Elite Labs

[colossus25]

It has everything to do with the discussion but if you're too simple minded to understand the connection, what would you like me to do? Dumb it down even more so you can grasp it?

Hmmmm, ok, so the FDA regulates pharmaceutical companies and makes them sterilize empty vials in aseptic processing. You're answer as to why the FDA mandates this is bc they do. Besides that being circular reasoning and an incredibly easy question to answer, you still can't answer it yet you're the sterility guru here to impart unfathomable wisdom on the forum lol?? I promise you it's not a trick question and the answer is simple enough.



If that simple question trips you up then it's like Sammy Sosa being struck out by a 12yo pitching underhand. You say it's erroneous only bc you fail to see the implications and line of reasoning.



Don't worry, it's abundantly clear you don't know anything and I'd never think you do.



Of course it does. If you can't see why then you really have no business in this discussion.



If you could choose between a bottle of Watson test cyp and some random UGL's test cyp and both are the same price, which you choose? My money is on you choosing the Watson bc you're assured strict quality, consistency, accuracy, and sterility. So the best way for a ugl to ensure its products are consistent, have accurate dosing, are sterile, etc is to try and mimick pharmaceutical procedures as closely as possible.

Knowledge is a dish best served cold. I always appreciate your knowledge bombs doc.

 

[cdt5074]

This guys only defense is that i used the wrong terminology when describing the techniques every brewer worth his salt uses to ensure a quality and safe product. But hey @jaymaximus is right we should appreciate him for sharing his syringe to syringe technique that is about as safe as a the nuclear football in trumps hands. WE APPRECIATE YOU BROOOO!!!

Yeah? Syringes are sold sterilized. Transferring from a sterile syringe into another sterile syringe is And let me check....safe! It meets your aseptic or sterile? Standards? Which was it again? I get those two confused all the damn time! Help me out I heard you were the expert!

 

[colossus25]

Yeah? Syringes are sold sterilized. Transferring from a sterile syringe into another sterile syringe is And let me check....safe! It meets your aseptic or sterile? Standards? Which was it again? I get those two confused all the damn time! Help me out I heard you were the expert!

First off i never claimed to be an expert at anything so fail there champ. Second i posted a research study which shows that there are bacteria that can pass through a .22 membrane. You do you man i just personally rather be safe than sorry and follow proper lab procedures instead of doing some sketchy shit.

 

[cdt5074]

Hey you learned that there are species of bacteria that can pass through filters and you're still talking shit. You go girl!!!

Do I have to to do this to you again? Really? Do you get off on it or something? remember .22 filtering is a pharmacy standard for a fucking reason.


Staphylococcus is something that is generally harbored on human Skin. How the fuck it would get in your gear I have no idea. You would have to wipe your ass and stick your hand in your fucking gear, which you might do. So I will continue. Staph is an aerobic Organism, and it cannot survive or grow in oil. So even if you got staph in your vial, how do I say this politely...it would make no fucking difference. It's not gonna make it, just like your dignity from this fucking thread. And let's just say you have staph in your vial or whatever the fuck your gear is in: heat sterilization won't kill the endotoxins it produces even if it kills the staph.

You get staph from...wait...was it aseptic or sterile injection technique. Fuck I forget. You know which one it is right? Help a brother out.

 

[colossus25]

Do I have to to do this to you again? Really? Do you get off on it or something? remember .22 filtering is a pharmacy standard for a fucking reason.


Staphylococcus is something that is generally harbored on human Skin. How the fuck it would get in your gear I have no idea. You would have to wipe your ass and stick your hand in your fucking gear, which you might do. So I will continue. Staph is an aerobic Organism, and it cannot survive or grow in oil. So even if you got staph in your vial, how do I say this politely...it would make no fucking difference. It's not gonna make it, just like your dignity from this fucking thread. And let's just say you have staph in your vial or whatever the fuck your gear is in: heat sterilization won't kill the endotoxins it produces even if it kills the staph.

You get staph from...wait...was it aseptic or sterile injection technique. Fuck I forget. You know which one it is right? Help a brother out.

Bro what is your deal lmaoo this guy is such a troll im done with you, you already proven your complete incompetence for lab procedures and lack of knowledge. Lol have a nice life champ.

 

[colossus25]

Unlike you and your wacky ass procedure i don't have to filter every shot. My gear stays completely aseptic, all i have to do is draw and pin. If you want to filter every shot because you dont want to go through the process of makimg your vials aseptic thats on you man.

 

[cdt5074]

What are your thoughts on the following:

According to the FDA and USP, all parenteral drugs and their containers must undergo treatment to make them both sterile and non-pyrogenic. Terminal sterilization is not an option for AAS bc they are thermolabile, hence the need for aseptic processing.

For the oil, a basic 0.22um filter will make the AAS solution sterile but not necessarily non-pyrogenic bc size of endotoxins can be smaller than a 0.22um membrane. Since endotoxins are negatively charged at a Ph >2, the use of a positively charged 0.22um filter can trap more endotoxins than a non-charged filter can. This will make the solution both sterile and pyrogenic.

For glassware, including vials, the same requirements are necessitated but you can't filter a vial lol. Even with a suitable wash beforehand, autoclaving will only sterilize the glassware but not make it pyrogen free. The FDA and USP define something as non-pyrogenic when there is a log 3 reduction in pyrogens when starting with a minimum concentration of 1000 EU (endotoxin units) or 99.9% inactivation/destruction. Autoclaving cannot accomplish this due to the heat resiliency of endotoxins and pyrogens. A much better option is dry heat 250 deg C for a minimum of 30min. This process has been validated at achieving a log 5 reduction in endotoxins or 99.999% kill rate. Depyrogenation with dry heat acts linearly up until log 3 reduction and biphasically afterwards so you'll need much more time for a higher degree of depyrogenation.

For stoppers since they are not as heat resistant as glass, the best way is through autoclaving. It's beleived autoclaving in the presence of H2O2 is more effective at depyrogenation that autoclaving with just water.

my thoughts? Irrelevant. I do like the part about the .22 filter being the standard for sterility though. I think somebody was saying that...the handle was cdt5074 I think yeah he was saying that before that smart bloke! You should really listen to him more often I think he knows a thing or two.

 

[MisterSuperGod]

Do I have to to do this to you again? Really? Do you get off on it or something? remember .22 filtering is a pharmacy standard for a fucking reason.


Staphylococcus is something that is generally harbored on human Skin. How the fuck it would get in your gear I have no idea. You would have to wipe your ass and stick your hand in your fucking gear, which you might do. So I will continue. Staph is an aerobic Organism, and it cannot survive or grow in oil. So even if you got staph in your vial, how do I say this politely...it would make no fucking difference. It's not gonna make it, just like your dignity from this fucking thread. And let's just say you have staph in your vial or whatever the fuck your gear is in: heat sterilization won't kill the endotoxins it produces even if it kills the staph.

You get staph from...wait...was it aseptic or sterile injection technique. Fuck I forget. You know which one it is right? Help a brother out.

So, being a condescending asshole is how you argue your point?

i think everyone and their brother gets it already.

How many rounds do you need to go before you come to the realization that you're a grown man, arguing and name calling on an internet forum because someone doesn't see your point of view?

 

[colossus25]

my thoughts? Irrelevant. I do like the part about the .22 filter being the standard for sterility though. I think somebody was saying that...the handle was cdt5074 I think yeah he was saying that before that smart bloke! You should really listen to him more often I think he knows a thing or two.

Bro according to you, you filter every shot right? We make gear that stays aseptic all the time all we do is draw and pin. To achieve that one must either use pre-sterile vial (which is great for personal use but not for someone that is sourcing) or use aseptic techniques to ensure their vials are safe. No need to reuse filters or your syringe technique, which works but is just not practical. Yes .22um is the standard and it is what i use, you just went completely off the rails and failed to understand what we were trying to explain to you.

 

[cdt5074]

Unlike you and your wacky ass procedure i don't have to filter every shot. My gear stays completely aseptic, all i have to do is draw and pin. If you want to filter every shot because you dont want to go through the process of makimg your vials aseptic thats on you man.

Filtering every shot is easier than filtering large amounts At the same time what don't you get about that?

I love this, it's your sticking point. See, when someone is mentally inferior or losing an argument, they cling to the same point to try to preserve their credibility and make the other person look stupid. Like trump with clinton's emails. In this case, however, you Don't realize that filtering every shot is easier than filtering large volumes when using syringe filters. Doc can you explain that to your butt buddy? Thanks I owe ya.

 

[colossus25]

Filtering every shot is easier than filtering large amounts At the same time what don't you get about that?

I love this, it's your sticking point. See, when someone is mentally inferior or losing an argument, they cling to the same point to try to preserve their credibility and make the other person look stupid. Like trump with clinton's emails. In this case, however, you Don't realize that filtering every shot is easier than filtering large volumes when using syringe filters. Doc can you explain that to your butt buddy? Thanks I owe ya.

What? I can filter 60ml through a .22 in about 5 minutes lol

 

[colossus25]

Clearly inexperienced at least do it like the ugls and use a caulking gun for crying out loud lol

 

[Docd187123]

Do I have to to do this to you again? Really? Do you get off on it or something? remember .22 filtering is a pharmacy standard for a fucking reason.


Staphylococcus is something that is generally harbored on human Skin. How the fuck it would get in your gear I have no idea. You would have to wipe your ass and stick your hand in your fucking gear, which you might do. So I will continue. Staph is an aerobic Organism, and it cannot survive or grow in oil. So even if you got staph in your vial, how do I say this politely...it would make no fucking difference. It's not gonna make it, just like your dignity from this fucking thread. And let's just say you have staph in your vial or whatever the fuck your gear is in: heat sterilization won't kill the endotoxins it produces even if it kills the staph.

You get staph from...wait...was it aseptic or sterile injection technique. Fuck I forget. You know which one it is right? Help a brother out.

Actually, you're incorrect. Certain strains of staphylococcus can survive in certain oils and remain viable for at least up to two years. Furthermore, dry heat sterilization can deactivate and kill endotoxins. 250deg C for 30min is enough for a log 3 or 99.9% reduction which is FDA and USP guidelines. It's actually been shown to do a log5 or 99.999% reduction.

Also, you mentioned earlier that you reuse the syringe filters until they break. You do realize that they can be reused sure but only up to their rated capacity. Plus, if they dry out between uses they should be discarded as their filtering integrity can be compromised.

 

[cdt5074]

Bro according to you, you filter every shot right? We make gear that stays aseptic all the time all we do is draw and pin. To achieve that one must either use pre-sterile vial (which is great for personal use but not for someone that is sourcing) or use aseptic techniques to ensure their vials are safe. No need to reuse filters or your syringe technique, which works but is just not practical. Yes .22um is the standard and it is what i use, you just went completely off the rails and failed to understand what we were trying to explain to you.

You are so fucking stupid I swear. I circumvent using vials altogether because I FILTER DIRECTLY INTO THE SYRINGE I USE TO INJECT. I don't Store sterile gear in vials...which means i Don't need to buy presterilized vials, or nonsterile vials that. Which makes my methods more efficient and cheaper than whatevercthe fuck it is that you do in your mom's basement.

I really genuinely want to kill you just to make sure you don't reproduce. I feel like I would be saving the world.

 

[wedorecover]

Actually, you're incorrect. Certain strains of staphylococcus can survive in certain oils and remain viable for at least up to two years. Furthermore, dry heat sterilization can deactivate and kill endotoxins. 250deg C for 30min is enough for a log 3 or 99.9% reduction which is FDA and USP guidelines. It's actually been shown to do a log5 or 99.999% reduction.

Also, you mentioned earlier that you reuse the syringe filters until they break. You do realize that they can be reused sure but only up to their rated capacity. Plus, if they dry out between uses they should be discarded as their filtering integrity can be compromised.

You don't want to battle my boy Doc dude is a fucking Encyclopedia I'm trying to get you on who want to be a millionaire

 

[colossus25]

You are so fucking stupid I swear. I circumvent using vials altogether because I FILTER DIRECTLY INTO THE SYRINGE I USE TO INJECT. I don't Store sterile gear in vials...which means i Don't need to buy presterilized vials, or nonsterile vials that. Which makes my methods more efficient and cheaper than whatevercthe fuck it is that you do in your mom's basement.

I really genuinely want to kill you just to make sure you don't reproduce. I feel like I would be saving the world.

Isn't that what i just said you fucking Muppet?

 

[colossus25]

You are so fucking stupid I swear. I circumvent using vials altogether because I FILTER DIRECTLY INTO THE SYRINGE I USE TO INJECT. I don't Store sterile gear in vials...which means i Don't need to buy presterilized vials, or nonsterile vials that. Which makes my methods more efficient and cheaper than whatevercthe fuck it is that you do in your mom's basement.

I really genuinely want to kill you just to make sure you don't reproduce. I feel like I would be saving the world.

I think the heavy metal contamination you're getting from your saw dust gear is fucking up your cognitive reasoning and reading comprehension.

 

[TruEnuff]

Fuckin genius!! We've been doing this all wrong fellas..

You are so fucking stupid I swear. I circumvent using vials altogether because I FILTER DIRECTLY INTO THE SYRINGE I USE TO INJECT. I don't Store sterile gear in vials...which means i Don't need to buy presterilized vials, or nonsterile vials that. Which makes my methods more efficient and cheaper than whatevercthe fuck it is that you do in your mom's basement.

I really genuinely want to kill you just to make sure you don't reproduce. I feel like I would be saving the world.

 

[Docd187123]

my thoughts? Irrelevant. I do like the part about the .22 filter being the standard for sterility though. I think somebody was saying that...the handle was cdt5074 I think yeah he was saying that before that smart bloke! You should really listen to him more often I think he knows a thing or two.

0.22um is the standard for sterility but it is not the standard for non-pyrogenic and in accordance to the federal guidelines you don't know about, a parenteral drug and its packaging material must be both sterile and non-pyrogens.

 

[wedorecover]

I think the heavy metal contamination you're getting from your saw dust gear is fucking up your cognitive reasoning and reading comprehension.

I wish some one would bush this dude in my cell and close the door behind him