Plate Smacker
Well-known Member
Recipes
Deca has odd mL because I adjusted mL and overdose to populate/match powder quantity I had.
Deca has odd mL because I adjusted mL and overdose to populate/match powder quantity I had.
MESO-Rx
Anabolic Steroids
BrewPorn
- Thread starter Leancuisine
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Plate Smacker
Well-known Member
Test E left, Deca right.
Going to sealed sterile vials.
FILTER USED
Going to sealed sterile vials.
FILTER USED
Last edited:
Plate Smacker
Well-known Member
Question:
I have 3 GL45 Septa caps, just need septa disks.
Would I get Silicone or PTFE faced?
I have 3 GL45 Septa caps, just need septa disks.
Would I get Silicone or PTFE faced?
Interesting, those are like the zapcaps, not marked sterile
What is the funnel for? I was under the impression that all you need is a bottle top filter and media bottle.
The funnel is the bowl portion of the bottle top filter
Oh okay, I see thanks.
MisterSuperGod
Well-known Member
Now i'm confused. You would pour the unfiltered oil into the filter bowl thing there and let gravity do the rest?
When it's done filtering, then what? Do you just transfer it into vials from there? How do you maintain sterility doing that?
He’s using a hand pump but you could also use an electric vacuum pump which sucks it through the filter. There is a fitting on the filter to attach a line. Yes, you just transfer to vials. Some people put a septa cap on the media bottle. It’s not 100% sterile to do it that way. Only way to be 100% sterile is a true clean room. Bet you worry about your ugl gear now lol
After filtering into a sterile media jar, I have a septa cap, on that jar, that has a hole in it large enough to stick these sterile pipettes through and draw up the oil. Then dispense it into sterile vials and place the stopper on that vial then on to the next. Below is the pipette pump and pipettes I use.
Plate Smacker
Well-known Member
Given an area that has been wiped down, and an air purifier in the room running for some time.
You can simply remove plunger from a 60ml syringe, and backfill by pouring from media bottle.
Dangerous?
Think about filling vials.
You have to uncover the vial to use a pipette to fill. For a short time, exposed.
Have you seen an actual factory setting for vials being filled?
The vials ride along a conveyor, uncapped, then under a couple of fill nozzles. I have seen fill nozzles a half inch above top of vial. Once again, exposed to surrounding air as hormone oil is emitted into vial.
Sure, the area was put together in a precleaned decontaminated room. You have to enter through the decontamination air burst door thingy. I sure don't have that in my bathroom. I also don't wear a suit like I am about to make contact with alien life forms.
Me pouring filtered gear from a sterilized media bottle, half inches from a sterile 60ml syringe topped with a 20G needle, then placing plunger back into opening, flipping over then seating plunger.... into a vented sealed vial... works just fine.
I have not had any issues.
I do have GL45 open top caps, and awaiting septas. Turns the media bottle into a large vial.
That is my method in the future.
However, for the longest, I used method outlined above.
You can simply remove plunger from a 60ml syringe, and backfill by pouring from media bottle.
Dangerous?
Think about filling vials.
You have to uncover the vial to use a pipette to fill. For a short time, exposed.
Have you seen an actual factory setting for vials being filled?
The vials ride along a conveyor, uncapped, then under a couple of fill nozzles. I have seen fill nozzles a half inch above top of vial. Once again, exposed to surrounding air as hormone oil is emitted into vial.
Sure, the area was put together in a precleaned decontaminated room. You have to enter through the decontamination air burst door thingy. I sure don't have that in my bathroom. I also don't wear a suit like I am about to make contact with alien life forms.
Me pouring filtered gear from a sterilized media bottle, half inches from a sterile 60ml syringe topped with a 20G needle, then placing plunger back into opening, flipping over then seating plunger.... into a vented sealed vial... works just fine.
I have not had any issues.
I do have GL45 open top caps, and awaiting septas. Turns the media bottle into a large vial.
That is my method in the future.
However, for the longest, I used method outlined above.
The glass pipette goes inside the plastic pump?
One end of the pipette sticks into the pump then you roll the wheel and the plunger goes up and creates a vacuum that pulls the liquid into the sterile pipette
Wow very interesting! Thanks for that, I'll pick them up if I ever start capping.
I pour into a 60ml now. Hell, air balances in/out of vials every time you draw (hence BA in solution). I'm not saying leave the caps off sitting there and AC unit blasting but this works and is solid. Drawing from a media bottle sucks but I like septas for when I'm in a hurry and lazy.
I put small squares of tinfoil on the washed vials and then bake them in the oven to sterilize. I pull off the foil right before I need to insert pipette then I have the sterilized stoppers available and put one on the vial once it is filled. Then I crimp the caps after they are all filled and covered by the stopper.
Another advantage of the small foil square is that the vials will remain clean if they aren’t used and I just re-bake them when I need them the next time.
Fancy
Plate Smacker
Well-known Member
I will be using the GL45 open cap with septa on the media bottle. Example: Test E will be stored in the media bottle, and when I need a new vial I will be using 12ml sterile syringe with 20G needle, to draw 10 ml and transfer to vented sealed sterile vial. Takes less than 3 minutes. Once that vial is used up, I toss the vial, and use a new one. I have reused the vial once in the past, because I was waiting on new vials to come in that I ordered last second.
Now I just keep a stock of no less than 10 empty vials.
I did end up filling more vials for each compound I will run through the year, so I am set.
Anyone else use these? Thinking about grabbing some
