Elite Labs

[wedorecover]

Dibs on ass.

We will make her air tight I wouldn't want air to get in there and make it unsterile

 

[CdnGuy]

Aseptic technique, such as what we practice when we inject, is very prudent, of course, but I have a few closing remarks. if the autoclave is unnecessary, and heating the final solution is apparently unnecessary, what is required for a safe injectable product? Well, it's stupidly simple. filtering IS "sterilization" for all intents and purposes. but if there's nothing to filter out, then you can in fact just mix powder and oil and inject. If there's no contaminant there's no contaminant, but filtering the final product is really the only precaution you need to take for mass distribution. I have a mason jar that I bleached and dried and then mixed tren in. I pull out an ml at a time, attach my syringe filter, and filter the product directly into another syringe, and then attach the needle and inject. Never had one injection complication in 1.5 years. Final filtration with a .22 micron filter I believe is the pharmaceutical standard for sterility. That's all a UGL needs, is filters, in spite of all your bullshit for "meso standards." lmfao

So don't say I didn't tell you so, but I did. All you fuck sticks scrutinizing this guy's brewing methods and apparatus don't know what the fuck you're talking about. You're a fucking joke meso with maybe 2 members worth their salt.

 

[colossus25]

View attachment 68594

Lmaoo

 

[cdt5074]

@cdt5074 please explain more about filtering directly into a new syringe. Are you pulling the plunger all the way out and filling from the large end or injecting through the small end after pulling the plunger to the required (1cc) doseage?

ask colossuscuntfucker69 he knows everything apparently. except the difference between aseptic and sterile technique but here at meso he gets to be retarded and gets validation for it. he also doesn't understand how using syringe filters works. hey colossuscuntfucker69 let's learn math. if I suck 1 dick everyday for 7 days, I sucked 7 dicks. if I suck 7 dicks at once, I also sucked 7 dicks. does 7=7?

HAHAHAHAHAHAHAHAHAHAHAHAHAHHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAAHHAHAHAHAHAHAHAHAAHAHAHAHAAHAHAHAHAHAHAAHAHAHAHAHAHAHAHAHAHAAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAAHAHHAHAHAHAHAHAHAHAHAHAHAHAH

I'm glad he fits in somewhere. he really does try oh so hard. but my momma raised me not to make fun of retarded folk so I'll let him alone from now on.

 

[cdt5074]

We will make her air tight I wouldn't want air to get in there and make it unsterile

what...oh you're an idiot and you're making a joke at your own expense? you guys are good sports about it at least. let me try:

yeah and you should wear a condom too because you wouldn't want to get syphilis or another STD, as if condoms statistically protect against that sort of thing am I right boys? eh? LOL.

Being stupid if fun!

 

[Johne71]

Not many people (unless they work in the industry know the differenc between aseptic and sterile). That's been covered. I still want to learn about your method of transferring from one syringe to another. You claimed to do this and I'm curious how. It's not a crazy idea I'm just not understanding how to make it work without it being very cumbersome.

 

[cdt5074]

Not many people (unless they work in the industry know the differenc between aseptic and sterile). That's been covered. I still want to learn about your method of transferring from one syringe to another. You claimed to do this and I'm curious how. It's not a crazy idea I'm just not understanding how to make it work without it being very cumbersome.

No he's a moron. I mean you read his posts. I would lose a lot of respect for you if you defended that guy. He's stuck out of time stupid.

It's incredibly efficient, actually. I do not have a vacuum filtration system and do not use caulk guns. What you do is take a 3 ml syringe and draw up the desired amount of oil. You attach a syringe filter and needle. You get a second syringe and pull back the plunger all the way. You dispense the filtered oil through the neck of the second syringe and then attach a needle and inject. This is literally the most efficient way to home brew, as it does not require anything besides a syringe filter to make and sterilize your gear. And if you understand physics at all, you would understand that filtering with 3ml syringes makes the Filtering process effortless. The resistance through the filter is negligible compared to a 60ml syringe, which requires a caulk gun and is an easy way to burst a filter.

I know my shit. Glad to share knowledge with someone who appreciates it.

 

[Johne71]

Much appreciated and quite interesting. Unique in my eyes and have never heard it done that way. At first I thought you were completely pulling out the plunger and filling. I've tried this with different substances before where I have worked and thought it was a major pain in the butt to get the plunger back in under pressure of the filter. Nice idea and thank you for sharing.

I'm not here to defend, refute, argue, gain respect.etc. There is quite plenty of that to offer and I'm not here to sing praises or make enemies. Life is too short and I am who I am. On the forum and in person. Same dude here.

The information is appreciated and thank you for the explanation.

 

[jaymaximus]

I know my shit. Glad to share knowledge with someone who appreciates it

Hey man. I've been appreciating you all day. I think its bullshit you don't appreciate my appreciation. Some new dude shows up and you appreciate his appreciation while not appreciating the appreciation that I've been appreciating upon you.

 

[colossus25]

ask colossuscuntfucker69 he knows everything apparently. except the difference between aseptic and sterile technique but here at meso he gets to be retarded and gets validation for it. he also doesn't understand how using syringe filters works. hey colossuscuntfucker69 let's learn math. if I suck 1 dick everyday for 7 days, I sucked 7 dicks. if I suck 7 dicks at once, I also sucked 7 dicks. does 7=7?

HAHAHAHAHAHAHAHAHAHAHAHAHAHHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAAHHAHAHAHAHAHAHAHAAHAHAHAHAAHAHAHAHAHAHAAHAHAHAHAHAHAHAHAHAHAAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAAHAHHAHAHAHAHAHAHAHAHAHAHAHAH

I'm glad he fits in somewhere. he really does try oh so hard. but my momma raised me not to make fun of retarded folk so I'll let him alone from now on.

Hey id rather be a cunt fucker than a dick sucker like you champ lmaoo

 

[colossus25]

No he's a moron. I mean you read his posts. I would lose a lot of respect for you if you defended that guy. He's stuck out of time stupid.

It's incredibly efficient, actually. I do not have a vacuum filtration system and do not use caulk guns. What you do is take a 3 ml syringe and draw up the desired amount of oil. You attach a syringe filter and needle. You get a second syringe and pull back the plunger all the way. You dispense the filtered oil through the neck of the second syringe and then attach a needle and inject. This is literally the most efficient way to home brew, as it does not require anything besides a syringe filter to make and sterilize your gear. And if you understand physics at all, you would understand that filtering with 3ml syringes makes the Filtering process effortless. The resistance through the filter is negligible compared to a 60ml syringe, which requires a caulk gun and is an easy way to burst a filter.

I know my shit. Glad to share knowledge with someone who appreciates it.

Bro this is the stupidest thing i think I've ever read in my life. I don't even have to say anything further, you're making a fool out of yourself on your own. Keep it coming bro i wanna know more about all the "shit" you know [emoji23][emoji23][emoji23][emoji23]

Is this real life right now.......

 

[colossus25]

No he's a moron. I mean you read his posts. I would lose a lot of respect for you if you defended that guy. He's stuck out of time stupid.

It's incredibly efficient, actually. I do not have a vacuum filtration system and do not use caulk guns. What you do is take a 3 ml syringe and draw up the desired amount of oil. You attach a syringe filter and needle. You get a second syringe and pull back the plunger all the way. You dispense the filtered oil through the neck of the second syringe and then attach a needle and inject. This is literally the most efficient way to home brew, as it does not require anything besides a syringe filter to make and sterilize your gear. And if you understand physics at all, you would understand that filtering with 3ml syringes makes the Filtering process effortless. The resistance through the filter is negligible compared to a 60ml syringe, which requires a caulk gun and is an easy way to burst a filter.

I know my shit. Glad to share knowledge with someone who appreciates it.

@Johne71 don't listen to that ass clown. All he uses is a filter right? Yeah well here is a study which proves that there are bacteria that can pass through a .22 filter. This is why we practice aseptic techniques.

Filterability of staphylococcal species through membrane filters following application of stressors

 

[cdt5074]

Bro this is the stupidest thing i think I've ever read in my life. I don't even have to say anything further, you're making a fool out of yourself on your own. Keep it coming bro i wanna know more about all the "shit" you know

[emoji23

Bro this is the stupidest thing i think I've ever read in my life. I don't even have to say anything further, you're making a fool out of yourself on your own.

Hey what's 1+1? And you can't look it up or use a calculator! No cheating! You silly goose.

 

[insertnamehere]

Hey man. I've been appreciating you all day. I think its bullshit you don't appreciate my appreciation. Some new dude shows up and you appreciate his appreciation while not appreciating the appreciation that I've been appreciating upon you.

Jaymax, I appreciate the appreciation you've been appreciating to mister needing appreciation all day, bro

 

[cdt5074]

@Johne71 don't listen to that ass clown. All he uses is a filter right? Yeah well here is a study which proves that there are bacteria that can pass through a .22 filter. This is why we practice aseptic techniques.

Filterability of staphylococcal species through membrane filters following application of stressors

Hey you leaned a New word today and used it in a sentence! I'm so proud of you boo!

 

[colossus25]

Hey you leaned a New word today and used it in a sentence! I'm so proud of you boo!

Hey you learned that there are species of bacteria that can pass through filters and you're still talking shit. You go girl!!!

 

[colossus25]

This guys only defense is that i used the wrong terminology when describing the techniques every brewer worth his salt uses to ensure a quality and safe product. But hey @jaymaximus is right we should appreciate him for sharing his syringe to syringe technique that is about as safe as a the nuclear football in trumps hands. WE APPRECIATE YOU BROOOO!!!

 

[Johne71]

They do make smaller pore size syringe filters as well (0.1micron). The concentration of cells that could be in your oil is probably very low, especially if it is also filter sterilized, so I would feel pretty confident in using the 0.22. It's all we ever used in the lab I worked at. In the study, they are filtering and checking for growth on a Petri plate with a nutrient agar which is a favorable growth media for those cultures.

From reading the home brew section I thought many people were only heating to assist getting the powder into solution. Some hormones (Test E) I thought some weren't heating at all.

I have a lot of reading to do and purchases to make before going there.

 

[Docd187123]

Because it has nothing to do with the discussion. Those companies have to comply with federal regulations. Why do those federal regulations exists? Because they do?

It has everything to do with the discussion but if you're too simple minded to understand the connection, what would you like me to do? Dumb it down even more so you can grasp it?

Hmmmm, ok, so the FDA regulates pharmaceutical companies and makes them sterilize empty vials in aseptic processing. You're answer as to why the FDA mandates this is bc they do. Besides that being circular reasoning and an incredibly easy question to answer, you still can't answer it yet you're the sterility guru here to impart unfathomable wisdom on the forum lol?? I promise you it's not a trick question and the answer is simple enough.

What kind of question is that? You are trying to trip me up by asking erroneous questions.

If that simple question trips you up then it's like Sammy Sosa being struck out by a 12yo pitching underhand. You say it's erroneous only bc you fail to see the implications and line of reasoning.

I don't know anything about the process for determining sterilization protocols for pharmaceutical companies,

Don't worry, it's abundantly clear you don't know anything and I'd never think you do.

and it has nothing to do with this discussion.

Of course it does. If you can't see why then you really have no business in this discussion.

We're talking about sterilization methods for UGL's, if you weren't aware.

If you could choose between a bottle of Watson test cyp and some random UGL's test cyp and both are the same price, which you choose? My money is on you choosing the Watson bc you're assured strict quality, consistency, accuracy, and sterility. So the best way for a ugl to ensure its products are consistent, have accurate dosing, are sterile, etc is to try and mimick pharmaceutical procedures as closely as possible.

 

[Docd187123]

I think we are confusing sterile vs aspectic technique. There is a pretty big difference. I'd just say we can end this argument by stating there is no UGL using sterile techniques. I hope this is what @cdt5074 is getting at. In the field of production etc, there is a huge difference between the two. Mostly for brewing AAS we are trying to achieve aseptic conditions. I'm impressed if anyone has a cleanroom in their place of residence. Without sharing too much, I come from 20+ years dealing directly with industrial microbiology and pharmaceuticals. Problem with autoclaving...that glass needs to be clean before autoclaving. If that glass is full of debris...still going to be full of sterile debris when autoclaved. Better off lightly covering with foil and baking after cleaned. Then using aseptic techniques to fill the vials and replace the tops aseptically as possible...which is what @colossus25 has described.

What are your thoughts on the following:

According to the FDA and USP, all parenteral drugs and their containers must undergo treatment to make them both sterile and non-pyrogenic. Terminal sterilization is not an option for AAS bc they are thermolabile, hence the need for aseptic processing.

For the oil, a basic 0.22um filter will make the AAS solution sterile but not necessarily non-pyrogenic bc size of endotoxins can be smaller than a 0.22um membrane. Since endotoxins are negatively charged at a Ph >2, the use of a positively charged 0.22um filter can trap more endotoxins than a non-charged filter can. This will make the solution both sterile and pyrogenic.

For glassware, including vials, the same requirements are necessitated but you can't filter a vial lol. Even with a suitable wash beforehand, autoclaving will only sterilize the glassware but not make it pyrogen free. The FDA and USP define something as non-pyrogenic when there is a log 3 reduction in pyrogens when starting with a minimum concentration of 1000 EU (endotoxin units) or 99.9% inactivation/destruction. Autoclaving cannot accomplish this due to the heat resiliency of endotoxins and pyrogens. A much better option is dry heat 250 deg C for a minimum of 30min. This process has been validated at achieving a log 5 reduction in endotoxins or 99.999% kill rate. Depyrogenation with dry heat acts linearly up until log 3 reduction and biphasically afterwards so you'll need much more time for a higher degree of depyrogenation.

For stoppers since they are not as heat resistant as glass, the best way is through autoclaving. It's beleived autoclaving in the presence of H2O2 is more effective at depyrogenation that autoclaving with just water.