Sorry Dr JIM, poor attempt at humor on my part. Your analogy with the cars was actually a big help for a simpleton such as myself, thank you for that.
MESO-Rx
Anabolic Steroids
"Generic" GH ASSAYS
- Thread starter Dr JIM
- Start date
Sorry Dr JIM, poor attempt at humor on my part. Your analogy with the cars was actually a big help for a simpleton such as myself, thank you for that.
I do recall asking a few mates to define what they mean by purity bc the difference bt GH and most other PEDS is simply HUGE and for that reason the distinction is important.
ronin17
New Member
Finally, now I understand Lc\ms. This thread is yet another example of what sets meso apart.
TO CLARIFY the analogy I hope
#1 ) Would be the equivalent of how an AAA QUANTIFIES, a samples content in MG
#2) Would be the equivalent of how an AAA QUALIFIES a samples content as GROWTH Hormone
#3) Although reassembly would be primary difference, all of the above would be required to accurately estimate a samples purity,
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OK so great Dr J then why are "we" not using this better assay, an LC/MS, on all them there samples sir?
Well ok since you asked
COST- the cost of an lc/ms can be as much as 25 X higher than an AAA ($2.5K vs $100 = high end for LC/MS and low end cost of AAA)
And bc of logistical reasons a GH related LC/MS is not reliable enough to QUANTIFY a samples content as in mg.
The fact is many if not MOST biochemists consider an AAA to be the SINGLE ASSAY Gold Standard for PEP analysis!)
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@Dr JIM no sir, no need to post 50 pages of other data related material. I'm am confident you and @mands are doing a stellar job with this testing. At some point, there has to be a level of trust for you and Mands with respect to this testing. I have seen no reason not to trust the info you 2 are posting. Even if you did post the pages of data, I would still be confused with most of it. Your analogy with car parts did help a great deal, but it will take me much longer to understand everything.
@Dr JIM , Based on the info in these posts, am I correct in assuming that Sample 13 is the correct sequence and total number of aminos, but the proportion of GLX is greater in the sample than it should be?
Do you have full data (the 50-100+pages you referenced earlier) on this sample? If so, would you be willing to spend a little more time on it to see how close this sample comes to proper GH?
Honestly, I'm surprised any generics came even this close to what's advertised and am curious to see what your detailed opinion of it would be. I ran various generics for over a year, all that had great Serum results, but my IGF never increased past 160. Thus, I'm a firm believer that the bro science serum test is BS and most generics are just some peptide that give a fake serum result.
After switching to legitimate Seros, my IGF is now 496 on 6iu/day. However, that's quite pricey, so if this sample 13 is worth a damn, I might start cutting my sero's half and half with this product.
Thanks,
JC
There is no peptide that will raise GH serum scores at the 2.5-3.5 hour mark.
mands
Ok, so it's "GH" but it isn't structured correctly or is inactive, or has some other reason for being useless in the body but still elevates serum GH levels when testing at the ~3hr mark
I've seen studies that show excessive amounts of glycine can cause GH serum levels to increase.
mands
@ProfessorX could you post some of your test that you've done on GH? It would be beneficial to all at MESO to see all types of tests.
Thanks you!
mands
Thanks you!
mands
Jim, I have my summoning kit. Ready when you are!Yea but I'll need some help locating that MANDS fella
Yes
Jim was asking for this information:
Proteomic Profiling
Protein characterization
Up to 50,000 sequencing events. Includes UPLC-MS/MS on Q-Exactive Orbitrap via unbiased, data-dependent acquisition, inclusive of sample preparation and parallel multi-enzyme digestions using six enzymes, sequence library searching, relative quantitation and reporting via Proteome Cluster.
Intact protein mass measurement, fragmentation and sequencing by UPLC-MS/MS on Q-Exactive Orbitrap, 90 m, deconvolution of up to 20 mass envelopes
Ill post these test up
I have some SIMEC results, but they are only an assay sheet
Pharmacom Helper
Subscriber
Hello Mands, Frank waiting for results of chromo of our GH, he will answer as soon as he will receive
Chromo?
An Amino Acid Assay has already been posted, how about Pharmacon comment on the QUANTITATIVE aspect of THOSE RESULTS first?
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Pharmacom Helper
Subscriber
I still hope that it will happen before the apocalypse)
Just some added info
I mentioned before that some were "hung up" on purity
TP mentioned it
API (Active Pharmaceutical Ingredients) "Raws" - "Powders" can be tested for "purity"
LC/MS/MS - 8.8mgs per 10mgs (88% purity)
rHGH
The biological effects, purity, and potency of a drug is governed by the chemical structure of the drug for both traditional drugs and biopharmaceuticals. Standard analytical methodologies used for structural analysis of conventional drugs are, however, inadequate for complete characterization of protein-based products.
Two main reasons for this inadequacy are the large molecular size and conformational flexibility of protein-based drugs. The large molecular size hinders the possibility to detect, for example, replacement or chemical modification of a single amino acid residue or a change in a single glycosylation site. These alterations of the biomolecule structure, however, may lead to subtle changes of the molecule conformation resulting in significant changes in the pharmacological properties of the product.
Additionally, the wrong choice of manufacturing conditions or formulation may lead to improperly folded polypeptide chains which are biologically inactive. Hence, further methodologies capable of analysis of the protein conformation are needed.
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