Jut because it works in a laboratory setting doesn’t mean it’s the right way to do it. You’re quoting an article that was written on how to provide dental work in the field, in a third would country. Yes it can kill bacteria, that’s not the point, the point is the QUALITY. You’d have to follow those sesifications to the letter to get the results they got but even then those tests for a study were still don’t in a lab my pros. They are not advocating the use of a pressure cooker for lab work, they are stating that in a third world country a pressure cooker would be possible. The only “professionals” you’ll see use pressure cookers are schools where they’re trying to demonstrate sterile techniques but ensuring 100% sterility isn’t nessesary as schools aren’t injecting anything.
The point is this, you are not emphasizing quality and security like you stated in the beginning. Everything has been about quantity; the number of tabs, the number of customers, the number of labels, the number of caps you aren’t making, the number of boards you’re on and the number of people you can expand and reach to.
Also, let’s not forget all of the other questions I asked, the only one you addressed was the gloves. There’s probably a dozen more in there to answer.
So I'll use you words in the form of numbered questions, and my answers corresponding with the same number.
Or I'll make a typo so we will see
Question 1
you're using protexics surgical gloves that cost like $3 a pair but you can't afford an autoclave? Also, they're latex, what are you doing to protect against clients that may have latex allergies for something they are injecting? Protexis makes non-latex gloves, switch.
Next you have a filter set up using a peri pump, how are the lines cleaned and sterilized after each run to avoid cross contamination and maintain sterility?
Answer 1
The lines are actually replaced entirely. The tubing is dirt cheap. All tubing is washed with aloconox solutoin , then rinsed with distlled water, and then bathed in distilled water and Isopropyl alcohol and then steam sterilized.
Question 2
The bottle it's going into has a vent hole haphazardly made into the cap, I can see the burrs left over. That's supposed to be sterile.
Answer 2
The entire cap setup was/is steam sterilized and the underside of the cap is sealed and smooth
Question 3
Next we have a basket with vials just laying in there, why aren't they uniform instead of strewn about, that's how things chip, or at the least grind. Has the basket beensterilized? You mentioned that the vials are put into autoclave bags, where are they? why were the vials not left in the autoclave bags? the sealed bags were sterile, if done correctly, and you've now broken that integrity.
Answer 3
I want to be clear - we use factory sterilized vials to be more efficient that come with both the silica stoppers and the vials themselves. Yes we used sterilization pouches prior when we were still doing it ourselves. We do still sterilize our larger media - flasks / beakers etc. For these we do not use sterilization pouches.The tops are covered with pre treated aluminum foil with sterile gloves and placed in a oven set at 350 F to dry any residual sterile water from the PC. Of course the mesh basket is run through the PC and transferred into the flow hood with sterile glvoes just like everything else.The vials are not clanking around in the basket or grinding ...there is very little movement except being picked up out of the basket and set down to fill. The stoppers are picked up with sterilized hemostats , the tips of which are never touched, and the handles being touched only with sterile gloves, and are placed on the vials after being filled. They are then rubbed with chlorahexidine and finally capped.
Question 4
Then we have all the stoppers laying on the counter, again, how were they sterilized, and why were they taken out of the bag and tossed on the open counter?
Answer 4
The silica stoppers are, rather were as we have transitioned to presterilized vials and stoppers, ran through the PC after receiving a bath in distilled water and alochol. They were removed from the PC (they were placed in a mesh basket to keep off the bottom of the cooker) and placed in the flowhood.
Question 5
For the pressure cooker, I don't see any locking lugs, how is that pressure cooker secured and what's the heating source? Based on the measurements compared to the water and alcohol you have a 18-21L pressure cooker, what temperature, pressure and time are you using to sterilize each load? How many vials are sterilized in each load and how are they arranged inside the vessel?
Answer 5
Our goal temp is 127C at 20PSI and 30 minutes time. The pressure cooker has an interlocking steel seal and holds pressure quite well.
Am I missing anything?? Or is this enough