janoshik
Subscriber
Heck he has yet to cite one comment of mine that is "far fetched or simply untrue".
But Ill ask again cite these falsities "Jano"!
"The articles I've cited only provide a reason why glycine
is either being added OR not removed from generic GH products anything else is CONJECTURE."
Already reacted to this stupidity.
"LC/MS is not reliable enough to QUANTIFY a samples content as in mg."
Retarded stuff. Althought MS is rarely used because it simply is NOT NECESSARY and provides few benefits compared to UV detection it certainly is accurate enough.
"The fact is many if not MOST biochemists consider an AAA to be the SINGLE ASSAY Gold Standard for PEP analysis!)"
for unknown peptides/proteins. In research. Is HGH unknown molecule? NO.
Is testing anything without a standard retarded? Yes.
And you've stated you don't need a standard.... What a pity the REAL doctors, pharmaceutists and biochemists all need them.
HPLC and immunoassays are the gold standard in pharmaceutics and medicine.
"AAA QUANTIFIES GH quite accurately with a margin of error of around 5%, IF performed correctly! "
Hey, this one is correct, but I feel to point out that you yourself had admitted that it has bigger margin of error than HPLC analysis! Good job JIM!
"GH PURITY IS
- the Amino Acid sequence,
- the correct distribution like 3 Valines, followed by 2 Cystines, then a Tryptohan
- the total number of AA which should be 191
- the absolute number of individual AA such as; Cystine 6, Valine 13, Tryptophan 4
- the correct folding pattern such as between AA # 23 and 94"
What a load of horseshit. Go back to high school.
"trypsin cleavage LC/MS to (the most reliable single "purity" assay)"
what a load of horseshit, trypsin and MS have nothing to do with purity assay (well, MS can have, but it generally is troublesome with proteins)
also, just a few lines above you stated LC/MS was not reliable, Mr. JIM, make up your mind or you look more and more like a fool
"An LC/MS cuts GH, or in this comparison the vehicle at specific points, "cystine bridges"
So are you telling me that MS disrupts disulfide bonds specifically? LOL
"HPLC can NOT determine PURITY."
LOL
"HPLC evaluates the AVERAGE MW "
LOL LOL LOL
"Finally with a margin of error of around 0.1% it's considerably less sensitive and/or specific than an AAA!" (talking about HPLC)
HAHA. So a few lines up you are talking that AAA can be inaccurate to up to 5% and here you say that HPLC having margin of error 0.1% is much more than 5%
0.1% > 5%
GOOD JOB MR. JIM
Not even going to go further back.