FastBun
Member
So much cum from all the ridding in this damn thread… I can hardly scroll… where my BETAAAA’s at??? Same shit Millard was talking about in regard to defending sources….
MESO-Rx
Anabolic Steroids
The Lobster International, US and EU Domestic HGH, PEPTIDES and Turkish Pharmacy
- Thread starter TheLobster
- Start date
BamaCrazy
Member
There’s defending sources, then there’s riding their asses like a pony pretending to know their operation better than the source does. Once a source attracts the attention of these two the thread goes to shit. It’s the messenger that’s the problem, not the message.
FastBun
Member
Pony…. Umm not sure…. Looks more like stallion ridding…
Spaceman Spiff
Member
Agreed
Can't let logical thinking go out the window. We aren't defending @TheLobster because we want cheap prices or to suck him off.
Being to extreme on either end is ridiculous. We are discussing getting testing done. My point of it was to prove if degradation was really an issue but now we are getting pushback from the loser who makes it sound like a certainty.
As I said, he could be right but why the hell arent people letting test results speak for themselves?
I
Juiced_lizard
Member
All of this vacuum talk…might as well bring up the room temp, fridge or freezer storage debate?
Already tested and put to rest
I'm in (again). No draft from the Lobster yet.
Release vacuum in lyo hgh vials with air and test at 0, 1, 3, 6, 12 months at x temp? Define.
Also need to agree what we are testing for. Monomer loss? Total oligomers? Monomer percent of total mass by SEC?
See paper earlier. Answer was given for accelerated aging condition at 323 K = 50 deg C = 122 deg F.
Note: 25 deg C is 77 deg F ~ room temp
See also
Might as well get some useful data since the thread "went to shit". Let's see who is down.
Release vacuum in lyo hgh vials with air and test at 0, 1, 3, 6, 12 months at x temp? Define.
Also need to agree what we are testing for. Monomer loss? Total oligomers? Monomer percent of total mass by SEC?
See paper earlier. Answer was given for accelerated aging condition at 323 K = 50 deg C = 122 deg F.
Note: 25 deg C is 77 deg F ~ room temp
See also
Might as well get some useful data since the thread "went to shit". Let's see who is down.
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122 deg F (50 deg C)
See Fig. 4 (again).
Room temp results should be much better. Rule of 10 deg C tells us that chemical reaction rate should be 2x higher with every 10 deg C of increased temp for activation energy of 50 kJ/mol (yes it is a shortcut approximation). So the difference between 25 deg C vs 50 deg may be dramatic.
Fig. 4 shows the importance of excipient package. Kudos to Lobster for having one even if he won't share the exact details including glycine content.
Enjoy the whole paper. So much in here.
Fig 4
Check out those excipients playing their role.
Beautiful kinetic treatment in the discussion section.
Yes the kinetics will slow at 298K vs 323K. Still gives you some great insight of physics/chemistry involved when storing lyophilized GH in air over time.
Now if that ain't some fun bedtime reading I don't know what is.
Protein Quantity on the Air-Solid Interface Determines Degradation Rates of Human Growth Hormone in Lyophilized Samples - PMC
rhGH was lyophilized with various glass-forming stabilizers, employing cycles that incorporated various freezing and annealing procedures to manipulate glass formation kinetics, associated relaxation processes and glass specific surface areas ...
pmc.ncbi.nlm.nih.gov
Fig 4
Check out those excipients playing their role.
Beautiful kinetic treatment in the discussion section.
Yes the kinetics will slow at 298K vs 323K. Still gives you some great insight of physics/chemistry involved when storing lyophilized GH in air over time.
Now if that ain't some fun bedtime reading I don't know what is.
See Fig. 4 (again).
Room temp results should be much better. Rule of 10 deg C tells us that chemical reaction rate should be 2x higher with every 10 deg C of increased temp for activation energy of 50 kJ/mol (yes it is a shortcut approximation). So the difference between 25 deg C vs 50 deg may be dramatic.
Fig. 4 shows the importance of excipient package. Kudos to Lobster for having one even if he won't share the exact details including glycine content.
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Or here's a shortcut method that has limitations:
Tabular format
Sincerely,
not an expert
Accelerated Aging Time Calculator - TAG3
Accelerated Aging Time Calculator TAG3 Tools & Resources Optimize Your Product Lifecycle with Our Accelerated Aging Calculator Understand and predict product durability with our industry-standard accelerated aging calculator, designed to help you streamline testing timelines and ensure...
www.tag3engineering.com
Tabular format
Accelerated Age Calculator
rmtesting.com
Sincerely,
not an expert
Then what is in the non-vacuum vials?
I'm browsing around, trying to decide who do buy ~600 IU from. Not a huge amount, I know, so I don't think I'm some big spender or anything, but just pointing out I'm asking this as a potential customer and not just trolling.
But this comment just doesn't make a lot of sense to me. I can understand why there isn't air inside of a vacuum vial - because, you know, it's a vacuum. But what is there instead of a vacuum if it isn't air? Are you pumping argon gas in there for some reason? If it will maintain a seal, why didn't it get vacuumed?
Source stated the issue was repaired.
Search results for query: Vacuum machine
thinksteroids.com
Pharma uses inert gas backfill. I haven't read here that this source does. Hence if vial doesn't have vacuum then it's air.
Sure, I just don't understand what that post means. What is being claimed to be in the non-vacuumed vials if not air?
TheLobster
Subscriber
My friend, dragging this issue out and exaggerating it this much without a clear result shows that they have a bias against me and are trying to act like know it alls. Their attempt to justify themselves with information they read online and their deliberate effort to prolong this discussion was completely unnecessary.
The HGH batches I produce are always between 2,000 and 5,000 vials. I have repeatedly stated that there is no more non-vacuum HGH, yet they kept pushing the topic on purpose.
Now, for the last time, I want to make this CLEAR AND FINAL:
There is no more non-vacuum HGH. The small amount that existed is gone. Those who received non-vacuum HGH were not harmed I know this for a fact. The test results will be published within two weeks.
Making assumptions and exaggerating the issue based on what they read online without ever holding a Lobster HGH vial or testing one is nothing but a deliberate attack against me.
The greatest difference between ignorance and wisdom is that the wise know what they do not know. Socrates
Your opinion is not as valuable as my knowledge. Because your opinion was formed without knowing anything.Harlan Ellison
That's why I was supporting the member with his questions/concerns. If vial doesn't have vacuum then my conclusion would be headspace of air.
As I have stated I have zero bias against you. If/when I switch to UG HGH I'd like to be a customer.
Yet when a member posts he is concerned with his vial pressure I would hope you would avoid pissing on his back and tell him it's raining.
I wish you the best.
AlexDavis43
Member
This looks like version where you're spending other people's money. That's always fun.
How would you do it if you were paying for it by yourself, today?
Are you aware of any freezer vs fridge testing that I'm not aware of? Been looking for these results for ages...
Deezznutzz
Member
So is there a shipping option if you have a USPS PO box? @TheLobster
Feel free to revise/edit as you see fit. I welcome your comments and any comments.
Feel free to tell us how you'd do it on your dime vs a mix of your money and member money. Btw, I'm not asking you for a dime.
Based on the agreed scope and number of members willing to chip in we should be able to get some data even if it is just monomer loss over a few time points.
Or members can use the shortcut method I provided above to give them some information if they don't want to spend any money (free of charge to them). A small gift to MESO science club.
Awfully quiet in here when the dirty details come up. I find you interesting as a scientist. Spaceman brings up testing: I draft an experimental outline. You offer some snark (not the first time lol).
How about your feedback on the proposal along with whether you are in or out? Get out that red pen Dr.
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TheLobster
Subscriber
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