2,4-DiNitroPhenol [DNP]

Heretic

New Member
Credit to the gods that wrote this... :)

HOW TO NOT FUCK UP DNP:

Since some guys have been playing around and disrespecting DNP and then griping to the forums about the painful results, we need to make this VERY specific and VERY correct so that people won't keep jumping for DNP out of curiosity, or without the willpower they need to operate this respondibly. So here are my experienced guidelines to using it the RIGHT way.

FIRST GUIDLINE: Dosing. Use ONLY 200mg a day for the first four days. I don't care that you don't "feel" anything yet and you wanna bump it up. DNP accumulates in the body, and not "feeling" something means NOTHING. It's there, and it's working (the effect on metabolism begins within two hours of the first dose!). Four days will let you test your tolerance: do you have an allergy? Does it give you a rash? etc.
Only after those four days do you bump it up, by 200mg a day. The average dose is 400-600/day, and more than that gets a little severe. A full gram is the highest dose I've heard anyone use. I've used that much, and it's hell. I like to stay around 600 a day, which is HOT but safe and effective. Take caps even hours apart through the day, ending about 4-5 PM.

SECOND GUIDLINE...How to eat on DNP. This is purely personal experience, because some guys like to carb-deplete *before* using DNP (then eat carbs as usual while on), and other guys like a low-carb approach throughout. Both are fine. Using DNP is the only time that fructose is a desireable cutting carb, because it keeps the liver replentished. That reduces lethargy and spares muscle.
Be aware that eating high-carb foods WILL increase the heat sensation within an hour, and last about 2 hours. That means don't eat carbs before bed unless you want those night sweats to be even WORSE.
Personally, I ate whatever the hell I wanted! IHOP, chinese, fajitas...Yes, I burned hot, but I still lost 1.5 pounds every 2 days. Keep protein HIGH for muscles' sake, and try it yourself.

Foods I suggest including:
Blueberry yogurt. Blueberries are excellent antioxidants, and yogurt cultures help with digestive function, gas, and stool consistency (disgustingly soft stools are common during DNP).
Oregano-based foods. Oregano is perhaps one of the most potent antioxidants around,a nd one spoonful counts as a vegetable serving. See this article
Pineapple - I've found that pineapple helps alleviate those "DNP Blues". The fructose helps, and pineapple enzymes aid in protein digestion.
V8 - one 12-ounce can supplies six servings of veggies, concentrated as an excellent source of antioxidants, lycopene, and recovery of electrolytes.
Oatmeal - high-fiber foods are necessary. You'll find out why around, oh, day 5 or so. Trust me.


THIRD GUIDELINE...Supplements and DNP. I suggest:
ECA - DNP is not a stimulant. To keep energy high and aid in fat loss, use an ECA. Some advisors suggest that regular ephedrine is preferable to norephedrine because of the more direct "hit" of energy.
Prohormones - perfectly fine on DNP. I used 1-AD just to help keep strength and muscle up, and it worked fine. No problems here. You won't GROW muscle on DNP, but it'll help with strength and protection.
Obvious stuff - multivitamin, ZMA, etc.
Biotest PowerDrive - No, I'm not pimping Biotest. But PowerDrive is an excellent pre-workout mixture that actually works. Plus it's low-carb (only 15 calories total), so it won't cause carb-heat in the middle of your workout.

Antioxidants - I'm giving my own personal list, and why I use them:
Alpha Lipoic Acid - aids in fat management and blood sugar, and an excellent antioxidant.
Grape seed extract
Syntrax Radox
Green Tea
Inositol - mood enhancement, antioxidant, and muscle support. 1 gram/3x day
Ellagic acid - protects cell DNA/RNA from damage by free radicals, and may even atack cancerous cells. 400mg/twice a day
Fruit antioxidants - beyond-a-century's powder of high-potency natural fruit anti's. 1 gram, 2-3x day.
Trimethylglyceine - antioxidant, helps move fat and blood lipids into the liver and out of the body. 500mg, 2x day.
Vitamins E and C

Supplements NOT to use:
Any medications that suppress energy. No allergy meds, antidepressants, muscle relaxers, or beta blockers. DNP will have you low as it is; don't worsen your body's energy by taking something that suppresses you further.

DRUGS - Sheesh, you'd think I wouldn't have to mention this, but two idiots in particular (right here on this forum) recently affirmed that some people still just don't get it. NO alcohol (not even "moderate"), NO ecstasy, NO GHB, etc. If you don't have the willpower to forego these habits, DNP is not for you.

Syntrax Swole - a personal discovery. I tried Swole while on DNP...once. Two hours of hell, feeling inside-out.

FOURTH GUIDELINE...working out on DNP. Keep lifting short, 30-40 minutes. DNP works very well, causing your body to use 150% or more the calories per action you'd normally use. That means DON'T try to repeat your usual workouts. Drop to moderate weights, 8-12 reps, not to failure, and with plenty of walking rest between sets. You are NOT going to grow muscle on DNP, so don't use your usual heavy routine. Since DNP can cause light-headedness and heat dizzyness, you have my permission to skip squats in favor of leg presses this time.

Cardio is a controversial one. My advice - do NOT do cardio on high doses of DNP (600mg or more). It's dangerous and counterproductive. Below that amount, some cardio is fine, but keep it to 20 minutes and not at full-gallop. Remember, DNP will drain water from your quickly, causing you to leech out minerals, vitamins, and salts. Don't overdo it.

During exercise, consume at least 1 liter of water per 30 minutes of work, whether you're thirsty or not. DNP is evil in the way it blunts thirst, while at the same time doing the cruel trick of bloating your body with water WHILE dehydrating you from water in your organs. MAKE yourself drink. Always folllow DNP exercise with antioxidants, carbs, and this is a good time to use your multivitamin.

Don't feel embarrassed about poor workouts. Just this morjning I did a workout with a whopping nine sets (wimp!) before calling it quits. Listen to your body, and let it tell you when enough's enough; don't guage workouts by what you *usually* can do otherwise.

Here's my research. This is AMAZING! Not only has not a single test found it to be carcinogenic, but test after tyest after test find that DNP actually ATTACKS cancer cells, and helps anti-cancer medications work better, and helps anti-leukemia medications work without destroying cell DNA, and suppresses tumor growth by 20-50%. The summaries are all right here, friends. Karma me up!

DNP is Ames negative, and does not promote tumors. See for yourself at http://toxnet.nlm.nih.gov/

http://www.epa.gov/ttn/atw/hlthef/dinitrop.html (2,4-Dinitrophenol | Technology Transfer Network Air Toxics Web site | US EPA) reports on health risks. While there have not been human studies, animal studies found no cancers caused by DNP administration. It is considered a toxin because it causes nausea, sweating, and weight loss.

Cyberiron.com reports on halth risks from external exposue. In other words, don?t get it in your eyes, or on your skin if you?re allergic. Pretty elementary stuff.

http://www.ebec2000.com/abstracts/056.htm This animal study documents a 64% increase in metabolism. "These findings confirm that DNP effectively increases metabolic rate..." Duh.

Biosource A PDF file about an antidote to DNP.

http://www.boehringer-ingelheim.es/...glesa/cap13.htm finds that DNP did not activate liver enzymes (MAT) associated with liver damage

"Comparative study of toxicity of 4-nitrophenol and 2,4-dinitrophenol in newborn and young rats." Koizumi M, Yamamoto Y, Ito Y, Takano M, Enami T, Kamata E, Hasegawa R. Division of Risk Assessment, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. This study found that DNP can induce death in overdosed amounts, but that up to that point no toxicity was evident, nor were there any abnormalities in physical development.

"Phenol toxicity and conjugation in human colonic epithelial cells." Pedersen G, Brynskov J, Saermark T. Dept of Medical Gastroenterology, Herlev University Hospital, Copenhagen, Denmark.. This study found that DNP has a toxic effect on cells of the colon, with "toxic" defined in two ways: first, it interfered with metabolism (this we know?it?s the intended effect of DNP users!) and second, it interfered with bowel inflammation (not a health risk. This is caused by osmotic effect, with the worst results being softened stools and gas).

"Mechanisms of bacterial resistance to macrolide antibiotics." Nakajima Y. Division of Microbiology, Hokkaido College of Pharmacy, 7-1 Katsuraoka-cho, Otaru, Hokkaido 047-0264, Japan. This study found that antibiotic-resistant bacteria could be thwarted with DNP. "the extent of the accumulated drug in a resistant cell increases as much as that in a susceptible cell in the presence of an uncoupling agent such as?2,4-dinitrophenol (DNP)."

"Absence of Crabtree effect in human melanoma cells adapted to growth at low pH: reversal by respiratory inhibitors." Burd R, Wachsberger PR, Biaglow JE, Wahl ML, Lee I, Leeper DB. Departments of Radiation Oncology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA. Check this out?DNP actually helps make melanoma tumors easier to attack by increasing ratio of oxygen consumption to lactic acid production, while glycolysis remains the same. "Therefore, tumor acute acidification and oxygenation can be achieved by exposure?"


"New insights in the cellular processing of platinum antitumor compounds, using fluorophore-labeled platinum complexes and digital fluorescence microscopy."
Molenaar C, Teuben JM, Heetebrij RJ, Tanke HJ, Reedijk J. Department of Molecular Cell Biology, Leiden University Medical Centre, The Netherlands. DNP is used as a control in tests of antitumor cells because it does NOT bind to cell DNA, nor promote tumors, yet its staining abilities enable tracking of the uptake of antitumor drugs.

Specific inhibition of breast cancer cells by antisense poly-DNP-oligoribonucleotides and targeted apoptosis." Ru K, Taub ML, Wang JH. Department of Biochemistry, State University of New York, Buffalo 14260-3000, USA Are you ready for this? DNP actually INHIBITS (!!!) breast cancers! Yes, not only does it NOT promote cancers, it?s being recognized as a cancer-fighter/blocker. "Two membrane-permeable and RNase-resistant antisense poly-2'-O-(2,4-dinitrophenyl)-oligoribonucleotides (poly-DNP-RNAs) have been synthesized as inhibitors of human breast cancer?fluorescence assay indicates that the targeted antisense inhibition by poly-DNP-RNAs leads to apoptosis of SK-Br-3 cells but does not affect nontumorigenic MCF-10A cells. The control poly-DNP-RNAs with random or sense nucleotide sequence are completely inactive." Plain English? DNP can be synthesized as an anti-cancer compound, because tests show that it blocks mutagens but does NOT affect non-mutagenic (healthy) cells, and has no RNA effects on them.

"Heat shock protein induction by certain chemical stressors is correlated with their cytotoxicity, lipophilicity and protein-denaturing capacity." Neuhaus-Steinmetz U, Rensing L. Institute of Cell Biology, Biochemistry and Biotechnology, NW II University of Bremen, Germany. The thermic effect of DNP induces protein synthesis (heat shock protein, or HSP, synthesis). In fact, it?s quite GOOD at it: "ASA, DNP and CCCP induced HSP at lower concentrations than substances with a similar lipophilicity?"

"Comparative effects of the metabolic inhibitors 2,4-dinitrophenol and iodoacetate on mouse neuroblastoma cells in vitro." Andres MI, Repetto G, Sanz P, Repetto M.
National Institute of Toxicology, Seville, Spain. In this study, DNP?s observed effect was an increase in metabolism (duh!), while the other toxins compared to it had harmful in vitro effects but no increase in metabolism.

"Inhibition of uncoupled respiration in tumor cells. A possible role of mitochondrial Ca2+ efflux." Gabai VL.Medical Radiology Research Center, Russian Academy of Medical Sciences, Obninsk. DNP not only does not cause tumors, but it inhibited their respiration by 20-25% compared to controls.

"Amsacrine-induced lesions in DNA and their modulation by novobiocin and 2,4-dinitrophenol." Shibuya ML, Buddenbaum WE, Don AL, Utsumi H, Suciu D, Kosaka T, Elkind MM. Department of Radiology and Radiation Biology, Colorado State University, Fort Collins 80523. In this study, researchers found that DNP abrogates?or disrupts?cytotoxicity in hamsters (using cancerous cells). They expected to find that DNP would interfere with anticancer treatments, but instead found that DNP increased their effects. They state, though, that they cannot claim a proven effect of DNP on anticancer treatments yet, although they do agree that treatment with DNP actually enhanced the effects of the DNA regenerative therapy of anticancer chemotherapy.

"Induction of endonucleolytic DNA cleavage in human acute myelogenous leukemia cells by etoposide, camptothecin, and other cytotoxic anticancer drugs: a cautionary note." Kaufmann SH. Oncology Center, Johns Hopkins Hospital, Baltimore, Maryland 21205. The authors warn that certain anti-leukemia drugs resulted in "extensive DNA degradation." BUT (good ol? DNP to the rescue!), "Preincubation with dinitrophenol abolished the effect?"

"[Dependence of the nature of the action of metabolic inhibitors on ribosomal RNA synthesis in Ehrlich ascites carcinoma cells on cell integrity]" [Article in Russian] Akhlynina TV, Buzhurina IM, Panov MA, Rozovskaia IA, Chernaia NG. DNP actually inhibits the synthesis of RNA in carcinoma cells. In other words, it helps cancerous cells commit suicide by neutering themselves. "Ribosomal RNA (rRNA) synthesis in the intact Ehrlich ascite carcinoma cells is selectively inhibited by papaverin (ED50 = 0.01 mM), 2,4-dinitrophenol (DPN; ED50 = 5 microM), and actinomycin D (ED50 = 0.1 microgram/ml)."

"Autocatabolism of surface macromolecules shed by human melanoma cells." Bystryn JC, Perlstein J. Cancer Res 1982 Jun;42(6):2232-7. This study finds that DNP helps melanoma cells die (autocatabolize) while other cells are unaffected.

http://www.geocities.com/byggdegstor/dnpforside - tons of research, including medical studies. Excerpts:

DNP does not cause liver damage: "Their analyses demonstrate, beyond a doubt, that the liver does not suffer any damage in the course of dinitro treatment." (Biological Study of Dinitro Drugs in Humans By Dr. Jacques Bell. Bell, Jacques. 1939. Etude biologique des produits dinitres chez l'homme. Medecine. 19:749-54. Translation ? 1996 Robert Ames)

Also: "Experimental studies on animals do not show toxic effects of dinitrophenol on the kidney. Anatomical-pathological examinations of animals, even those which died from a massive dose of dinitrophenol, do not reveal any important anatomical changes, except a small degree of cytolysis. Clinical documents are not abundant, but, on the whole, do not seem to demonstrate that dinitrophenol is toxic for the kidneys."

"Dinitrophenol has almost no action on the blood cholesterol. (Grant and Schube)."

"it doesn't seem that dinitrophenol at usual clinical doses is likely to harm the kidneys."

"Dinitrophenol is remarkable for its absence of effect on the cardio-vascular system...dinitrophenol is absolutely devoid of toxicity for the heart."

"Dinitrophenol does not attack cell tissue albumin and does not determine the fat loss to the expense of the muscles, contrary to thyroxine."

"dinitrophenol offers this precious advantage that the cessation of its use at the slightest appearance of signs indicating an imminence of intoxication results immediately in the arrest of those symptoms." (Professor Pouchet)."


Interestingly, one medical theory on a health ADVANTAGE of DNP is that the slight increase in thermogenic temperature simulates the fever a body induces during a viral attack. The body increases itsheat to protect organs but kill viruses, and some theorize that DNP can do the same thing, thus killing viruses in the body. In this mechanism, DNP may have an immune-enhancing effect.
 
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ziggy said:
Hey bro I appreciate your evidence for DNP however I was a little concerned by the spec sheet for the antibody for DNP as an antidote. Antibodies aren't antidotes; they do however have an integral part in the adaptive immune response. This is done by immature B-cells in the bone marrow being selected for affinity for an antigen. Once selection has taken place the B-cell becomes a plasma cell secreting antibodies specific for the antigen. The way the antibody can kill/remove the antigen is to be recognized by a T-helper cell which is MHC-restricted. Then programmed death (apotosis) can occur. The antibody on the spec sheet is made from a host other than human which means that if you introduced this Ig into a human the host will recognize this as foreign and build an immune response to it. Also if the foreign Ig is able to bind DNP in the host it would not allow program cell death because the T-helper cell has to be MHC restricted meaning that it will not recognize anitigen that is in the binding pocket of the major histocompatibility complex. That's all I have to say about that but I would like to try some DNP, maybe pm me so that I can get some, Thanks


Wow....someone likes to use their science book to write. ;) I'll try and keep my explanation down a little closer to those of us that read plain old English.

Why does DNP work, it basically causes the KATP gateway to stay open, allowing a inefficiency in the formation of ATP (energy packets) and the need for the body to use more fat to produce the same amount of energy. The Antidote (ne: antibody), is actually a proteolytic (sorry...big word) that allows the KATP gateway to be shut....thereby causing DNP to have less effect.

I like your thoughts Ziggy, but in this case the agent acts a little different than through the binding of antibody to the DNP molecule. Cellular death in this case does not result from a suppression of the immune system (re: T-Cell activity). The antibody is not designed to help the immune system, so therefore there is no need for it to be MHC restricted, and at the required doseage and time frame the half-life is such that the immune system should not immediately respond in a fashion which would reduce it's effectiveness.

Bottom line: Keep with the required doses and the last two posts become totally irrelevant unless you are an ER doc who is about to $h!t his pants because he has an idiot laying on a table in a pool of sweat.

As far as "giving it a try", please don't until you read much more research about the drug and the action that it has on a cellular level.

Hope this helps,

Kemo
 
Damn.. now I got questions..

Now after that last post I have questions.... Is it safe or not? Can see the safe dose recommendations and feel that only an idiot would test the limits of those.

Kemo, what other research would you recommend.

I am 38, around 200lbs and play competitive racquetball. I need to cut about 15 pounds. Racquetball has aerobic and anaerobic components. I want to drop some excess weight and then get stronger. Any thoughts?

Talon
 
As far as DNP being safe or not, it's more or less like asking if steroids, insulin, or diuretics are safe or not. Yes and no. If you use them responsibly and follow the rules, you'll be fine. If you fuck around and do stupid shit like use rec drugs or take too much, then you may have a problem. It's clearly explained at the beginning of the post how much to use and for how long, but to recap you take 200mg the first few days to see how you react, and then you can bump it up to 400mg for about a week at most. After that the fat-loss effect becomes greatly diminished, but you can cycle it like gear (time on=time off) until you get where you want to go.
Obviously I have a vested interest in saying it's safer than most people think, but I firmly believe insulin and diuretics are FAR more dangerous than DNP, and look at how many people use those. Look at how many pros have DIED from both of those items, but they don't have the same fear factor that DNP seems too. A healthy respect for DNP is good though, because the only time a drug becomes dangerous is when you lose your fear of it and become careless.
Regards,
-H-
 
heretic-what are your thoughts on a week on, week off cycling of dnp? or would 2 weeks on, 2 weeks off be more effective? running a 4day warm up of 200, then 2 days 400, then 600 to see how my tolerance is at that doseage. then if i take a week off would i have to run the same steps? or can i start right at 400? I will most likely use 400 for a while if its effective enough.

Anyone feel free to leave their opinion on this cycle. thank you.
 
One week would be better than two, like I said after about 9 days it kind of loses it's kick, plus you'll be miserable and want to stop anyway. I'd then take at least a week off, maybe two, to let the water bloat drain out and allow for glycogen supercompensation (anabolic rebound). But damn, when that water comes off and you see the results it's something else. :D
Regards,
-H-
 
PL posted: "but another famous quote is, 'DNP will raise your body temp high enough to kill you!' This also proves that he has never done it because as you will find, your body temp only goes up about one degree. Ok, enough about the fake guru."

DNP will kill a person if doses are too high, to say differently is not only false but also dangerous to people that read that information. This isnt specifically against PL, but anyone who says that DNP cant be lethal. Whether death occurs from a rise in oral temperature or through some other factor isnt important. Id have to look up and see what the reasoning is.

Overall, very good info in this thread.
 
blury, i wouldnt even drink once per week if u r considering the dnp route. dehydration i believe is a major factor in dnp's ability to kill. never done it so stick with info from guys who have
 
The point of that paragraph wasn't that DNP isn't lethal, it was that DNP does not raise core temperature at normally used doses. Your body will radiate heat like a mofo, but if you actually check your temp it's often times LOWER than 98.6 degrees (normal). It's only if your core temperature rises that you may be in danger of death through overheating. Dehydration would be a more likely reason. It has been argued that the guy from EF was killed not by DNP but by the diuretics he was given by the doctors in order to try and flush the DNP out of his system. Obviously this would dehydrate the body and probably fuck up your potassium levels to the point that it could kill you.
Never mind the supposed 155 deaths from Ephedrine.....or the number of people that have died from heart attacks while doing cardio. Again, no one is trying to paint the picture that DNP is no big deal or harmless, just use common sense with it.
 
i am not looking to use it any time soon, im still doing more research on it yet. I noticed the warning about not drinking alchol with it when i read over this, i would deffinitly not be drinking at all while on it. Thanks for lookin out for me bros.
 
I think part of the reason for lower temperature readings is from a depressed thyroid.

Personally, Ill never go above 400mg again and do get results from 200mg. 600mg was way too much for me and I cant even imagine 800+mg.

If you choose to use, just be very careful!
 
using

I started a DNP cycle last night at 400mg/day + T3 at 200mcg/day. I'll keep you guys posted on how i get on. Cant feel much yet, slightly warmer than usual.

Ive used DNP twice in the past and always had great results.
 
crewboss said:
T3 @ 200mcg's / day as a starting dose? am i missing something.


Yeah I thought I was seeing things when I read this also. Hopefully he made amistake and wrote the wrong dosage. From my understanding isnt T3 suppose to be started at 12mcg's/25mcg's ed and worked up slowly from there.
 
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