Laboratory testing of AASs, GH, peptides...

[mercury]

Thanks for the reply, however I would like for him to express what he is comfortable with as you don't appear to see where I am going with this.

sorry the first sentence was not meant for you, I do not know where it came from.

 

[colossus25]

Take it from a guy that has been around these boards for quite some time, no one wants you to comment or even cares what you have to say.

If your done, then be done with it.

Ya know bud, even if the new proposed testing lab doesn't work out, it doesn't change anything you have done here in the last few days. You have really hurt yourself with the shady tactics, lies and all around bullshit.

You remind me of a jealous girlfriend that is about to lose her man to someone better that will stop at nothing to get him back... but, it's too late.

Good luck bouncing back from this one. You did it to yourself.

I care about what he has to say lol and he is absolutely right, you are done on meso. Nobody is going to take you seriously again merc. I remember when i first joined and i used to see you do labmax test on all labs and everyone on here respected you. You flushed all that down the toilet then minute you started this shit. Insulting respected members and trying to discredit people with falsehoods. Now you're gonna have to look back on that as the glory days because that time is long gone.

 

[Analyzer]

@Analyzer I believe there is still some confusion about this statement. Can you explain a little more about external and internal standards for the meso group.

I do know from reading "external standards do not correct for losses that may occur during preparation of the sample, such as extraction, centrifugation, evaporation, etc."

mands

By definition, external standard is a compound present in a standard sample of known concentration and volume which is analysed separately from the unknown sample under identical conditions. In other words, separately test the standard, the sample and compare detector responses (areas under the peaks). In accredited conditions, certified reference material of the compound which is tested is used, I used chemically similar compound with known relative response factor. From the known weights of sample and standard you can calculate how much of target compound is present in the sample. Repeating analysis several times allows you to determine confidence interval at certain level.

In internal standard method, chemically similar compound is added to the sample, and they are analyzed together, in one run. This prevents deviations caused by loses you mention. In analyses I do, this is not usually necessary.

 

[wedorecover]

@Ryno1980

 

[Analyzer]

@Analyzer can I play devil's advocate and ask if you are willing to test for other substances that may possibly be controlled.

Or is this STRICTLY for AAS only?

If you mean narcotics, the answer is definitely NOT.

 

[wedorecover]

If you mean narcotics, the answer is definitely NOT.

That's what I wanted to hear

 

[master.on]

@Iron Vett , thank you for the question.
Since I am not accredited laboratory, it is quite hard to get certified reference material. Hence I use raws I tested in the past. Because they usually contain some impurities, I need to purify them to get the standard.

The most convenient way to do so is to use flash chromatography. It is a separation technique commonly used to purify organic compounds in research laboratories. It works on similar principle as HPLC. The main difference is quantity of material you can separate. HPLC usually works with micrograms, preparative flash chromatography works with up to gram quantities.
Preparative chromatography uses bigger columns and higher solvent flow rates. After the target compound is separated from the impurities (you see them as signal coming from UV or ELSD detector), it is separately collected, solvents are evaporated, the compound is dried to constant weight and the purity is confirmed by HPLC and/or GC. Then I use it as a standard.
To get an idea how such chromatography looks like: Biotage - Flash Purification

It is not accredited practice, but the results you get are the same. It is broadly used in research where you often work with new compounds which certified reference materials simply does not exist.

By definition, external standard is a compound present in a standard sample of known concentration and volume which is analysed separately from the unknown sample under identical conditions. In other words, separately test the standard, the sample and compare detector responses (areas under the peaks). In accredited conditions, certified reference material of the compound which is tested is used, I used chemically similar compound with known relative response factor. From the known weights of sample and standard you can calculate how much of target compound is present in the sample. Repeating analysis several times allows you to determine confidence interval at certain level.

In internal standard method, chemically similar compound is added to the sample, and they are analyzed together, in one run. This prevents deviations caused by loses you mention. In analyses I do, this is not usually necessary.

Crucial questions for credibility:

1 When will you post equipment pics?

2 Does the equipment manufacturer provide data libraries for substance identification?

 

[Bradly]

oh lawd

 

[master.on]

Crucial questions for credibility:

1 When will you post equipment pics?

2 Does the equipment manufacturer provide data libraries for substance identification?

So?

 

[Sampei]

Where are you based?

Hello everyone; thank you @mands for introducing me!

I want to offer my service to this community. I can test almost any kind of sample including AASs, SARMs and peptides for identity, purity and content.
I am researcher with rich experience in development of analytical methods and I can provide these analyses at highest level of quality, utilizing various spectroscopic and chromatographic methods.
Analyses are provided for reasonable price in range 40-70 USD per AAS/SARM sample and 100-200 USD per peptide/GH sample, depending on type of analysis and number of samples. If you have any questions, feel free to ask me.

In case of interest, please PM me.

 

[Analyzer]

Crucial questions for credibility:

1 When will you post equipment pics?

2 Does the equipment manufacturer provide data libraries for substance identification?

1. Here you are:


2. Yes, there are Wiley/NIST libraries included.

 

[mands]

Where are you based?

Answered this in PM.

mands

 

[master.on]

1. Here you are:
View attachment 67732 View attachment 67733 View attachment 67734

2. Yes, there are Wiley/NIST libraries included.

Nice machine
the 5975c is a Gas Chromatograph/Mass Spectrometer

Are you going to use HPLC as well?
Do you have one? (pics plz if so)

GS/MS is particularly suitable for substance identification and data libraries are highly desirable

For example take a look at this result from ecstasydata.org
Note
Melatonin by library match only. The product was sold as methenolone but it is definitely ruled out. This tablet does not contain methenolone. Received five identical tablets.
EcstasyData.org: Test Details : Result #3082 - Primbolan Acetate

This goes a step beyond as not only they determined the tab doesn't contain any Methenolone Acetate (oral Primo) but identified it is Melatonin instead.

This is especially interesting as we all have been wondering what substances are present in impure powders? Are they harmful?

Once you determine which substances are there,
How are you going to determine purity percentage? HPLC, UPLC, other?

Again, nice machine.

 

[Analyzer]

Nice machine
the 5975c is a Gas Chromatograph/Mass Spectrometer

Are you going to use HPLC as well?
Do you have one? (pics plz if so)

GS/MS is particularly suitable for substance identification and data libraries are highly desirable

For example take a look at this result from ecstasydata.org
Note
Melatonin by library match only. The product was sold as methenolone but it is definitely ruled out. This tablet does not contain methenolone. Received five identical tablets.
EcstasyData.org: Test Details : Result #3082 - Primbolan Acetate

This goes a step beyond as not only they determined the tab doesn't contain any Methenolone Acetate (oral Primo) but identified it is Melatonin instead.

This is especially interesting as we all have been wondering what substances are present in impure powders? Are they harmful?

Once you determine which substances are there,
How are you going to determine purity percentage? HPLC, UPLC, other?

Again, nice machine.

The third picture is autosampler of Agilent 1290 HPLC system.

You are right, I use both GC and HPLC to see how pure the samples are. All the way, this result is relative, since I would need standards of all impurities to know their exact amounts. Even if there is no impurity visible in these chromatograms, it still may be contaminated with small quantities of inorganic salts or other molecules.

 

[colossus25]

Nice machine
the 5975c is a Gas Chromatograph/Mass Spectrometer

Are you going to use HPLC as well?
Do you have one? (pics plz if so)

GS/MS is particularly suitable for substance identification and data libraries are highly desirable

For example take a look at this result from ecstasydata.org
Note
Melatonin by library match only. The product was sold as methenolone but it is definitely ruled out. This tablet does not contain methenolone. Received five identical tablets.
EcstasyData.org: Test Details : Result #3082 - Primbolan Acetate

This goes a step beyond as not only they determined the tab doesn't contain any Methenolone Acetate (oral Primo) but identified it is Melatonin instead.

This is especially interesting as we all have been wondering what substances are present in impure powders? Are they harmful?

Once you determine which substances are there,
How are you going to determine purity percentage? HPLC, UPLC, other?

Again, nice machine.

Lmaoo after all the shit you talked now you're sucking up. Hahahahaha the irony

 

[master.on]

1. Here you are:
View attachment 67732 View attachment 67733 View attachment 67734

2. Yes, there are Wiley/NIST libraries included.

The third picture is autosampler of Agilent 1290 HPLC system.

You are right, I use both GC and HPLC to see how pure the samples are. All the way, this result is relative, since I would need standards of all impurities to know their exact amounts. Even if there is no impurity visible in these chromatograms, it still may be contaminated with small quantities of inorganic salts or other molecules.

To gain further credibility:
Can you please describe us the testing process with detailed steps?
plz describe manual actions (i.e. dissolving in acetonitrile) and machine-related steps (i.e. changing settings, entering data)

Shouldn't be too bothersome of a requirement
If anything, it helps you write down a checklist
if you don't have one already.

Lmaoo after all the shit you talked now you're sucking up. Hahahahaha the irony

I don't come to Meso with any agenda.
At worst, I can only be accused of making some idiot posts
but that was just for entertainment sake, to make guys loosen up and chill with humor.

 

[colossus25]

To gain further credibility:
Can you please describe us the testing process with detailed steps?
plz describe manual actions (i.e. dissolving in acetonitrile) and machine-related steps (i.e. changing settings, entering data)

Shouldn't be too bothersome of a requirement
If anything, it helps you write down a checklist
if you don't have one already.


I don't come to Meso with any agenda.
At worst, I can only be accused of making some idiot posts
but that was just for entertainment sake, to make guys loosen up and chill with humor.

Lmaoo ill give you that at least you didn't have an agenda.

 

[Docd187123]

To gain further credibility:
Can you please describe us the testing process with detailed steps?
plz describe manual actions (i.e. dissolving in acetonitrile) and machine-related steps (i.e. changing settings, entering data)

Shouldn't be too bothersome of a requirement
If anything, it helps you write down a checklist
if you don't have one already.


I don't come to Meso with any agenda.
At worst, I can only be accused of making some idiot posts
but that was just for entertainment sake, to make guys loosen up and chill with humor.

No no no. At BEST you make stupid posts and threads. At worst you waste oxygen for the rest of humanity and the animal kingdom.

 

[master.on]

No no no. At BEST you make stupid posts and threads. At worst you waste oxygen for the rest of humanity and the animal kingdom.

fuck you doctor wannabe

insulting is easy
at least try to explain why do you think the thread is stupid (it isn't)
you're no real MD, by the way

 

[wedorecover]

So anyone gonna jump