2,4-DiNitroPhenol [DNP]

Heretic

New Member
Credit to the gods that wrote this... :)

HOW TO NOT FUCK UP DNP:

Since some guys have been playing around and disrespecting DNP and then griping to the forums about the painful results, we need to make this VERY specific and VERY correct so that people won't keep jumping for DNP out of curiosity, or without the willpower they need to operate this respondibly. So here are my experienced guidelines to using it the RIGHT way.

FIRST GUIDLINE: Dosing. Use ONLY 200mg a day for the first four days. I don't care that you don't "feel" anything yet and you wanna bump it up. DNP accumulates in the body, and not "feeling" something means NOTHING. It's there, and it's working (the effect on metabolism begins within two hours of the first dose!). Four days will let you test your tolerance: do you have an allergy? Does it give you a rash? etc.
Only after those four days do you bump it up, by 200mg a day. The average dose is 400-600/day, and more than that gets a little severe. A full gram is the highest dose I've heard anyone use. I've used that much, and it's hell. I like to stay around 600 a day, which is HOT but safe and effective. Take caps even hours apart through the day, ending about 4-5 PM.

SECOND GUIDLINE...How to eat on DNP. This is purely personal experience, because some guys like to carb-deplete *before* using DNP (then eat carbs as usual while on), and other guys like a low-carb approach throughout. Both are fine. Using DNP is the only time that fructose is a desireable cutting carb, because it keeps the liver replentished. That reduces lethargy and spares muscle.
Be aware that eating high-carb foods WILL increase the heat sensation within an hour, and last about 2 hours. That means don't eat carbs before bed unless you want those night sweats to be even WORSE.
Personally, I ate whatever the hell I wanted! IHOP, chinese, fajitas...Yes, I burned hot, but I still lost 1.5 pounds every 2 days. Keep protein HIGH for muscles' sake, and try it yourself.

Foods I suggest including:
Blueberry yogurt. Blueberries are excellent antioxidants, and yogurt cultures help with digestive function, gas, and stool consistency (disgustingly soft stools are common during DNP).
Oregano-based foods. Oregano is perhaps one of the most potent antioxidants around,a nd one spoonful counts as a vegetable serving. See this article
Pineapple - I've found that pineapple helps alleviate those "DNP Blues". The fructose helps, and pineapple enzymes aid in protein digestion.
V8 - one 12-ounce can supplies six servings of veggies, concentrated as an excellent source of antioxidants, lycopene, and recovery of electrolytes.
Oatmeal - high-fiber foods are necessary. You'll find out why around, oh, day 5 or so. Trust me.


THIRD GUIDELINE...Supplements and DNP. I suggest:
ECA - DNP is not a stimulant. To keep energy high and aid in fat loss, use an ECA. Some advisors suggest that regular ephedrine is preferable to norephedrine because of the more direct "hit" of energy.
Prohormones - perfectly fine on DNP. I used 1-AD just to help keep strength and muscle up, and it worked fine. No problems here. You won't GROW muscle on DNP, but it'll help with strength and protection.
Obvious stuff - multivitamin, ZMA, etc.
Biotest PowerDrive - No, I'm not pimping Biotest. But PowerDrive is an excellent pre-workout mixture that actually works. Plus it's low-carb (only 15 calories total), so it won't cause carb-heat in the middle of your workout.

Antioxidants - I'm giving my own personal list, and why I use them:
Alpha Lipoic Acid - aids in fat management and blood sugar, and an excellent antioxidant.
Grape seed extract
Syntrax Radox
Green Tea
Inositol - mood enhancement, antioxidant, and muscle support. 1 gram/3x day
Ellagic acid - protects cell DNA/RNA from damage by free radicals, and may even atack cancerous cells. 400mg/twice a day
Fruit antioxidants - beyond-a-century's powder of high-potency natural fruit anti's. 1 gram, 2-3x day.
Trimethylglyceine - antioxidant, helps move fat and blood lipids into the liver and out of the body. 500mg, 2x day.
Vitamins E and C

Supplements NOT to use:
Any medications that suppress energy. No allergy meds, antidepressants, muscle relaxers, or beta blockers. DNP will have you low as it is; don't worsen your body's energy by taking something that suppresses you further.

DRUGS - Sheesh, you'd think I wouldn't have to mention this, but two idiots in particular (right here on this forum) recently affirmed that some people still just don't get it. NO alcohol (not even "moderate"), NO ecstasy, NO GHB, etc. If you don't have the willpower to forego these habits, DNP is not for you.

Syntrax Swole - a personal discovery. I tried Swole while on DNP...once. Two hours of hell, feeling inside-out.

FOURTH GUIDELINE...working out on DNP. Keep lifting short, 30-40 minutes. DNP works very well, causing your body to use 150% or more the calories per action you'd normally use. That means DON'T try to repeat your usual workouts. Drop to moderate weights, 8-12 reps, not to failure, and with plenty of walking rest between sets. You are NOT going to grow muscle on DNP, so don't use your usual heavy routine. Since DNP can cause light-headedness and heat dizzyness, you have my permission to skip squats in favor of leg presses this time.

Cardio is a controversial one. My advice - do NOT do cardio on high doses of DNP (600mg or more). It's dangerous and counterproductive. Below that amount, some cardio is fine, but keep it to 20 minutes and not at full-gallop. Remember, DNP will drain water from your quickly, causing you to leech out minerals, vitamins, and salts. Don't overdo it.

During exercise, consume at least 1 liter of water per 30 minutes of work, whether you're thirsty or not. DNP is evil in the way it blunts thirst, while at the same time doing the cruel trick of bloating your body with water WHILE dehydrating you from water in your organs. MAKE yourself drink. Always folllow DNP exercise with antioxidants, carbs, and this is a good time to use your multivitamin.

Don't feel embarrassed about poor workouts. Just this morjning I did a workout with a whopping nine sets (wimp!) before calling it quits. Listen to your body, and let it tell you when enough's enough; don't guage workouts by what you *usually* can do otherwise.

Here's my research. This is AMAZING! Not only has not a single test found it to be carcinogenic, but test after tyest after test find that DNP actually ATTACKS cancer cells, and helps anti-cancer medications work better, and helps anti-leukemia medications work without destroying cell DNA, and suppresses tumor growth by 20-50%. The summaries are all right here, friends. Karma me up!

DNP is Ames negative, and does not promote tumors. See for yourself at http://toxnet.nlm.nih.gov/

http://www.epa.gov/ttn/atw/hlthef/dinitrop.html (2,4-Dinitrophenol | Technology Transfer Network Air Toxics Web site | US EPA) reports on health risks. While there have not been human studies, animal studies found no cancers caused by DNP administration. It is considered a toxin because it causes nausea, sweating, and weight loss.

Cyberiron.com reports on halth risks from external exposue. In other words, don?t get it in your eyes, or on your skin if you?re allergic. Pretty elementary stuff.

http://www.ebec2000.com/abstracts/056.htm This animal study documents a 64% increase in metabolism. "These findings confirm that DNP effectively increases metabolic rate..." Duh.

Biosource A PDF file about an antidote to DNP.

http://www.boehringer-ingelheim.es/...glesa/cap13.htm finds that DNP did not activate liver enzymes (MAT) associated with liver damage

"Comparative study of toxicity of 4-nitrophenol and 2,4-dinitrophenol in newborn and young rats." Koizumi M, Yamamoto Y, Ito Y, Takano M, Enami T, Kamata E, Hasegawa R. Division of Risk Assessment, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. This study found that DNP can induce death in overdosed amounts, but that up to that point no toxicity was evident, nor were there any abnormalities in physical development.

"Phenol toxicity and conjugation in human colonic epithelial cells." Pedersen G, Brynskov J, Saermark T. Dept of Medical Gastroenterology, Herlev University Hospital, Copenhagen, Denmark.. This study found that DNP has a toxic effect on cells of the colon, with "toxic" defined in two ways: first, it interfered with metabolism (this we know?it?s the intended effect of DNP users!) and second, it interfered with bowel inflammation (not a health risk. This is caused by osmotic effect, with the worst results being softened stools and gas).

"Mechanisms of bacterial resistance to macrolide antibiotics." Nakajima Y. Division of Microbiology, Hokkaido College of Pharmacy, 7-1 Katsuraoka-cho, Otaru, Hokkaido 047-0264, Japan. This study found that antibiotic-resistant bacteria could be thwarted with DNP. "the extent of the accumulated drug in a resistant cell increases as much as that in a susceptible cell in the presence of an uncoupling agent such as?2,4-dinitrophenol (DNP)."

"Absence of Crabtree effect in human melanoma cells adapted to growth at low pH: reversal by respiratory inhibitors." Burd R, Wachsberger PR, Biaglow JE, Wahl ML, Lee I, Leeper DB. Departments of Radiation Oncology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA. Check this out?DNP actually helps make melanoma tumors easier to attack by increasing ratio of oxygen consumption to lactic acid production, while glycolysis remains the same. "Therefore, tumor acute acidification and oxygenation can be achieved by exposure?"


"New insights in the cellular processing of platinum antitumor compounds, using fluorophore-labeled platinum complexes and digital fluorescence microscopy."
Molenaar C, Teuben JM, Heetebrij RJ, Tanke HJ, Reedijk J. Department of Molecular Cell Biology, Leiden University Medical Centre, The Netherlands. DNP is used as a control in tests of antitumor cells because it does NOT bind to cell DNA, nor promote tumors, yet its staining abilities enable tracking of the uptake of antitumor drugs.

Specific inhibition of breast cancer cells by antisense poly-DNP-oligoribonucleotides and targeted apoptosis." Ru K, Taub ML, Wang JH. Department of Biochemistry, State University of New York, Buffalo 14260-3000, USA Are you ready for this? DNP actually INHIBITS (!!!) breast cancers! Yes, not only does it NOT promote cancers, it?s being recognized as a cancer-fighter/blocker. "Two membrane-permeable and RNase-resistant antisense poly-2'-O-(2,4-dinitrophenyl)-oligoribonucleotides (poly-DNP-RNAs) have been synthesized as inhibitors of human breast cancer?fluorescence assay indicates that the targeted antisense inhibition by poly-DNP-RNAs leads to apoptosis of SK-Br-3 cells but does not affect nontumorigenic MCF-10A cells. The control poly-DNP-RNAs with random or sense nucleotide sequence are completely inactive." Plain English? DNP can be synthesized as an anti-cancer compound, because tests show that it blocks mutagens but does NOT affect non-mutagenic (healthy) cells, and has no RNA effects on them.

"Heat shock protein induction by certain chemical stressors is correlated with their cytotoxicity, lipophilicity and protein-denaturing capacity." Neuhaus-Steinmetz U, Rensing L. Institute of Cell Biology, Biochemistry and Biotechnology, NW II University of Bremen, Germany. The thermic effect of DNP induces protein synthesis (heat shock protein, or HSP, synthesis). In fact, it?s quite GOOD at it: "ASA, DNP and CCCP induced HSP at lower concentrations than substances with a similar lipophilicity?"

"Comparative effects of the metabolic inhibitors 2,4-dinitrophenol and iodoacetate on mouse neuroblastoma cells in vitro." Andres MI, Repetto G, Sanz P, Repetto M.
National Institute of Toxicology, Seville, Spain. In this study, DNP?s observed effect was an increase in metabolism (duh!), while the other toxins compared to it had harmful in vitro effects but no increase in metabolism.

"Inhibition of uncoupled respiration in tumor cells. A possible role of mitochondrial Ca2+ efflux." Gabai VL.Medical Radiology Research Center, Russian Academy of Medical Sciences, Obninsk. DNP not only does not cause tumors, but it inhibited their respiration by 20-25% compared to controls.

"Amsacrine-induced lesions in DNA and their modulation by novobiocin and 2,4-dinitrophenol." Shibuya ML, Buddenbaum WE, Don AL, Utsumi H, Suciu D, Kosaka T, Elkind MM. Department of Radiology and Radiation Biology, Colorado State University, Fort Collins 80523. In this study, researchers found that DNP abrogates?or disrupts?cytotoxicity in hamsters (using cancerous cells). They expected to find that DNP would interfere with anticancer treatments, but instead found that DNP increased their effects. They state, though, that they cannot claim a proven effect of DNP on anticancer treatments yet, although they do agree that treatment with DNP actually enhanced the effects of the DNA regenerative therapy of anticancer chemotherapy.

"Induction of endonucleolytic DNA cleavage in human acute myelogenous leukemia cells by etoposide, camptothecin, and other cytotoxic anticancer drugs: a cautionary note." Kaufmann SH. Oncology Center, Johns Hopkins Hospital, Baltimore, Maryland 21205. The authors warn that certain anti-leukemia drugs resulted in "extensive DNA degradation." BUT (good ol? DNP to the rescue!), "Preincubation with dinitrophenol abolished the effect?"

"[Dependence of the nature of the action of metabolic inhibitors on ribosomal RNA synthesis in Ehrlich ascites carcinoma cells on cell integrity]" [Article in Russian] Akhlynina TV, Buzhurina IM, Panov MA, Rozovskaia IA, Chernaia NG. DNP actually inhibits the synthesis of RNA in carcinoma cells. In other words, it helps cancerous cells commit suicide by neutering themselves. "Ribosomal RNA (rRNA) synthesis in the intact Ehrlich ascite carcinoma cells is selectively inhibited by papaverin (ED50 = 0.01 mM), 2,4-dinitrophenol (DPN; ED50 = 5 microM), and actinomycin D (ED50 = 0.1 microgram/ml)."

"Autocatabolism of surface macromolecules shed by human melanoma cells." Bystryn JC, Perlstein J. Cancer Res 1982 Jun;42(6):2232-7. This study finds that DNP helps melanoma cells die (autocatabolize) while other cells are unaffected.

http://www.geocities.com/byggdegstor/dnpforside - tons of research, including medical studies. Excerpts:

DNP does not cause liver damage: "Their analyses demonstrate, beyond a doubt, that the liver does not suffer any damage in the course of dinitro treatment." (Biological Study of Dinitro Drugs in Humans By Dr. Jacques Bell. Bell, Jacques. 1939. Etude biologique des produits dinitres chez l'homme. Medecine. 19:749-54. Translation ? 1996 Robert Ames)

Also: "Experimental studies on animals do not show toxic effects of dinitrophenol on the kidney. Anatomical-pathological examinations of animals, even those which died from a massive dose of dinitrophenol, do not reveal any important anatomical changes, except a small degree of cytolysis. Clinical documents are not abundant, but, on the whole, do not seem to demonstrate that dinitrophenol is toxic for the kidneys."

"Dinitrophenol has almost no action on the blood cholesterol. (Grant and Schube)."

"it doesn't seem that dinitrophenol at usual clinical doses is likely to harm the kidneys."

"Dinitrophenol is remarkable for its absence of effect on the cardio-vascular system...dinitrophenol is absolutely devoid of toxicity for the heart."

"Dinitrophenol does not attack cell tissue albumin and does not determine the fat loss to the expense of the muscles, contrary to thyroxine."

"dinitrophenol offers this precious advantage that the cessation of its use at the slightest appearance of signs indicating an imminence of intoxication results immediately in the arrest of those symptoms." (Professor Pouchet)."


Interestingly, one medical theory on a health ADVANTAGE of DNP is that the slight increase in thermogenic temperature simulates the fever a body induces during a viral attack. The body increases itsheat to protect organs but kill viruses, and some theorize that DNP can do the same thing, thus killing viruses in the body. In this mechanism, DNP may have an immune-enhancing effect.
 
Last edited by a moderator:
baughb said:
That seems about what I am going to try as well, but also running EC, as that seems like a good way to keep the fat loss high.

Hopefully I will have the strength/energy to continue some type of workout throughout the weeks on the cycle =)

Back to the questions for conciliator, I am currently running EC 25/200 Twice a day. I also take animal pumps, which is their pre-workout NO, creatine, and caffine mix. Is that too much caffine? I have read plenty of studies about caffine not really having major negative sides, and I don't drink coffee or anything else along those lines during the day.
I think you should be fine. Caffeine is a mild diuretic, so just be sure to stay hydrated.
 
Conciliator said:
There's nothing wrong with a longer, 5 week cycle of DNP. Many people have taken DNP for months at a time. However, you might end up wasting your DNP if you stick to 200mg/day. In 1937, Simkins did an extensive study on DNP in 100+ subjects. He found that there was a rapidly developing tolerance to the drug in the first couple of weeks of use. His solution, as was Cutting and Tainter's, was to simply increase the dosage of DNP to overcome the tolerance and keep fat loss humming along. I think that 200mg/day may be low enough that after 1-2 weeks you will have largely adapted to it. At this point, additional days at 200mg/day would have greatly reduced effectiveness. This may not happen to all people who take DNP, but it's certainly a common phenomenon. For this reason, I'd plan to increase your dosage if after 1-2 weeks you notice few to no side effects.
I'm having trouble wrapping my head around this, so maybe something I'm assuming about DNP is wrong?
-Using DNP Is like riding a bike,(doing cardio) how intense this cardio is depends on how much DNP you take.
-if I ride a bike at a slow rate for 2 hours a day,(take a small amount of DNP) and diet, eventually my body will adapt to the caloric deficit of the Bike and the bike won't burn as many calories,
so If I increase the intensityduration of the bike, I'll start burning calories again that will overcome my body compensating,
-That Minnosota study showed the highest reduction of metabolism is 30%, so pretty much if I take 400mg, no matter what, I'll have a 14% boost in metabolism?
 
Well, I have just past the 10 day point while on DNP. So far, I have lost 14lbs. First 3 days at 200mgs, next 5 days at 400mgs, last 2 at 500mgs. I also have been taking Benedryl daily. Never had any sort of rashes or hives. The first 8 days were a breeze. The weather was chilly out so I was very comfortable in a t-shirt and shorts. The last 2 days were getting tough. The weather warmed up and at 500mgs, I was constantly sweating. Even in T-shirt and shorts I was roasting. At night, I would wake up drenched. Window was open with fan on and I was still sleeping with no covers and sweating. I also started to feel slight flu symptoms. Kept taking my temperature and the highest I ever got was 99.1 degrees. That was only for one day. Now the weather has dropped back down where I am still hot, but not too bad. I think I am going to continue for another 10 days or so.

So far I am definitely happy with the results. I am also very glad that I am doing it during the winter versus during the summer. If it ws hot out, I don't think that I would be able to handle the side effects. With cold weather it is not too bad, but coupling the heat of the DNP and a hot day would be horrible. Even now everything is turning yellow and sheets need to be changed daily. They are soaked in the morning.

I will let you know how the next 10 days will go. :)
 
Phew, just finished reading the full article! I've always read these posts but this is the first time I've ever posted a reply! I'll bet money that I'll start spendin more time in front of my computer now!

A lot of interseting info in here. Was thinkin about taking DNP before and after reading this I think I will give it a try. Only problem is trying to source some, none of my usual sources can (or want to) get their hands on it. Anyone have any ideas if this stuff will be harder to find in the UK (where i'm gonna be for about 8 months)! Any help/ideas are appreciated! :)
 
Conciliator said:
There's nothing wrong with a longer, 5 week cycle of DNP. Many people have taken DNP for months at a time. However, you might end up wasting your DNP if you stick to 200mg/day. In 1937, Simkins did an extensive study on DNP in 100+ subjects. He found that there was a rapidly developing tolerance to the drug in the first couple of weeks of use. His solution, as was Cutting and Tainter's, was to simply increase the dosage of DNP to overcome the tolerance and keep fat loss humming along. I think that 200mg/day may be low enough that after 1-2 weeks you will have largely adapted to it. At this point, additional days at 200mg/day would have greatly reduced effectiveness. This may not happen to all people who take DNP, but it's certainly a common phenomenon. For this reason, I'd plan to increase your dosage if after 1-2 weeks you notice few to no side effects.

Hello Conciliator... it's been quite a while but it's nice to see you still here and providing DNP advice. I am considering another cycle and just read this comment which seems to be a twist on some of your previous comments and advice to me during my 50-60 day cycle.

Interestingly though, despite that I continued to drop weight the whole time, I now think I would like to try a cycle that increases the dosage somewhat so I am trying to decide on the dosage and the cycle length.

I will continue to read and as always your advice is appreciated.
 
DNP seems to be the miracle fat loss 'supplement' for those who are obese and for some reason dont workout and are trying to loose weight by dieting. Bodybuilders have so many fat loss options that maybe dont worth trying DNP.
 
It is a great read. Lots of good examples that emphasize the importance of knowledge. It is amazing how the adverse effects come about -- generally by sheer stupidity.

This is certainly sarcastic, but a couple points from experience:
(1) A jury of your peers is likely NOT what you think it is
(2) The world is comprised, in general, of idiots

Ok, enough sarcasm for a Monday morning -- back to the salt mines
 
As requested, please find attached an Excel worksheet that is a starter for monitoring dnp levels within the body based on dosing at any point throughout the day. To use:

(1) in the date column, put in the date and time when you are taking a cap of dnp
(2) in the corresponding dose column, put in the appropriate amount of dnp POWDER in the cap that you just ingested in step 1

(3) the spreadsheet should monitor / calculate your dnp level at any point in the future. To estimate at some point beyond your last dose, just input the date and time you wish information on, put the next dose at zero, and you should see the dnp level within your body at that time.

Note that this spreadsheet has not been filled out through eternity. I should do pretty well for tracking ~20 doses, after that, you will need to extend the spreadsheet to permit more inputs, AND you must continue to build the calculation cells out to the right. Simply follow the trend already in place and you should be fine. For any requesting assistance, let me know. Unfortunately, I had to shorten the workbook considerably to allow it to fit into meso's attachment regulations. by email I should be able to provide the entire file, and to check others files as necessary.

Hope this helps ... and remember, there is NO substitute for common sense. a model in the hands of someone who doesn't understand the model is DANGEROUS. please make sure you understand the calculations before relying fully upon the model for information.

Best regards,
just one of many neighborhood geeks
 
Not necessarily. I have done a lot of extensive research on DNP 2,4 and 3,6 and still can not find a good source for it. I'm still looking though. This seems to be one of the forums I have found that is Pro DNP rather then saying it kills and makes you blind. Great post, keep up the good work!
 
TheOnlyVenom said:
Not necessarily. I have done a lot of extensive research on DNP 2,4 and 3,6 and still can not find a good source for it. I'm still looking though. This seems to be one of the forums I have found that is Pro DNP rather then saying it kills and makes you blind. Great post, keep up the good work!
What research have you done on 3,6?
 
Last edited:
Hi, new to the forum, I've been reading posts for advice on weight loss and taking Clen+T/3 because I just started taking these to lose the 12lbs. I recently gained. I have been doing a lot of reading on DNP and it seems to be the best for quick fat loss. I am interested in trying this,safely of course, and you seem to be the one to ask regarding this. I'm 26yrs. old, female, 5'6, 140lbs. thanks to my recent weight gain. I have always worked out and eat really well, so this is not due to me being lazy at all, lol. Any advise on dosage for someone like me would be appreciated, I want to be as safe as possible.Thank you! :)
 
The number one danger with any uncoupler chemicals is the excess heat production. Plain and simple. The body has no failsafe for the heat production from the inhibition of ATP synthesis, and as such, fevers can potentially go as high as they want to.
 
The number one danger with any uncoupler chemicals is the excess heat production. Plain and simple. The body has no failsafe for the heat production from the inhibition of ATP synthesis, and as such, fevers can potentially go as high as they want to.
...which makes proper titration of the dose paramount. If you dose it properly, you shouldn't have any problems.
 
...which makes proper titration of the dose paramount. If you dose it properly, you shouldn't have any problems.

Exactly. That's why all these people pining about 1/8th chance of dying (cough BULLSHIT cough) and how it will have irreversible damage are full of crap. You control the dosage, and the level dosage is the only thing that changes the effects. Therefore, if you're not an idiot, you can use it relatively safely (just like any other drug or chemical)
 
Back
Top