janoshik
Subscriber
Yes I did just now google and I'm going with Pete Holt with Cambridge He's stating it's the gold standard as well. As well is many others.
mans
And I can go with Mr. Jeffrey Whiteaker, Ph.D., who is currently Director of Proteomics in the laboratory of Mandy Paulovich at the Fred Hutchinson Cancer Research Center in Seattle, WA, who states the same about ELISA in a *published article*
Also if you are referring to this thread: What is the most accurate method of determining protein concentration?
Please note quite important points:
1. unavailability of standard
2. it is not an article, just a post on the board
3. the guy you are referring to states "If you're dealing with a crude sample and want to determine the concentration of just one protein amongst many, you have to have an assay specific for that protein and pure protein to compare it to." WHICH IS NOT THE CASE WITH AAA!!!