Witchdoctor
New Member
Super super slow, filtering time is at least twice as long depending on the type of oil used....
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XKawN homebrewing bible
- Thread starter master.on
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TRT
New Member
Same here. Who's selling these filters? I know medlab isn't.
Can't remember off the top of my head. They are out there, I've def seen them. I want to say I've seen them as an off brand like celltreat or something.
master.on
New Member
Is this a serious post?
Most brewers can't afford a five grand autoclave so you come up with six figures worth of equipment? WTF?
These tunnels are designed for mass production.
They sterilize 50,000+ vials a day, that's more than 1 MILLION vials a month!
Do you already sell that much?
I don't even think
-Naps good ol' days
-Med & lab supplies (empty vials)
-perhaps not even Watson T cyp sells a million vials a month
Autoclaving works wonders for small production volumes
Great
But wouldn't be easier and cheaper to buy sterile vials from TOP NOTCH companies?
No homemade Ebay vials
A fuck buddy?
Sorry couldn't resist
I realize alcohol is far from ideal.
But let's face it, most UG gear is poured in unsterile vials
so alcohol may provide a partial solution to lazy, ignorant or greedy UG brewers.
Actually some studies proved that 0.1 um filters retain some bacteria that passes thru 0.22 ones
Method for qualifying microbial removal performance of 0.1 micron rated filters. Part I: characterization of water isolates for potential use as st... - PubMed - NCBI
Method for qualifying microbial removal performance of 0.1 micron rated filters. Part II: preliminary characterization of Hydrogenophaga (formerly ... - PubMed - NCBI
Method for qualifying microbial removal performance of 0.1 micron rated filters. Part III: bacterial challenge tests on 0.2/0.22 and 0.1 micron rat... - PubMed - NCBI
Method for qualifying microbial removal performance of 0.1 micron rated filters. Part IV: Retention of hydrogenophaga pseudoflava (ATCC 700892) and... - PubMed - NCBI
I guess MCT will work fine thru 0.1 um
At the end you can only know by trying
I didn't find any online info for that
EMD Millipore here
Millex-VV Syringe Filter Unit, 0.1 µm, PVDF, 33 mm, gamma sterilized | SLVV033RS
Millex Sigma Aldrich
http://www.sigmaaldrich.com/catalog/product/sigma/f7523?lang=en®ion=US (Millex® syringe filter units, disposable, Durapore® PVDF pore size 0.1 μm, γ-irradiated | Sigma-Aldrich)
Nalgene bottle top (scroll to bottom)
Nalgene™ Rapid-Flow™ Sterile Disposable Filter Units with PES Membrane
Whatman Anotop
http://www.sigmaaldrich.com/catalog/product/aldrich/wha68091112?lang=en®ion=US (Whatman® Anotop® 10 syringe filter sterile, 0.1 μm, 50/pk | Sigma-Aldrich)
http://www.gelifesciences.com/webapp/wcs/stores/servlet/ProductDisplay?categoryId=119766&catalogId=10101&productId=15339&storeId=11787&langId=-1 (GE Healthcare Life Sciences "Anotop 25 mm Sterile Syringe Filters, 0.1 µm (50 pcs)")
Sartorious
master.on
New Member
What do you guys think about these $200 Amazon autoclaves?
They mention surgical equipment so I guess they would be enough for vials
as long as you don't need many of them in a short time
Would rubber stoppers resist autoclaving in these ones?
They mention surgical equipment so I guess they would be enough for vials
as long as you don't need many of them in a short time
Would rubber stoppers resist autoclaving in these ones?
master.on
New Member
What about these SEOH filters?
Syringe Filters Nylon 25mm 0.2um 10pack
Syringe Filters PES 25mm 0.2um 10pack
Consumables - Filters - Syringe Filters - Page 1 - Scientific Equipment of Houston
Nylon or PES?
They are cheap, but are they good enough?
Jeremy: There's no such a thing like cheap and cheerful; there's cheap and nasty and there's expensive and cheerful.
Jeremy: There's a very good reason why its cheap, it's crap!
Jeremy: "yes but there's lots of things that are cheaper than that. I mean, would you like to have, keyhole surgery on your scrotum? that's very cheap. d'you want that?"
Top Gear
Syringe Filters Nylon 25mm 0.2um 10pack
Syringe Filters PES 25mm 0.2um 10pack
Consumables - Filters - Syringe Filters - Page 1 - Scientific Equipment of Houston
Nylon or PES?
They are cheap, but are they good enough?
Jeremy: There's no such a thing like cheap and cheerful; there's cheap and nasty and there's expensive and cheerful.
Jeremy: There's a very good reason why its cheap, it's crap!
Jeremy: "yes but there's lots of things that are cheaper than that. I mean, would you like to have, keyhole surgery on your scrotum? that's very cheap. d'you want that?"
Top Gear
mjf
New Member
How exactly did this turn into what it has become?
I realize witchdoctor's initial comments were not exactly welcomed, but I, for one, would like to hear some of his opinions as they relate to the hime brewer. Of course taking into account that alot of us took to homebrewing as a cheaper route and to also know what goes into our gear with the exception of not really knowing the purity of the raws we buy from these suppliers.
For me, my process isn't sterile from start to finish, seeing as I don't have a dedicated clean room. I'm of the thinking that you keep it clean as possible up until the point you start the filtering process, and at that point everything needs to be sterile, as has been stated in this thread.
Most of us here aren't mass producing. I make 50ml at a time at this point. My first brew, I made 3 100ml batches and with syringe filters, it sucked.
I'd like us to share methods to keep us safe while also being mindful of budget and stop posting "youtube science."
I realize witchdoctor's initial comments were not exactly welcomed, but I, for one, would like to hear some of his opinions as they relate to the hime brewer. Of course taking into account that alot of us took to homebrewing as a cheaper route and to also know what goes into our gear with the exception of not really knowing the purity of the raws we buy from these suppliers.
For me, my process isn't sterile from start to finish, seeing as I don't have a dedicated clean room. I'm of the thinking that you keep it clean as possible up until the point you start the filtering process, and at that point everything needs to be sterile, as has been stated in this thread.
Most of us here aren't mass producing. I make 50ml at a time at this point. My first brew, I made 3 100ml batches and with syringe filters, it sucked.
I'd like us to share methods to keep us safe while also being mindful of budget and stop posting "youtube science."
Sk8man101
Well-known Member
There is bacteria in the air and all around us, most strains are harmless. Even if you super heat the beaker as soon as it cools if it is sitting out for more than a few minutes, airborne bacteria will inevitably land on it. Anyone who has taken a Microbiology class can attest to this.
Sterilizing everything that gear comes in contact with post-filtration is all that should be required.
Disclaimer: I've never homebrewed, but I do know microbiology so take it for what it is.
Sterilizing everything that gear comes in contact with post-filtration is all that should be required.
Disclaimer: I've never homebrewed, but I do know microbiology so take it for what it is.
Did you have a lobotomy performed on you when you were a child? You asked me what pharma companies do not what is cost efficient for a UGL. Jesus Christ, you're so dumb it makes my head hurt.
Ryno1980
New Member
Sir... that was meth... not test... but it was fire
Witchdoctor
New Member
Buy vials that are already sterile? One they are very expensive, 2 how would u explain to the end user when they see a needle mark on the rubber stopper? If it were me I would wonder if my mailman drew out all the good stuff and put in useless oil.....
And I disagree with your autoclave comment, many quality labs who want to follow guidelines similar to what a real lab would look like would buy a unit....Its a one time investment, only thing to buy afterwards is distilled water....And yes rubber stoppers can be used in a autoclave.....
And I disagree with your autoclave comment, many quality labs who want to follow guidelines similar to what a real lab would look like would buy a unit....Its a one time investment, only thing to buy afterwards is distilled water....And yes rubber stoppers can be used in a autoclave.....
That shit wasn't pharma sterile, but it certainly did its job, lol. Why junkies never complain about infections? I used to run heroine through a cigarette filter.
lol I was wondering y I couldn't stop jacking off and robbing people
Shit I would have use that needle after u in my day
TRT
New Member
Can you really do that? Well I guess it's not any better then heating it on a spoon and sucking it up through the fucking que tip cotton.
Yes u can do that Iv done it with speed
TRT
New Member
I don't know why I find that interesting.
TRT
New Member
You couldn't have used a new one? Wait...this out of a random ash tray?
If needed I'd use a puddle of rain to pull up a shot
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