Because I've grown weary of the entire process I'll only respond to one of Ks replies..
The absorbance of a compound is a product of it's elemental structure, concentration, media thickness, and the solvents used.
It's been well established proteins including DNA/RNA absorb UV light in the 280nm range. (DNA has optimal absorbance at 260nm)
But simply assuming the results are not dependent upon other factors listed, is in error.
Consequently to ensure the evaluation of a homogenous mixture of identical molecular densities (purity) is being evaluated an HPLC MUST be performed.
Bc IF the latter has NOT been conducted then the researcher is assuming a VERY high level of purity.
One could rightfully contend that impurities would cause a shift to the left or right, but if the mixture is homogeneous impurities of dissimilar structure and polarities may "cancel each other out" thus having a minimal effect of the OD of the mixture under study.
So to PROVE an absolute purity of X, Y, or Z chromatography is the standard.
Jim
The absorbance of a compound is a product of it's elemental structure, concentration, media thickness, and the solvents used.
It's been well established proteins including DNA/RNA absorb UV light in the 280nm range. (DNA has optimal absorbance at 260nm)
But simply assuming the results are not dependent upon other factors listed, is in error.
Consequently to ensure the evaluation of a homogenous mixture of identical molecular densities (purity) is being evaluated an HPLC MUST be performed.
Bc IF the latter has NOT been conducted then the researcher is assuming a VERY high level of purity.
One could rightfully contend that impurities would cause a shift to the left or right, but if the mixture is homogeneous impurities of dissimilar structure and polarities may "cancel each other out" thus having a minimal effect of the OD of the mixture under study.
So to PROVE an absolute purity of X, Y, or Z chromatography is the standard.
Jim
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