connoristiny
New Member
u put on the vial directly on stove? What temp?Oh you’re boiling water. I thought you were just putting the vial on a flat top stove.
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u put on the vial directly on stove? What temp?Oh you’re boiling water. I thought you were just putting the vial on a flat top stove.
Thx for the info. So you prefer PTFE over PDVF?Pic of the filters I use
- PTFE
Polytetrafluoroethylene (PTFE) membranes are chemically resistant to nearly all solvents, acids and bases. The membrane has low extractables and good thermal stability. PTFE is hydrophobic and requires pre-wetting prior to use with aqueous solvents.
Membrane: HPLC certified PTFE, with polypropylene support
Use with: Aggressive solvents, strong acids, alcohols, bases, aromatics
Don’t use with: Aqueous samples without pre-wetting (causes high back-pressure)
Sterilization: Syringe filters can be sterilized by autoclave 121°C at for 15 minutes.
Applications: Filtration of aggressive organic or highly basic solutions, transducer protectors.- PVDF
Polyvinylidene difluoride is a hydrophilic membrane that is suitable for most general biological sample filtration. It has a broad chemical compatibility and is a low protein binder and so a good choice where high protein recovery is required.
Membrane: HPLC-certified PVDF
Use with: Alcohols, weak acids, proteins, peptides and other bio-molecules
Don’t use with: Some strong acids, bases, esters, ethers or ketones.
Sterilization: Syringe filters can be sterilized by autoclave at 125°C for 15 minutes.
Applications: General biological filtration, filtration of samples for high protein recovery.
thanks for the heads up how long does one whatman filter last typically?Just make sure it's the right kind of material used for the filter, it HAS to be the PVDF filter. Otherwise the BB/BA in the gear will dissolve some of the filter membrane and you will potentially be injecting weird plastic polyvinyl shit without knowing it.
I haven't been able to find any filters I would trust on Amazon that's why I just pay a little extra to get the whatman name brand filters.
@connoristiny
When it comes to sterility of your gear and injecting it into your body it's worthwhile to spend a couple of extra bucks to make sure you have the appropriate materials.
Fuck using syringe filters takes way too long,bottle top filters and a hand pump for less then $70 you can filter 1000ml in less then five minutes.Pic of the filters I use
- PTFE
Polytetrafluoroethylene (PTFE) membranes are chemically resistant to nearly all solvents, acids and bases. The membrane has low extractables and good thermal stability. PTFE is hydrophobic and requires pre-wetting prior to use with aqueous solvents.
Membrane: HPLC certified PTFE, with polypropylene support
Use with: Aggressive solvents, strong acids, alcohols, bases, aromatics
Don’t use with: Aqueous samples without pre-wetting (causes high back-pressure)
Sterilization: Syringe filters can be sterilized by autoclave 121°C at for 15 minutes.
Applications: Filtration of aggressive organic or highly basic solutions, transducer protectors.- PVDF
Polyvinylidene difluoride is a hydrophilic membrane that is suitable for most general biological sample filtration. It has a broad chemical compatibility and is a low protein binder and so a good choice where high protein recovery is required.
Membrane: HPLC-certified PVDF
Use with: Alcohols, weak acids, proteins, peptides and other bio-molecules
Don’t use with: Some strong acids, bases, esters, ethers or ketones.
Sterilization: Syringe filters can be sterilized by autoclave at 125°C for 15 minutes.
Applications: General biological filtration, filtration of samples for high protein recovery.
Fuck using syringe filters takes way too long,bottle top filters and a hand pump for less then $70 you can filter 1000ml in less then five minutes.
really it takes forever to filter it? how long does it take with the whatman syringe filter?Good call @Jurgen I knew there was one other filter type that was good thank you for posting that.
You can easily filter 10 vials of 10mL with 1 filter, some people do alot more like 500mL, they sometimes break if you get a cheap version or push too hard.
It's a total pain in the ass, and if your doing anything above the minimum amount of homebrew @warlordishere has the right idea for sure.
I mean it's relative, like 5min for a vial, you just have to push on the syringe the whole time, it's just tedious.really it takes forever to filter it? how long does it take with the whatman syringe filter?
word which brand u use?everything is 1 time use unless autoclavable and can re sterilize. just use syringe filter if doing 1 vial and already pre filtered
yeah that sounds annoying but is what it is I guess. cant be much worse than drawing gear with a 30g slin pin which Ive done before lolI mean it's relative, like 5min for a vial, you just have to push on the syringe the whole time, it's just tedious.
I only filter if I get a floater or if there’s some stopper in the vial with a lot left in it. If I was brewing or filtering a lot then I would probably invest in that. But I’ve maybe filtered a total of like 4 vials in the last 2 years lol.Fuck using syringe filters takes way too long,bottle top filters and a hand pump for less than $70 you can filter 1000ml in less than five minutes.
I understand completely especially since premade gear is so fucking cheap from qsc but I prefer making my own gear since I can control the the mg/ml using solvents and oils I like.I only filter if I get a floater or if there’s some stopper in the vial with a lot left in it. If I was brewing or filtering a lot then I would probably invest in that. But I’ve maybe filtered a total of like 4 vials in the last 2 years lol.
Or the North Pole?do u live at high elevation?
Unless you live in an igloo and its -10 degrees in your house you just didn’t heat it up enough or didn’t heat evenlyno cap my damn bold cyp literally crashed in my freakin syringe during the injection. how tf am I supposed to use this crap?
Use a hair dryer to warm that sucker back up and you should be fine.no cap my damn bold cyp literally crashed in my freakin syringe during the injection. how tf am I supposed to use this crap?
I haven’t yet. I’ve been using my coffee maker warming plate. Usually 5-10 minutes depending how full the vial isu put on the vial directly on stove? What temp?
Will we get primo 200 mg in EU warehouse?