Usmc88
Well-known Member
I think hes saying that its a better protocol to not attempt separating the equipment if you are already going through the hassle of sterilizing any of them you might as well do them all. After all you can't be too safe
MESO-Rx
Anabolic Steroids
XKawN homebrewing bible
- Thread starter master.on
- Start date
to each thier own. It is not a "necessary" step and I brew while the necessary equipment is being sterilized. I do understand the logic if it were that simple, but your ingredients are not sterile and the fact that your beaker is has a open top while your brewing not to mention your filter has an open top as your pouring oil into it. Doesn't take a genius to see that sterilizing your brewing beaker is a waste of time.
Usmc88
Well-known Member
Yes i understand what you're saying and in the instance its sterilizing while you brew i see the logic as well. I hope to build my own diy "clean room" at some point in which I'll definitely be sterilizing everything. I would however as a personal preference recommend sterilizing before first use at least. No telling what you get in the mail. Could be just paranoia but once again better safe than sorry
Witchdoctor
New Member
Yes....If I explain further u will come back with the same exact question...
All labware should be sterilized.
Am I to assume u dont sterilize the bottom beakers since they only contain sterile oils? Sterilize it once and then keep reusing? Now if u say yes then I am wrong with my theory of sterilizing all labwear.....
I sterilize the media bottles and use them once. I sterilize everything past the filtering process which in the only products that "need" to be sterilized.
master.on
New Member
Fuck you and read below
I know it's funny but it is a proven lab method
(3) Sterilizing by Flaming
The flame from a gas burner effectively sterilizes small glass or metal objects, such as inoculating loops, but one must avoid "frying" the yeast by contact with objects heated in a flame. Dip glass spreaders in alcohol and then use the flame to ignite the alcohol. Place metal tools such as loops in the flame until they are red hot. Cool hot tools by touching them to the agar or inside of a sterile glass tube. However, since flames are dangerous this method can and should be avoided whenever possible. We have found that flaming is really only necessary to sterilize inoculating loops and small instruments. We have not been able to demonstrate any value in flaming the openings of tubes, bottles, or flasks in these experiments.
(4) Sterilizing With Alcohol
In many experimental procedures, the most effective way to sterilize objects is with ethanol. Either 95% or 70% will work. The latter is actually more effective, but the former is often more convenient. Of course the alcohol must be allowed to evaporate or be burned off before the object is used in contact with the yeast. Use a flame to burn off the alcohol, a candle is generally less expensive and safer than a gas burner. The alcohol, more than the heat, does the sterilizing, so just "light" the alcohol to minimize heating and speed up the process. Also, wipe the bench with alcohol before starting an experiment to remove mold-laden dust, the most common source of contamination. If your skin is not particularly sensitive, wipe your hands with a small amount of alcohol, too.
Laboratory Methods
read above
and if they're too dumb to burn the house, they shouldn't be brewing anyway.
BTW keep the alcohol bottle far away from the flame.
Nope
I meant to sterilize the bottom bottle
the bottle below the vacuum filter.
Finally some common sense
We all should ask MedLabSupplies to sell some sterilized bottles that fit their vaccum filters
CAUTION: Use only sterile bottles designed for use with vacuum. Always use safety shield during vacuum procedures.
Nalgene™ Rapid-Flow™ Sterile Disposable Bottle Top Filters with PES Membrane
Sterile vacuum filters for screwing directly onto sterile bottles.
Bottle-top filters, Sartolab BT, Sartorius - LLG WWW-Catalog
BOOM!
That's the way to go.
Remember that most brewers won't even filter or use sterile vials at all.
They want you to believe most steroids are made like this:
when they are actually worse than this
no filtration at all!
Now check a real pharma manufacture for comparison
Awwwww, poor masteron's panties got all bunched up *tear*
How about fuck you and read above, you know, read the shit you post before posting. It specifically says "We have not been able to demonstrate any value in flaming the openings of tubes, bottles, or flasks in these experiments."
This is not a pharmaceutical method. Since you don't understand jack shit and bc I don't want anyone to hurt em selves I'll explain it to you. Sterility is a relative term. There is no such thing as absolute sterility, hence it being a relative term, relative to the application being used. The application you copied and pasted is for a fucking biology class project so they only need sterility uo to the point it won't affect the yeast cultures they plan on growing. This is NOT sterile enough for an injectable drug. The same goes for the alcohol method. This is not effective enough for preparing a parenteral drug. Get the fuck out of here with your YouTube videos. You speak as if you were an authority on what really happens but you know nothing. You couldn't even sell Al Gore on global warming if you tried.
What's everyone's thoughts on sterilization through radiation?
Actually Witch Doc is 100% correct. I use to Clean Labs and reaserch facility's. Everything is sterilization even if the products or oil is not but soon will be. When your talking about bacteria and touch points. There might be bacteria ever where. Or even one speck of area. When it comes to sterilization some methods may seem redundant or even repetitive or over kill there is a reason why there are procedures. The world of bacteria is nothing to take lightly when introducing foreign contaminates to the body.
are we comparing pharmaceutical laboratories to homebrewing? If so, then there would be no such thing as homebrewing. Use some logic.
master.on
New Member
Did you mean your gf panties?
The ones she left in my apartment?
Of course flaming alcohol ain't the ideal method you...
The best method is autoclaving
but since most UG gear is made on the (dirt) cheap
alcohol makes a good cheap alternative but of course still inferior to an autoclave
We don't need to sterilize the OPENING of bottles
we need all of them sterile, especially the inside
that's why direct flaming with the burner won't work
because of the narrow neck
It will work
as long as you are able to buy russian uranium without ending up in gitmo LOL
now seriously
define radiation
UV is quite safe but I guess it will fall short if used as the only method
You should clean the table top (kichen cabinet top for most) with alcohol
so cheap, quick and effective
If you really care about cleanliness and sterility
You can make a sterile glovebox
Glovebox - Wikipedia
of an old fish tank or aquarium
or plastic container
DIY Glove Box | Make:
(but still needs additional sealing)
so you can clean the whole thing with alcohol, formaldehyde or some other chemicals
Sterilization (microbiology) - Wikipedia
but don't flame it LOL
standard glass won't stand it
With sterile 0.22 filters sterile vials, a LITTLE ba
you can actually get pharma grade-sterility
without spending six figures on pharma equiment.
Hilarious man. Hilarious and original....
stop pretending as if you know what you're talking about and/or pretending like you're any sort of authority figure on the topic. Autoclaving is ideal only for certain applications. For glass vials and beakers it is NOT the ideal choice of sterilization.
No, you cannot get pharma grade sterility in your house no matter what you do. Period. End of story.
master.on
New Member
Prove me wrong then
What is the proper way for big pharma to sterilize vials, you believe?
Please only quote Good Manufacturing Practice processes used by big pharma.
I am finding companies that are local and will sterilize products
via gamma or E Beam. A properly washed and bagged product that gets one of those treatments would be satisfactory, would it not? I'm in the northeast so maybe this isn't a common industry throughout the USA but I am finding this service up here.
The pricing is typically based on cubic footage and I am finding that no minimum size requirements are needed at most facilities.
If one was to brew for more than personal use this may be a good option if quantities are large enough.
But this is all personal speculation based on some internet digging....
via gamma or E Beam. A properly washed and bagged product that gets one of those treatments would be satisfactory, would it not? I'm in the northeast so maybe this isn't a common industry throughout the USA but I am finding this service up here.
The pricing is typically based on cubic footage and I am finding that no minimum size requirements are needed at most facilities.
If one was to brew for more than personal use this may be a good option if quantities are large enough.
But this is all personal speculation based on some internet digging....
What are you trying to sterilize?
Generally, lab glass wear and vials.
I use my oven after a thorough cleaning, but what I am getting at is there are plenty of professional ways to sterilize that don't include alcohol or any other pea brained methods as one member is peddling as of recent.
The only reason I dug as far as I did was to make sure it was a realistic option and had enough of a foundation for me to quote without being horrifically incorrect, I think I'm not anyway.
Witchdoctor
New Member
One other point I would like to make, everyone is so use to .22 filters, but for almost a year or so there has been .10 filters introduced to the market. IF I were a lab I would be using them as they trap more crap than a .22
The downside is filtering takes longer as the pores are even smaller thus the flow rate is slower.....
The downside is filtering takes longer as the pores are even smaller thus the flow rate is slower.....
I've seen them but everyone talks about .22 as a standard. So the only real inconvenience would be a longer filter time? Small price to pay IMO. Shit, MCT filters painlessly through a .22 filter. How much slower could it really be? I want to get one and try : )
