?
Click to expand...
In case your question is sincere:

Jan 2024:
readalot said:
Agreed on the heavy metals. I don't think that is a major concern but always good to trust but verify. The larger part of this (impurity ID) has not been properly discussed or analyzed. Hence my questions
Click to expand...

The "obsession" angle never came from me but from those trying to ridicule and warp my inquiry.

BigNoOne said:
The endotoxins were new and that was a good one to evaluate. But it’s clear that they are not introduced as part of hormone manufacturing.
Click to expand...

How did you reach this conclusion? Its misleading. As part of raws synthesis? Correct.

As part of what should be sterile product manufacturing process? Only as good as the lab and its dead bacterial load. I don't agree with your conclusion and neither of us have the proper sample size to firmly make that conclusion. Do you comprehend what that would be compared to what I've shown here?

The rest I hope you get lots of feedback on. If you don't then you know the answer...the work is done and the idea is dead.

Thanks.

BigNoOne said:
Just because our FDA says these things need to be analyzed doesn’t mean that they are or ever were a concern. The FDA is filled with bureaucratic bullshit and inefficiencies. Things that make no actual scientific sense.

Question for everyone though. Knowing that this stuff isn’t a concern, are you still willing to pay $5 more per vial for a cert that confirms this stuff that’s not a concern is in fact not in your vial?
Click to expand...

I do want to plug the finale of Project 3. Hope to see @janoshik 's results on the HPLC vs GCMS purity of Test E/Test C raws and the impact of certain Testosterone impurities on the accuracy of HPLC purity.
 
Last edited:
BigNoOne said:
We all knew the heavy metals weren’t in there from the AnabolicLabs project years ago. And it came up several times. Jano had weighed in to indicate that he stopped marketing that analysis because he never detected heavy metals.

Why did you get so obsessed with it?

The endotoxins were new and that was a good one to evaluate. But it’s clear that they are not introduced as part of hormone manufacturing.

Just because our FDA says these things need to be analyzed doesn’t mean that they are or ever were a concern. The FDA is filled with bureaucratic bullshit and inefficiencies. Things that make no actual scientific sense.

Question for everyone though. Knowing that this stuff isn’t a concern, are you still willing to pay $5 more per vial for a cert that confirms this stuff that’s not a concern is in fact not in your vial?
Click to expand...

Since I saw you on a source thread or two, what are your requirements for every vendor that is on the forum?
What should sources provide, on a continual basis, for the raw and finished products they offer?
 
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